Agricultural Drainage Research Articles

Understanding the Effect of Seasonal Climate Variability on the Salinity in Unsaturated Agricultural Soil

Salinization/desalinization processes in the soil vadose zone are important to define agricultural irrigation and drainage schedules, especially in reclaimed crop areas. Numerical modeling of soil–climate interaction is a very helpful tool to understand soil salinity distribution and solute transport and therefore define efficient desalination solutions. A finite element analysis program Code_Bright was used to perform a coupled thermo-chemo-hydraulic analysis aiming at investigating the effect of climate actions on the distribution of soil salinity in depth, by modeling solute transport in the vadose zone under fresh/saline groundwater supply. The analysis separated first the effect of rain infiltration and evaporation, and then a real climate was considered as the boundary condition. A downward flow pattern induced by rainfall in the unsaturated zone resulted in a nonlinear salt leaching process. Significant differences in salt concentration between the surface and lower layer caused by rainfall resulted in a decrement in the leaching efficiency. Evaporation causes water to move upward and salt transport to the surface, thus enhancing the soil salinity above the evaporation front. The salinity above the groundwater table and below the evaporation front were less affected regardless of the salinity of the supplied groundwater. The model simulated the salt leaching process during the wet seasons and salt accumulation processes during the dry ones. The soil salinity and saturation at the soil surface have significantly responded to seasonal climate variability. A typical seasonal climate variability would result in a low salt leaching efficiency through years in the coastal reclamation area. These results would be helpful for the design of soil salinization management strategies, such as reducing salt accumulation by reducing evaporation or leaching the surface salt in the dry season, and increasing the drainage to promote leaching in the wet season.

Advantages of using a carbon-rich substrate in a constructed wetland for agricultural water treatment: Carbon availability and biota development

Proper bed substrate selection is essential for the growth of microorganisms and plants, which are key elements in constructed wetlands (CWs) performance. This is even more important in CWs that treat irrigated agricultural drainage water characterised by a high nitrogen (N), but low carbon (C) and phosphorus (P) concentration. This nutrient imbalance compromises biota activity and limits water treatment processes. Three substrates were tested in a CW field-scale pilot plant for 2 years: gravel (100%) and two mixed substrates, gravel + 30% natural wetland soil and gravel + 10% biochar. While gravel is one of the most commonly used substrates in subsurface flow CWs, soil has hardly been employed, and field-scale biochar application studies are scarce. We analysed the effect of adding a C-rich substrate to gravel on both nutrient imbalance correction and the biotic performance of CWs. Adding natural soil or biochar increased P availability in beds. However, the dissolved organic C concentration in interstitial water was only enhanced by soil addition. Unlike gravel, microbial density and activity, and Phragmites australis plant growth, were higher in beds with soil, followed by those with biochar. A similar pattern was observed for plant tissue quality. Although biochar proved positive for biota, it had a short-term effect as a source of C and P. Therefore, employing a suitable gravel-soil mixture provides notable advantages for the irrigated agricultural water treatment challenge. Even after considering some limitations, our results represent an important step to design CWs that treat this wastewater type and for eutrophication control in sustainable agroecosystems.

Spatiotemporal patterns and main driving factors of drainage water quality of an arid irrigation district with shallow groundwater table.

Drainage water quality is a crucial factor reflecting the regime of agricultural non-point source pollution in irrigation districts and is closely related to land use, soil texture, cropping pattern, fertilization, and irrigation and drainage conditions. However, the response of drainage water quality to various natural and anthropogenic factors needs further exploration in irrigation districts affected by shallow groundwater table. Spatiotemporal patterns of chemical oxygen demand (COD), total phosphorus (TP), total nitrogen (TN), and ammonium nitrogen (NH4-N) were monitored and analyzed in ten agricultural drainage ditches in the arid region of China from 2011 to 2019. Spatially, water pollution in agricultural drainage ditches with small water quantity can be significantly exacerbated by urban sewage, whereas a large amount of agricultural drainage can effectively dilute the pollution of urban sewage. Severe soil salinization in the cropland increases the risk of water pollution due to easier losses of soil nutrient and organic matter. Soil salinization is a key factor in the crop distribution pattern based on the crop salt tolerance, and the maize/wheat field with a higher fertilizer application rate generally results in poorer drainage water quality. Temporally, for the agricultural drainage ditches, the monthly and annual COD, TP, TN, and NH4-N concentrations fluctuate inversely with drainage water quantity and are positively correlated with fertilizer application, among which the monthly COD concentration in drainage water has larger variation in severe salinized areas. There exist critical annual and monthly drainage amounts, above which the probabilities of higher concentrations of COD, TP, TN, and NH4-N reduce greatly.

