The occupational health and safety of agricultural workers is a topic that has a direct impact on the agricultural sector worldwide. For this reason, investigations into ergonomic factors are relevant to the health and safety of agricultural workers. In this study, nine variables of the physical–ergonomic dimension were analyzed to determine which factors represent occupational risks for agricultural workers in Mayo Valley, Mexico. A sample of 200 people was considered. The sample was separated by gender and divided into groups according to age. A closed-ended survey was developed and validated to assess physical ergonomics variables using a five-level Likert scale. Using Principal Component Analysis, it was found that there are physical ergonomic variables that affect male agricultural workers more than female workers (the risk of carrying heavy objects, PE3, and the risk of performing repetitive movements, PE4). It was also found that certain physical ergonomic variables are not perceived as hazardous by agricultural workers (the risk of using inappropriate materials, PE9). In addition, various research findings are discussed to determine the implications and recommendations for improving the occupational health and safety of agricultural workers in Mayo Valley, Mexico.