Air Chamber Research Articles

Global atmospheric methane uptake by upland tree woody surfaces

Methane is an important greenhouse gas1, but the role of trees in the methane budget remains uncertain2. Although it has been shown that wetland and some upland trees can emit soil-derived methane at the stem base3,4, it has also been suggested that upland trees can serve as a net sink for atmospheric methane5,6. Here we examine in situ woody surface methane exchange of upland tropical, temperate and boreal forest trees. We find that methane uptake on woody surfaces, in particular at and above about 2 m above the forest floor, can dominate the net ecosystem contribution of trees, resulting in a net tree methane sink. Stable carbon isotope measurement of methane in woody surface chamber air and process-level investigations on extracted wood cores are consistent with methanotrophy, suggesting a microbially mediated drawdown of methane on and in tree woody surfaces and tissues. By applying terrestrial laser scanning-derived allometry to quantify global forest tree woody surface area, a preliminary first estimate suggests that trees may contribute 24.6–49.9 Tg of atmospheric methane uptake globally. Our findings indicate that the climate benefits of tropical and temperate forest protection and reforestation may be greater than previously assumed.

Anterior Chamber Air Bubble Dynamics With Decreases in Atmospheric Pressure.

To evaluate the effect of decreasing barometric pressure on intracameral bubble size and intraocular pressure (IOP) in eyes with varying air fills in the anterior chamber. A total of 36 human donor eyes received 30%, 50%, or 90% anterior chamber air fill. The eyes were subjected to decreases in atmospheric pressure down to 750 hPa, equal to 2400 m in altitude, and were repeatedly imaged using anterior segment OCT while IOP was measured continuously. Eyes with 30% air fill initially showed moderate increases in IOP yet rising to an average of 30.83 mm Hg at 850 hPa (mimicking 1400 m altitude) and 42.08 mm Hg at 750 hPa. Eyes with larger air bubbles showed more acute increases in IOP with increases to an average of 47.25 mm Hg in eyes with 50% air fill at 850 hPa and 63.33 mm Hg at 750 hPa. In eyes with 90% air fill in the anterior chamber, IOP readings with an average of 113.42 mm Hg were observed already at 850 hPa, at which point additional pressure reduction was not performed. While severe increases in IOP were observed with decreased atmospheric pressure in eyes with large air bubbles in the anterior chamber, small and moderately sized bubbles seem to allow for travel over modest changes in altitude.

Proposal and Evaluation of Vertical Vibration Theory of Air Caster

Urbanization and human development have increased the exposure of seismic risk. Therefore, engineers need to develop new and more efficient technologies to protect people and objects from the disastrous consequences of earthquakes. Air casters have gained attention and have been utilized in the past decade as effective seismic vibration control devices. Although such active isolation systems perform well in mitigating horizontal input vibrations, they might cause excessive rocking motions, if not designed properly. This fact emphasizes the importance of exploring the vertical dynamic properties of air isolation systems. To gain such an understanding, this research examines and proposes a formula for the vertical stiffness and damping of air caster systems. Theoretical solutions to the vertical stiffness and damping of such systems have been explored. Computer simulations considering fluid-structure interaction have also been performed to understand the dynamic behavior of the supporting air layer. Results have been compared to validate the proposed dynamic quantities within the considered simulation range. It is also concluded that the instantaneous air layer thickness, representing the air chamber pressure, and the bearing inlet flow rate are the key factors in determining the dynamic properties of the air layer. It is concluded that to evaluate the performance of the air caster seismic isolation device and increase the probability that the qualified seismic isolation performance will be exhibited, it is necessary to investigate which parameters are greatly involved in the viscous damping coefficient and the spring constant of amass-spring-damper system equivalent to the air caster isolation system.