Spatio-temporal distribution and source identification of antibiotics in suspended matter in the Fen River Basin

In this study, 26 typical antibiotics in the suspended matter of the Fen River basin were analyzed during the wet and dry seasons, and the main sources of antibiotic contamination were further identified. The results showed that the concentrations of antibiotics in the suspended matter varied seasonally. Sixteen antibiotics were detected in the suspended matter during the wet season with an average concentration of 463.56 ng/L. However, a total of 21 antibiotics were detected in the dry season, with an average concentration of 106.00 ng/L. The concentration of chloramphenicol antibiotics was outstanding in the wet season and dry season. The spatial distribution of the antibiotics in suspended matter showed little spatial discrepancy during the wet season. During the dry season, nevertheless, the concentration was higher upstream than midstream and downstream. The main sources of antibiotics in the Fen River Basin were livestock and poultry breeding, wastewater from wastewater treatment plants (WWTPs), agricultural drainage, domestic sewage, and pharmaceutical wastewater. Wastewater from WWTPs and domestic sewage were identified as two primary sources in the suspended matter during the wet season, with wastewater from WWTPs contributing the most accounting for 37%. While the most significant source of antibiotics in the suspended matter in the dry season was pharmaceutical wastewater, accounting for 36%. In addition, the contribution proportion of sources for antibiotics exhibited discrepant spatial distribution characteristics. In the wet season, wastewater from WWTPs dominated in the upstream and midstream, and livestock and poultry breeding was prominent in the midstream and downstream. Pharmaceutical wastewater was the main source in the midstream and downstream regions during the dry season.

Addressing the C/N imbalance in the treatment of irrigated agricultural water by using a hybrid constructed wetland at field-scale

To mitigate excess of nitrate-N (NO3−-N) derived from agricultural activity, constructed wetlands (CWs) are created to simulate natural removal mechanisms. Irrigated agricultural drainage water is commonly characterized by an organic carbon/nitrogen (C/N) imbalance, thus, C limitation constrains heterotrophic denitrification, the main biotic process implicated in NO3−-N removal in wetlands. We studied a pilot plant with three series (169 m2) of hybrid CWs over the first two years of functioning to examine: i) the effect of adding different C-rich substrates (natural soil vs. biochar) to gravel on NO3−-N removal in a subsurface flow (Phase I), ii) the role of a second phase with a horizontal surface flow (Phase II) as a source of dissolved organic C (DOC), and its effect in a consecutive horizontal subsurface flow (Phase III) on NO3−-N removal, and iii) the contribution of each phase to global NO3−-N removal. Our results showed that the addition of a C-rich substrate to gravel had a positive effect on NO3−-N removal in Phase I, with mean efficiencies of 40% and 17% for soil and biochar addition, respectively, compared to only gravel (0.75%). In Phase II, the algae growth turned into a DOC concentration increase, but it did not enhance NO3−-N removal in Phase III. In series with C-rich substrate addition, the largest contribution to NO3−-N removal was found in Phase I. However, in series with only gravel, Phase II was the most effective on NO3−-N removal. Contribution of Phase III to NO3−-N removal was almost negligible.

