Aglaia Odorata Research Articles

2, 9-deoxyflavonoids (= 3-aryl-1-indanones) from Aglaia odorata: Structure elucidation, biosynthetic pathway and lung protective activity

Six previously undescribed 2, 9-deoxyflavonoids (1/2a, 1/2b, 6, and 7), along with six known compounds (3–5, and 8–10), were isolated from the twigs and leaves of Aglaia odorata. Their structures were determined by a combination of spectral analysis, ECD calculation and enzymatic hydrolysis assay. Surprisingly, (±) aglaindanone E (11a, 11b) were unexpectedly formed as the derivatives of compounds 3–6 when exposed to ambient natural light. Furthermore, the plausible biosynthetic pathway of 2, 9-deoxyflavonoids was proposed and chemically mimicked. In biological activity assay, compounds 1/2a, 1/2b, 4, and 6 showed potential protective effects in the 0.75%CSE-induced BEAS-2B cells injury model.

Natural anti-neuroinflammatory inhibitors in vitro and in vivo from Aglaia odorata

Fifty compounds including seven undescribed (1, 13, 18–20, 30, 31) and forty-three known (2–12, 14–17, 21–29, 32–50) ones were isolated from the extract of the twigs and leaves of Aglaia odorata with anti-neuroinflammatory activities. Their structures were determined by a combination of spectral analysis and calculated spectra (ECD and NMR). Among them, compounds 13–25 were found to possess tertiary amide bonds, with compounds 16, 17, and 19–21 existing detectable cis/trans mixtures in 1H NMR spectrum measured in CDCl3. Specifically, the analysis of the cis–trans isomerization equilibrium of tertiary amides in compounds 19–24 was conducted using NMR spectroscopy and quantum chemical calculations. Bioactivity evaluation showed that the cyclopenta[b]benzofuran derivatives (2–6, 8, 10, 12) could inhibit nitric oxide production at the nanomolar concentration (IC50 values ranging from 2 to 100 nM) in lipopolysaccharide-induced BV-2 cells, which were 413–20670 times greater than that of the positive drug (minocycline, IC50 = 41.34 μM). The cyclopenta[bc]benzopyran derivatives (13–16), diterpenoids (30–35), lignan (40), and flavonoids (45, 47, 49, 50) also demonstrated significant inhibitory activities with IC50 values ranging from 1.74 to 38.44 μM. Furthermore, the in vivo anti-neuroinflammatory effect of rocaglaol (12) was evaluated via immunofluorescence, qRT-PCR, and western blot assays in the LPS-treated mice model. The results showed that rocaglaol (12) attenuated the activation of microglia and decreased the mRNA expression of iNOS, TNF-α, IL-1β, and IL-6 in the cortex and hippocampus of mice. The mechanistic study suggested that rocaglaol might inhibit the activation of the NF-κB signaling pathway to relieve the neuroinflammatory response.

The complete mitochondrial genome of Aglaia odorata, insights into its genomic structure and RNA editing sites.

Aglaia odorata, native to Guangdong, Guangxi, and Hainan provinces in China, has long been utilized as an herbal remedy in ancient China. In this study, we assembled and annotated the complete mitochondrial genome (mitogenome) of A. odorata, which spans a total length of 537,321 bp. Conformation of the A. odorata recombination was verified through PCR experiments and Sanger sequencing. We identified and annotated 35 protein-coding genes (PCGs), 22 tRNA genes, and 3 rRNA genes within the mitogenome. Analysis of repeated elements revealed the presence of 192 SSRs, 29 pairs of tandem repeats, and 333 pairs of dispersed repeats in the A. odorata mitogenome. Additionally, we analyzed codon usage and mitochondrial plastid DNAs (MTPTs). Twelve MTPTs between the plastome and mitogenome of A. odorata were identified, with a combined length of 2,501 bp, accounting for 0.47% of the mitogenome. Furthermore, 359 high-confidence C to U RNA editing sites were predicted on PCGs, and four selected RNA editing sites were specially examined to verify the creation of start and/or stop codons. Extensive genomic rearrangement was observed between A. odorata and related mitogenomes. Phylogenetic analysis based on mitochondrial PCGs were conducted to elucidate the evolutionary relationships between A. odorata and other angiosperms.

