Agro-industrial Residues Research Articles

Structural and chemical characterization of lignin and hemicellulose isolated from corn fibers toward agroindustrial residue valorization

Structural and chemical characterization of lignin and hemicellulose isolated from corn fibers toward agroindustrial residue valorization

Assessing of the most appropriate biotechnological strategy on the recovery of antioxidants from beet wastes by applying the life cycle assessment (LCA) methodology

The valorization of agro-industrial waste streams and residues for the production of antioxidant compounds is a good strategy for circular economy approaches. However, to demonstrate its suitability and operational feasibility, it is necessary to develop environmental assessments to ensure the effectiveness of the production strategy. In this sense, a large-scale simulation has been developed, obtaining ten different scenarios in which both leaves and steam residues are used as process inputs, and five different extraction techniques are applied, both conventional: Soxhlet and maceration, and emerging technologies: ultrasonic assisted extraction (UAE), supercritical fluid extraction (SFE) and pressurized liquid extraction, (PLE). Environmental results have shown that SFE and PLE technologies have the lowest environmental burdens, while UAE has the worst profile due to high energy demand. Electricity could be considered as the main hotspot with the highest impact, followed by steam requirements and the use of extraction solvent. To improve the environmental profile, sensitivity analyses were performed, considering the use of renewable resources for the production of the energy requirements and the selection of the extraction solvent. Although significant improvements were obtained when electricity and steam production is based on hydropower and waste incineration, the environmental profile did not improve when considering ethanol: water mixture or hexane for extraction. Future research should focus on reducing energy requirements and optimizing the solvent dosage for the extraction process.

Pleurotus pulmonarius: a protease-producing white rot fungus in lignocellulosic residues.

The production of proteases by white rot fungi, such as those of the genus Pleurotus, is related to the degradation of wood proteins, the substrate on which these fungi grow in the environment. From the point of view of production, they are still little explored for this purpose. A selection of agro-industrial residues highlighted corn bagasse as the best substrate for solid-state protease production using the basidiomycete Pleurotus pulmonarius. The enzyme production was maximized through a factorial design, where the enzyme activity increased from 137.8 ± 1.9 to 234.1 ± 2.7 U/mL. Factors such as temperature stability, pH, and chemical reagents were evaluated. The optimum temperature was 45°C, showing low thermal stability at higher temperatures. The enzyme inhibition occurred by Mn2+ (50.3%) and Ba2+ (76.4%); SDS strongly inhibited the activity (82.4%), while pepstatin A partially inhibited (56%), suggesting an aspartic protease character. Regarding pH, the highest protease activity was obtained at pH 5.5. Partial characterization resulted in apparent values of the KM and Vmax constants of 0.61mg/mL and 1.79mM/min, respectively.

Finding a Needle in a Haystack: Producing Antimicrobial Cutin-Derived Oligomers from Tomato Pomace.

Agro-industrial residues comprise a rich diversity of plant polymers and bioactive compounds, constituting promising sources for the development of materials, including bioplastics, and food supplements, among other applications. In particular, the polyester cutin is abundant in fruit peel, a plentiful constituent of pomace agro-industrial residues. The potential of diverse fruit pomaces as a source for the development of cutin-derived materials/products has been extensively sought out. This study expands the established knowledge: it sets proof of concept for the production of antimicrobial oligomers from cutin-rich materials isolated in a single step from tomato pomaces generated by two remote agro-industries. Specifically, it first analyzed how the chemical signature (nuclear magnetic resonance (NMR) and gas chromatography–mass spectrometry (GC–MS)) of a pomace (and of its major constituents) mirrors that of the corresponding cutin-rich material isolated using an ionic liquid extractant. The cutin-rich materials were then deconstructed (using mild hydrolyses), and the resultant mixtures were chemically characterized and screened for bactericidal activity against Escherichia coli and Staphylococcus aureus. The presence of esterified structures, linear and/or branched, likely comprising dioic acids as a major building block (but not exclusively) is a prerequisite for activity against E. coli but not against S. aureus that was susceptible to monomers as well. Further studies are required to optimize the production of broad bactericidal oligomers from any cutin-rich pomace source, moving ahead toward their circular usage.