Timing of reed management affects habitat use and breeding success in Great Reed Warblers: A field experiment on agricultural drainage canals

Freshwater wetlands and their biodiversity have suffered substantial losses globally, mainly due to the drainage of wetlands for agriculture. Recent studies suggest that small drainage and/or irrigation canals established in lowland areas can function both as partial replacement of wetlands lost and as biodiversity hotspots in agricultural landscapes. However, small canals are usually not protected or managed for biodiversity. We studied whether and how the timing of reed management (removal in winter, in spring, no removal) on small canals affects nest site selection and breeding success in Great Reed Warblers, Acrocephalus arundinaceus, a flagship species of small canals in an intensive agricultural landscape in a five-year quasi-experiment. We found significantly more nests in winter-managed than in non-managed canal sections and hardly any in spring-managed sections. The number of nests also increased with the proportion of reed removed and with reed density. Breeding success measured as the probability of fledging chicks was positively influenced by reed density only and was not affected by management type, nesting height, water depth and water level fluctuation. Reed density had a central role as it mediated the effect of management both on nest site selection and breeding success, likely through better concealment of the nests from predators. Our results suggest that winter management should be favored as much as possible in the conservation of Great Reed Warblers and other reed-nesting passerines, whereas spring management should be avoided as it results in a complete lack of reed or poorly grown reed unsuitable for nest-building by the start of the breeding season. Our study draws attention to the importance of proper management of small canals that can serve as local hotspots of biodiversity in heavily human-modified landscapes, and which thus deserve more conservation attention than they currently have.

Impacts of biostimulation and bioaugmentation on woodchip bioreactor microbiomes.

Nitrate pollution is a serious problem in agricultural areas in the U.S. Midwest and other parts of the world. Woodchip bioreactor is a promising technology that uses microbial denitrification to remove nitrate from agricultural subsurface drainage, although the reactor's nitrate removal performance is limited under cold conditions. This study showed that the inoculation of cold-adapted denitrifiers (i.e., bioaugmentation) and the addition of labile carbon (i.e., biostimulation) can influence the microbial populations and enhance the reactor's performance under cold conditions. This finding will help establish a strategy to mitigate nitrate pollution.

Investigating Microbial Triggers of Nitrous Oxide Emissions in Agriculturally Influenced Aquatic Ecosystems

The agricultural sector in Canada is responsible for approximately 9% of greenhouse gas (GHG) emissions in Canada, accounting for 54 MT of carbon dioxide (CO2), as well as 31% of methane (CH4) and 75% of national nitrous oxide (N2O) emissions in 2021 (Environment and Climate Change Canada 2021). However, these estimates do not include the indirect GHG emissions that occur in agriculturally impacted waterways. Ontario is home to over 45 000 kilometers of agricultural drainage ditches, with tile drains directly connecting terrestrial and aquatic environments. Microbial biogeochemical cycles in the causeways experience fluxes of nutrients leading to hotspots for GHG at the sediment-water interfaces. Along with fluxes of nutrients, the causeways are regularly disturbed by anthropogenic effects (e.g., dredging, removal of vegetative buffers) and increasing frequency of storm events. Previous studies have used static chambers to evaluate GHG emissions from aquatic systems (Mu et al. 2022, Xiao et al. 2016). However, this approach can be time consuming and labour intensive and is impractical in aquatic systems due potential extensive underestimation of fluxes from diffusion. To resolve driving factors contributing to GHG in these systems a detailed study investigating the activity of the microbial community is warranted. In this study we hypothesize microbial activity within the sediment will correlate with N2O emissions. To test the response of the microbial community a combination of molecular approaches (i.e., qPCR and Ion torrent) targeting archaeal and bacterial nitrifiers and denitrifies was used. These functional responses were evaluated with respect to N2O emissions, which were measured in the field at the time of sampling using Unisense N2O probes. In this study, N2O sensor response was calibrated to a functional gene index for rapid risk assessment of GHG hotspots.