Phenolic and bisamide derivatives from Aglaia odorata and their biological activities

Three new compounds (1–3), including two bisamide derivatives (1 and 2) and a lignin (3), along with 15 known compounds were isolated from Aglaia odorata. Compound 2 was a pair of enantiomers and successfully resolved into the anticipated enantiomers. Their structures were elucidated by extensive spectroscopic analysis, electronic circular dichroism (ECD) calculations, and X-ray crystallography. Three compounds showed excellent inhibitory activities on α-glucosidase with IC50 values ranging from 54.48 to 240.88 μM, better than that of the positive control (acarbose, IC50 = 590.94 μM). Moreover, compounds 3, 13, and 15 presented moderate inhibitory activities against butyrylcholinesterase. Compound 17 exhibited potent PTP1B inhibitory activity with an IC50 value of 179.45 μM. Representative active compounds were performed for the molecular docking study. Herein, we described the isolation, structure elucidation, the inhibitory effects on three enzymes, and molecular docking of the isolates from the title plant.

Profil Fitokimia dan Uji Toksisitas Daun Tumbuhan Aglaia Odorata dengan Metode Brine Shrimp Lethality Test

Toxicity and phytochemistry assay have been conducted on extracts and fractions of Aglaia odorata leaves using brine shrimp lethality test. Aglaia odorata is one of the plants that are found in Indonesia. To investigate Aglaia odorata leaves potential as anticancer, air-dried and powdered leaves were extracted with n-hexane, ethyl acetat, and metanol by using maceration method. Extract of ethyl acetat showed the highest toxicity with LC50 value is 125.89 mg/L

Isolation and synthesis of rocaglaol derivatives by inhibiting Wnt/β-catenin and MAPK signaling pathways against colorectal cancer

Eight rocaglaol derivatives with good cytotoxic activity (IC50: 0.013 ∼ 5.82 μM) were isolated from Aglaia odorata. Then, a series of novel derivatives with modifications on C3 of rocaglaol were designed, synthesized, and screened for their antitumor activities against three tumor cell lines (HEL, MDA-MB-231, and HCT116). A total of 44 derivatives exhibited significant cytotoxic activity with IC50 values lower than 1 μM. In particular, four derivatives (14, 20, 22j, and 22r) exhibited the best cytotoxic activity against HCT116 cells, with an IC50 value of 70 nM. Compound 22r with relatively low toxicity against normal cells and the best cytotoxic activity against HCT116 cells was selected for further study. Subsequent cellular mechanism studies showed that compound 22r induced apoptosis and G1 cell cycle arrest in HCT116 cells. Moreover, compound 22r inhibited both the Wnt/β-catenin and MAPK signaling pathways via key proteins, such as the phosphorylation of p38 and JNK, GSK-3β, Axin-2, etc. Therefore, our present results suggest that compound 22r is a potential candidate for developing novel anti-colorectal cancer agents in the future.

Three New Aglain Derivatives from Aglaia odorata Lour. and Their Cytotoxic Activities.

Three new aglain derivatives (1-3), one known aglain derivative (4), two known rocaglamide derivatives (5 and 6), four known triterpenoids (7-10), and three steroids (11-13) were isolated from Aglaia odorata Lour. Their structures were established through the analysis of detailed spectroscopic data and electronic circular dichroism calculations. Five compounds (1 and 4-7) exhibited cytotoxic activities on human leukemia cells (HEL) and human breast cancer cells with IC50 values in the range of 0.03-8.40 μM. In particular, the cytotoxicity of compound 5 was six times stronger than that of the positive control (adriamycin) in HEL cell lines.

