Agricultural Research Council Research Articles

Effect of genotype and harvest season on quality characteristics of Cyclopia subternata: Phenolic content and sensory profile

Cultivation of selected Cyclopia species, including C. subternata, is the only viable option to ensure that the current growth trajectory of the honeybush industry is maintained. Gaps in honeybush production knowledge still exist, including the optimum harvest season for production of good quality honeybush, as defined by the phenolic content of the leaves, which is related to bioactivity of extracts, as well as the sensory profile of the herbal tea. The aim of this study was to determine the optimal harvest season for achieving the maximum accumulation of phenolic compounds in the leaves of six genotypes (SGD2, SGD3, SGD6, STB1, SHL2, SKB3) under evaluation by the honeybush breeding programme of the Agricultural Research Council. The major phenolic compounds were quantified by HPLC-DAD. Plants were harvested in summer, autumn, winter and spring. The plant material was also processed to determine whether genotype and harvest season affect the sensory profile of honeybush tea as determined by descriptive sensory analysis. Both genotype and harvest season affected the phenolic content of the leaves and the sensory profile of the herbal tea. Overall, the summer harvest delivered the better product. SGD3 emerged as the genotype with the highest total phenolic content of the leaves (p < 0.05), predominantly due to higher accumulation of the benzophenone, 3-β-D-glucopyranosyl-4-O-β-D-glucopyranosyliriflophenone, and xanthones, mangiferin and isomangiferin. However, herbal tea from SGD3 also had a higher intensity of the negative aroma attribute, ‘hay/dried grass’, although not significantly different from SGD2, SHL2 and STB1 (p ≥ 0.05). Considering the positive aroma attributes, STB1 had either the lowest or lower intensities (p < 0.05) of ‘fynbos-floral’, ‘apricot jam’, ‘fruity-sweet’ and ‘fynbos-sweet’ than the other genotypes. By identifying summer as optimum harvest time, a foundation is laid for screening of more genotypes to expand the genetic pool and to determine the effect of cultivation practices on quality.

Assessment of genetic variation in Bapedi sheep using microsatellite markers

This study was conducted to assess genetic variation in Bapedi sheep using 14 microsatellite markers. Blood samples were collected from 174 unrelated Bapedi sheep on six farms in various districts of Limpopo and from the Agricultural Research Council Animal Production Institute (ARC-API) in Gauteng. Genotypes from other South African indigenous sheep, namely Zulu (N = 14), Damara (N = 11), Dorper (N = 8), and Namaqua (N = 11), were included to represent reference populations. The effective number of alleles averaged 5.6 for across the Bapedi flocks and was 4.9 for the reference breeds. Among the Bapedi flocks, the observed heterozygosity (Ho) ranged from 0.56 ± 0.05 to 0.69 ± 0.03 and expected heterozygosity (He) values were between 0.75 ± 0.04 and 0.88 ± 0.01. Thus, there is considerable genetic diversity within the Bapedi sheep populations. However, the fixation index was high, indicating the possibility of inbreeding becoming a problem for these flocks. A neighbour-joining tree was constructed from the estimates of Nei’s genetic distances among flocks. The presence of Bapedi sheep flocks on all of the main branches of the tree along with one of the reference breeds suggests the present-day Bapedi is not an entirely distinct breed and that there are genetic differences between flocks of these South African indigenous sheep. Sustainable breeding and conservation programmes are needed to control inbreeding and to foreclose possible genetic dilution of Bapedi sheep.&#x0D; Keywords: genetic diversity, germplasm conservation, inbreeding, indigenous sheep

Fall Armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae): A recent threat and future management strategy in Nepal

