China, the most populous developingcountry in the world, facing greatpressures regarding population growth,environmental deterioration and resourcedepletion, has ranked plant biology as one of its highest priorities since theearly 1950s, especially in the area ofagricultural development. Since plantbiotechnology became available, it hasdeveloped rapidly in agriculture-relatedplant biology research.Two crucial innovations – tissue andanther culture and DNArecombination –marked the emergence of plantbiotechnology onto the world stage in the 1960s and 1970s. Unfortunately,China was immersed in the CulturalRevolution during that period, and plantscientists were not allowed to do any basic research, although some researchrelated to crop breeding was permitted.For ~20 years, plant tissue culture andanther culture were among the mostactive research areas in plant science inChina because it was believed to be anefficient tool for plant breeding.Many talented young scientists at thattime devoted their careers to developingand improving the technology [1]. To date, >700 plant species have beencultivated successfully via tissue and/oranther culture [2], and >20 new agronomicvarieties have been obtained using thesetechniques [1].DNArecombination techniques were not introduced into China until theearly 1980s because of a lack of facilities,reagents and personnel with propertraining. Before that, isozyme analysiswith electrophoresis was popular, in part,because people believed that with thisinexpensive and relatively simpletechnique it was possible to investigategenetic as well as physiological changesin breeding and the regulation of plantgrowth. Also, because hybrid rice wasmassively used during that time, therewere demands for molecular markers for screening and quality control of hybrid seeds. During the mid-1980s, the first batch ofvisiting scholars sent to Western countriesafter the Cultural Revolution introducedDNArecombination techniques intoChina. Scientists, mainly those based inthe institutes of the Chinese Academy ofSciences (CAS) such as the Institute ofMicrobiology (Beijing), the ShanghaiInstitute of Plant Physiology, theShanghai Institute of Biochemistry andthe Institute of Genetics (Beijing), startedto work with plasmids, viruses andAgrobacteria. Over about five years, the creation of the National Laboratory of Plant Molecular Genetics (Shanghai)laid the foundation for plant molecularbiological research in China. Onenoteworthy invention from the Institute of Biochemistry by Guang-Yu Zhou, is the technique of transforming foreigngenes via the pollen tube pathway [3].Although this approach has beencontroversial since it was proposed, many transformations via this approachhave proven successful at the molecularlevel in crops such as cotton, rice, wheat,maize and soybean [4].After the introduction of DNArecombination techniques into China,Chinese scientists eagerly undertookprotoplast manipulation in the mid-1980s.More than 50 plant species have beengenetically engineered in China to date [1]. Although research into protoplast manipulation declined after the establishment of plant transformationwith
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