Aggressive Behavior Research Articles

PATH-47. AGE-, SEX-, AND GRADE-ASSOCIATED MOLECULAR DIFFERENCES IN A MULTI-INSTITUTIONAL COHORT OF CHOROID PLEXUS TUMORS

Abstract BACKGROUND Choroid plexus tumors (CPT) are central nervous system tumors where prognosis has been correlated to histologic grade, with aggressive clinical behavior and reduced survival rate in higher grade cases. Here, we present a genomic survey of a large multi-institutional cohort of CPTs across all grades. MATERIAL AND METHODS Fifty-eight CPT samples were analyzed by comprehensive genomic profiling (CGP) by a hybrid capture-based DNA sequencing platform (FoundationOne®CDx). Pathology reports, histopathology reviews, and patient clinical data were assessed. RESULTS Our cohort included 11 choroid plexus papillomas (CPP; grade 1), 16 atypical CPP (grade 2), and 31 choroid plexus carcinomas (CPC; grade 3). Males were slightly more affected than females (31 vs. 27 cases). Median patient age at tissue sampling was 11 years (range: 1–72 years). Median age of patients with CPC was younger at 3 years compared CPP at 34 years (p < 0.01). Genomic analyses identified three frequent, mutually exclusive mutations in TP53, TERTp, and DAXX. TP53 mutations (n = 15, 26%) were significantly more prevalent in CPC (n=14 CPC, p=0.0003), and were identified predominantly in children (n=12 CPC at <20 years). By contrast, TERT promoter mutations (TERTp; n = 5, 8.6%) were identified exclusively in CPP (p=0.019), predominantly grade 2 (4 of 5 cases), and exclusively in adult patients, with a median age of 48 years. DAXX mutations (n=4, 6.9%) were more common in CPC (n=3). Other alterations included PTEN (n=4, all adults) and MYCN amplification (n=3, all children <6 years), both exclusively in CPC. In 21 cases, including 9 out of the 11 Grade 1 CPP, no known cancer mutation was detected. CONCLUSION This study provides comprehensive genomic profiling of CPTs, highlighting distinct genetic alterations associated with different histological grades and patient demographics. These findings may inform future research and therapeutic strategies targeting specific molecular pathways in CPTs.

TMIC-02. SINGLE-CELL HIGH-DIMENSIONAL MASS CYTOMETRY (CYTOF) REVEALS IMMUNE LANDSCAPE OF PITUITARY NEUROENDOCRINE TUMORS

Abstract Pituitary neuroendocrine tumors (pitNETs) are a group of neoplasms in the sellar region, ranking as the second most common primary brain tumors, with some exhibiting aggressive behaviors. While immunohistochemical staining has been utilized to characterize the tumor immune microenvironment (TIME) of pitNETs, single-cell analysis remains underexplored. High-dimensional mass cytometry (CyTOF) offers a valuable approach for precise quantification of intratumoral leukocytes and potential therapeutic insights. We prospectively enrolled 56 patients with pitNETs of varying endocrine function, histology, and lineage. CyTOF, employing heavy metal-tagged antibodies, was employed to simultaneously measure 37 cellular parameters at single-cell resolution. Analysis revealed a total of 71,049 CD45+ immune cells. Macrophages (mean, 50.7%) and T cells (mean, 28.7%) were the predominant leukocytes, followed by NK cells, dendritic cells, B cells, and neutrophils. The immune cell composition within the TIME exhibited heterogeneity, with distinct patterns observed between functioning and non-functioning pitNETs. Functioning pitNETs displayed fewer macrophages (28.9% vs. 62.9%) and more T cells (44.9% vs. 19.6%) compared to non-functioning pitNETs. Subgroups within functioning (somatotroph, mammosomatotroph, lactotroph) and those within non-functioning pitNETs (null-cell, gonadotroph, corticotroph) shared similar immune profiles, respectively. PIT-1 lineage pitNETs demonstrated a T cell-dominated pattern, while SF-1, T-PIT, and null-cell lineage pitNETs exhibited macrophage predominance. In functioning pitNETs, macrophage abundance negatively correlated with invasiveness, Ki-67 index, and recurrence, while CD4 T cells showed positive correlations. Further unsupervised clustering identified 27 subpopulations of intratumoral immune cells, with some serving as critical predictors of tumor behavior. Our study provides a comprehensive single-cell immune profile of pitNETs, revealing distinct features across subtypes and targeted therapeutic implications.