Abstract. Fall Armyworm (FAW), Spodoptera frugiperda, is an invasive pest of maize including other cultivated crops such as rice, wheat, and many other vegetables. This invasive species was initially noticed in North-America and later reported in many countries of Africa in early 2016. In South Asia, this has been reported for the first time in India followed by Srilanka in 2018 and Bangladesh, China, Taiwan and Nepal in 2019. It is polyphagous in nature and damage has been reported in more than 80 plant species. The loss has to lead up to 50-80% in maize in severe situation. FAW is a distant flyer and can fly more than 100 km in a night and spread well in crop fields. As this pest is already invasive in many states of India and the likelihood of entry and spreading in Nepal is higher because of the open border between the countries and flexible government quarantine policy. The great socio-economic loss has been forecasted once this pest has received invasive status in Nepal. Recently, this crop has been confirmed by Nepal Agricultural Research Council (NARC) in Gaidakot area of Nawalpur district, Nepal and has been noticed in almost all parts of Nepal such as Sidhuli, Chitwan, Nawalparasi, Tanahun and Rupendehi districts. However, this information has not been endorsed by Nepal Plant Protection Office (NPPO), an official invasive species endorsing organization in Nepal. This species is spreading rapidly in maize growing areas and significant losses have been reported in maize crop by the farmers. The regular scouting, surveillance, and monitoring can be suggested to evaluate the pest status in the crop field. Habitat manipulation with the deployment of deterring crops ‘push’ such as desmodium (Desmodium uncinatum) and pest-attracting crop ‘pull’ such as Napier (Pennisetum purpureum) and Sudan grass (Sorghum vulgare sudanense) is suggested in a ‘push-pull strategy’, the most popular and successful method of FAW management in the maize field. Field sanitation, and conserving pest’s natural enemies are other integrated approaches. However, in a commercial maize field, a group of ‘soft’ and selective chemicals have been suggested for immediate control of this pest. This review compiled the recent informations available on FAW and is useful for farmers, researchers and policy makers to draw a roadmap for the future FAW management in Nepal.

Assessment of the genetic diversity of sweetpotato germplasm collections for protein content

Sweetpotato [Ipomoea batatas (L.) Lam.] is a relatively cheap source of nutrients for humans in most developing countries, including those in sub-Saharan Africa. Breeding for essential quality traits is an important goal of sweetpotato breeding programs. This may include root protein content (RPC), since protein-energy malnutrition is a common problem worldwide. The objective of this study was to quantify the genetic diversity in RPC present among sweetpotato accessions maintained in the genebank of the Agricultural Research Council (ARC) of South Africa in order to characterize and select the most promising clones for breeding. Both, Dumas combustion and Near-infrared Spectroscopy (NIRS) were used to determine RPC for a diverse set of accessions. In addition, total root yield and dry matter content were determined and root flesh colour recorded. Data was subjected to standard analysis of variance. A total of 227 sweetpotato accessions (53 introduced accessions, 18 local cultivars, 24 local landraces and 132 ARC breeding lines) were screened for total RPC, and a large variation was observed. As measured by the Dumas combustion, RPC values varied from 2.65 to 6.45% on a dry weight basis (dwb). RPC estimated by NIRS ranged from 0.68 to 11.51%. The NIRS proved to be an efficient and quick tool that can be useful in sweetpotato breeding programs towards selecting for quality traits such as RPC. A total of 31 medium to high yielding accessions with high, intermediate and low RPC were selected for genetic analysis and breeding for enhanced nutrition. Some promising germplasm included the local informal market cultivar ‘Impilo’ (orange-fleshed) at 6.18%, and the breeding line ‘2012-29-4’, seed imported from Mozambique, at 6.45%. NIRS values for ‘Tanzania’ and ‘Maria Angola’ were promising. The study is one of only a few that provides protein data for a large set of sweetpotato accessions from various parts of the world and is useful for sweetpotato breeding programs. Future work can focus on the determination of the concentrations of individual amino acids.

Using satellite-based weather data as input to SWAT in a data poor catchment

Satellite-based weather estimates are spatially and temporally continuous data that seamlessly capture weather variability across the globe. A need was identified to assess the usefulness and reliability of satellite-based weather estimates in the lower Vaal River Catchment due to the scarcity of weather data. We explored the utility of Climate Forecast System Reanalysis (CFSR) data for streamflow simulation in an important agricultural catchment in South Africa, using the Soil and Water Assessment Tool (SWAT). First, we compared CFSR precipitation against rain gauge measurements at an annual time step. Two models were set up, one with conventional gauge weather data obtained from the South African Weather Services (SAWS) and the Agricultural Research Council (ARC), and the other with CFSR data. Calibration and validation of the models were performed using Sequential Uncertainty Fitting ver.2 (SUFI-2) algorithm of the SWAT Calibration Uncertainties Program (SWAT- CUP) at five stream gauge sites, with the data split between 2003 to 2008 and 2009 to 2013 for calibration and validation respectively. A low statistical agreement between CFSR precipitation and rain gauge data was obtained. The model with gauge data slightly outperformed CFSR model before calibration and during validation, whereas CFSR performed better mainly during calibration. Overall, both models achieved fair simulations of streamflow and long-term monthly streamflow regime at both the main catchment outlet and interior sub-catchments. While conventional gauge data remain the most accurate and reliable data source, CFSR can play a role as an alternative data input where there is inadequate gauge data.