High-rate in-vessel continuous composting of olive mill byproducts

The increasing production of agro-industrial organic residues in modern society is extremely concerning. One of the most polluting procedures in the agricultural industry is the production of olive oil. This process creates a large amount of waste with high organic load and phytotoxic components. In this study, composting of two-phase olive pomace (OP), olive leaves (OL) and dewatered anaerobic sludge (DAS) from an olive mill wastewater anaerobic digestion process was conducted in a pilot-scale in-vessel high-rate continuous composter. Five different feed scenarios were studied with different OP/OL ratio in the feed material, while the effect of the addition of pine tree bark pieces (PB) and DAS was examined. The OP:OL 95:5 % w/w ratio exhibited the best results in terms of product quality, while OL proved capable of acting as a bulking agent for the better aeration of the material. The final product in the optimum feed ratio was free of Salmonella spp., was stable in terms of static respiratory index (lower than 0.5 g O2 kg−1 VS h−1) but contained elevated E. coli levels (3.5 × 104 CFU g-1 with a limit of 1 × 103 CFU g-1), which was the only EU proposed compost quality criteria not met. The addition of a more easily degradable material in the feed mixture is expected to lead to elevated composting temperature and amend the presence of pathogens.

Potential of New Bacterial Strains for a Multiproduct Bioprocess Application: A Case Study Using Isolates of Lactic Acid Bacteria from Pineapple Silage of Costa Rican Agro-Industrial Residues

Lactic acid bacteria (LAB) with potential for the development of multi-product processes are necessary for the valorization of side streams obtained during the biotechnological production of lactic acid (LA). In this study, 14 LAB strains isolated from pineapple agro-industrial residues in Costa Rica were cultivated in microplates, and the six strains with the highest growth were selected for fermentation in microbioreactors to evaluate the production of LA and acetic acid, and the consumption of glucose. Lacticaseibacillus paracasei 6710 and L. paracasei 6714 presented the highest OD600 values (1.600 and 1.602, respectively); however, the highest LA (in g/L) production was observed in L. paracasei 6714 (14.50 ± 0.20) and 6712 (14.67 ± 0.42). L. paracasei 6714 was selected for bioreactor fermentation and reached a maximum OD600 of 6.3062 ± 0.141, with a LA yield of 84.9% and a productivity of 1.06 g L−1 h−1 after 21 h of fermentation. Finally, lipoteichoic acid (LTA) detection from biomass was performed and the antimicrobial activity of the compounds present in the supernatant was studied. LTA was detected from L. paracasei 6714 biomass, and its supernatant caused significant inhibition of foodborne surrogate microorganisms. LAB isolated from pineapple silage have biotechnological potential for multiproduct processes.

Combined Bio-Hydrogen, Heat, and Power Production Based on Residual Biomass Gasification: Energy, Exergy, and Renewability Assessment of an Alternative Process Configuration

Bio-hydrogen from residual biomass may involve energy-intensive pre-treatments for drying and size management, as in the case of wet agro-industrial residues. This work assesses the performance of an alternative process layout for bio-hydrogen production from citrus peel gasification, with the aim of cogenerating heat and power along with hydrogen, using minimal external energy sources. The process consists of an air-steam fluidized bed reactor, a hydrogen separation unit, a hydrogen compression unit, and a combined heat and power unit fed by the off-gas of the separation unit. Process simulations were carried out to perform sensitivity analyses to understand the variation in bio-hydrogen production’s thermodynamic and environmental performance when the steam to biomass ratios (S/B) vary from 0 to 1.25 at 850 °C. In addition, energy and exergy efficiencies and the integrated renewability (IR) of bio-hydrogen production are evaluated. As main results, the analysis showed that the highest hydrogen yield is 40.1 kgH2 per mass of dry biomass at S/B = 1.25. Under these conditions, the exergy efficiency of the polygeneration system is 33%, the IR is 0.99, and the carbon footprint is −1.9 kgCO2-eq/kgH2. Negative carbon emissions and high values of the IR are observed due to the substitution of non-renewable resources operated by the cogenerated streams. The proposed system demonstrated for the first time the potential of bio-hydrogen production from citrus peel and the effects of steam flow variation on thermodynamic performance. Furthermore, the authors demonstrated how bio-hydrogen could be produced with minimal external energy input while cogenerating net heat and power by exploiting the off-gas in a cogeneration unit.