Generalized characterization of pipeline systems with air chamber through development of the dimensionless impedance response

ABSTRACT An air cushion chamber is a feasible and efficient hydraulic device to control water hammer for pressurised pipeline systems. The introduction and evolution and hydraulic transient depend on distinct features of pipeline systems such as length, diameter and the property of pipeline extension as physical properties; flowrates, pressure, boundary conditions as hydraulic conditions; and interaction with surge protection devices (air chamber) as hydraulic conditions. Considering all factors simultaneously can be incredibly difficult, even for a simple air chamber pipeline valve layout. This study introduces the dimensionless transfer function and expression for air chambers in dimensionless frequency domain in order to effectively address the water hammer generation and its counteracting processes. To comprehensively characterize hydraulic transients for pipeline systems equipped with air chambers, two representative dimensionless parameters were used. Along the pipeline system, it is possible to develop a frequency-independent expression for hydraulic impedance. A comparison between the developed method and other existing methods (e.g. characteristic method and impulse response method) revealed excellent agreement. Application of the dimensionless parameters to systems with different dimensions and hydraulic conditions shows that the proposed dimensionless parameters can address substantial ranges of real systems.

Design and application of bidirectional soft actuator with multiangle chambers

Purpose This study aims to introduce a novel bidirectional soft actuator as an enhancement to conventional pneumatic network actuators. This improvement involves integrating air chambers positioned at specific angles to improve stability, adaptability and grasping efficiency in various environments. Design/methodology/approach The design approach incorporates air chambers positioned at a 45° angle relative to the horizontal direction at the actuator's terminus, along with additional chambers at a 90° angle. Mathematical models are developed for longitudinal and transverse bending, as well as for obliquely connected cavities, based on the assumption of piecewise constant curvature. Analyses are conducted on output forces, bending characteristics and end contact areas for both transverse and longitudinal ends. Findings The proposed soft actuator surpasses traditional pneumatic network actuators in gripping area due to the inclusion of a diagonal air cavity and a transverse pneumatic network structure at the terminus. As a result, it provides torsion and gripping force in both directions. Testing on a dedicated platform with two variants of grippers demonstrates superior gripping force capability and performance in complex environments. Practical implications Through the design of multiangle chambers, the soft actuator exhibits diverse driving angles and morphological variations, offering innovative design perspectives for industrial grasping. Social implications The design of multiangle chambers facilitates personalized configurations of soft actuators by researchers, enabling tailored angles for specific interaction environments to achieve desired functionalities. This approach offers novel insights into soft actuator design, addressing more prevalent industrial grasping challenges. Originality/value This study introduces a novel soft actuator design that significantly enhances gripping capabilities in comparison to conventional pneumatic network actuators. The incorporation of specific air chamber configurations and mathematical modeling provides valuable insights for the development of adaptable and efficient robotic grippers for industrial and household applications.

Nathanial Henshaw: Not history’s pioneering hyperbaric practitioner

ABSTRACT Clarke R. Nathanial Henshaw: Not history’s pioneering hyperbaric practitioner. Undersea Hyperb Med. 2024 Second Quarter; 51(2):107-113. A widely accepted belief is that Nathaniel Henshaw was the first practitioner of hyperbaric medicine. He is said to have constructed the first hyperbaric chamber where he treated several disorders and provided opportunities to prevent disease and optimize well-being. While there is little doubt Henshaw was the first to conceptualize this unique medical technology, careful analysis of his treatise has convinced this writer that his was nothing more than a proposal. Henshaw’s air chamber was never built. He would have failed to appreciate how its structural integrity could be maintained in the presence of enormous forces generated by envisioned changes in its internal pressure and, likewise, how its door could effectively seal the chamber during hypo-and hyperbaric use. Henshaw would have also failed to appreciate the limitations of his two proposed measuring devices and the toxic nature of one. Neither of these would have provided any quantitative information. The impracticality of his proposed method of compressing and decompressing the chamber is readily apparent. So, too, the likely toxic accumulation of carbon dioxide within the unventilated chamber during lengthy laborious periods required to operate it. Henshaw recommended pressures up to three times atmospheric pressure and durations for acute conditions until their resolution. Such exposures would likely result in fatal decompression sickness upon eventual chamber ascent, a condition of which nothing was known at the time. It would be another 170 years before a functional air chamber would finally become a reality. Henshaw’s legacy, then, is limited to the concept of hyperbaric medicine rather than being its first practitioner.