Effects of short-term isolation on social behaviors in prairie voles.

Social isolation affects the brain and behavior in a variety of animals, including humans. Studies in traditional laboratory rodents, including mice and rats, have supported the idea that short-term social isolation promotes affiliative social behaviors, while long-term isolation promotes anti-social behaviors, including increased aggression. Whether the effects of isolation on the social behaviors of mice and rats generalize to other rodents remains understudied. In the current study, we characterized the effects of short-term (3-days) social isolation on the social behaviors of adult prairie voles (Microtus ochrogaster) during same-sex and opposite-sex social interactions. Our experiments revealed that short-term isolation did not affect rates of ultrasonic vocalizations or time spent in non-aggressive social behaviors and huddling during same-sex and opposite-sex interactions. Unexpectedly, although short-term isolation also did not affect time spent in resident-initiated and mutually-initiated aggressive behavior, we found that short-term isolation increased time spent in visitor-initiated aggression during male-male interactions. Our findings highlight the importance of comparative work across species and the consideration of social context to understand the diverse ways in which social isolation can impact social behavior.

PATH-59. HISTOLOGIC CORRELATES FOR MOLECULAR FEATURES IN MENINGIOMAS

Abstract Histologic findings are the primary determinant of meningioma prognosis and post-operative management. However, the association of specific pathologic features with molecular alterations has not yet been comprehensively investigated. Here we utilized a large dataset of well-characterized meningiomas to identify correlates between clinically relevant histological and molecular features. Detailed histologic information was collected for 809 resected meningiomas, including cases with DNA methylation (n=380), copy number array (n=252), next-generation sequencing (n=52), RNA risk score (n=394), and/or TERT promoter profiling (n=238). Analyses were conducted on prognostic pathologic features, including WHO Grade, Ki-67, mitotic index (MI), brain invasion, and atypical histologic findings (necrosis, hypercellularity, prominent nucleoli, sheeting, and small cells). Correlation with molecular variables was performed using Fisher’s Exact Tests, including DNA methylation subgroup, RNA risk score, CNV profiles, and TERT promoter mutations. All statistics were corrected for multiple hypothesis testing. Nearly 65% of meningiomas exhibited at least one atypical histologic feature. Among said features, Ki-67/MI, WHO Grade, sheeting, and necrosis were associated with recurrent lesions and known clinical features of aggressive meningiomas. Several histologic features, including hypercellularity, elevated Ki-67/mitotic index, and WHO grade, also correlated with DNA methylation and RNA risk groups. Several chromosomal arm events were related to specific pathologic features (prominent nucleoli, Ki-67, MI, and grade), including chromosomes 1, 4, 10, 14, and 18. Our analysis highlights the continued value in screening for histologic markers of aggressive behavior in meningiomas, as they correlate with the presence of high-risk molecular features and may serve as proxy markers when advanced molecular testing is not available.

Reduction in Restraint and Critical Incidents in a Norwegian Residential Treatment Facility for Children Aged 7–13 Following the Implementation of the Neurosequential Model of Therapeutics

In child and adolescent inpatient, residential and day-treatment facilities, the use of physical restraints and the occurrence of critical incidents are a significant problem. Restraints may sometimes be necessary if a child exhibits dangerous aggressive behavior, but may also be misused or overused, and have been shown to be preventable in many cases. This study aims to investigate if the implementation of the Neurosequential Model of Therapeutics (NMT) has an effect on the annual number of physical restraint incidents in a residential treatment facility for children with complex mental health disorders. Data before and after NMT implementation were collected from the agency’s restraint records. The results showed that post-NMT implementation, there was a substantial and sustained reduction in restraint incidents, with a Cohen’s d value of 2.03, indicating a very large effect. Limiting restraint use in treating children with complex mental health disorders can foster a safer and more therapeutic environment, with potential improvements in treatment outcomes. This study demonstrated a substantial drop in restraint incidents following the implementation of the Neurosequential Model in a residential facility for children aged 7–13. This reduction is presumably pivotal for children with complex mental health disorders, making treatment less coercive and offering promise for settings in which restraint incidents are a concern.

