Aggregate-associated Organic Carbon Research Articles

Vegetation restoration changed the soil aggregate stability and aggregate carbon stabilization pathway according to δ13C signatures

Vegetation restoration can increase soil organic carbon (SOC) sequestration through the physical protection of soil aggregates. However, the soil aggregate stability and C flow pathway associated with long-term plantation restoration have not yet been fully characterized. Here, we conducted a study on Robinia pseudoacacia plantations at different recovery stages, studied the distribution and stability of aggregates, analysed the aggregate-associated organic carbon (OC) content and δ13C value, and quantified the aggregate C flow pathway. The results revealed that vegetation restoration increased the proportion of large macroaggregates (LMAs) and decreased the proportion of small macroaggregates (SMAs), with no changes observed in the proportion of microaggregates (MIAs) or silt + clay (SC) at 0–20 cm. The indices of aggregate stability, namely, the mean weight diameter (MWD), geometric mean diameter (GMD) and structural stability index (SSI), increased under vegetation restoration at 0–20 cm, with maximum values of 3.83 mm, 2.88 mm, and 2.00 %, respectively, at 35 years of age (35Y). The OC content of the LMAs increased from 10.96 to 21.64 g kg−1 and from 7.27 to 10.05 g kg−1 in the 0–20 cm and 20–40 cm layers, respectively. LMAs and SMAs had the greatest contributions to SOC accumulation in the 0–20 cm and 20–40 cm layers, respectively. The δ13C value increased with decreasing aggregate size. The C flow pathway was from macroaggregates to MIAs or SC. Compared with abandoned farmland, vegetation restoration decreased the aggregate C flow intensity in the 0–20 cm layer. The soil aggregate stability and aggregate-associated OC content decreased with increasing soil depth, but the soil δ13C value exhibited the opposite trend. Vegetation restoration regulated soil aggregate stability by influencing the fine root biomass (FRB) and SOC content. In summary, our analysis offers a valuable reference for the controlling effect of aggregation on C stability influenced by vegetation restoration.

Decade-long effects of integrated farming systems on soil aggregation and carbon dynamics in sub-tropical Eastern Indo-Gangetic plains

Integrated farming system (IFS) aims to diversify the agricultural landscapes by incorporating different components to meet the multifarious needs of the burgeoning population. The present study was undertaken to understand the impact of different cropping systems on soil organic carbon (SOC) stock, aggregate distribution, and aggregate associated organic carbon (AAOC) in 2-IFS models of varying sizes (0.4 and 0.8 ha) established during 2008–2009. After 10 years of the study, the fodder system registered the greatest TOC and carbon stocks across IFS models, with surface soil (0–15 cm) accumulating 17 and 13% higher TOC and C stock, respectively, in 0.4 and 0.8 ha models. In 0–15 cm, macroaggregates (Ma) represented the highest proportion (75–76%) in both models. Among cropping systems, the fodder system recorded the highest large macroaggregates in both IFS models. Within 0–30 cm depth, small macroaggregates are mostly found in the perennial system (fodder, guava+turmeric, and lemon intercropping system), indicating the potential to improve the aggregate stability over the seasonal (shorter duration) system. In general, micro aggregate (Mi) fraction was pre-dominant in sub-surface soil (17.35%). The maximum AAOC was found in Ma compared to Mi fractions, with approximately 67 and 63% of total carbon associated with Ma in 0.4 and 0.8 ha IFS models, respectively. Interestingly, the 0.8 ha IFS model had higher TOC (~11%) and carbon stock (~12%) than the 0.4 ha model, but AAOC did not show a similar result, indicating the influence of cropping systems on AAOC. The study indicated that the fodder-based production system had better performance in terms of soil physical health and increased aggregate stability and content of soil carbon. This is indicative of the advantages of perennial-based systems over seasonal- or annual-based cropping systems for soil sustainability in Eastern Indo-Gangetic Plains.