Hydrodynamic performance of a pile-supported oscillating water column breakwater in front of a partially reflecting seawall

Integrating wave energy devices with breakwaters can offer an innovative and sustainable approach by combining wave power extraction with wave attenuation. The performance of this integrated system in offshore areas is influenced by the unique characteristics of the coastline. In this paper, a semi-analytical solution was developed using the matching eigenfunction method for the oscillating water column device integrated into a pile-supported breakwater in front of a partially reflective seawall. The model was validated through the energy conservation law, the Haskind relationship, and experimental data. Detailed examinations were conducted on the effects of the seawall's reflection coefficients, the distance between the system and the seawall, the wall draft, and the chamber breadth on hydrodynamic performance. Results show that the presence of the seawall significantly influences hydrodynamic coefficients (hydrodynamic efficiency, reflection coefficient, the relative transmitted amplitude, etc.), accompanied by the piston and sloshing mode resonances inside the chamber and the confined area between the system and the seawall. Due to energy dissipated by a partially reflective seawall, the magnitude of those hydrodynamic coefficients is mitigated, together with the piston and sloshing mode resonances inside the air chamber. The cancellation of the sloshing mode resonance inside the confined area is observed for the smaller seawall's reflection coefficient. The maximum and minimum hydrodynamic efficiency occur when the system is arranged at the wave nodes and antinodes of the formed standing wave field. Lower wave reflection and better wave power extraction can be achieved by properly adjusting the chamber drafts and breadths.

Experimental study of air leakage of reinforced concrete panel with cracks

Evaluation of the leakage rate is necessary for infrastructure where hermetic performance holds critical importance. This study conducted a test to evaluate the air leakage rate of cracked reinforced concrete panels. Hollow beam specimens with an air chamber in the web were prepared to provide a relatively simple setup for airtight pressure testing under 200 kPa pressure. While testing, the lower flange of the beam specimen was subjected to tension cracking under transverse loading, and air pressure was applied to the air chamber of the beam to measure the air leakage rate through the flange plate as the magnitude of loading increased. The test variables were the differential air pressure, flange plate thickness, and reinforcement ratio. The test results showed that the leakage rate increased in proportion to the differential pressure. When the reinforcement ratio increased from 0.7 to 1.3 %, the leakage rate decreased by 90 %. When the plate thickness increased from 75 to 100 mm, the leakage rate decreased by 91 %. The analysis of the test results showed that the leakage rate was proportional to the cube of the crack width (R2 = 0.91–0.95), which agreed with existing prediction models. When the crack width at rebar location was used, the existing models showed the best predictions for test results. This result indicates that to accurately predict the air leakage rate of reinforced concrete members, the critical crack width at the location of reinforcement (i.e. the smallest crack width in the member thickness) is more important than the crack width at the member surface.

Numerical simulation and experiment of coal particles separation in flexible air chamber jig

Flexible air chamber jig is a new type of coal sorting and processing equipment with broad application prospects. The separation of coal particles in the jig mainly depended on the gas working cycle, liquid level height and particles looseness. This paper discusses the separation of clean coal and middling coal in this jig with simulation and experimental methods. Numerical simulation of jigger separation process was realized, and the effects of inlet, expansion and exhaust proportion on the water movement and particle separation were investigated based on the liquid level height, bed looseness and percentage of particles. It was verified by the water level detection experiments. The results showed that the model was accurate and the experimental results were consistent with the simulation. The maximum discrepancy between the water levels in the experiment and the simulation was less than 5%, confirming the accuracy of the VOF-DEM (Volume of Fluid-Discrete Element Method) model. The liquid level was significantly affected by the gas working cycles, while the bed looseness was mainly affected by the intake proportion. Appropriate intake and exhaust proportions increased the proportion of particles with different densities. A reasonable gas working period proportion for jigging separation: intake 24%, expansion 27%, and exhaust 24%. In this condition, the proportions of Zone 1′s clean coal and Zone 2′s medium coal is 81.16% and 94.18%, respectively.