Diversity of Native and Exotic Fruit Genetic Resources in Nepal

Diversity in fruit genetic resources in Nepal is contributed by wild, indigenous and exotic sources. This study was carried out to bring together the available fruit species and cultivars at various stations of Department of Agriculture (DoA), Nepal Agricultural Research Council (NARC), Agriculture and Forest University (AFU) and private farms until the Fiscal Year 2017/2018. Altogather there were 47 species of fruits from tropical zone of Terai (Tarahara, Janakpur, Sarlahi, Parwanipur and Khajura) to cold temperature zone of high hills (Marpha, Rajikot and Satbanj) across the country. Apple diversity was found at Horticulture Research Station, Rajikot, Jumla and has introduced 25 spur type cultivars. National Citrus Research Programme (NCRP), Dhankuta was citrus most diversity areas and has maintained 130 exotic and indigenous germplasms of citrus species followed by NCFD, Kirtipur. Mango diversity was noted at RARS, Tarahara (16 cultivars), RARS, Parwanipur (25 cultivars), Farm of DoA-Sarlahi (30 cultivars), Farms of DoA-Janakpur (18 cultivars), AFU-Rampur (17 cultivars). Some of the private nurseries like Everything Organic Nursery, Patlekhet, Kavre and international organization like Technology Demonstration Centre of ICIMOD, Godawari, Lalitpur were also found to be a diversity centre of many exotic and indigenous germplasms of fruit species. These indigenous fruit genetic resources were also used to develop varieties such as Sunkagati-1 and Sunkagati-2 and Tehrathum Local of acid lime, Khoku Local of mandarin orange, 'Malbhog' of banana which were notified by the National Seed Board, SQCC. The unique fruit genetic resources were ‘Pharping Local’ (Asian sand pear), ‘Sindhuli Junar’ (sweet orange), ‘Dhankuta Local’ and ‘Manakamana Local’ (mandarin), Local Malbhog (banana), Bhaktapure Lapsi (Nepalese hog plum) etc. which have superior traits than exotic fruits. Unique and wild fruit species were yellow, black and red raspberries (Rubus ellipticus, R. foliolosus and R. acuminatus respectively), bale (Aegle marmelos), pummelo (Citrus grandis), citron (Citrus medica), sweet lime (Citrus limettoides), butter tree or chiuri (Basia buttyacea), tamarind (Tamarindus indica), black plum (Syzygium cumini), wild apple (Mallus baccata), rough lemon (Citrus jambhiri), bayberry (Myrica esculanta), edimayal (Pyrus pashia), black and white ebony (Diospyrus malbarica), wild species of olive (Olea ferruginea and O. glandulifera), wild kiwifruit (Actinidia callosa) etc. Most of the diversity studies were based on phenotypic descriptions. We believe that the number of species and genotypes listed in this article would be increased if detail survey is further carried out. Way forward to utilize these valuable genetic resources has also been discussed in this manuscript.

PCR Based Genotyping of Lulu Cattle of Nepal for A1, A2 Type Beta-caseins

Lulu is an indigenous breed of cattle (Bos taurus) found in high altitude regions of western Nepal. Population of Lulu cattle has been declining due to introgression with other exotic breeds to increase milk productivity. Here we aimed at finding potential approach for conserving Lulu cattle and its assets by studying the milk contents and investigating which variant of beta-casein protein is present in this breed. Beta caseins are an abundant protein in cow milk with A1 and A2 being the most common genetic variants of this protein. Consumption of A1 type of milk has numerous health-related complications whereas A2 type of milk has numerous human health promoting factors. We used restriction fragment length polymorphism (RFLP) for determining the A1 and A2 variant of beta casein in Lulu cattle. For performing DNA extraction, we collected (n = 18) blood samples of Lulu from Mustang and (n=17) Nepal Agriculture research council farm. The amplified fragments in 3% agarose at 251bp and 213bp respectively confirmed the presence of both A1 and A2 gene in Lulu; however, A2 was of greater abundance. Our study indicated that Lulu has A2 variant of beta-casein predominantly. The gene frequency of A1A1 is 0, A1A2 is 0.06 and A2A2 is 0.94. We further found that the allele frequency of A1 and A2 is 0.03 and 0.97 respectively. We designed special primer for sequencing CSN2 genes since A2 type beta casein gene was predominantly seen on Lulu. The sequencing result further supports our RFLP result as most of our samples have “C” nucleotide SNP in amplified CSN2 gene sequence. The Chi-square value of the current study is 0.04 which supports Hardy-Weinberg equilibrium inferring that Lulu cattle are still in the pure state, where there is no genetic introgression with the exotic breed for the sake of improvement of productivity.