Cyber Dating Violence: How Is It Perceived in Early Adolescence?

Background: Reports on cyber dating violence in adolescent populations vary significantly depending on whether the focus is on directly aggressive behaviours or behaviours designed to control one’s partner. In contrast to direct aggression, which is often clearly identified by adolescents, there is a greater degree of ignorance, and even a certain degree of normalisation, of controlling behaviours. Such behaviours may include, for example, insisting on knowing the whereabouts of a partner at all times or sharing social media passwords. This study aims to explore adolescent perceptions of cyber dating violence and to identify the differential characteristics of cyber-violent relationships using the Iramuteq software program for text analysis. Methods: Participants were 466 second- and third-year secondary school students. Data were collected through surveys, and responses were analysed using the Iramuteq program. This software tool enabled the identification of common terms and themes linked to cyber dating violence, as perceived by participating adolescents. Results: The analyses revealed the repeated appearance of terms associated with violent behaviours, online media, toxic relationships, and victim coercion. However, there was a notable lack of recognition of controlling behaviours as a manifestation of cyber dating violence. Adolescents frequently normalised behaviours such as insisting on knowing a partner’s whereabouts at all times or sharing social media passwords. Conclusions: The findings suggest a significant gap in adolescents’ understanding of what constitutes cyber dating violence. In contrast to direct aggression, which is easily identified, controlling behaviours are often normalised, indicating a need for educational and preventive measures to address this issue. By improving adolescents’ understanding of controlling behaviours as a form of cyber dating violence, preventive efforts can be more effectively tailored to address and mitigate this problem. To prevent the normalisation of certain behaviours indicative of cyber dating violence, early education is recommended in areas such as healthy relationships, communication skills, respect for privacy, and recognition of signs of excessive control.

BIOM-30. NOVEL HISTOLOGIC AND MOLECULAR BIOMARKERS FOR OLIGODENDROGLIOMA

Abstract Oligodendrogliomas are progressive, infiltrative gliomas. While new molecular criteria were introduced in 2016 for diagnosing oligodendroglioma, the grading criteria remains largely unchanged. We evaluated histologic and molecular features for new biomarkers of aggressive behavior, using a cohort of 184 specimens diagnosed as low grade oligodendroglioma within the Duke Brain Tumor Center Biorepository. To identify tumors meeting current molecular criteria for oligodendroglioma, IDH1/2 status was confirmed by whole exome sequencing and 1p/19q codeletion confirmed using inferred copy number variation. First, we assessed for a histologic feature called hypercellular nodules (HCN), which are foci of increased cellularity in regions of otherwise lower cellularity tumor. In patients with newly diagnosed grade 2 oligodendroglioma, 16% (15/92) had HCN. While the median overall survival (OS) was 24.1 years without HCN, the median OS with HCN was only 13.6 years (p<0.02, Mantel-Cox test). We then assessed for homozygous deletion of CDKN2A. Cases with potential CDKN2A homozygous deletion by inferred copy number variation were validated by fluorescence in situ hybridization. In patients with newly diagnosed grade 2 oligodendroglioma, 2% had homozygous CDKN2A deletion (2/107). While the median OS with intact or hemizygous CDKN2A loss was 21 years, it decreased to 3.8 years with homozygous CDKN2A deletion (p<0.0001, Mantel-Cox test), although interpretation is limited given the rarity of homozygous CDKN2A loss at initial diagnosis. For recurrent grade 2 oligodendroglioma, 6% had homozygous deletion (5/77). While the median OS with intact or hemizygous CDKN2A loss in recurrent oligodendroglioma was 25.4 years, it decreased to 6.7 years with homozygous CDKN2A deletion (p< 0.04, Mantel-Cox test). These studies provide support for using HCN and CDKN2A homozygous deletion as grading criteria for oligodendroglioma. Further studies are ongoing, including covariate analysis, expanded cohorts, and ongoing analysis of whole exome sequencing data to identify additional genetic alterations correlated with overall survival.