Effect of flywheels in pump stations for reducing the volume of air chambers

ABSTRACT The most common and destructive event of unsteady flow in pipes is water hammer. This phenomenon is caused by a sudden change in boundary conditions in pressurized pipes. Unsteady flow in pipelines can lead to a significant increase or decrease in pressure. This pressure generation may cause cavitation, bursting of pipes, and failure of the whole system. One type of equipment that can control both positive and negative pressure phases is a pressurized tank. Implementing such a pressure tank can impose increased costs. In order to reduce the design costs, the combination of a flywheel with the pressure tank can be used. In this study, a transmission pipeline from Zarineh River to Tabriz city is numerically simulated, and the effect of increasing the inertia of the flywheel on the reduction of the pressurized tank volume is investigated. The results show that increasing the flywheel inertia from 125 to 165 Nm2 can reduce the pressurized tank volume from 90 to 75 m3.

Nonlinear Characteristic Analysis of Gas-Interconnected Quasi-Zero Stiffness Pneumatic Suspension System: A Theoretical and Experimental Study

Because of significantly changed load and complex and variable driving road conditions of commercial vehicles, pneumatic suspension with lower natural frequencies is widely used in commercial vehicle suspension system. However, traditional pneumatic suspension system is hardly to respond the greatly changed load of commercial vehicles. To address this issue, a new Gas-Interconnected Quasi-Zero Stiffness Pneumatic Suspension (GIQZSPS) is presented in this paper to improve the vibration isolation performance of commercial vehicle suspension systems under frequent load changes. This new structure adds negative stiffness air chambers on traditional pneumatic suspension to reduce the natural frequency of the suspension. It can adapt to different loads and road conditions by adjusting the solenoid valves between the negative stiffness air chambers. Firstly, a nonlinear mechanical model including the dimensionless stiffness characteristic and interconnected pipeline model is derived for GIQZSPS system. By the nonlinear mechanical model of GIQZSPS system, the force transmissibility rate is chosen as the evaluation index to analyze characteristics. Furthermore, a testing bench simulating 1/4 GIQZSPS system is designed, and the testing analysis of the model validation and isolating performance is carried out. The results show that compared to traditional pneumatic suspension, the GIQZSPS designed in the article has a lower natural frequency. And the system can achieve better vibration isolation performance under different load states by switching the solenoid valves between air chambers.

Design comparison and effects of a chamber structure on wave dissipation

ABSTRACT Among all the ocean energy sources that have been widely studied, chamber structures such as oscillating water column (OWC) technologies are effective and low-cost methods to develop hundreds of wave power generation devices as the disadvantage is its lack of efficiency. Even if the OWC system does not perform any power generation operations, it is a wave-absorbing structure. A large part of the wave energy may be blocked by the structure’s acting chamber or could be dissipated during the interaction of the waves in the air chamber, which affects the actual power generation benefit evaluation. Nevertheless, a better wave attenuation structure may present a relatively poor design of the OWC generator chamber. A series of experiments were conducted with regular and irregular wave conditions to study the effects of energy dissipation caused by a chamber structure under varied barrier and orifice designs.

Designing, modeling and controlling the angular bending of a foldable soft actuator without using a curvature sensor