Investigating selective media for optimal isolation of Brucella spp. in South Africa.

Bovine brucellosis in South Africa is caused mainly by Brucella abortus biovar (bv.) 1 and less frequently by B. abortus bv. 2. Bacterial isolation is regarded as the gold standard for diagnosis of Brucella species; however, it is not very sensitive. The aim of this study was to determine the selective medium with optimum antibiotic composition that will allow the growth of Brucella species (spp.) while inhibiting moulds, yeast and most, if not all, Gram-negative contaminants in South Africa. In the controlled experiment, modified Agrifood Research and Technology Center of Aragon (CITA) medium (mCITA) seemed to be the optimum selective medium for isolation of Brucella spp. as compared with Farrell’s medium (FM) and modified Thayer Martin (mTM), while FM inhibited the growth of most fungal and bacterial contaminants. Mean comparison between the three media used to culture B. abortus resulted in lower mean difference ranging from 0 to 2.33. In case of Brucella ovis, high mean difference was obtained when comparing FM with mCITA (10.33) and mTM (12). However, the mean differences of 0.67 and 1.67 were obtained when comparing mCITA and mTM media used to, respectively, culture pasteurised and raw milk spiked with B. ovis. Further optimisation at the Agricultural Research Council – Onderstepoort Veterinary Research Institute resulted in a comparable performance between FM and mCITA; however, mCITA allowed optimal growth of the fastidious B. ovis, which is generally inhibited on FM. Generally, mCITA seemed to be the optimum selective medium for isolation of Brucella spp., while FM inhibits the growth of most fungal and bacterial contaminants. Thus, veterinary laboratories can use mCITA and/or FM but should take into consideration the detection of factious Brucella isolated in the country or region.

HYDROLOGIC RESPONSE MODELLING IN LUTANANDWA RIVER CATCHMENT, LIMPOPO, SOUTH AFRICA, USING SOIL WATER ASSESSMENT TOOL (SWAT) MODEL

Flow simulation is important in planning and design of engineering hydraulic structures. The purpose of this study is to predict flow in the Lutanandwa river catchment in the Luvuvhu river basin, Limpopo, South Africa. The input data needed for SWAT model set up included Digital Elevation Model (DEM), hydro meteorological data, land cover and soils data acquired from various institutions in South Africa. The DEM was processed from contours in the Department of Hydrology and Water Resources, University of Venda. Meteorological data was acquired from the South African Weather Service (SAWS). Observed daily stream flow data was obtained from the Department of Water Affairs and Sanitation. Land cover data was acquired from processed satellite imagery obtained from the department of Environmental Affairs and ground truthing carried out to verify the land cover information. Soils information was obtained from the Agricultural Research Council – Institute of Soil, Climate, and Water (ARC-ISCW). The soils data was obtained in the form of geo referenced land type map. Based on assessment of the data status, the period 2002 to 2014 was selected for modeling. The meteorological data processed for this period was prepared in the appropriate format for model set up. Soil water characteristic calculator was used to estimate the soil properties required as input to SWAT model. The land cover types in the study area was converted to corresponding SWAT land use types. The statistical parameters required as input to the weather generator in the SWAT model was calculated using the pcpSTAT programme which was used to compute the parameters using weather information. All input data was processed and prepared in the appropriate format for model set up. The processed input data was loaded into the Geographic Information Systems (GIS) interface of the SWAT model to enable model set up for flow simulation.

Seasonal Variations in Semen Parameters of Zulu Rams Preserved at 10°C for 72 H During Breeding and Non-Breeding Season

The study was conducted to evaluate the seasonal variations in semen parameters of Zulu rams preserved at 10°C for 72 h. The study site was the Agricultural Research Council (ARC) Irene, during the breeding season and the non-breeding season. Zulu rams (n = 6, age = 3 years and average weight = 35 kg ±2.29) were used in this study. Semen was collected with the aid of artificial vagina. Macroscopic (volume and pH), microscopic (sperm concentration, progressions, velocities and velocity ratios and morphology) semen parameters were evaluated immediately after semen collection. Data were analyzed using General Linear Model (GLM) in Minitab 17®. Semen volume was higher during breeding season (0.97±0.25 mL) than non-breeding season (0.72±0.5 mL). Total sperm motility was higher (92.01±1.40%) at 24h during breeding season than non-breeding season (88.69±1.40%), thereafter declined drastically in both seasons. However, non-breeding season yielded a high progressive motility (50.86±1.63%, 33.77±1.63% and 27.56±1.63%) after 24, 48 and 72 h than breeding season (21.32±1.63%, 11.89±1.63% and 10.29±1.63%). It was concluded that, semen parameters from Zulu rams does vary with seasons. Nevertheless, despite seasonal variations observed, Zulu rams’ semen quality is acceptable in both breeding and non-breeding season hence can breed throughout the year. Notwithstanding, studies involving fertility are recommended and the nutrition effect of lambing during dry period should be considered.

