Five Napier grass varieties were evaluated for agronomic performance and yield at Abobo agricultural research center under rain fed condition in Gambella. The experiment was conducted in randomized complete block design with three replications. Data on agronomic parameters such as plant height, survival rate, leaf to stem ratio, node number per plant, internodes length per plant, tillering performance and dry matter yield were analyzed using general linear model(GLM) procedure of SAS, least significant difference (LSD) at 5% was used for mean separation. Combined analysis indicated that tested varieties varied significantly (p<0.05) for survival rate, plant height, leaf to stem ratio, tillering performance, internodes length per plant and dry matter yield. The highest plant survival rate (86.33%) was recorded for Check followed by varieties 16819(73.60%), 16984(71.92%), and 16791(67.83%) respectively. On the other hand, variety 15743(66.67%) showed the lowest plant survival rate. The mean plant height ranges from 2.09m to 2.49m with an overall mean plant height of 2.31m. The tallest mean plant height (2.49m) was recorded in 15743 while shortest mean plan height (2.09m) was recorded in check. The mean leaf to stem ratio range from 1.71 to 1.28 with the overall mean of 1.52, and The higher leaf to stem ratio value (1.71) were recorded from check, followed by 16984(1.67), 15743(1.59), 16819(1.35) while the lowest value were recorded in 16791(1.28). In a combined analysis the mean dry matter yield range from 20.02 t/ha to 14.05 t/ha with the overall mean of 16.24 t/ha. The higher dry matter yield value (20.02 t/ha) were recorded from 16819, followed by 16791(16.77t/ha) and check (16.12t/ha) respectively. The lowest mean dry matter yield were recorded in 16984(14.05 t/ha) and 15743(14.26t/ha). Generally, Napier grass varieties tested has shown variation for agronomic performance and yield under rain fed condition in Gambella