Investigation of new natural coagulant - cationic hemicellulose associated with cationic tannin - for coagulation/dissolved air flotation (C/DAF) in the treatment of industrial effluent

The use of plant-based coagulants (natural coagulants) in wastewater treatments has potential advantages over the inorganic coagulants used commercially. This study evaluated organic coagulants cationic hemicelluloses (CH) synthesized from peanut shell and associated with commercial cationic tannin (TSG) for use as the primary coagulation/flocculation treatment, followed by solid-liquid separation via sedimentation/flotation by dissolved air (DAF). The assay was carried out in a jar test on effluent from a multinational industry in the grain processing sector, located in the city of Uberlândia-MG. Coagulation diagrams were determined using the data spatial interpolation method of the Kringing regression model and the Tukey test was used to assess the difference in the results obtained. The optimum removal points of turbidity removal efficiency (TRE), greater than 98%, were achieved for the TSG/CH association with 200 mg L-1 (pH 10.72), 350 mg L-1 (pH 9.72), 500 mg L-1 (pH 9.56) in sedimentation. For the separation by DAF, the association of TSG/CH resulted in TRE values greater than 95% at dosages of 350 mg L-1 (pH 9.59) and 500 mg L-1 (pH 7.92). Furthermore, the results indicate that the associated use of TSG/CH, a coagulation aid, favored the coupling of the DAF bubble-particle, resulting in a smaller volume of sludge. In addition, CH expanded the action of TSG to the basic region.
 Keywords: agroindustry residue, natural organic coagulants, primary physical chemical treatment.

Optimization of Autohydrolysis of Olive Pomaces to Obtain Bioactive Oligosaccharides: The Effect of Cultivar and Fruit Ripening

The valorisation of agro-industrial residues presents a challenge in obtaining economically sustainable and environmentally friendly industrial processes. Olive pomace is a by-product generated in large quantities, from olive oil extraction. This residue mostly consists of lignocellulosic materials. The aim of this study was to evaluate the potential use of extracted olive pomaces (EOP) obtained from olives with different ripening indexes (RI) and from different cultivars (Cobrançosa; RI = 2.5; 3.3 and 4.7; and Galega Vulgar; RI = 1.8; 2.9 and 4.8), to produce bioactive oligosaccharides from hemicelluloses by autohydrolysis. The hydrothermal treatment conditions were optimized by Response Surface Methodology, following a central composite rotatable design (CCRD), as a function of temperature (T: 142–198 °C) and time (t: 48–132 min), corresponding to severity factor (SF) values from 3.2 to 4.9. For all pomace samples, soluble sugar production was described by concave surfaces as a function of temperature and time. Autohydrolysis with SF equal or higher than 4.0 produced higher sugar yields, with maximum values around 180 g glucose equivalent/kg EOP for SF of 4.7 (190 °C/120 min) or 4.9 (198 °C/90 min). These values were similar for both cultivars and were not dependent on the ripening stage of the olives. Maximum oligosaccharide (OS) yields of 98% were obtained by autohydrolysis with SF of 4.0. The increase in SF to 4.9 resulted in a decrease in OS yield to 86–92%, due to the release of monomeric sugars. The monosaccharides were mostly xylose (55.8–67.7% in Galega; 50.4–69.0% in Cobrançosa liquid phases), and glucose, galactose, arabinose and rhamnose, in smaller quantities. Therefore, the production of bioactive xylo-oligosaccharides (XOS) from olive pomaces mainly depends on the hydrothermal conditions used.

Metagenomes and Metagenome-Assembled Genomes from Microbiomes Metabolizing Thin Stillage from an Ethanol Biorefinery.

ABSTRACTHere, we report the metagenomes from five anaerobic bioreactors, operated under different conditions, that were fed carbohydrate-rich thin stillage from a corn starch ethanol plant. The putative functions of the abundant taxa identified here will inform future studies of microbial communities involved in valorizing this and other low-value agroindustrial residues.

Effect of Fungal Fermentation on Enhancement of Nutritional Value and Antioxidant Activity of Defatted Oilseed Meals.