Visualization Network Analysis of Studies on Agricultural Drainage Water Treatment

Excessive chemical substances in agricultural drainage water have serious adverse effects on the ecological environment of the watershed into which they are discharged. Therefore, it has attracted widespread attention from scholars worldwide. In this paper, 282 scientific articles related to agricultural drainage water treatment are selected from the Web of Science Core Collection database, and CiteSpace was used to visualize and analyze the knowledge map of this field. The most productive authors, institutions, and countries in agricultural drainage water research are graphically presented in this paper. Developing countries are becoming the core force in this realm of inquiry. In addition, this paper explains the changes in research topics in this field over time and reveals current research hotspots, including “desalination”, “denitrification”, and “phosphorus removal”. Future research endeavors in using bioreactors and agricultural drainage water ditches for treating agricultural drainage water are implied to become a research focus in this field. This paper also emphasizes that future environmental protection research should increase case studies in developing countries and develop corresponding solutions based on the actual situation of agriculture in rural areas of developing countries.

Highlights on Insects and Fungi Associated with Mosquitoes Inhabiting Agricultural Drain Water in Different Areas of Assiut Governorate, Egypt

Highlights on Insects and Fungi Associated with Mosquitoes Inhabiting Agricultural Drain Water in Different Areas of Assiut Governorate, Egypt

Impacts of agricultural drainage on the quantity and quality of tropical peat soil organic matter in different types of forests

Tropical peatlands serve as huge carbon (C) pools in the global C balance, and degradation due to agricultural use is a major concern. The objective of the present study was to examine the relationship between the C loss rate in agriculturally drained tropical peat soils in relation to groundwater level (GWL) and the chemical structure of the resulting soil organic matter (SOM). Soil mesocosms containing three peat soils collected from three forest types in Malaysia, Mixed peat swamp (MPS), Alan Batu (ABt), and Alan Bunga (ABg) forests, which are classified based on dominant plant species, species diversity, and forest structure, were buried in an oil palm plantation for a period of five years. Soil samples packed in the top layer (0–20 cm depth) and the bottom (60–80 cm depth) layer were then analyzed and the results were compared with the initial values for these soils. Weight loss, total C and N contents, ash content, and pyrophosphate solubility index (PSI; an index of the degree of humification) were measured. Changes in the chemical structure of the SOC were investigated using solid-state 13C nuclear magnetic resonance (NMR) spectroscopy with phase-adjusted spinning side bands and thermally assisted hydrolysis and methylation-gas chromatography/mass spectrometry (THM-GC/MS). The C loss in the MPS forest soil, 2–12%, was smaller than that in the ABt and ABg forest soils, 20–37%, especially in the bottom layer. The higher C loss in the ABt and ABg soils, without significant difference between the top and bottom layers, can be attributed to the greater accumulation of less decomposed plant components as evidenced by the larger yields of lignin-derived phenols and polysaccharide-related compounds in THM-GC/MS analysis. The C composition as estimated by 13C NMR did not vary during incubation of the ABt and ABg soils while the yields of lignin-derived and other phenols as well as polysaccharide-related compounds in THM-GC/MS were decreased, especially in the top layer. These findings suggest that plant components with a low degree of decomposition are susceptible to drainage regardless of their structure. The progression of the oxidative degradation of lignin in the top layer of all soils was also suggested by the increases in PSI and the vanillic acid/vanillin ratio. The % O-alkyl C and % alkyl C may control the rate of C loss positively and negatively, respectively. PSI also appears to be useful for roughly estimating the decomposability of tropical peat SOM in various GWLs.

Mapping of the Vulnerability to Water Contamination of the Kharroub River Dam (Northern Morocco) by Application of the SW Method

Pollution and contamination pose a permanent risk of limiting water resources. Urbanization and economic development contribute to the degradation of water quality. The preservation of this natural resource is a major necessity, especially in arid and semi-arid areas where surface water is very scarce. This work constitutes part of this environmental concern. It aims at mapping the vulnerability to contamination of the surface water of the Kharroub River dam (Morocco) and protecting its water resources. The method applied is the SW (Surface Water) method, which consists in elaborating thematic maps of three parameters: the hydric functioning of soils, the density of the hydrographic network and the importance of agricultural drainage. The realization and superposition of these maps allowed us to obtain the vulnerability map to surface water contamination of the Kharroub river watershed. The results obtained show that the low and medium vulnerability classes, representing 41.91% and 32.49% respectively, occupy most of the study area and are distributed throughout the basin. The areas presenting a high and a very high degree of vulnerability occupy respectively 24.65% and 0.95% of the surface of the watershed; they are located principally in the northern and the southern parts of the basin.