Soft actuators have recently attracted considerable attention owing to their inherent flexibility and adaptability. Nevertheless, for a soft robot to successfully engage with its surroundings and perform tasks with optimal effectiveness, it encounters a range of obstacles, including the need for precise and skillful movement, the capacity to perceive its own position and motion and the ability to effectively regulate its flexible structures. Researchers have developed techniques to integrate curvature sensors onto flexible devices, enabling them to detect and react to their positions. However, the integration of curvature sensors into flexible structures presents a substantial challenge in the structural manufacturing process. To address these concerns, this article presents a technique for designing, dynamic modeling and controlling the bending angle of foldable soft actuator without the need for curvature sensors. An optimal design for the geometric dimensions of the soft structure utilizing origami concepts to guarantee the requisite bending properties is suggested. A model-based control method that considers both the motion dynamic and the air dynamic is proposed for controlling the angular bending of the actuator. The motion dynamic was developed using the constant volume principle of the elastomer material and the neo-Hookean hyperelastic theory to establish the correlation between the applied pressure and bending angle. This dynamic model incorporates both the hyperelastic material characteristics of silicone rubber and the geometry of the actuator. Soft actuators have variations in the air chamber’s volume during operation and accurately measuring this variation is challenging. In order to tackle this problem, the fuzzy active disturbance rejection controller is used to predict these variations. The controller possesses exceptional position-tracking capability. This control strategy exhibited excellent responsiveness throughout the range of steady-state error values from approximately 1°–2°. Removing the curvature sensor increases the longevity of this soft actuator and promotes the efficiency of the manufacturing process, hence enhancing the practical application possibilities for the soft actuator made from super elastic material.

Numerical investigation of the hydrodynamic properties of oscillating water column air chambers

Abstract The oceans occupy nearly 71% of the Earth’s surface area. The ocean is rich in energy, and wave energy is one of its important components. Therefore, the study of wave energy generation devices is an important issue. In this paper, we analyze the effect of the size of the air chamber structure on the OWC (oscillating water column) wave energy conversion device under the action of unidirectional regular waves. To analyze the performance of the OWC device under real sea conditions, the wave climate at Sanmenxia is used as the experimental parameter. The performance and characteristic response with the variation of incident wave height, air chamber width and front wall thickness, are analyzed. The results show that the incident wave height, air chamber width, and front wall draft have significant effects on the performance of OWC. With the increase of incident wave height, the wave nonlinear effect increases. As the air chamber width decreases, the horizontal surface inside the air chamber undulates smoothly and the standing wave phenomenon disappears.

Modelling of a Floating-Type Hybrid Wind-Wave System with Oscillating Water Column Wave Energy Converters: A Study Towards Floater Motion Reduction

The integration of floating offshore wind turbines with wave energy converters is regarded as a promising solution for offshore renewable energy development. Given the early stage of wave energy conversion technologies and the substantial influence of control methods on overall system dynamics, a faithful aero-hydro-thermo-elastic-servo-mooring coupled model, along with an engineering environment offering high flexibility for control implementations, is essential. To address the requirement, a numerical modeling framework is developed in this study based on Simulink, known for its superiority in control design and implementation, and OpenFAST, which offers a reliable floating wind turbine model. The model incorporates the thermodynamics of the air in chambers, power take-off dynamics, and oscillating water column dynamics. Furthermore, bypass valves are utilized for the wave energy converters to adjust chamber pressure and reduce floater motion, with a control law proposed to regulate the valve opening ratio. A case study is conducted under harsh ocean conditions to validate the model. The numerical results not only demonstrate the feasibility of the model but also underscore the effectiveness of the control law in improving floater motion performance.

Antibacterial Effect of Nitric Oxide on the Causative Agents of Hospital-Acquired Pneumonia (Experimental Study)

The aim of the study was to evaluate the antimicrobial effect of single and repeated nitric oxide (NO) exposure on the major pathogens of nosocomial pneumonia isolated from the sputum of cardiac surgery patients.Materials and Methods. A 24-hour culture of microorganisms from pan-resistant isolates of Pseudomonas aeruginosa, Escherichia coli, Acinetobacter baumannii, and Klebsiella pneumoniae from the sputum of inpatient cardiac surgery patients with nosocomial pneumonia, as well as strains of P. aeruginosa and E. coli from the American Type Culture Collection (ATCC), were exposed to 200 ppm NO (experimental sample) or medical air (control sample) in a sealed chamber for 30 minutes. After a single or 4 repeated gas exposure at 4 h intervals, Petri dishes were placed in a thermostat at 37°C and the results were evaluated at 24 and 48 h or at 12, 24, 36 and 48 h, respectively. Grown colonies were counted using an automated colony counter and recorded as CFU/mL.Results. No growth of clinical isolates of P. aeruginosa and E. coli was observed 24 and 48 h after a single exposure to NO. Growth of A. baumannii was lower compared to controls at 24 h but continued at 48 h. No effect of a single exposure to 200 ppm NO on other microorganisms was observed. After 4 exposures to NO, the growth of ATCC E. coli was not detected, the growth of other experimental strains was significantly lower compared to the control (P<0.05).Conclusion. Our results provide a rationale for the use of multiple intermittent inhalation of 220 ppm NO for the treatment of patients with hospital-acquired bacterial pneumonia.

