A field experiment was conducted during the rainy (Kharif) seasons of 2013 to 2015 at Agriculture Research Station (Irrigation crops), Anand Agricultural University, Thasra.The farm is located in hot semi-arid eco-region with medium deep black soils and geographically situated at 22.400 latitude, 73.120 longitude and 32.4 m above the mean sea-level.To study the response of different levels of Nitrogen (N), Phosphorus (P) and Biofertilizers on Rice (Oryza sativa L.) under middle Gujarat Agro climatic zone III, 12 treatment combinations consisting three levels of Nitrogen, two levels of Phosphorus and two levels of Biofertilizer were tried in factorial randomized block design with four replications. The three-year experimental results revealed that different treatments for nitrogen levels were found significant for most of the growth and yield contributing characters, while for phosphorus and bio-fertilizer found non-significant. In pooled data the N level N3 (120 kg N ha-1) gave significantly higher grain yield of 5508 kg ha-1and was found significantly superior over N level of 80 kg (N1 ). The result revealed that the potential production and profit from the rice crop could be secured by applying120 kg Nitrogen ha-1 only to get higher yield in middle Gujarat Agro climatic zone III.