Microaggregates regulate the soil organic carbon sequestration and carbon flow of windproof sand fixation forests in desert ecosystems

Afforestation improves the soil organic carbon (SOC) sequestration by affecting aggregates formation. However, the impact of the establishment and development of windproof sand fixation forests on soil carbon (C) flow and sequestration in desert regions are largely unclear. The space-for-time method was used to elucidate changes in bulk soil, aggregate-associated organic carbon (OC) content, stock, and C flow with afforestation years and the main influencing factors. We sampled from 0–20 cm, 20–60 cm and 60–100 cm in natural desert (CK) and windproof sand fixation forests after 3, 7, and 10 years of afforestation. The direction of C flow within the aggregates was quantified using the δ13C natural abundance method. Results showed that compared to the CK, afforestation increased in the bulk soil OC stock in the 0 − 100 cm by 2.97, 5.34, and 1.67 Mg·ha−1 at 3, 7, and 10 years of afforestation, respectively. The bulk soil OC content decreased gradually, but the stock increased with soil depth. Furthermore, C sequestration in this region mainly relied on the microaggregates OC. Microaggregates acted as both a “source” and a “sink” in desert ecosystems. During the 7 years of afforestation, the δ13C in the aggregates decreased with increasing soil depth, indicating older C in the subsoil. The variations in bulk soil OC stock were mainly regulated by ammonium nitrogen, nitrate nitrogen, and total nitrogen. Therefore, the results suggest that 7 years of afforestation may be the optimal choice among the three afforestation years compared for C sequestration.

Precipitation patterns impact soil aggregates and organic carbon of an alpine wetland on the Qinghai-Tibetan Plateau

Global climate change is predicted to influence both precipitation amount and frequency in many regions. However, little is known about how precipitation changes affect soil aggregates and soil organic carbon (SOC) in alpine wetlands. Plots of an alpine wetland on the Qinghai-Tibetan Plateau were subjected to two precipitation frequencies (high vs. low, i.e., 1 and 3 times of nature rainfall intervals) crossed with two precipitation amounts (high vs. low, i.e., 100 % and 70 % of the amount of natural rainfall). Two years later, we investigated changes of soil aggregates (silt + clay, < 53 μm; microaggregates, 53∼250 μm; and macroaggregates, >250 μm) and SOC in three soil depths, i.e., 0–15 cm, 15–30 cm and 30–50 cm. Averaged across the three soil depths, the content of SOC and dissolved organic carbon (DOC) in the simulated natural precipitation (high amount, high frequency treatment) were 127.22 g kg−1 and 0.19 g kg−1, respectively. Reducing precipitation amount or frequency led to a 19.6 %-26.2 % increase in SOC, and 28.7 %-44.4 % increase in DOC. Microbial biomass carbon in natural precipitation was 1.33 g kg−1 across the three soil depths, and its response to precipitation varied with soil depths. The fraction of macroaggregates in the 0–15 cm soil depth was 63.5 % in the simulated natural precipitation treatment, and decreased by 18.6 %–21.9 % in response to reduced precipitation amount or frequency. Aggregate fractions in the 15–30 cm soil depth did not respond significantly to the changes of precipitation patterns. Reducing precipitation frequency or amount decreased the contribution of macroaggregate-associated organic carbon to SOC compared to the simulated natural precipitation, but increased the contribution of microaggregate-associated organic carbon, especially in the 0–15 cm soil depth. Structural equation models revealed that SOC was regulated by soil aggregate composition and microbial biomass carbon. The buffering of the surface soil weakened the effects of precipitation changes on aggregates and aggregate-associated organic carbon in the deep soil. These results suggest that reducing precipitation frequency or amount can decrease the fraction of macroaggregates in the surface soil, and weaken the physical protection of macroaggregates for SOC. Thus, changes in precipitation patterns may have a significant impact on SOC storage and stability in alpine wetlands through their influence on soil aggregate composition.