Toxicology of Nuclear Polyhedrosis Virus, Botanical and Synthetic Pesticides on Mortality Rate of Crocidolomia binotalis (Zeller)

Crocidolomia binotalis (Cb) (Zeller) is one of the main pests of cabbage which is difficult for farmers to control in the Ijen Crater in Indonesia. The demand for cabbage in Indonesia is increasing every year, so intensification efforts are continuously being made. Farmers intensively use synthetic pesticides as the primary control of Cb. The purpose of this study is to examine several control agents that can be used as an alternative to synthetic pesticides. Then, it provides recommendations to Ijen Crater farmers to adapt in reducing the use of synthetic pesticides. The biopesticide used was based on the Nuclear Polyhedrosis Virus strains of Spodoptera litura (SlNPV) and Helicoverpa armigera (HaNPV), botanical pesticides, and synthetic pesticides as a comparison treatment. SlNPv and HaNPV isolates were obtained from the Indonesian Sweetener and Fiber Crops Research Institute. The botanical pesticide formulas come from a combination of Azadiracta indica, Aglaia odorata, Ageratum conyzoides dried for one week and proposed to form flour. Then, it was extracted using 96% ethanol solvent, 0.5% tween 90 using a rotary evaporator at a temperature of 40oC. For synthetic pesticide, the treatment used the active ingredient Permethrin 20 mgL-1. The cb collected from the field was re-filtered in a Petri dish by providing artificial feed soaked with this material for 5 minutes. The highest mortality test results were found in the botanical pesticide formula with a value of 100% at 72 hours. Mortality in SlNPV and HaNPV tended to be stable with a maximum value of 72.02%. The results of mortality testing in the laboratory can be a recommendation for alternative control of Cb pests in cabbage plants in the field. In this case, it is an effort to reduce farmers' dependence on using synthetic pesticides.

A review of application of natural products as fungicides for chili

Anthracnose disease in chillies is a serious problem for farmers. So far, synthetic fungicides have been used as solution for the treatment of this disease. However, the side effects of synthetic fungicides to public health and environment raised awareness on alternative fungicides derived from natural resources. This paper aims to review plants that are potential as an alternative to fungicides for chili plantation, fabrication of test solutions, in vitro and in vivo fungicide test. Many plants were investigated as alternatives to plant-based fungicide. The utilization of leaves as samples including rhizomes, roots, tubers, weevils, seeds, fruit, flowers and other parts of the plant. The extract fabrication method used as a fungicide test include: maceration method, gradual fractionation method, and decoction method. The maceration method is the method most widely used to extract fungicidal active compounds from plants. Some studies that carried out in vitro tests were unable to compare with synthetic fungicides so it was not possible to determine their effectiveness for plant-based fungicide for chillies when compared to synthetic fungicides. In vitro Extract of 80% alcohol and 10%/60% n-hexane of pacar cina (Aglaia odorata L.) leaves can be compared with the performance of propineb 0.2%. In addition, the 60% and 70% kirinyuh (Chromolaena odorata L.) leaf extracts were also able to match Acrobat 0.2% performance in vitro. Based on the in vivo test, suren (Toona sureni Merr) leaf extract and nut bulbs can be used as an alternative to vegetable / natural fungicides to help overcome the problem of anthracnose in chilies.

In vitro studies on Bacillus sp. and Pseudomonas sp. compatibility with botanical pesticide

The effect of botanical pesticides as an insect pest control agent has been proven in several studies. Despite capable of controlling pests, botanical pesticides have not been reported to promote plant growth. One technique for making botanical pesticides served in dual function is to combine them with beneficial microbes. This study was aimed to determine the compatibility of 3 isolates of Bacillus sp. and 3 isolates of Pseudomonas sp. against botanical pesticides. The botanical pesticides were made from a mixture of Ageratum conyzoides, Aglaia odorata, and Azadirachta indica leaves. The solvent used 96% ethanol, and the process of making botanical pesticides was performed using a rotary evaporator at 45˚C. The compatibility test was performed in vitro by dropping 10 µl of botanical pesticides on the bacterial culture. Tests were carried out using various concentrations, namely 0%, 25%, 50%, 75%, and 100%. The test results showed that Bacillus sp. A and Pseudomonas sp. E strain were compatible on all assay concentrations. Bacillus sp. A and Pseudomonas sp. D and Pseudomonas sp. E were compatible with the concentrations of 0 to 75%. Furthermore, Bacillus sp. B, Bacillus sp. C, and Pseudomonas sp. F were compatible with the assay concentrations of 0 to 50%.