Agro-industrial residues contain high nutritive value. Nowadays, various advanced researches have been done for the production of various value-added products, using these wastes as substrates in the fermentation media. Flaxseed, mustard, and rice bran meal, residues of oil industry, were used as substrates for fermentation. Submerged fermentation with soil-isolated fungal species of the genus Aspergillus sp. was done for oil production by using these substrates in the fermentation media. Effect of fermentation by the oleaginous species of Aspergillus on the nutritive value and functional properties of flaxseed, mustard, and rice bran meal has been discussed for the first time in the present study. After fermentation, the seed meals showed substantial increase in the protein and ash content. The fungal strains utilized the carbohydrate present in the seed meals for the production of highly nutritional metabolites, which decrease the sugar contents of the meals. The fungi also showed extracellular amylase and cellulase activities which helped to hydrolyze the carbohydrates present in these meals, to utilize them for their metabolism. The enhancement was also observed in terms of antioxidant activity of the meals. Increase in the total phenolic and flavonoid contents was observed after fermentation along with radical scavenging activity of 1,1-diphenyl-2-picrylhydrazyl and 2,2-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid reagents and ferric reduction potential. These effects of fermentation modify these cheap waste materials into nutrient dense substrates, which could be further used in the formulation of value-added products.

Evaluation of the antioxidant and antimicrobial activity of extracts obtained from four agro-industrial residues after different pretreatments

Agro-industrial wastes are not of interest to the industry that generated them; however, they can be used for the extraction of high value-added compounds. Compounds with antimicrobial and antioxidant activity can be recovered from residues generated by different industries. Jalisco is a large agri-food producer therefore resides generated by established or growing industries in the state were selected. However, the extraction of the compounds of interest from residues cannot be carried out directly, they must be subjected to different treatments that break the matrices to release the components of interest and increase the extraction yield. In this study, aqueous, enzymatic, chemical-enzymatic and ethanolic extractions were used to evaluate the antioxidant and antimicrobial capacity of the extracts. As residues, agave bagasse and brewers spent grain were evaluated, as well as blackberry and raspberry, both showing the first stages of spoilage. For the evaluation of antioxidant capacity ABTS and DPPH techniques were used. The antimicrobial capacity was evaluated by observing the inhibition halo caused by the extracts, and the inhibition percentage using different concentrations of the extracts, with three Gram-positive bacteria, three Gram-negative bacteria and three yeasts. The techniques used for the recovery of compounds with antioxidant and antimicrobial activity were adequate, and it was possible to observe differences between pretreatments for the different residues. It should be mentioned that inhibition was observed in the growth of Gram-positive and Gram-negative bacteria using low concentrations (10% of extracts).

Torrefaction of low-value agro-industrial wastes using macro-TGA with GC-TCD/FID analysis: Physicochemical characterization, kinetic investigation, and evolution of non-condensable gases

Two low-value agro-industrial residues, passion fruit peel waste (PF) and pineapple peel waste (PA), were considered in this study as feedstocks to produce solid biofuels by torrefaction. The experiments were conducted in a macro-TGA with GC-TCD/FID analysis to investigate their torrefaction characteristics. The GC-TCD/FID analysis allowed quantifying the non-condensable gases, including CO, CO2, CH4 and H2. The impacts of pretreatment with torrefaction on the physicochemical properties were investigated by using proximate analysis, ultimate analysis, and calorific values. The torrefaction of PF and PA was investigated at three different temperatures (200, 250, and 300 °C) and two residence times (15 and 60 min). The torrefaction pre-treatment led to higher levels of elemental carbon, fixed carbon, HHV and energy density than in the raw biomass. Experimental results showed that the most favorable torrefaction condition for producing a solid biofuel with a high energy density is a moderate temperature of 250 °C and 15 min of residence time. Two models were applied to the torrefaction kinetics of the two agro-industrial wastes: one with the one-step method and the other with the two-step method. The two-step reaction method was the most appropriate for describing the torrefaction behavior of the two agro-industrial wastes in all conditions studied. As determined by the two-step reaction method, the torrefaction activation energies ranged from 17.9 to 199.9 kJ mol−1 for PF and 5.3–84.0 kJ mol−1 for PA. Findings from this study are potentially valuable for the design of large-scale equipment for the torrefaction of PF and PA residues.

Metagenomes and Metagenome-Assembled Genomes from Microbial Communities Fermenting Ultrafiltered Milk Permeate.

ABSTRACTFermentative microbial communities can be utilized for the conversion of various agroindustrial residues into valuable chemicals. Here, we report 34 metagenomes from anaerobic bioreactors fed lactose-rich ultrafiltered milk permeate and 278 metagenome-assembled genomes (MAGs). These MAGs can inform future studies aimed at generating renewable chemicals from dairy and other agroindustrial residues.