Evaluation of drainage water quality for irrigation reuse in Kulfo and Hare irrigation command areas, southern Ethiopia

Abstract Water from agricultural drainage systems can be reused when its quality is good or blended with irrigation canal water to overcome the water shortage. The primary goal of this research was to determine the quality of surface drainage water in the Kulifo and Hare irrigation projects for irrigation reuse. Water quality was evaluated in situ and in the laboratory during the irrigation season of 2022. Turbidity, TDS, pH, EC, and DO were analyzed in the field using an Aqua meter. Fifty-seven water samples were collected and analyzed for the major cation (Na+, Ca2+, K+, and Mg2+) and anion (HCO3−, CO32−, Cl−, and SO42−). The result of the drainage water quality index study, according to the water quality index for irrigation purpose reuse, ranged from 47.84 to 84.89. This indicated that the suitability of drainage water reuse for irrigation purposes was categorized as ‘poor to very poor,’ except in Shara community-managed farms. Therefore, to avoid the impact on soil quality for crop production due to the hazard of poor agricultural drainage water for irrigation reuse in the study area, it needs to be treated before being reused for irrigation purposes, except at the Shara irrigation community-managed farm.

Investigation of the reusability of soilless agricultural drainage water

In greenhouses, the nutrient-rich drainage water is frequently released into surrounding areas, raising pollution issues. Soilless agricultural systems, which have an increasing trend in greenhouse industries, are ideal for recycling water and nutrients because the drainage can be easily captured for reuse. This study was conducted to investigate the reusability of drainage water discharged from an open-system soilless agricultural greenhouse. For this purpose, tomato plants were grown in cocopeat environments. Tomato plants were grown by irrigation mixing different ratios of the drainage water obtained from the open system soilless agriculture greenhouse and the nutrient solutions used for growing plants in soilless agriculture. The study was carried out in five cocopeat environments with four tomato plants in each cocopeat according to a completely randomized experimental design. Some physiological characteristics, yield parameters and the nutritional status of the tomato plants and their fruits were determined. The highest N (5.54%) and K (4.96%) contents in tomato plant leaves were obtained in 100% drainage water applications, while the highest P content (0.84%) was obtained in 100% solution applications. The highest plant height was determined at 100% drainage water application. The highest yield per plant (915.67 g plant−1) and Class I marketable fruit sizes were determined at a 50% drainage and 50% solution mix ratio. No nutrient deficiency or toxicity was detected in tomato plants grown using drainage water. It was concluded that the drainage water discharged from the system can be re-used in greenhouses where open system soilless agriculture is used.

Adaptive irrigation management by Balinese farmers reduces greenhouse gas emissions and increases rice yields.

The potential for changes in water management regimes to reduce greenhouse gases (GHG) in rice paddies has recently become a major topic of research in Asia, with implications for top-down versus bottom-up management strategies. Flooded rice paddies are a major source of anthropogenic GHG emissions and are responsible for approximately 11% of global anthropogenic methane (CH4) emissions. However, rice is also the most important food crop for people in low- and lower-middle-income countries. While CH4 emissions can be reduced by lessening the time the plants are submerged, this can trigger increased emissions of nitrous oxide (N2O), a more potent GHG. Mitigation options for CH4 and N2O are different, and minimizing one gas may increase the emission of the other. Accurate measurement of these gas emissions in rice paddies is difficult, and the results are controversial. We analysed these trade-offs using continuous high-precision measurements in a closed chamber in 2018-2020. Based on the results, we tested a bottom-up adaptive irrigation regime that improves nitrogen uptake by rice plants while reducing combined GHG emissions and nitrogen runoff from paddies to reefs in agricultural drainages. In 2023, we undertook a follow-up study in which farmers obtained higher rice yields with adaptive intermittent irrigation compared to uniformly flooded fields. These results use the polycentric, self-governing capacity of Balinese subaks for continuous adaptation. This article is part of the theme issue 'Climate change adaptation needs a science of culture'.