Capturing Competence: The Design, Evaluation, and Implementation of a Video‐Based Instrument for Assessing Verbal Aggression Management Competence

ABSTRACTComplex competences, such as managing students' aggressive behaviour, are challenging to develop during teacher training. Recently, video‐based simulations have been considered promising, yet suitable assessment instruments are limitedly available. This paper reports on the design and evaluation of a video‐based assessment tool tailored to measure preservice teachers' verbal aggression management competence (V‐AMC) development. Design considerations for video‐based activities served as a framework, resulting in an assessment instrument consisting of video vignettes mirroring classroom situations concerning verbal aggressive behaviour, questions prompting situation‐specific skills development, and a coding scheme to analyse preservice teachers' answers. Furthermore, this paper discusses the results of the instrument's implementation on preservice teachers' (n = 182) V‐AMC. Findings support the appropriateness of the instrument in assessing V‐AMC levels and mapping the impact of a simulation‐based intervention. The data partially confirm the intervention's contribution to V‐AMC development. Implications for future interventions targeting competence development are discussed.

Malignant Esophageal Glomus Tumor With CARMN::NOTCH2 Fusion: An Additional Tumor Underscoring the Frequent Tendency of Esophageal Glomus Tumors for Aggressive Behavior.

Malignant glomus tumors are rare tumors of pericytic origin with a propensity to develop in the upper gastrointestinal tract. Hereby we demonstrate a tumor of a 20-year-old man, who presented with dysphagia and an exophytic esophageal mass. Histologic examination of the resected mass revealed a multinodular tumor in the esophageal wall composed of epithelioid cells showing nesting and monomorphic atypia, staghorn vessels and scanty stroma. Immunohistochemically, the neoplastic cells were positive for SMA, and H-caldesmon, while desmin was negative. Collagen IV and laminin decorated a dense intercellular basal membrane meshwork. RNA-sequencing using TruSight RNA Pan-Cancer Panel revealed a CARMN::NOTCH2 fusion, that is a recurrent, frequently described and so far specific genetic alteration in glomus tumors. In spite of the adjuvant chemotherapy regimens, the patient died of disseminated metastatic disease 2 years after the diagnosis. Our patient presentation and the previous reports in the literature highlight the frequently aggressive behavior of glomus tumors arising in the esophagus.

Assessment of Risk for Immune and Psychiatric Disorders Using qPCR-Based Monitoring of the nan Gene in Gut Microbiota: A Non-invasive Approach

Aim: This study aimed to establish a non-invasive, rapid, and cost-effective method to assess the potential risk of immune and psychiatric disorders by quantifying nan gene levels in gut microbiota, specifically focusing on Ruminococcus gnavus and other Lachnospiraceae species associated with mucin degradation. Methodology: We first designed a primer set targeting the consensus sequence of the nanA gene, which is highly conserved within nan gene clusters. To validate this primer set, we performed Next-Generation Sequencing (NGS) on the PCR-amplified fragments. To explore the association between nan levels and immune or psychiatric disorders, we conducted qPCR to quantify nan levels in the intestines, analyzing intestinal DNA from both allergy-induced mice with or without fructan treatment, and dogs with or without aggressive behavior. Additionally, to assess whether nan levels reflect the clinical status of immune disorders, fecal samples were collected from 45 patients with ulcerative colitis (UC) and analyzed for nan levels. Results: NGS analysis of DNA fragments amplified from various intestinal samples using the nan primer set confirmed the presence of nanA sequences from R. gnavus and other members of the Lachnospiraceae family, including Blautia and Dorea species. The qPCR quantification of nan levels using this primer set revealed that allergy-induced mice treated with fructans, which are known to be associated with lower allergy scores compared to untreated mice, exhibited significantly reduced nan levels. Additionally, the nan levels of aggressive dogs were substantially higher than those of non-aggressive dogs. Notably, nan levels were also substantially elevated in patients with UC in comparison to the healthy control individuals. Conclusion: qPCR-based measurement of nan levels in gut microbiota shows potential for selectively detecting pathogenic nan-harboring strains and may reflect the clinical status of immune and psychiatric disorders. This approach could provide a non-invasive, rapid, and cost-effective method for assessing the risk of these disorders.

Ultimate Fighting Crab: Agonistic Behaviour, Dominance, and Recognition in the Edible Crab, Cancer pagurus (L.)