Building a Bioethanol Market in Mexico

Objectives: This article aims to analyze the economic effects of bioethanol policies in Mexico, where there have been several attempts to introduce biofuels into the market but so far, no success. Methodology: Technically, an endogenous-price mathematical programming model is developed emphasizing the agricultural and fuel sectors, which are embedded in a multi-region, multi- product, spatial partial equilibrium model. Bioethanol can be produced both from a dedicated crop and from agroindustrial residues. Three policy alternatives are considered as well as a base case in which, as now, liquid fuels are all derived from fossil sources. The first alternative consists of subsidies to biofuel producers, the second of blending mandates and the third of both combined.Results: Results show some losses for fuel and agricultural consumers, that are not offset by both ethanol producer and environmental gains. Limitations and implications: The base year data should be more recent, but it is very difficult to gather all this amount of information for a more recent year. Like any partial equilibrium model, the analysis ignores the effects outside the markets included in the model. Originality and value: Model developed for this research, and it is the first economic study on bioethanol in Mexico that reaches such a detailed analysis. Conclusions: This research suggests that it is possible to develop a bioethanol market in Mexico, but some compensating redistribution among economic groups may be needed if these policies are to be seen as politically sustainable.

CFD Analysis of Co-firing of Coke and Biomass in a Parallel Flow Regenerative Lime Kiln

The lime industry is a highly energy intensive industry, with a huge presence worldwide. To reduce both production costs and pollutants emissions, some lime production plants are introducing more environmentally-friendly energy sources, such as local agro-industry residues. In this paper, a numerical tool is presented, which simulates a large-scale Parallel Flow Regenerative (PFR) kiln that currently uses coke as main fuel. The developed tool aims at investigating the combustion process under conditions of co-firing of coke and biomass and to assist the plant operators in the optimization of such operating conditions. To achieve this goal, a two-way coupling Euler–Lagrange approach is used to model the dynamics of the particulate phase and their interaction with the gas phase. Pyrolysis, volatiles oxidation and char oxidation are modelled by kinetics/diffusion-limited model (for heterogeneous reactions) and mixture fraction approach (for homogeneous reactions). Moreover, two methods are investigated for representing the limestone bed: a porous medium (PM) approach and a “solid blocks” (BM) tridimensional mesh. Comparison of the results for the case of 100% coke showed that the ideal “blocks” method is more accurate as it adequately simulates the scattering of fuel particles through the PFR kiln anchor, which is limited with the PM approach. Moreover, the temperature profile, maximum and minimum temperatures, as well as CO2 and O2 concentrations at outlet, are comprised in the expected range for this technology, according to available literature. Finally, the predicted results of a co-firing case with 60% biomass (in mass) were validated with measurements in an industrial facility, with production capacity of 440 calcium oxide tons per day. The results suggest that the model is fairly accurate to predict gas temperature, as well as O2 and NOX concentrations at the kiln outlet. Although some improvements are recommended to refine the CFD predictions, these promising results and the high computational efficiency laid the foundation for future modelling of co-firing of coke and biomass, as well as the modelling of the lime calcination process. It also paves the way for facilitating the reduction of pollutant emissions thus contributing to a more sustainable lime production.Graphical

IS MERCERIZATION THE ONLY FACTOR FOR (PARTIAL) POLYMORPHIC TRANSITION OF CELLULOSE I TO CELLULOSE II IN CELLULOSE NANOCRYSTALS?

"The present study aims to investigate the influence of factors such as the source of cellulose and cellulose extraction and acid hydrolysis conditions on the partial polymorphic transition of Cellulose I to the allomorphic form Cellulose II in cellulose nanocrystals (CNCs). CNCs were obtained from cellulose fibers extracted from four agro-industrial residues in Ethiopia: teff straw (TS), enset fiber (EF), sugarcane bagasse (SB) and coffee hull (CH). The cellulose fibers were extracted under chlorine-free extraction conditions, comprising alkaline pretreatment (with 17.5% (w/v) sodium hydroxide), then hydrolyzed with 64% (w/w) sulfuric acid to synthesize the CNCs. The as-obtained CNCs were characterized with X-ray diffraction (XRD), Attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy, thermogravimetric analysis (TGA), and transmission electron microscopy (TEM). The XRD results revealed the CNCs isolated from cellulose fibers of EF and SB contained Cellulose I and II allomorphs like their cellulose precursors, but no Cellulose II was found in CH-CNCs. Morphological and dimensional studies of the CNCs by TEM showed shorter needle-shaped nanoscale structures. Higher alkaline conditions, with 17.5% sodium hydroxide, might not necessarily contribute to the polymorphic transition in lignocellulosic materials with higher lignin content, as evidenced in CH. Generally, the formation of Cellulose I and II allomorphs in the as-obtained CNCs was dependent on the cellulose source and cellulose extraction conditions, and less influenced by sulfuric acid hydrolysis."