Combination of plant growth-promoting bacteria and botanical pesticide increases organic red rice yield and reduces the Leptocorisa acuta population

Abstract. Hoesain M, Prastowo S, Suharto, Pradana AP, Asyiah IN, Alfarizy FK, Adiwena M. 2021. Combination of plant growth-promoting bacteria and botanical pesticide increases organic red rice yield and reduces the Leptocorisa acuta population. Biodiversitas 22: 1686-1694. Organic red rice production faces similar constraints as conventional rice production, namely pest attacks, especially rice earhead bugs (Leptocorisa acuta). The use of synthetic chemical inputs is also not allowed in organic red rice production. This problem can be overcome with the application of botanical pesticides, but the use of botanical pesticides do not promote plant growth. As a solution, a combination formula of plant growth-promoting bacteria and botanical pesticides is required. This study aimed to examine the effectiveness of a combination of plant growth-promoting bacteria with botanical pesticides in increasing the growth and yield of organic red rice plants and reducing the rice earhead bug population. The research was conducted on organic agricultural land in Jember Regency, Indonesia. The research began with the extraction of botanical pesticides from the leaves of Azadirachta indica, Aglaia odorata, and Ageratum conyzoides using 96% ethanol. The bacterial isolates obtained and characterized in previous studies were tested for their compatibility and cell viability in the combination formula. The field experiment was conducted with 8 treatments, 3 replications, and each replication consisted of 150 rice plants. The randomization pattern followed a randomized block design pattern. The results showed that all bacteria were compatible when combined with 20% botanical pesticides. In addition, cell viability at 3 months of storage decreased, but not significantly. In the organic rice growth variable, all combinations of bacteria with botanical pesticides showed good performance to increase the plant growth. Increased plant growth occurred in the number of tillers, plant height, and the number of productive panicles. Furthermore, yield variables, fresh seed weight per panicle, dry seed weight per panicle, and weight of 1000 seeds also increased significantly after being treated with a combination of plant growth-promoting bacteria and botanical pesticides. The decline in the rice earhead bug population also occurred in all plants treated using botanical pesticides either singly or in combination. The best treatments in this study were BS01 and PD01, which showed consistent results. This study provides information that the combination of plant growth-promoting bacteria and botanical pesticides is compatible, does not cause a high number decrease of bacterial cells at a shelf-life of 3 months, and can stimulate the growth and yield of organic red rice, and can suppress rice earhead bug populations.

The Selection of Ornamental Plant for Landscape Design of Pollination Garden at Bogor Botanic Gardens

Bogor Botanic Gardens is one of the outstanding green areas in Bogor City. Thousands of plant species are growing and providing suitable habitats and feed for various pollinators, such as butterflies, bees, and wasps. A well-designed pollination garden will benefit the pollinators and also create an educational facility for the community. This study aimed to select and characterize species of flowering plants that effectively attract pollinators, particularly Lepidoptera and Hymenoptera, in the pollination garden candidate area. The observa-tion had been carried out on 12 ornamental plant species: Pennisetum purpureum, Cosmos sp., Pachystachys lutea, Cuphea hyssopifolia, Orthosiphon aristatus, Lantana camara, Citrus aurantifolia, Buddleja davidii, Ixora javanica, Nerium oleander, Hibiscus rosa-sinensis, and Aglaia odorata. The method for observing pollinators was a standard walking method. Pollinator photos, its number, its visit time, and the plants it visited were recorded. A total of 23 species of Lepidoptera (20 species from 4 families and 3 species were unidentified) and 9 species of Hymenoptera (4 families) were found in the area with the best obser-vation time at 08.30-09.30 (GMT+7). The most visited plants by pollinators were Cuphea hyssopifolia, Lantana camara and Orthosiphon aristatus. Lepidoptera and Hymenoptera preferred blue-purple and bright-orange-yellow flowers. In addition, the flower characters that might influence pollinator visits are also described. The result would be an essential recommendation for plant selection, landscape design, and construction of the pollination garden in Bogor Botanic Gardens.