Edible crabs (Cancer pagurus) are an economically important species for Scottish inshore fisheries, with an estimated annual landing value of GBP 16 million (2023). Research into the behaviour, particularly agonistic behaviour, of this species is currently lacking. This paper aims to investigate behaviour, social interactions, potential hierarchies, and the impact of claw size on the outcomes of agonistic interactions of male C. pagurus through behavioural trials and retrials. Crabs were semi-randomly assigned to pairs (based on allocated condition index rating) and introduced to one another in trial tanks. Each pair underwent two trials, 24 h apart (the “trial” and “retrial”). Analyses of video records of agonistic bouts revealed that 77% of retrials were won by the initial victors, with a significant decrease in fight time between trials and retrials. Fight time was not correlated with weapon size (claw length, height, and depth). There were no differences in weapon size of winners and losers of bouts. Winners exhibited a significantly higher frequency of aggressive and dominant behaviours (approach, aggressive contact, threat displays, and mounting), and losers exhibited higher frequencies of submissive behaviours (withdrawal, retracting limbs, and remaining motionless). These results suggest that individual behaviour may play more of a role in dominance than size or other morphometric characteristics.

The Influence of Emotional Maturity on Aggressive Behavior in Junior High School Students with School Climate as a Moderator Variable

Aggressive behavior is a common problem among both the general public and students. It is disliked in society because it creates a sense of insecurity and has harmful consequences, ranging from physical injury to psychological damage. This study examines the influence of emotional maturity on aggressive behavior with school climate as a moderating variable. A total of 87 students from Bhakti Loa Janan Junior High School, consisting of grades 7, 8, and 9, participated in this study. The study used three valid and reliable measuring instruments, consisting of an aggression scale, school climate, and emotional maturity. The results of the study showed that school climate was not proven to be a moderating variable between emotional maturity and student aggression with a significant value (p) of 0.168. Keywords: emotional maturity, aggressive behavior, school climate

Relationship between Exposure to Violent Content on the Media and Aggressive Behaviour among Adolescent Learners in Western Region, Kenya

This study explores the relationship between exposure to violent media content and aggressive behavior among adolescent secondary school students in Kakamega, Kisii, and Siaya Counties, Kenya. These counties have relatively high media technology access, particularly mobile phones and radio, which exceed national averages. They also report notable incidences of student aggression, with Siaya and Kisii counties showing high rates of student unrest. Grounded in Albert Bandura’s Social Learning Theory, the study utilized a mixed-method approach, adopting descriptive survey and correlational research designs. A sample of 417 students was drawn from a population of 190,555, selected using stratified random sampling method. Data collection involved questionnaires, content analysis, and the Adolescent Peer Relations Instrument (APRI). A pilot study conducted in Vihiga County confirmed the reliability of the instruments, with a Cronbach's Alpha coefficient of 0.87. The study revealed a significant correlation between aggression and exposure to violent content on television (r = 0.211, p = 0.000), with television identified as the most substantial contributor to aggression (B = 0.218, p = 0.001). Notably, 46% of adolescents in Kakamega, Kisii, and Siaya reported constant exposure to violent media, while 11% frequently imitated the violence observed, primarily on mobile phones. Additionally, 9% of respondents justified the violence they encountered, and 56.5% exhibited mild violent tendencies. The study recommends stricter enforcement of media violence regulations, including enhanced penalties and parental guidance ratings, alongside the introduction of media literacy programs in schools. Additionally, it calls for limiting unsupervised internet access for minors, particularly in public cybercafes, to mitigate the negative influence of violent media content.

Effects of anger and trigger identity on triggered displaced aggression among college students: based on the “kicking the barking dog effect”

BackgroundDrawing on the “kicking the barking dog effect”, this study investigated the individual and group-level mechanisms underlying triggered displaced aggression (TDA).MethodsThree experiments were conducted to investigate the effects of anger and hostile attribution on TDA, examining these factors at both the individual and group levels. The preliminary experiment investigated how emotions affect subsequent cognition at an individual level with the aim of understanding the underlying generative process of TDA. Experiment 1 explored the relationship between anger, hostile attribution, and subsequent aggressive behaviors at an individual level, while also discussing the underlying generative process of TDA. Experiment 2 investigated the relationship between anger, hostile attribution, and subsequent aggressive behaviors from a group-level perspective, while also delving into the impact of trigger identity on the underlying generative process of TDA.ResultsWhen individuals were exposed to both a provocation and a subsequent triggering situation, they demonstrated stronger hostile attribution and displayed more aggressive behavior directed toward the trigger. This study found that hostile attribution played a complete mediating role in the influence of anger on TDA, while the triggering situation itself had a moderating role. In the presence of triggering situations, individuals exhibited stronger hostile attribution as their level of anger increased. In the absence of a triggering situation, changes in anger level did not have a significant effect. In the presence of a triggering situation, the identity of the trigger played a moderating role in the process of “anger → hostile attribution → TDA”. When the trigger belonged to an in-group, individuals exhibited stronger hostile attribution toward the out-group and subsequently displayed greater TDA.ConclusionThese findings expand the applicability of the “kicking the barking dog effect” and offer suggestions for controlling the escalation of intergroup conflicts.