Conservation tillage enhances the sequestration and iron-mediated stabilization of aggregate-associated organic carbon in Mollisols

Conservation tillage practices, which involve minimal or no soil disturbance and crop residue retention, are known to help preserve soil organic carbon (SOC). However, the mechanisms underlying the minerals-mediated chemical and physical stabilization of SOC remain unclear. Here, a long-term field experiment was initiated in 2012 to investigate the effects of tillage managements on the contents and chemical composition of SOC density fractions, iron oxides transformation and aggregate stability in Mollisols. We utilized three treatments: conventional tillage (CT) without crop residue, reduced tillage (RT) and no tillage (NT) with straw mulching. Compared to CT, RT and NT significantly increased SOC content by 13.6 % and 17.9 % in the 0–20 cm soil layer due to an increase in the aromatic compound contents. Furthermore, NT and RT increased the mean weight diameter by 17.7 % and 10.7 %, respectively, indicating increased aggregate stability compared to CT. Additionally, the contents of amorphous iron oxides (Feo) and complex iron oxides (Fep) increased under NT and RT by 10.6–14.4 % and 12.7–41.1 %, respectively, in bulk soil and silt + clay fractions within macroaggregates (>0.25 mm). The contents of Feo and Fep were strongly positively correlated with aggregate stability (p < 0.001, r2 = 0.64), and promote the physical protection of SOC. Both NT and RT enhanced the aromatic-C content and aromatic-C/aliphatic-C ratio by 13.6 %–24.6 % and 16.5 %–38.9 % in macroaggregates compared with CT. Moreover, the aromatic-C/aliphatic-C ratio increased with increasing Feo plus Fep contents (p < 0.001, r2 = 0.73), which led to an increased in the recalcitrant-C proportion, and this shift was benefit for the accumulation of mineral-associated OC. These results indicate that long-term conservation tillage can augment the accessibility of Fe for binding C, possibly by forming organo-Fe complexes, which subsequently improve soil aggregation, and thus promoted the chemical stability and long-term sequestration of SOC in Mollisols of Northeast China.

Effects of Different Land Use Change on Soil Aggregate and Aggregate Associated Organic Carbon: A Meta-Analysis

Effects of Different Land Use Change on Soil Aggregate and Aggregate Associated Organic Carbon: A Meta-Analysis

Different Land-Use Effects on Soil Aggregates and Aggregate-Associated Organic Carbon in Eastern Qinghai–Tibet Plateau

Land use changes modify soil properties, including aggregate structure, and thus, profoundly affect soil quality and health. However, the effects of land use changes originating from alpine grassland on soil aggregates and aggregate-associated organic carbon have received little attention. Soil aggregate fraction, aggregate-associated organic carbon and relevant influencing factors were determined at 0–20, 20–40 cm soil layers for alpine grassland, cropland and abandoned land in the eastern Qinghai–Tibet Plateau (QTP), and their relationships were analyzed by partial least square regression (PLSR). Results showed the following: (1) conversion from alpine grassland to cropland resulted in a significant decline macroaggregate fraction (R0.25), mean weight diameter (MWD), mean weight diameter (GMD), soil organic carbon (SOC), and microaggregate-associated SOC; (2) almost all aggregate stability indexes, SOC, and aggregate-associated SOCs were significantly positively correlated with silt and glomalin, suggesting that the binding of fine particles (silt) with the organic cementing agent (glomalin) was probably a key mechanism of SOC formation and aggregate stability in the studied region; (3) compared with biotic factors such as SOC, glomalin and root biomass, abiotic factors including silt and sand can better predict aggregate stability and SOC fraction using the PLSR model. The above results indicated that the conversion of alpine grassland to other land use types in high altitude areas would destroy soil structure and decrease soil organic carbon content, and then reduce soil quality.

Biochar addition promotes soil organic carbon sequestration dominantly contributed by macro-aggregates in agricultural ecosystems of China