KATEGORI KONSERVASI VEGETASI TUMBUHAN BAWAH DI LAHAN BERA WOMNOWI DISTRIK SIDEY MANOKWARI PAPUA BARAT

Trees vegetation are obviously dominate at the old fallow lands of Papua Indonesian. Fallow lands in the edge of primary forest is generally at Sidey District Manokwari. The purpose of this study is to determinate understory cover vegetation conservation based list of IUCN at the fallow land Womnowi Sidey Manokwari. An inventory of vegetation has done using analysis of vegetation―continuous line sampling technique, 2 x 2 meters for sampling seedlings and understory non-woody plant cover and 5 x 5 for saplings. At one hectare fallow land we found 1482 an individual of 122 species understory cover, only 158 an individual of 22 species had entered in IUCN redlist. Species with status least concern (LC) are dominate (>80%) compare with status data deficient (DD), near threatened (NT), and vulnerable (V). The important value index (IVI) of species on list IUCN showing 22.60% at seedlings and non-woody understory cover and 19.81% at the saplings phase. Aglaia odorata Lour.(seedling and sapling) is LC category, Intsia bijuga (Colebr.) Kuntze (seedling) V category, and Pandanus tectorius var., uapensis (non-woody plant) DD category, each species is the only one. The further study should be more intensive compare primary forest and old fallow lands of Papua―the conservation list of understory cover vegetation is lowest, so we conclude this is obviously understory vegetation at old fallow lands.Key word: fallow land, conservation, Sidey, understory, analysis of vegetation

Aglaia odorata Lour. extract inhibit ischemic neuronal injury potentially via suppressing p53/Puma-mediated mitochondrial apoptosis pathway.

Aglaia odorata Lour. is a traditional Chinese medicinal plant possessing properties of improving blood circulation, and it is widely used in the treatment of dizziness, traumatic injuries and bruises. In this study, we are aimed to investigate the cerebral protection effect of the extracts from leaves of Aglaia odorata Lour. (ELA) and the potential mechanism in vivo and in vitro. The therapeutic effect of ELA on ischemic cerebral stroke was measured on a middle cerebral artery occlusion (MCAO) rat model. Protective effect of ELA on oxygen-glucose deprivation/reperfusion (OGD/R)-induced PC12 cells was measured by MTT assay. The apoptotic cells were observed by Hoechst 33258 staining and acridine orange/ethidium bromide double staining assay. Mitochondria were observed by Mitotracker staining assay. The mitochondrial membrane potential was determined by JC-1 staining assay. Western blot was used to investigate the effects of ELA on apoptosis-related proteins. We showed that ELA was an effective neuroprotective agent. In vivo experiments, ELA exerted significant protective effect on MCAO model. TTC staining showed that ELA could reduce cerebral infarction area against MCAO insult. HE and Nissl's staining indicated that ELA could reverse the damage of cortex and hippocampus caused by MCAO. In vitro experiments, ELA showed significant protective effect on OGD/R-induced PC12 cells by reducing the number of apoptotic cells, increasing mitochondrial membrane potential, and reducing superoxide aggregation, further suppressing mitochondrial caspase-9/3 apoptosis pathway. Moreover, protective effect of ELA on mitochondrial function may be exerted by inhibiting p53/Puma signal pathway. Our results suggest that ELA exerts a marked neuroprotective effect against cerebral ischemia potentially via suppressing p53/Puma-mediated mitochondrial caspase-9/3 apoptosis pathway.

