Identification of 4-4’-(1-methylethylidene)-bisphenol from an Endophytic Fungus Cladosporium oxysporum derived from Aglaia odorata

Abstract

<p>Endophytic fungi has an economic potential as raw material for biologically active compounds<em>. Cladosp</em><em>o</em><em>rium oxysporum</em><em> </em>is one of the endophytic fungi isolated from Indonesian medicinal plant <em>Aglaia odorata </em>Lour (Local name: Pacarcina). This planth has been used for fever, cough, diarrhea, inflammation and injury. In our previous study, the ethyl acetate extract and several fractions of the extract of <em>C. oxysporum </em>showed antimicrobial activity against <em>Candida albicans, Escherichia coli</em>, and <em>Staphylococcus aureus</em>. The objective of the currents study is to investigate the chemical constituent of the active fraction.<strong> </strong>Purification of the metabolite was achieved by using column chromatography followed by preparative thin layer chromatography. Identification of the metabolite was conducted by using TLC densitometry, GC-FID and GC-MS. Compound 1 was isolated from fraction 12. The purity of this compound was determined by 2D-TLC and GC-FID. The UV-Vis profile of compound 1 indicates a phenolic compound. Further analysis by using GC-MS shows one peak at a retention time (Rt) of 23.80 minutes, predicted as 4-4’-(1-methylethylidene)-bisphenol. The chemical constituent of the sub fraction 12.2.7 (fraction <strong>a</strong>) is identified as 4-4’-(1-methylethylidene)-bisphenol.</p>