Soil aggregates play pivotal roles in soil organic carbon (SOC) preservation and climate change. Biochar has been widely applied in agricultural ecosystems to improve soil physicochemical properties. However, the underlying mechanisms of SOC sequestration by soil aggregation with biochar addition are not well understood at a large scale. Here, we conducted a meta-analysis of 2335 pairwise data from 45 studies to explore how soil aggregation sequestrated SOC after biochar addition in agricultural ecosystems of China. Biochar addition markedly enhanced the proportions of macro-aggregates and aggregate stability, and the production of organic binding agents positively facilitated the formation of macro-aggregates and aggregate stability. Soil aggregate-associated organic carbon (OC) indicated a significantly increasement by biochar addition, which was attributed to direct and indirect inputs of OC from biochar and organic residues, respectively. Biochar stimulated SOC sequestration dominantly contributed by macro-aggregates, and it could be interpreted by a greater improvement in proportions and OC protection of macro-aggregates. Furthermore, the SOC sequestration of soil aggregation with biochar addition was regulated by climate conditions (mean annual temperature and precipitation), biochar attributes (biochar C/N ratio and pH), experimental practices (biochar addition level and duration), and agronomic managements (land type, cropping intensity, fertilization condition, and crop type). Collectively, our synthetic analysis emphasized that biochar promoted the SOC sequestration by improving soil aggregation in agricultural ecosystems of China.

Agricultural land abandonment promotes soil aggregation and aggregate-associated organic carbon accumulation: a global meta-analysis

Agricultural land abandonment promotes soil aggregation and aggregate-associated organic carbon accumulation: a global meta-analysis

Effects of Water-Level Fluctuation on Soil Aggregates and Aggregate-Associated Organic Carbon in the Water-Level Fluctuation Zone of the Three Gorges Reservoir, China

Water-level fluctuation (WLF) can destroy soil aggregates and induce soil organic carbon (SOC) loss, potentially triggering impacts on the concentration of atmospheric carbon dioxide. However, responses of soil aggregate content and aggregate-associated organic carbon to WLF have not been well studied, especially in the water-level fluctuation zone (WLFZ) of the Three Gorges Reservoir (TGR). Therefore, samples from different elevations (145 m, 155 m and 165 m) in the WLFZ of the TGR were collected for experiments. The wet sieving method was used to divide soil into silt and clay (&lt;0.053 mm), micro-aggregate (0.053–0.25 mm) and macro-aggregate (&gt;0.25 mm). The K2Cr2O7-H2SO4 oxidation method was used to measure total SOC content in different soil aggregates. A modified Walkley and Black method was used to measure labile carbon in different soil aggregates. Results showed that macro-aggregate content substantially decreased, while micro-aggregate content remained stable and silt and clay fraction accumulated with a decrease in water-level elevations. Moreover, total SOC content and labile carbon in macro-aggregate were obviously higher than those in the micro-aggregate and the silt and clay fraction. Macro-aggregate contributed the most to SOC sequestration, while micro-aggregate contributed the least, and the contribution of macro-aggregate increased with a decrease in water-level elevations. We concluded that the macro-aggregate was the most active participant in the SOC sequestration process, and preferentially increasing the macro-aggregate content of the lowest water-level elevation was conducive to an improvement in soil carbon sequestration potential and would mitigate climate change.

Soil Aggregate Stability and Organic Carbon Content among Different Forest Types in Temperate Ecosystems in Northeastern China

Soil aggregates play a crucial role in substance and energy cycles in soil systems. The fixation of soil organic carbon (SOC) is closely tied to the safeguarding mechanisms of soil aggregates. Carbon fixation involves the conversion of atmospheric carbon dioxide into organic molecules by autotrophic organisms. Soil aggregates play a significant role in carbon stabilization, allowing for the physical occlusion of SOC. This study focuses on five forest types, Betula platyphylla, Betula dahurica, Quercus mongolica, Larix gmelinii, and mixed forests comprised of Larix gmelinii and Quercus mongolica, in the Heilongjiang Central Station Black-billed Capercaillie National Nature Reserve, northeast of China. This study investigated the soil aggregate stability (SAS) (water sieving) and aggregate-associated organic carbon (AAOC) at different soil depths in five forest types. Our findings demonstrated that fine macro-aggregates (0.25–2 mm) were the main types of soil aggregates among all the forest types. The SAS gradually decreased with increasing soil depth. Notably, broad-leaved forests exhibited relatively high soil stability. The fine macro-aggregates (0.25–2 mm) had the highest AAOC content, and the AAOC level was highest in the topsoil layer. The SAS and AOCC levels of the Betula platyphylla forest and Betula dahurica forest were higher than those of other forest types and were significantly affected by the forest type, soil depth, and soil physicochemical properties. Collectively, our findings reveal the key factors influencing aggregate stability and the variations in soil organic carbon content in different forest types. These observations provide a basis for studying the mechanisms of soil aggregate carbon sequestration, as well as for the sustainable development of forest soil carbon sequestration and emission reduction.