TOKSISITAS SENYAWA Β-SITOSTEROL DAN EKSTRAK KULIT BATANG AGLAIA

Pacar cina (Aglaia odorata Lour) is a plant that is widely used as an traditional medicine because of natural materials they contain. The study aims to from Pacar cina extracts and toxicity test of each extract and isolated compound by BSLT (Brine Shrimp Lethality Test) method. The method used in the maceration. In this study, the n-hexane, ethyl acetate, methanol extracts and β-sitosterol compound showed toxic effect (LC50 261,17; 110; 266,75 and 100 μg/mL, respectively). The toxicity degree among the three extracts and isolated compound is β-sitosterol > ethyl acetate > n-hexane > methanol.

Identification of 4-4’-(1-methylethylidene)-bisphenol from an Endophytic Fungus Cladosporium oxysporum derived from Aglaia odorata

<p>Endophytic fungi has an economic potential as raw material for biologically active compounds<em>. Cladosp</em><em>o</em><em>rium oxysporum</em><em> </em>is one of the endophytic fungi isolated from Indonesian medicinal plant <em>Aglaia odorata </em>Lour (Local name: Pacarcina). This planth has been used for fever, cough, diarrhea, inflammation and injury. In our previous study, the ethyl acetate extract and several fractions of the extract of <em>C. oxysporum </em>showed antimicrobial activity against <em>Candida albicans, Escherichia coli</em>, and <em>Staphylococcus aureus</em>. The objective of the currents study is to investigate the chemical constituent of the active fraction.<strong> </strong>Purification of the metabolite was achieved by using column chromatography followed by preparative thin layer chromatography. Identification of the metabolite was conducted by using TLC densitometry, GC-FID and GC-MS. Compound 1 was isolated from fraction 12. The purity of this compound was determined by 2D-TLC and GC-FID. The UV-Vis profile of compound 1 indicates a phenolic compound. Further analysis by using GC-MS shows one peak at a retention time (Rt) of 23.80 minutes, predicted as 4-4’-(1-methylethylidene)-bisphenol. The chemical constituent of the sub fraction 12.2.7 (fraction <strong>a</strong>) is identified as 4-4’-(1-methylethylidene)-bisphenol.</p>

PENGARUH FRAKSI EKSTRAK DAUN PACAR CINA (AGLAIA ODORATA L.) TERHADAP PERTUMBUHAN COLLETOTRICHUM CAPSICI PENYEBAB PENYAKIT ANTRAKNOSA PADA CABAI (CAPSICUM ANNUUM L.) SECARA IN VITRO

Influence of Aglaia odorata L. leaf extract on in vitro growth of chilli antrachnose fungi (Colletotrichum capsici). Pacar cina (Aglaia odorata L.) is known as one of the potential herbs as fungicides. The objective of this study was to determine The effectiveness of various leaf extract fractions of pacar cina as a fungicide to suppress the in vitro growth of C. capsici, The causal agent of anthracnose disease on chili. This study consisted of two sub-experiments. The first experiment consisted of 12 treatments: leaf extract fractions with distilled water solvent, leaf extract fractions with ethanol solvent with a concentration of 10, 20, 30, 40, 50, 60, 70, 80 and 90%, propineb 0.2 % (synthetic fungicides) and control (without fungicide). The Second experiment consisted of 12 treatments: leaf extract fractions with distilled water solvent, leaf extract fractions with the solven of n-hexane at a concentration of 10, 20, 30, 40, 50, 60, 70, 80 and 90%, propineb 2% (synthetic fungicides), and control (without fungicides). The treatmentswerearranged by completely randomized design (CRD) with three replications. Variables measured in this experiment were colony diameter and density of spores of C. capsici. Data were analyzed by analysis of variance (ANOVA) and differences between the mean values was tested by Duncan Multiple Range Test (DMRT) with the level of 5% significant different. Additionally, leaf extract that effectively inhibited the growth of C. capsici was the fraction of the leak extract with 80% ethanol solvent and 10%, 60%, and 90% n-hexanesolvent. The result showed that the leaf extract fraction of pacar cina was potentially used as fungicide to inhibit the growth of C. capsici.

A Potent Phytotoxic Substance in Aglaia odorata Lour.