Removal of copper ions from alembic cachaça using agro-industrial residues as biosorbents

Cachaça is a typical Brazilian distilled beverage made from fermented sugarcane juice. The copper levels in alembic cachaça are of great concern among producers, and the removal of this contaminant is important for the quality of the product. The present study aimed to remove copper ions from alembic cachaça by adsorption. Sugarcane bagasse, okara, Moringa oleifera Lam., three different types of zeolites, and two types of commercial charcoal were tested as biosorbents. The heat-treated sugarcane bagasse removed 100% of the copper present in a cachaça sample, while other low-cost natural adsorbents had close to 50% removal as was observed for M. oleifera seeds and okara. All adsorbents had porous and fibrous structures, favorable to adsorption. A kinetic study showed that a pseudo-second-order model was appropriate, with equilibrium times of 15 h for heat-treated sugarcane bagasse, okara, and M. oleifera seeds used as adsorbents. The Langmuir model better fitted the experimental data, indicating monolayer adsorption. The maximum adsorption capacity was 17.00, 0.77, and 5.33 mg of Cu g−1 for the heat-treated sugarcane bagasse, M. oleifera seeds, and okara, respectively. The results presented here are promising indicating three agro-industrial residues were favorable to the adsorption of copper ions from alembic cachaça.

Development and Characterisation of Arabinoxylan-Based Composite Films

In the last decades, the overuse of synthetic polymers in the packaging industry has become a serious global environmental problem due to their nonbiodegradability. To overcome this issue, attention has been driven to study alternative materials, namely the use of biodegradable biopolymers extracted from agro-industrial residues, as materials for food packages. In this work, the polysaccharide arabinoxylan, previously extracted from corn fibre by alkaline hydrolysis, was used to produce composite and multilayer films. The composite films were produced by casting an oil-in-water emulsion with different quantities of oleic acid, while the multilayer films (beeswax-arabinoxylan-beeswax) were manufactured by submerging the arabinoxylan films in a beeswax solution. Both film types, along with a film composed only of arabinoxylan, were characterised in terms of their antioxidant activity, optical and mechanical properties, surface hydrophobicity, and barrier properties against water vapour (WVP), gases, and ultraviolet-visible (UV-vis) radiation. All the films developed were soluble in water. The multilayer films were more advantageous than the emulsion-based ones due to their enhanced barrier properties against water vapour (WVP = 0.58 × 10−11 mol/m∙s∙Pa), oxygen (with a permeability of 3.28 × 10−12 mol·m−1·s−1·Pa−1) and UV-vis radiation and higher values of water contact angle (92.43°), tensile stress (4.11 MPa), and Young’s modulus (15.96 MPa). The films developed, especially the multilayer ones, showed a good potential to produce flexible packages for low-water-content food products (e.g., several types of nuts).

Oxidative and storage stability in beef burgers from the use of bioactive compounds from the agro-industrial residues of passion fruit (Passiflora edulis)

This work aims to evaluate the effect of extracts obtained from passion fruit residues on the oxidative, microbiological, and physicochemical quality of beef burgers during storage. Four types of treatments were considered, including control (without addition of antioxidants), BHT (100 mg kg−1 of butylated hydroxytoluene), AE (1000 mg kg−1 of aqueous extract), and EE (500 mg kg−1 of ethanolic extract). The ethanolic and aqueous extracts showed a concentration of total phenolics of 39.94 ± 1.52 mg GAE.g−1 and 11.91 ± 0.39 mg GAE.g−1, respectively. Extracts have also shown antioxidant and antimicrobial in vitro activity. Under the conditions tested, AE and EE have prevented lipid oxidation in beef burgers similar to BHT. As well as inhibiting the growth of mesophilic and psychrophilic microorganisms. The discoloration process was minimized up to the 9th day of storage with just AE. Therefore, agro-industrial passion fruit residues are sources of natural additives, with antimicrobial and antioxidant properties, which can be used by the meat industry to improve the oxidative and microbiological stability of beef burgers during storage.