Advances in fruit breeding in Nepal

Nepal has spent about six decades on fruit development and research in different species. Fruit breeding particularly local and exotic germplasm collection started after 1950s and has gained momentum after the formation of commodity programme in 1972 AD. Major researches in the past were focused on indigenous and exotic genotype collection, evaluation, selection, propagation protocol standardization and adoptive trials. Some good ground works have already been done in major fruits such as apple, pear, plum, persimmon, kiwifruit, citrus, litchi, guava, pomegranate, walnut, papaya, banana and mango. The major public institutions involved in fruit breeding are Nepal Agricultural Research Council (NARC) following Agriculture and Forestry University. Some of the private nurseries like Everything Organic Nursery, Kavre and Technology Demonstration Centre of ICIMOD, Lalitpur were also involved in introduction and maintenance of indigenous and exotic fruit species. National Centre for Fruit Development, Kirtipur; Tropical Region Horticulture Centre, Nawalpur; Temperate Horticulture Farm, Satbanj, and Horticulture Farm, Marpha collected many local and exotic fruits and maintained at field gene bank. Horticulture Research Station, Rajikot has introduced 25 spur type apple cultivars and maintained in field gene bank. National Citrus Research Programme, Paripatle has introduced, collected and maintained 130 genotypes including exotic and indigenous landraces of citrus. Two varieties of acid lime ‘Sunkagati-1’ and ‘Sunkagati-2’ have been released and one variety ‘Terhathum Local’ has been registered. ‘Khoku Selection’ of mandarin orange has also been registered. Banana varieties 'Malbhog', 'Willium Hybrid' and 'G9' has been selected by participatory varietal selection and registered. To strengthen fruit breeding in the nation, NARC needs to be restructured with special focus on fruit researches. Establishment of national fruit commodity programs along with establishment of Tropical Fruit Research Station in Province 2 and Temperate Fruit Research Station in Province 5 at national level can streamline NARC's fruit breeding researches.

Development of an equation to predict net protein requirements for the growth of Zebu beef cattle

The accurate estimation of protein requirements for beef cattle is a key factor in increasing livestock profitability and decreasing the environmental impacts of excessive N excretion due to mismatching between assumed requirements and diet formulation. A meta-analysis was conducted to evaluate and validate a new equation to predict the net protein requirements for growth (NPg) of Zebu beef cattle. For the development of the new approach, a database of 552 observations comprised of bulls, steers, and heifers of different genetic groups (Zebu, beef crossbreed, and dairy crossbreed) was assembled. The new approach was evaluated and compared to current models devised by the international nutrient requirements system committees (Agricultural Research Council, 1980; Beef Cattle Nutrient Requirements Model, 2016; BR-CORTE, 2016) to predict NPg. The model evaluation was performed through the model evaluation system (version 3.1.16) using an independent data set (n = 177 observations). An equation was considered the best estimator of NPg if the following conditions were met: (1) the intercept and slope of the regression between ordinary residues and/or predicted NPg values must have been equal to zero and one, respectively; and (2) the greatest concordance correlation coefficient (CCC) and determination coefficient (R), and lowest mean squared error of prediction (MSEP) were attained. Based on the regression models of the observed v. predicted NPg of Zebu beef cattle, both the new approach and that of the ARC (1980) correctly estimated NPg, since the intercept and slope were not different (P > 0.05) from zero and one, respectively. Additionally, the new approach’s determination coefficient was the greatest and the closest to one. The fact that the new model achieved a higher CCC and lower MSEP than the existing models indicated its superior reproducibility and accuracy. The equations proposed by BR-CORTE (2016) and the BCNRM (2016) did not correctly estimate NPg in that the intercept and slope were different (P < 0.01) from zero and one, respectively. Thus, the equations proposed by the new approach and the ARC (1980) accurately and precisely estimated NPg and are recommended for Zebu cattle. Furthermore, the inclusion of equivalent empty BW (EQEBW) in the new approach improves the estimation of NPg. We suggest the use of the following equation to calculate NPg for Zebu beef cattle: NPg = 176.01 × EBG – 0.381 × EQEBW0.75 × EBG1.035 (R = 0.80 and CCC = 0.75); where NPg = net protein requirements for growth, EBG = empty body gain, and EQEBW = equivalent empty BW.