Numerical Simulation on the Spray Angle of the Dual-layer Hole Nozzle in a Partition Combustion System of the Diesel Engine

To study the effect of the spray angles of the dual-layer hole nozzle on the combustion and emissions performance in the partition combustion system, the in-cylinder spray, mixture formation and combustion processes of the new combustion system were simulated and investigated using AVL FIRE software. The results show that, compared with the variation of the lower-layer spray angles, the change of the upper-layer spray angles has a great influence on the instantaneous heat release rate. The increasing spray angles of the lower-layer holes lead to reduced peak values of the heat release rate in the cylinder. In all the spray angle cases, the first fire area of the cylinder is in the B zone of the combustion chamber. Compared with lower-layer spray angle, the upper-layer spray angle has a greater impact on the airflow disturbance in the combustion chamber. Appropriately increasing the upper-layer spray angle facilitates the mixing of fuel and air in the combustion chamber and reduces the unburnt fuel equivalence ratio. When the spray angles of the upper- and lower-layer holes are 157° and 112°, respectively, the combustion indicates power has the largest value of 12.18 kW. At the same time, the Soot emission is also the smallest, with a value of 0.52 g/kW·h.

A Pilot Study of Aerosolization of InfectiousMurine Norovirus in an Experimental Setup.

Human norovirus is transmitted mainly via the faecal-oral route, but norovirus disease outbreaks have been reported in which airborne transmission has been suggested as the only explanation. We used murine norovirus (MNV) as a surrogate for human norovirus to determine the aerosolization of infectious norovirus in an experimental setup. A 3-l air chamber system was used for aerosolization of MNV. Virus in solution (6 log10 TCID50/ml) was introduced into the nebulizer for generating aerosols and a RAW 264.7 cell dish without a lid was placed in the air chamber. Cell culture medium samples were taken from the dishes after the aerosol exposure time of 30 or 90min, and the dishes were placed in a 37°C, 5% CO2 incubator and inspected with a light microscope for viral cytopathic effects (CPEs). We determined both the infectious MNV TCID50 titre and used an RT-qPCR assay. During the experiments, virus infectivity remained stable for 30 and 90min in the MNV solution in the nebulizer. Infectious MNV TCID50 values/ml of 2.89 ± 0.29 and 3.20 ± 0.49 log10 were measured in the chamber in RAW 264.7 cell dish media after the 30-min and 90-min exposure, respectively. The MNV RNA loads were 6.20 ± 0.24 and 6.93 ± 1.02 log10 genome copies/ml, respectively. Later, a typical MNV CPE appeared in the aerosol-exposed RAW cell dishes. We demonstrated that MNV was aerosolized and that it remained infectious in the experimental setup used. Further studies required for understanding the behaviour of MNV in aerosols can thus be performed.

High Temperature Solid Oxide Electrolyte Fuel Cell Formulation: Non-Steady State Utilization of Fuel and Oxidant

The formulation presented in this paper was developed with the objective of its application to investigate the transient behavior of a high temperature solid oxide fuel cell (SOFC); especially with respect to the non-steady state consumption of fuel and oxidant reactants. Specifically, the chemical species transient amounts, their mole fractions, and total pressures in the cell anode-side fuel- (hydrogen) and cathode-side oxidant- (oxygen of the air) chambers can be predicted under the isothermal condition at a fixed cell-current level. The developed formulation is also capable of predicting the transient mole fraction profiles of fuel (hydrogen) and oxidant (oxygen) in the cell porous anode and cathode, respectively; thus, providing insight into the reactant species' effective transport and their utilization, via electrochemical reactions, in the cell porous electrodes. The presented formulation can be adapted for any fuel and oxidant combinations in any high temperature SOFC.