The aporetic dialogues of Modena on gender differences: Is it all about testosterone? EPISODE I: CRIME.

This is the first episode of a series of four discussions on the differences between males and females, in apparently non-andrological fields. You will read the transcript of discussions that actually took place at the Endocrinology Unit in Modena, Italy, in the form of the aporetic dialogues of ancient Greece. In this episode, the role of testosterone in gender differences in criminal behavior will be explored. The discussants were divided into two groups: group 1, which supports the thesis of a predominant role of testosterone, and group 2, which opposes it. The first group affirmed that both endogenous testosterone and anabolic-androgenic steroids could trigger aggressive and criminal behavior, regardless of predisposition to psychiatric disease or sociocultural background. The second group asserted the multifactorial genesis of aggressive and criminal behavior, citing other hormonal and non-hormonal factors, such as neurotransmitters, cortisol, and sociological and psychological aspects. In the end, a forensic physician, acting as a referee, tried to resolve the aporia: are the two theories equivalent or one is superior?

How 808 bass affect people's mental performance

This article delves into the "rage music" trend led by Trippie Redd through his song "Miss the Rage" in 2021, particularly focusing on the psychological implications of the pivotal 808 bass drum within this genre. Originating from the Roland TR-808 drum machine, the 808 bass, with its deep and prolonged tone, has become an iconic element in hip-hop music, eliciting potent psychological and physiological responses from listeners. Research indicates that the 808 bass not only stimulates emotional arousal and excitement due to its unique sonic characteristics but may also trigger aggressive behavior as a result of its long-term association with provocative content. While this connection necessitates substantial repetition to establish, the widespread dissemination of hip-hop music has significantly manifested the provocative effect of the 808 bass within specific demographics. Furthermore, the high volume and multimodal perception of the 808 bass during mental performance contribute to the aggressive behavior of audiences. Nevertheless, the study acknowledges that the direct link between the 808 bass and mental performance requires further investigation, taking into account factors such as cultural backgrounds and listening environments.

The Impact of Violent Media on Aggressive Behavior

In contemporary society, advancements in technology and the proliferation of online social media have provided numerous channels for accessing video and gaming content. Despite these developments, the depiction of violence in videos and games remains a contentious issue due to its potential aggressive impact on users. The relationship between exposure to violent media and subsequent aggressive behavior is multifaceted, involving both internal factors (such as perceived aggression, trait anger, violent emotions, and implicit aggression) and external factors (including cultural differences, age of exposure, proximity to real-life violence, domestic violence, and life setbacks). Internal factors significantly influence how individuals process and react to violent media, potentially reinforcing aggressive tendencies. External factors further moderate these effects, with cultural context and early exposure playing crucial roles in shaping long-term behavior. To address the aggression stemming from violent media, a multi-faceted approach is necessary. This includes monitoring and regulating young individuals' media consumption, implementing media literacy programs, providing robust support systems for those facing domestic violence or significant life setbacks, and therapeutic interventions such as cognitive-behavioral therapy. Moreover, promoting cooperative gaming environments and culturally tailored interventions can enhance the effectiveness of these strategies. Ultimately, a comprehensive understanding of the interplay between internal predispositions and external influences is crucial for mitigating the negative societal impacts of violent media.

TERT Gene Mutation in Gliomas Cross-Linked With (NTRK, PDL1, ALK, IDH, P53, EGFR, HER2): A Integrative Review TERT Gene Mutation in Gliomas.