Long-Chain Monounsaturated Fatty Acids Improve Endothelial Function with&amp;nbsp;Altering Microbial Flora

Background: Fish oil-derived long-chain monounsaturated fatty acids (LCMUFAs) with a carbon chain length longer than 18 units ameliorate cardiovascular risk factors in mice. In this study, we investigated whether LCMUFAs could improve endothelial functions in mice and humans. Methods: In a double-blind randomised placebo-controlled, parallel-group, multi-centre study, healthy subjects were randomly assigned to either an LCMUFA oil (saury oil) or a control oil (olive and tuna oils) group. Sixty subjects were enrolled and administrated each oil for 4 weeks. For the animal study, Apo-E−/− mice were fed a Western diet supplemented with 3% of either C20:1 or C22:1 fatty acids for 12 weeks. Findings: Participants from the LCMUFA group showed improvements in endothelial function, an increased GLP-1 level, and lower trimethylamine-N-oxide level, which is a predictor of coronary artery disease. C20:1 and C22:1 oils significantly improved atherosclerotic lesions and several plasma inflammatory cytokine levels including IL-6 and TNF-α. These beneficial effects were consistent with an improvement in the gut microbiota environment, as evident from the altered ratio of Firmicutes/Bacteroides, increase in the abundance of Akkermansia, and upregulation of short-chain fatty acid (SCFA)-induced glucagon-like peptide-1 (GLP-1) expression. Interpretation: These data suggest that LCMUFAs alter the microbiota environment that stimulates the production of SCFAs, which results in the induction of GLP-1 secretion. Fish oil-derived long-chain monounsaturated fatty acids might thus help to protect against cardiovascular disease. Trial Registration: This trial was registered at UMIN Clinical Trials Registry (registration number: UMI29181). Funding Statement: This study was supported by JSPS KAKENHI, grant number 19H04055, and the Agriculture, Forestry and Fisheries Research Council, grant 27016A. Declaration of Interests: The authors have declared that no conflict of interest exists. Ethics Approval Statement: This trial was approved by the Committee for Medical Ethics at Tokushima University Hospital, Tokushima, Japan (approval number: 3047). Written informed consent based on the Declaration of Helsinki was obtained from all participants.

Prospects and Challenges of Sugarcane Development in Nepal: Production, Market and Policy

Sugarcane is a major cash crop and contributes 2.1% in AGDP of Nepal. This paper attempts to review and analyze the status and issues of sugarcane subsector in reference with production, market and policies. Nepalese sugarcane production contributes less than 1% among SAARC countries and ranks 41st position among global sugarcane production status. Per capita consumption of sugar in Nepal is 8 kg/year and the annual production of the sugar contributes 60% of total national demand. Average recovery percentage of sugar in Nepal is very less (8.83%) as compared to other major producer countries. Very limited research activities on varietal development and technology enhancement in sugarcane has been done by Nepal Agricultural Research Council. Policy intervention of government to promote sugarcane are mainly limited to cash subsidy to the farmers, simple technology extension services, fixation of minimum procurement price and farm facilitation. There is an absence of dedicated sugarcane development organization in present context. Delay payment to the farmers, traditional technologies, lack of high yielding varieties and poor access of farmers to the agriculture loan and insurance are found as major challenges of sugarcane production in Nepal. The study suggested to establish dedicated organization to promote the sugarcane technology extension and policy facilitation from the government. Establishment of quick and transparent payment mechanism to the farmers is must to encourage farmer for sugarcane cultivation in Nepal.

Effects of different fertilizers on the growth and yield of okra (Abelmoschus esculentus L.) in summer season in Chitwan, Nepal