Vegetation restoration improved aggregation stability and aggregated-associated carbon preservation in the karst areas of Guizhou Province, southwest China.

The change in the soil carbon bank is closely related to the carbon dioxide in the atmosphere, and the vegetation litter input can change the soil organic carbon content. However, due to various factors, such as soil type, climate, and plant species, the effects of vegetation restoration on the soil vary. Currently, research on aggregate-associated carbon has focused on single vegetation and soil surface layers, and the changes in soil aggregate stability and carbon sequestration under different vegetation restoration modes and in deeper soil layers remain unclear. Therefore, this study aimed to explore the differences and relationships between stability and the carbon preservation capacity (CPC) under different vegetation restoration modes and to clarify the main influencing factors of aggregate carbon preservation. Grassland (GL), shrubland (SL), woodland (WL), and garden plots (GP) were sampled, and they were compared with farmland (FL) as the control. Soil samples of 0-40 cm were collected. The soil aggregate distribution, aggregate-associated organic carbon concentration, CPC, and stability indicators, including the mean weight diameter (MWD), fractal dimension (D), soil erodibility (K), and geometric mean diameter (GMD), were measured. The results showed that at 0-40 cm, vegetation restoration significantly increased the >2 mm aggregate proportions, aggregate stability, soil organic carbon (SOC) content, CPC, and soil erosion resistance. The >2 mm fractions of the GL and SL were at a significantly greater proportion at 0-40 cm than that of the other vegetation types but the CPC was only significantly different between 0 and 10 cm when compared with the other vegetation types (P<0.05). The >2 mm aggregates showed a significant positive correlation with the CPC, MWD, and GMD (P<0.01), and there was a significant negative correlation with the D and K (P<0.05). The SOC and CPC of all the vegetation types were mainly distributed in the 0.25-2 mm and <0.25 mm aggregate fractions. The MWD, GMD, SOC, and CPC all gradually decreased with increasing soil depth. Overall, the effects of vegetation recovery on soil carbon sequestration and soil stability were related to vegetation type, aggregate particle size, and soil depth, and the GL and SL restoration patterns may be more suitable in this study area. Therefore, to improve the soil quality and the sequestration of organic carbon and reduce soil erosion, the protection of vegetation should be strengthened and the policy of returning farmland to forest should be prioritized.

Substitution of Chemical Fertilizer with Organic Fertilizer Can Affect Soil Labile Organic Carbon Fractions and Garlic Yield by Mediating Soil Aggregate-Associated Organic Carbon