Recent advancements in glioma treatment are largely driven by the identification of genetic alterations, which enhance diagnostic precision and prognostic assessments, and unveil potential therapeutic targets. TERT promoter mutations, in particular, are associated with a poorer prognosis and aggressive clinical behavior. This study explores the genetic interplay between TERT and other genes (ntrk, pdl1, alk, idh, p53, egfr, her2) in brain tumors through an integrative literature review. This method synthesizes evidence from selected articles spanning 2014 to 2023. The review identified 65 articles based on defined inclusion criteria, out of which 14 were analyzed in depth. Findings reveal that TERT, TP53, and IDH1 are the most frequently mutated genes in gliomas. The prognosis of glioma patients can be refined through the combined analysis of IDH and TERT mutations. Additionally, PD-L1 expression levels are associated with prognosis and may influence treatment responses, particularly, in immunotherapy. The study underscores the importance of molecular diagnostics, such as Next-Generation Sequencing (NGS), in detecting key genetic mutations. These advancements have paved the way for new therapeutic strategies and better patient outcomes. The findings highlight the crucial role of genetic markers in glioma treatment and prognosis, advocating for continued research to enhance clinical applications and patient care. The use of NGS is indispensable in identifying biomarkers associated with mutations in the TERT gene.

Histone H3K9 Methylation Is Differentially Modified in Odontogenic Cyst and Tumors.

Histone modification in odontogenic lesions is mostly unexplored. Trimethylation of histone H3 at lysine residue 9 (H3K9Me3) has been studied in various pathologic conditions and showed biological significance promising for future therapeutic application. This study aimed to investigate the level and clinical relevance of the H3K9Me3 histone modification in odontogenic cysts and tumors. A total of 105 cases of odontogenic lesions, comprising 30 odontogenic keratocysts (OKCs), 30 adenomatoid odontogenic tumors (AOTs), 30 ameloblastomas, and 15 dental follicles, were included in the study. The paraffin-embedded tissues were immunohistochemically stained for H3K9Me3. Both the intensity and the distribution of staining were evaluated and calculated as H-score. The correlation between the H3K9Me3 expression and the clinical characteristics of each lesion was evaluated. The Kruskal-Wallis test followed by Bonferroni's correction was performed to assess the differences in H-score among groups. In addition, Pearson's chi-squared test or Mann-Whitney U test was used to analyze potential factors that could affect protein expression. The reduced enamel epithelium of the dental follicle showed uniformly strong H3K9Me3 expression. All odontogenic cysts and tumors examined demonstrated a significantly reduced H3K9Me3 level compared with dental follicles. The AOT showed the lowest H3K9Me3 level, followed by OKC and ameloblastoma. Its immunoreactivity was mainly localized in the basal and parabasal cells of OKC and the whorled/duct-like structures of AOT. Ameloblastoma exhibited marked variation in the H3K9Me3 level among cases. Notably, the upregulated H3K9Me3 was related to multilocularity of OKC and ameloblastoma. Histone H3K9 methylation is differentially expressed in odontogenic cysts and tumors. This epigenetic modification may contribute to the pathogenesis and aggressive behavior of odontogenic lesions.

Love is a Losing Game: Aggression as a Function of Attachment and Gender Among Cape Verdean Young Adults

ABSTRACT This study aimed to investigate the association between inhibition of exploration and individuality with both mother and father figure, ambivalent attachment style, and aggression dimensions (anger, hostility, physical, and verbal aggression) among Cape Verdean young adults in a romantic relationship. Moreover, the study proposed aggression dimensions as a mediator between the relationship of the aforementioned variables. The study followed a cross-sectional design performed between May and December 2023 among 123 Cape Verdean young adults aged 18 to 35 who reported to have been in an intimate relationship in the last 6 months. Hayes’ PROCESS macro for mediation analysis in SPSS was used to test the hypothesized model. The results suggested that anger and hostility had a significant relationship with inhibition of exploration and individuality, with the mother figure and ambivalent attachment style. Women manifested higher levels of anger and hostility, as well as ambivalent attachment in comparison to men. Additionally, hostility played a significant mediator role between the relationship of the aforementioned variables. The present study adds to the literature by addressing differences in genders’ manifestation of aggressive behaviors in romantic relationships and provides a critical perspective of existing literature with recommendations and limitations of the field.