An experiment was conducted in the Horticulture Farm of Agriculture and Forestry University to demonstrate the effects of different fertilizers on the yield and yield parameters of okra (var. Arka Anamika). The experiment was laid out in Randomized Complete Block Design (RCBD) consisting of seven treatments and three replications. The various treatments used in the experiment were goat manure, sesame cake, mustard cake, synthetic fertilizer (NPK), poultry manure, vermicompost and untreated control. The required dose of nitrogen was fulfilled by the fertilizer itself whereas insufficient amount of phosphorous and potassium was fulfilled by addition of single super phosphate and muriate of potash respectively. The fertilizers were applied on the basis of recommendation given by the Nepal Agriculture Research Council (NARC). The effect of poultry manure on number of open flowers and number of fruits at 40 DAS was found superior. The effect of poultry manure on plant height, number of leaves, plant diameter was found superior at 50 DAS. Goat manure produced the superior result on number of open flowers at 50 DAS. Poultry manure on the number of leaves and plant height produced the significant result at 60 DAS. Synthetic fertilizer responded well to number of fruits at 60 DAS. Sesame cake produced the superior results at 70 DAS on number of buds. Poultry manure responded well to all the parameters and produced the yield of 200 qt./ha with the BC ratio of 1.77. This experiment suggests the farmers to use the poultry manure to get the highest economic return. Vermicompost and mustard cake producing the superior and significant yield in this research are not recommended as they have low BC ratio unless effective measures are encouraged to reduce the cost of this fertilizers.

Short-term association between ambient temperature and homicide in South Africa: a case-crossover study

BackgroundCriminology research has traditionally investigated sociodemographic predictors of crime, such as sex, race, age, and socioeconomic status. However, evidence suggests that short-term fluctuations in crime often vary more than long-term trends, which sociodemographic factors cannot explain. This has redirected researchers to explore how environmental factors, such as meteorological variables, influence criminal behavior. In this study we investigate the association between daily ambient temperature and homicide incidence in South Africa, a country with one of the highest homicide rates in the world.MethodsMortality data was from South Africa’s civil registration system and includes all recorded deaths in the country from 1997 to 2013 (17 years). Daily temperature was from the National Oceanographic and Atmospheric Association of the United States and South Africa’s Agricultural Research Council. Data were analyzed using a time-stratified case-crossover design with conditional logistic regression. We delineated cases as either “definite” (ICD-10 codes X85-Y09, n = 68,356) or “probable” homicides (ICD-10 codes W25-W26, W32-W34, W50, Y22-Y24, Y28-Y29, n = 177,873). Case periods were defined as the day on which a death occurred. Control periods were selected using a day-of-week match within the same month and district. Analyses investigated same-day and lagged effects of maximum, mean and minimum temperature.ResultsA one-degree Celsius increase in same-day maximum temperature – our a priori metric of choice – was associated with a 1.5% (1.3–1.8%) increase in definite homicides and a 1.2% (1.1–1.3%) increase in total (definite + probable) homicides. Significant (p < 0.05) positive associations were also observed when applying other temperature metrics (mean, minimum) and lags (1, 0–1). The shape of the association did not display any clear non-linearities. There was no evidence of confounding by public holidays or air pollution.ConclusionsThis study suggests a positive association between daily ambient temperature and homicide in South Africa. This temperature-health relationship may be of particular concern in the context of climate change. The ability to include meteorological variables as a predictor of criminal activity and violent behavior could prove valuable in resource allocation for crime prevention efforts.

Participatory on-farm evaluation of wheat genotypes

In wheat development programs, the evaluation and identification of superior genotypes is the first and leading step in a crop improvement program. Coordinated Farmer’s Field Trial (CFFT) was conducted during the three successive wheat growing season of 2010/11, 2011/12 and 2012/13. In CFFT six different wheat genotypes were planted in different outreach sites of research stations of Nepal Agricultural Research Council (NARC) at varying geographical regions. CFFT was conducted according to standard recommended practices of wheat at farmers’ field with different sets of genotypes for Terai and hill. In CFFT for Terai Tar and Lower valley (TTL) under timely sown irrigated (TSI) condition wheat genotype NL 1073 produced the grain yield of 3695 kg/ha and under the timely sown rainfed (TSR) that was 2738 kg/ha in 2010/11. In 2011/12, wheat genotype NL 1073 had the highest recorded grain yield of 3691 kg/ha in mid western region which was followed by check variety Vijay in CFFT-TTL in 2011/12 in the same region. Similarly in 2012/13, check variety Vijay showed the highest grain yield of 3818 kg/ha and 3044 kg/ha followed by NL 1094 (2938 kg/ha and 3468 kg/ha) in TSR and TSI environments, respectively. In CFFT for Mid and High Hill (MHH) WK 1204 had the highest grain yield of 3967 kg/ha in TSI which was followed by NL 1008 with the yield of 3890 in 2010/11. In 2011/12 the highest mean grain yield was observed in WK 1204 (4242 kg/ha) followed by BL 3872 (3922 kg/ha). Similarly, in 2012/13 NL 1008 was the best genotypes on the basis of grain yield (3297 kg/ha) followed by NL 1055 (3131 kg/ha) under CFFT-MHH.