This study aimed to explore the impact paths on soil organic carbon and crop yield of completely or partially substituting chemical N fertilizer with organic fertilizers. A four-year field experiment was conducted and included four treatments: (i) N0, no N fertilization application; (ii) NF, only synthetic N fertilizer application; (iii) 1/2OF, organic fertilizer substituted for 100% of the synthetic N fertilizer, with the total N application amount being equivalent to half that of NF; and (iv) 1/3OF + 2/3NF, organic fertilizer substituted for 1/3 of the synthetic N fertilizer with the total N application amount from organic and synthetic fertilizer being equivalent to that of NF. Soil total organic carbon (TOC), labile organic-carbon fractions (microbial biomass carbon (MBC), dissolved organic carbon (DOC), particulate organic carbon (POC), and easily oxidized organic carbon (EOC)), the carbon pool management index (CPMI), soil aggregated distribution, and water-stable aggregate-associated organic carbon were determined. Structural equation modeling (SEM) was used to clarify the impact paths of TOC and garlic yield changes under different N fertilizer treatments. Results showed that compared with N0 and NF, 1/2OF and 1/3OF + 2/3NF significantly increased TOC contents by 14.1–20.6%. Soil MBC, DOC, and EOC under 1/2OF were significantly higher than under N0, whereas the 1/3OF + 2/3NF treatment had significantly greater POC. The CPMI was improved by organic fertilizer treatment, with 1/2OF treatment being significantly higher than N0 and NF. The proportion of soil aggregate mass with particle sizes &gt;2 mm was significantly greater under N0, while 1/3OF + 2/3NF significantly increased the proportion of particle sizes of 0.5–2 mm. Soil water-stable aggregate-associated organic carbon showed a trend of first increasing and then decreasing, with the largest particle sizes being 1–2 mm. Moreover, organic fertilizer significantly increased soil water-stable aggregate organic carbon compared with N0 and NF. Similarly, the garlic yield increased with organic fertilizer treatment, while 1/3OF + 2/3NF significantly increased the yield by 37.2% and 15.3%, respectively, compared with N0 and NF. Furthermore, SEM analysis indicated that fertilizer regimes could directly affect TOC and labile organic carbon components by affecting aggregate-associated organic carbon. In particular, aggregates with particle sizes of 0.5–2 mm played an important role, indirectly affecting garlic yield and CPMI. These results indicate that organic fertilizer application has the potential to improve soil organic-carbon content and garlic yield; moreover, fully applying organic fertilizer can reduce N fertilizer input while still maintaining an increase in soil organic carbon and crop yield in the short term. However, caution is still needed regarding of the type and quantity of organic fertilizer added in different cropping systems, and with different soil textures.

Natural and regenerated saltmarshes exhibit different bulk soil and aggregate-associated organic and inorganic carbon contents but similar total carbon contents

Saltmarshes are considered to be one of the planet's most efficient carbon sinks. The continued loss of saltmarshes and induced ecological consequences promoted their restoration worldwide. Previous efforts aimed to evaluate the success of restoration in terms of organic carbon accumulation, but inorganic carbon and carbon contents within soil aggregates, which are essential for making a comprehensive assessment of the carbon sink function, were rarely studied. To fill this gap, a range of metrics including bulk and aggregate-associated soil organic and inorganic carbon contents together with the soil's physical, chemical and microbiological parameters were measured to compare natural and a 15-year restoration effort in saltmarsh habitats within the Yellow River Delta region in eastern China. The results showed that regenerated saltmarsh exhibited significantly higher soil organic carbon (SOC) contents but significantly lower soil inorganic carbon contents, resulting in no notable change in total carbon contents between the regenerated and natural saltmarshes. SOC contents within the silt and clay fractions and their contribution to the bulk SOC contents were significantly lower in the regenerated saltmarsh than those in the natural ones (P < 0.05). In regenerated saltmarsh, significantly lower soil microbial biomass and distinct microbial community composition with reduced Gram-negative to Gram-positive bacteria ratios were observed compared to natural saltmarsh. These findings indicate the stability of SOC fraction and soil microbe-mediated carbon biogeochemical processes differed between naturally occurring and artificially regenerated saltmarshes. As interest in blue carbon programs gains global attention, further research on the generation and transformation processes of different carbon fractions during restoration are needed, which can be conducive to elucidating more details in coastal carbon cycling processes.

Impacts of abandoned sloping farmland on soil aggregates and aggregate-associated organic carbon in karst rocky desertification areas.

Vegetation restoration after the abandonment of sloping farmland can effectively promote the sequestration of soil organic carbon (SOC), with soil aggregates playing a pivotal role. However, in abandoned farmlands in karst regions with varying degrees of rocky desertification, the relationship between soil aggregates, aggregate-associated organic carbon (AAOC), and total SOC content remains unclear. Taking abandoned sloping farmlands (5years, 10years, and 15years) with different levels of rocky desertification (no rocky desertification, potential rocky desertification, slight rocky desertification, and moderate rocky desertification) in a typical karst area as research objects, this study investigated the dynamic characteristics of the particle size distribution of soil aggregates, total SOC, and AAOC. The results indicated that total SOC content in the 0-20cm soil layer increased after abandonment in all levels of rocky desertification, peaking after 15years. The abandoned sloping farmland with moderate desertification showed the best recovery effect. Post-abandonment vegetation restoration increased the content of 5-10mm soil aggregates, but decreased those of 1-2mm and < 0.25mm aggregates. Particularly for 5-10mm aggregates, the contribution of AAOC to total SOC significantly increased over time. Moreover, a strong correlation was observed between >1mm aggregates and total SOC (p < 0.05). The increase in total SOC was primarily driven by the growth of AAOC in 5-10mm aggregates. In general, vegetation restoration is an effective approach for enhancing total SOC content in abandoned sloping farmland with varying degrees of rocky desertification.