Agricultural Drainage Water Assessment for Possible Reuse: A Case Study from Pandamatenga Commercial Arable Farms in Botswana

This study set out to assess the quality of water in the drainage channel located in the northern plain of the Pandamatenga Commercial Arable farms in Botswana for possible agricultural reuse. This was achieved by analysing the physical, microbiological and chemical parameters of sampled Agricultural Drainage Water along the drainage channel. The results obtained from the Agricultural Drainage Water quality analysis were used in an Irrigation Water Quality Index model to assess the possibility of reusing the water. The Irrigation Water Quality Index results from the study revealed that the values computed from the five parameters of Sodium Adsorption Ratio, Electrical Conductivity, Sodium, Chloride and Hydrogen carbonate, ninety five percent of the samples fell within the “severe restriction” category, five percent of the samples fell under the “moderate restriction” and no samples belonged to the “no restriction” category. The low levels of Electrical Conductivity and Sodium Adsorption Ratio detected in the water samples during the study period indicated a mineral imbalance, hence making the Agricultural Drainage Water unsuitable for direct reuse. Therefore, using this Agricultural Drainage Water will require mixing it in proper ratios with groundwater to improve its quality for reusability during irrigation. If the Drainage Water is reused, this will have potential to increase the water for irrigation in semi-arid Botswana.

The role of agricultural drainage, storm-events, and natural filtration on the biogeochemical cycling capacity of aquatic and sediment environments in Lake Erie's drainage basin

Lake Erie is the most at risk of the Great Lakes for degraded water quality due to non-point source pollution caused by agricultural activities in the lake's watershed. The extent and temporal patterns of nutrient loading from these agricultural activities is influenced by the timing of agronomic events, precipitation events, and water flow through areas of natural filtration within the watershed. Downstream impacts of these nutrient loading events may be moderated by the co-loading of functionally relevant biogeochemical cycling microbial communities from agricultural soils. This study quantified loading patterns of these communities from tile drain sources, assessed whether functional communities from agricultural sources influenced downstream microbial functionality, and investigated how distance from agricultural sources, storm events, and areas of natural filtration altered nutrient cycling and nutrient fluxes in aquatic and sediment environments. Water and sediment samples were collected in the Wigle Creek watershed in Ontario, from tile drains through to Lake Erie, from May to November 2021, and microbial nitrogen (N) and phosphorous (P) cycling capacity (quantitative PCR), and nutrient levels were evaluated. Results showed that N and P functional groups were co-loaded with nutrients, with increased loading occurring during storm events and during agricultural activities including fertilization and harvest. Overall functional capacity in the aquatic environment decreased with distance from the agricultural sources and as water transited through natural filtration areas. In contrast, the sediment environment was more resilient to both agricultural disturbances and abiotic factors. This study expands our understanding of when and where different stages of N and P cycling occurs in agriculturally impacted watersheds, and identifies both seasons and regions to target with nutrient mitigation strategies.

Importance of Dense Aquatic Vegetation in Seasonal Phosphate and Particle Transport in an Agricultural Headwater Stream

AbstractAgricultural headwater streams and ditches commonly host dense stands of aquatic vegetation that grow and decay over seasons and exert physical and biological controls on the transport of nutrients from cropland to larger rivers. This study examined changes in the transport of phosphorus (P) in an agricultural drainage ditch in the Maumee River Basin (Ohio, USA) by conducting constant rate injections of a novel tracer mixture (conservative salt [Cl as NaCl], dissolved P [KH2PO4], and a fluorescent fine particle) in spring, summer, and fall. We quantified transport behavior for solutes and particles using a traditional transient storage modeling framework consisting of mobile and immobile storage zones connected by a first‐order exchange rate constant. Transient storage was greatest during the spring, when thicker vegetation caused more pooling and flow stagnation, and decreased through fall, as vegetation thinned. Soluble P uptake lengths were 8.7 times longer in fall than spring, likely due to declines in biological uptake rates with colder temperatures and immobile zone storage with thinning vegetation. Particle capture lengths also decreased by a factor of 4.3 from fall to spring. With the increasing eutrophication of Lake Erie and waterbodies around the world that lie downstream from agricultural landscapes, it is beneficial to understand nutrient transport across watersheds, including small agricultural streams. This study highlights the physical and biological roles that aquatic vegetation plays in small agricultural streams by creating seasonally variable immobile zones that slow the flow of nutrients, providing surface area for biofilms, and capturing particles that bind nutrients.