Host-status of 20 sweet potato lines to Meloidogyne species in South Africa

ABSTRACTExperimental system: Due to serious economic challenges posed by root-knot (Meloidogyne species) nematodes in sweet potato (Ipomoea batatas) production, the Sweet Potato Programme (SPP) of the Agricultural Research Council (ARC) in South Africa has since included screening for nematode host-status in its breeding-selection activities. Procedures: 20 sweet potato lines were screened against M. javanica, M. incognita race 2 and M. incognita race 4 in parallel trials inoculated with 3000 eggs and second-stage juveniles (J2) per established cutting. Results: At 56 days after inoculation, the reproductive potential (RP) of all test Meloidogyne species on sweet potato line 1990-10-2 was zero, whereas RP values on other lines were 19.48–342.7, 31.9–995.1 and 10.3–380.44 ranges for M. javanica, M. incognita race 2 and M. incognita race 4, respectively. Conclusion: Among the test sweet potato lines, line 1990-10-2 was non-host to populations of tropical Meloidogyne species in South Africa and could, therefore, be subjected to nematode resistance tests.

Returns to Potato Research Investment in Nepal

Agricultural research plays a significant role in increment in productivity and contributes to food security. Very few studies on the impact of public research spending on different sectors and commodities in Nepal has been documented. This study was designed to estimate the adoption lags of improved potato varieties and benefits generated by potato improvement research in Nepal. More specifically, the study answers two basic questions: (i) whether investment in potato research is justifiable? and (ii) how long is it taking to replace old improved varieties? We took public annual potato research investments of Nepal from the Fiscal year 2001 to 2017 from Nepal Agricultural Research Council (NARC). The household survey was carried out to estimate the potato varieties coverage in Nepal in 2017. Other secondary data were sourced from the Ministry of Finance, Ministry of Agricultural and Livestock Development, FAO stat, Nepal Rastra Bank and NARC to complement the analysis. We have used a simple economic framework to estimate the benefits generated from agricultural research. The estimated benefit and cost streams were used to calculate Benefit-Cost Ratio (BCR) and Net Present Value (NPV). Results showed that the majority of the potato researchers had done research related to crop husbandry (seed production and crop managements); while the limited number of researchers had specialized in breeding activities. The annual growth rate of potato productivity was 214.49 kg per annum per ha which had increased at the rate of 1.76 percent per annum compound growth rate. The results revealed that the average weighted age of improved potato varieties was 21.83 years and each NPR. investment on potato research has given 508 NPR. and NPR. 13760 million NPV at a 12 percent discount factor during the last 17 years. Similarly, the contribution of potato research to GDP and AGDP was found 0.323% and 0.989% respectively. Therefore, future investment and focus should be concentrated on potato research and scaling up activities, and reducing variety adoption lag to obtain additional benefits from potato research investment.

Towards the development of a heat-health early warning system for South Africa

TPS 664: Climate change: temperature effects 2, Exhibition Hall, Ground floor, August 27, 2019, 3:00 PM - 4:30 PM Climate change has been associated with changes in temperature extremes globally. This includes a higher frequency and severity of hot days and heatwaves. Exposure to high temperatures poses a significant public health concern as people of all ages are at risk of the negative health effects of exposure to heat. These include cramps, fainting, heat exhaustion, heat stroke, dehydration, disease exacerbation and death in some cases. Heat alerts in South Africa are currently based on defined temperature-fixed threshold values for large towns and cities. However, heat-health early warning systems (HEWS) should be based on thresholds that are related to heat-related health outcomes. Therefore, this study aims to contribute towards the development of an evidence-based HEWS for South Africa that incorporates high temperatures and mortality. Mortality data were provided by Statistics South Africa for 1997 to 2013. Daily minimum, maximum and mean temperatures were obtained from the National Oceanographic and Atmospheric Administration and South Africa’s Agricultural Research Council. The joint CCl/CLIVAR/JCOMM Expert Team on Climate Change Detection and Indices has developed a core set of 27 temperature extreme indices that is widely used as a tool to assess and monitor changes in weather and climate extremes. RClimDex developed by Zhang and Feng at the Climate Research Branch of Meteorological Service of Canada was used to calculate monthly extreme temperature indices for each district (n = 52). The random forest (RF) algorithm for regression was used to assess the effect of the calculated indices on mortality by identifying the most significant index in each district using the variable importance score. Sensitivity analysis included tuning parameters within RF. Diurnal temperature range was the index most frequently selected as a top variable. Results are discussed in light of recommendations for HEWS for South Africa with relevance to other southern African and developing countries.