Aglaia odorata Lour. (Meliaceae) was found to have very strong allelopathic activity and a bioherbicide PORGANIC(™) was developed from its leaf extracts. However, the phytotoxic substances causing the strong allelopathic activity of the plants have not yet been determined. Therefore, we investigated allelopathic properties and phytotoxic substances in A.odorata. Aqueous EtOH extracts of A.odorata leaves inhibited root and shoot growth of garden cress (Lepidum sativum), lettuce (Lactuca sativa), alfalfa (Medicago sativa), timothy (Phleum pratense), ryegrass (Lolium multiflorum), and Echinochloa crus-galli with the extract concentration-dependent manner. The extracts were then purified and a major phytotoxic substance with allelopathic activity was isolated and identified by spectral data as rocaglaol. Rocaglaol inhibited the growth of garden cress and E.crus-galli at concentrations >0.3 and 0.03μm, respectively. The concentrations required for 50% inhibition ranged from 0.09 to 2.5μm. The inhibitory activity of rocaglaol on the weed species, E.crus-galli, was much greater than that of abscisic acid. These results suggest that rocaglaol may be a major contributor to the allelopathic effect of A.odorata and bioherbicide PORGANIC(™) .

Beberapa Aspek Biologi Parasitoid Apantheles sp pada Inangnya, Spodopera litura, Fab. setelah Perlakuan Ekstrak Daun dan Ranting Aglaia odorata (Lour)

The objectives of this study were to evaluate effect of leaf and branch extract of the Aglaia odorata against: emergences of adult Apantheles sp emerged from S. litura larvae, (2) the extract effect to live cycle and reproduction of Apantheles sp (3) and its effect against the morphology character some of parasitoid. The metode was used by of leaf-dip method. The leaf and branch effectivity bioassay used by of Ricinus communis leaf . The leaf disk were dipped in the extract solution on six concentration for 10 s, and air dried. Each leaf disks was placed six bottles glass and ten larvae were placed in each botlle, each concentration was replicated four time. Data collected were subjected an anaysis of variance followed by mean comparation based of Duncan´t New Multiple Range Test. The imago Apantheles sp. emerged from host, S. litura larvae was recorded of live cycle, its reproduction, and morphology character. The result showed that the length of pre adult stage of emerging parasitoids from of S. litura larvae treated with 85,99 ppm was 45 percent, and with 21,95 ppm was 22,22 percent. The extract toxicity to natural enemies such as parasitoids relatively non toxis. The live cycle of female adult parasitoid was 16,90 and 6,20 days. Reproduction capacity of adult female parasitoids of 85,99 mg/L was 69,4 eggs/adult, which was statistically different from control, whereas of 21,95 treatment, it was 65,40 eggs/adult and its was not significant to control. Key words: Aglaia odorata, Lour, Spodoptera litura,, morphology character, Apanteles sp

Cytotoxicity and Synergistic Effect of the Constituents from Roots of Aglaia odorata (Meliaceae)

Twelve compounds were isolated from the roots of Aglaia odorata. Their structures were established on the basis of NMR and MS data as rocaglaol (1), rocaglamide (2), eichlerialactone (3), sapelins A (4), isofouquierone (5), eichlerianic acid (6), shoreic acid (7), agladupol E (8), 3-epimeliantriol (9), cleomiscosins B (10), 2β,3β-dihydroxy-5α-pregnane-16-one (11) and β-d-glucopyranos-1-yl N-methylpyrrole-2-carboxylate (12). Among them, compounds 1 and 2 showed significant cytotoxicity against human cancer cell (HL-60, SMMC-7721, A-549, MCF-7 and SW480) with IC50 values of 0.007–0.095 μM, while compounds 3–5 and 10 and 11 showed moderate to no cytotoxicity (IC50 0.43 to values >40 μM). Compound 6 showed only weak cytotoxicity (IC50 6.87 to >40 μM) and its epmier 7 was completely inactivite (IC50>40 μM) in the assay. However, potent synergistic effect was observed when the molar ratio of 6 to 7 is between 4:1 and 1:1.