Assessment of Biological Treatment for Ammonia Removal from Water Resources, Egypt

Egypt is depending entirely on the Nile water. The Rosetta branch of the river Nile serves as a vital freshwater source for domestic, agricultural, industrial, fisheries, and recreational purposes in several western Delta governorates of Egypt, with a daily flow averaging 21,500,000 m3/day. The branch faces significant challenges due to escalating ammonia concentrations stemming from agricultural drains along the river, industrial effluents from industrial activities, and fish farming cages. One particular issue arises during low-demand periods when the flow in the Rosetta branch diminishes. Annually, Egyptian authorities close water flows in a series of channels to facilitate maintenance, resulting in reduced water levels. This winter closure has impacted Kafr El Sheikh and El Beheira, located in northern Egypt, as the drainage of industrial wastes during this period has led to a high pollution load of ammonia and other pollutants. The elevated ammonium content has hindered coagulation, encouraged algal growth, and impeded the chlorine breakpoint during water chlorination processes. Laboratory experiments were conducted to investigate the ability of blue-green algae species (Anabaena sp., Scyndesmos sp., Chlorella sp.) and bacteria (Lactobacillus sp.) to uptake ammonia. This study revealed that single algal species achieved a maximum removal percentage of 30% for ammonia concentrations ranging from 5 to 7.5 mg/l, and mixed algal species attained removal percentages between 6% and 12% at constant retention times of 15 to 60 minutes. For single algal species, retention times exceeding 1 hour (2 and 5 hours) resulted in removal percentages ranging from 85% to 100%. For mixed algal species, removal percentages between 9% and 20% were observed for various retention times ranging from 15 minutes to 1 hour. When comparing the use of algal species mixtures, removal percentages of up to 30-50% were achieved. Additionally, the study found that the application of bacterial species (Lactobacillus sp.) to raw water samples with an average ammonia concentration of 7.8 mg/l resulted in a maximum removal percentage of 100% when using a bacterial content of 100-200 ml per 1-liter raw water sample volume, with varied retention times up to 2 hours.

Denitrifying Woodchip Bioreactors: A Microbial Solution for Nitrate in Agricultural Wastewater-A Review.

Nitrate (NO3-) is highly water-soluble and considered to be the main nitrogen pollutants leached from agricultural soils. Its presence in aquatic ecosystems is reported to cause various environmental and public health problems. Bioreactors containing microbes capable of transforming NO3- have been proposed as a means to remediate contaminated waters. Woodchip bioreactors (WBRs) are continuous flow, reactor systems located below or above ground. Below ground systems are comprised of a trench filled with woodchips, or other support matrices. The nitrate present in agricultural drainage wastewater passing through the bioreactor is converted to harmless dinitrogen gas (N2) via the action of several bacteria species. The WBR has been suggested as one of the most cost-effective NO3--removing strategy among several edge-of-field practices, and has been shown to successfully remove NO3- in several field studies. NO3- removal in the WBR primarily occurs via the activity of denitrifying microorganisms via enzymatic reactions sequentially reducing NO3- to N2. While previous woodchip bioreactor studies have focused extensively on its engineering and hydrological aspects, relatively fewer studies have dealt with the microorganisms playing key roles in the technology. This review discusses NO3- pollution cases originating from intensive farming practices and N-cycling microbial metabolisms which is one biological solution to remove NO3- from agricultural wastewater. Moreover, here we review the current knowledge on the physicochemical and operational factors affecting microbial metabolisms resulting in removal of NO3- in WBR, and perspectives to enhance WBR performance in the future.