Medicinal plants, such as mint, contain active ingredients, which, although they are responsible for the therapeutic properties attributed to them, are also responsible for poisoning and adverse reactions that can occur if they are used in inadequate doses, high doses, or for prolonged periods. Many of these plant species have survived for millions of years thanks to their ability to produce substances that protect them from predators or extreme conditions. Even though some of these compounds (condensed tannins, phenols, alkaloids, oligosaccharides, and saponins) can produce a violent and immediate reaction, in most cases, they have a subtle effect that manifests itself with prolonged ingestion. By applying computational chemistry, the structures of bioactive molecules were revealed, representing them three-dimensionally after virtual screening. Therefore, in the project's development, the effect of water stress on the production of flavonoids in the protected mint crop was studied, using the previously measured field capacity and ultimately controlling the irrigation. This was divided into four irrigation application treatments (each 3, 6, 9, and 12 days), evaluating different agronomic variables and through a foliar analysis of the content of flavonoids present in the four treatments under study. The findings of this study, with their significant implications for medicinal plant cultivation, underscore how water stress can affect the production of flavonoids in mint. This knowledge can be directly applied to optimize cultivation practices and enhance the quality of medicinal plants, thereby benefiting the agricultural and pharmaceutical industries.
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