Changes in aggregate-associated carbon and microbial respiration affected by aggregate size, soil depth, and altitude in a forest soil

This study investigates the variations of aggregate-associated organic carbon (OC) and microbial respiration (MR) with respect to aggregate size, soil depth, and land altitude in a forest soil. The research was conducted in Arasbaran forest, East Azerbaijan province, Iran. Four sites were selected at different altitudes: 0–600 m, 600–1200 m, 1200–1800 m, and 1800–2400 m above sea level (a.s.l.), along the north-facing slope of the forest region. Soil samples were collected at five depths: 0–20 cm, 20–40 cm, 40–60 cm, 60–80 cm, and 80–100 cm, with three replications for each depth. The soil samples were then fractioned into four aggregate size classes: <0.053 mm, 0.053–0.25 mm, 0.25–2 mm, and >2 mm. Both soil samples and aggregate fractions were analyzed for OC content and MR. The results revealed that OC content and MR in both soil samples and aggregate fractions increased with altitude and decreased with soil depth. Moreover, aggregate-associated OC increased with aggregate size. Specifically, as soil aggregate sizes increased from <0.053 mm to >2 mm at different altitudes, the mean occluded OC content of aggregates increased by approximately four times. Similarly, the mean aggregate-associated MR intensified eight times with the increase in aggregate size. A significant positive correlation was also observed between OC and MR rates of all aggregates. Furthermore, aggregates larger than 2 mm exhibited the highest contribution to the total soil OC storage (47.47–57.22%) and CO2 production rate (20–21%). These findings underscore the critical role of large macro-aggregates (>2 mm) in soil carbon storage and microbial activity, particularly in surface soils at higher altitudes.

Soil aggregate-associated organic carbon mineralization and its driving factors in rhizosphere soil

Understanding the determinants of soil carbon mineralization at both the aggregate and rhizosphere levels is crucial for providing precise feedback on climate change. However, the specific patterns and relative contributions of rhizosphere effects on carbon mineralization at the aggregate scale remain unclear. To address this, we conducted an incubation experiment to examine the impact of warming on soil carbon mineralization. We also assessed variations in microbial activities and soil properties across different aggregates in both rhizosphere and non-rhizosphere soils, while exploring how the rhizosphere effects modulated the response of soil respiration to warming. Our findings revealed that rhizosphere soil provides a favorable environment for microbial activities through increased nutrient input and aggregate stability, leading to significant increases in microbial biomass and enzyme activity, thus promoting soil carbon mineralization. Moreover, the spatial heterogeneity of soil aggregates contributes to the differentiation and diversity of microbial community distribution, which further enhances the diversification of carbon mineralization at the aggregate level. Notably, macroaggregates play a significant role in soil carbon flux, making a substantial contribution to carbon turnover in the ecosystem. The partial least squares (PLS) path analysis indicated that the rhizosphere positively increased the contributions of soil properties and microbial variables to the overall amount of soil cumulative mineralization, with soil nutrients playing a crucial role among these factors. These findings highlight the complex regulatory role of substrate quality in soil carbon mineralization dynamics at the aggregate scale, underscoring its far-reaching implications for accurately predicting the feedback to climate change in soil carbon mineralization.