Effects of gestational intermittent hypoxia on the autoresuscitation response following anoxia-induced apneas in neonatal mice

Introduction: Sudden infant death syndrome (SIDS) is the leading cause of death in infants from one month to one year of age. Smoking is a significant risk factor in SIDS, and smoking probably causes intermittent hypoxia in the fetus. Therefore, we modeled the effects of intermittent hypoxia during gestation in mice to determine if gestational intermittent hypoxia would adversely affect the capacity of neonatal mice to autoresuscitate effectively after exposure to anoxic stimuli. A degraded capacity to autoresuscitate could explain, in part, why infants born to mothers who smoke have an increased risk of dying from of SIDS. The primary objective of this study was to investigate the effects of intermittent gestational hypoxia on the autoresuscitation responses to anoxia-induced apneas in neonatal mice. Our hypothesis was that pups born to intermittently hypoxic dams would struggle more to recover from anoxic apneas — would have impaired cardiorespiratory responses to anoxic stress compared to the control mice born to dams exposed to room air during gestation. Methods: We studied C57Bl/6 dams who previously had one litter to prove their capability to breed and rear pups. Dams were placed in either an hypoxic or room air chamber at approximately embryonic day 5 (E5; fifth day of pregnancy). The fractional inspired oxygen concentration (FIO2) was reduced to 11% for 10 minutes, three times per hour, with 10 minute periods of 21% oxygen between them. Each hour of intermittent hypoxia was followed by an hour breathing 21% FiO2. The room air control chamber was maintained at a constant FIO2 of 21% with inflows of compressed air that mimicked the noise and flow of nitrogen in the hypoxic chamber. The dams spent the entirety of their pregnancies in the environmental chambers. Once the pups were delivered, the cages were placed in room air, housing environments. The pups were allowed to stay with their mothers until approximately post-natal day 8 (P8). On P8, each pup was fitted into a head out plethysmograph to measure respiratory flow using a pneumotachograph. We recorded respiratory activity, ECG activity, and body movements (using a piezo sensitive film). The pups were maintained at ~34 degrees C in the pneumotachographic chamber. We measured the highest respiratory rate, highest heart rate, longest apnea, gasp latency, mean heart rate recovery, and mean respiratory rate recovery. Results: The mean heart rate recovery and mean longest apnea (in seconds) doubled in the mice exposed to gestational intermittent hypoxia compared to pups born to dams exposed to room air during gestation. The mean heart rate recovery and mean longest apnea in gestationally hypoxic pups was 41.0 +/- 13.7 seconds and 22.05 +/- 12.0 seconds, respectively. The mean heart rate recovery and mean longest apnea in normal, room air-exposed pups was 22.4 +/- 11.1 seconds and 10.3 +/- 7.0 seconds, respectively. The longest apnea in gestational intermittent hypoxic pups was significantly longer than in control pups (53.0 +/- 13.7 seconds and 19.1 +/- 15.0 seconds). Pups exposed to gestational intermittent hypoxia also had increased mean respiratory rate recovery times and gasp latency times (start of apnea until the first gasp). The average body weight of hypoxic pups was 4.3 grams while the average body weight for the normal pups was 4.9 grams. Conclusion: Gestational intermittent hypoxia was associated with delayed and prolonged the autoresuscitation responses in neonatal mice following acute anoxic exposure. We hypothesize that this is due to changes in the brain’s serotonin system. We are investigating how intrauterine hypoxia may decrease serotonin and serotonergic signaling pathways as changes in serotonergic function may degrade the capacity of mice to autoresuscitate following anoxic gas exposure. NIH/NICHD. HD100823 and NIH/NICHD. HD 107060. This is the full abstract presented at the American Physiology Summit 2024 meeting and is only available in HTML format. There are no additional versions or additional content available for this abstract. Physiology was not involved in the peer review process.