Organic matter mineralization, aggregation, and aggregate-associated organic carbon in saline soil of arid region of Tunisia: a laboratory incubation

Organic matter mineralization, aggregation, and aggregate-associated organic carbon in saline soil of arid region of Tunisia: a laboratory incubation

Nitrogen-fixing tree species modulate species richness effects on soil aggregate-associated organic carbon fractions

Soil aggregation is an important mechanism shaping soil organic carbon (SOC) fractions, but the effects of tree species richness on soil aggregate-associated organic carbon fractions are poorly understood. In this study, a manipulative experiment was conducted to examine whether and how tree species richness (1, 2, 4 and 6) together with or without the presence of nitrogen (N)-fixing tree species affect soil aggregate-associated organic carbon. Soil aggregates were classified into four fractions: large macroaggregates (>2 mm), small macroaggregates (0.25–2 mm), microaggregates (0.053–0.25 mm), and silt and clay (<0.053 mm). And then large and small macroaggregates were further separated into coarse (c-iPOC), fine intra-aggregate particulate organic carbon (f-iPOC) and mineral associated organic carbon (MAOC), and microaggregates were divided into f-iPOC and MAOC. We observed that the effects of tree species richness on soil aggregate-associated organic carbon fractions were depended on the presence or absence of N-fixing tree species. In the presence of N-fixing tree species, organic carbon contents of small macroaggregate, microaggregate and silt and clay fractions were increased across the gradient of tree species richness (+10.4%, +31.3% and + 26.7% from monocultures to 6-species mixture), and the similar responses occurred for c-iPOC within small macroaggregates (+42.9%) and f-iPOC within microaggregates (+50.0%). In the absence of N-fixing tree species, however, only f-iPOC within large macroaggregates (+60.0%) had a positive linear relationship with tree species richness. Structural equation modeling was then used to dissect the mechanism underlying the positive effects of tree species richness on the aggregate-associated organic carbon fractions, indicating either direct or indirect effects by increasing root length density. Our study highlights the important roles of N-fixing tree species in shaping tree species richness effects on soil aggregate-associated organic carbon fractions, and therefore, N-fixing tree species should be preferentially considered in mixed-species plantations to enhance long-term SOC stabilization.

The increased soil aggregate stability and aggregate-associated carbon by farmland use change in a karst region of Southwest China

Land use change can significantly alter the distribution of different soil aggregates, thereby influencing aggregate stability and aggregate-associated organic carbon (OC). However, there is minimal research on the variations in the distribution of soil aggregates, aggregate stability, and aggregate-associated OC following land use change from farmland to afforested land in the karst regions of Southwest China. Undisturbed soil samples were collected from farmland (FL) and three afforestation patterns (bamboo forest: BA; landscape tree planting: LAT; and orange orchards: ORO) converted from farmland in a karst region of Southwest China. These four land uses have similar geographical characteristics (e.g., elevation and slope aspects) and previous framing practices. The distribution of macroaggregate (>0.25 mm), microaggregate (0.053–0.25 mm) and silt + clay fractions (<0.053 mm) and the aggregate-associated OC contents under the four land uses were measured at the 0–10 cm and 10–20 cm depths. The results showed that afforestation converted from FL significantly increased the macroaggregate and microaggregate contents, geometric mean diameter (GMD), and structural stability index (SSI) but decreased the silt + clay fraction content. Compared with FL, the values at the 0–20 cm depth under BA, LAT and ORO increased by 108.79%, 34.89% and 36.56%, respectively, for GMD and by 239.84%, 94.05%, and 123.41%, respectively, for SSI. Land use change and soil aggregate size had obvious influences on soil organic carbon (SOC) content. The SOC content in the bulk soil, macroaggregate, microaggregate, and silt + clay fraction at the 0–20 cm depth after afforestation increased by 26.83%, 45.57%, 27.14%, and 13.80%, respectively, compared to FL. The contributions of macro- and microaggregates to the increase in SOC content in bulk soil were 146% and 78%, respectively, while the silt + clay fractions had a negative effect with a value of 125% after afforestation. The GMD and SSI were positively correlated with the macroaggregate content and SOC content in the bulk soil and different soil aggregates. Although the increase in SOC content in bulk soil and soil aggregates under BA and ORO was significantly higher than that for LAT, the soil aggregate stability under ORO was lower than that under BA. These findings suggest that afforestation increases aggregate ability, SOC content in bulk soil and aggregate-associated OC and that BA might be the optimum choice among the three afforested lands compared for soil sustainability from the perspective of improving carbon sequestration and soil erosion.