Agglutination Method Research Articles

Clinical application of ABO blood typing.

The ABO blood group is closely related to clinical blood transfusion, transplantation, and neonatal hemolytic disease. It is also the most clinically significant blood group system in clinical blood transfusion. The purpose of this paper is to review and analyze the clinical application of the ABO blood group. The most common ABO blood group typing methods in clinical laboratories are hemagglutination test and microcolumn gel test, while genotype detection is mainly adopted in clinical identification of suspicious blood types. However, in some cases, the expression variation or absence of blood type antigens or antibodies, experimental techniques, physiology, disease, and other factors affect the accurate determination of blood types, which may lead to serious transfusion reactions. The mistakes could be reduced or even eliminated by strengthening training, selecting reasonable identification methods, and optimizing processes, thereby improving the overall identification level of the ABO blood group. ABO blood groups are also correlated with many diseases, such as COVID-19 and malignant tumors. Rh blood groups are determined by the RHD and RHCE homologous genes on chromosome 1 and are classified as Rh negative or positive according to the D antigen., the agglutination method is often used in clinical settings, while genetic and sequencing methods are often used in scientific research. Accurate ABO blood typing is a critical requirement for the safety and effectiveness of blood transfusion in clinical practice. Most studies were designed for investigating rare Rh blood group family, and there is a lack of research on the relationship between Rh blood groups and common diseases.

Epidemiology and antimicrobial resistance profile of invasive non-typhoidal Salmonella from the Philippines Antimicrobial Resistance Surveillance Program, 2014-2018.

The epidemiology of invasive non-typhoidal Salmonella (iNTS) in the Philippines is not well elaborated. The present study describes the serotype distribution and antimicrobial susceptibility patterns of iNTS in the Philippines from 2014 to 2018. Invasive NTS isolates were collected through the Department of Health's Antimicrobial Resistance Surveillance Program (ARSP). The identification of the isolates was confirmed using automated (Vitek®, bioMérieux, Marcy l'Étoile, France) and conventional methods. The isolates were serotyped using the slide agglutination method, and susceptibility testing was performed using Clinical and Laboratory Standards Institute guidelines. Demographic data were collected from the ARSP database. There were 138 isolates collected from human invasive specimens with 97.8% from blood samples. The most common serotypes were Salmonella Enteritidis (n=84, 60.9%) and Salmonella Typhimurium (n=18, 13.0%). Most of the isolates were from males (n=88, 63.8%) and from the 0-5-year age group (n=61, 44.2%). The proportions of iNTS isolates resistant to first-line antibiotics were as follows: ampicillin (23.2%), chloramphenicol (9.6%), ciprofloxacin (8.7%), ceftriaxone (2.2%) and trimethoprim-sulfamethoxazole (8.8%). The proportion of isolates with multidrug resistance was 13.0% (18/138) with the most common resistance profile being resistance to ampicillin-chloramphenicol-ciprofloxacin from Salmonella Enteritidis isolates (n=5). Resistance to first-line antibiotics limits the therapeutic choices for Salmonella infection. Relevant local antimicrobial resistance data on iNTS may support appropriate empiric therapy among vulnerable populations.

Human and animal brucellosis in the Sultanate of Oman: an epidemiological study

Brucellosis is an infectious disease caused by direct contact with infected animals or animal products contaminated with Brucella. Brucella is a Gram-negative aerobic coccobacillus that infects different types of animals and is considered to be an important zoonotic disease. Brucella were isolated from blood samples and identified following biochemical tests and agglutination with A and M monospecific antisera. Furthermore, Brucella antibody titers of the tested sera were obtained by the microtiter agglutination method (MAM). The main Brucella species isolated in Oman was B. melitensis. However, in countries bordering Oman and their neighboring countries, both B. melitensis and B. abortus have been isolated and identified. A total of 412 human patients with suspected cases of brucellosis were admitted to the Department of Communicable Disease Surveillance and Control in the Dhofar Governorate for diagnosis and treatment. During the year 2015, a total of 343 human cases were positively diagnosed with brucellosis in the Dhofar Governorate. During the years 2015 to 2019, a total of 10,492 animals were examined in different Governorates of Oman for brucellosis. The results indicated that 1161 (11%) animals were serologically positive for brucellosis. The results of this study confirmed that Brucella melitensis is the main species responsible for human brucellosis in Oman. It was not surprising that the Dhofar Governorate exhibited a high percentage of infected patients since it is culturally acceptable to drink raw camel milk (unpasteurized), unlike cow milk which is pasteurized.

Correlation between Coagulation Profile and Blood group among patients with Ischemic Heart Disease in Khartoum State

Background: Coronary artery disease (CAD), also known as ischemic heart disease (IHD) is a group of diseases that includes stable angina, unstable angina, myocardial infarction, and sudden cardiac death. It’s considered a major public health issue that affects an estimated 1 in 3 adults in the United States. The ABO blood group and coagulation profile exerts a profound influence on hemostasis, This study was aimed to correlate between ABO grouping & coagulation profile in Ischemic heart disease at Khartoum State. Methods: This was case and control study conducted during the period from January to May 2021 at Ahmed gasim hospital. Study included hundred (100) sample, fifty (50) Ischemic heart disease (IHD) patients taken as cases and fifty (50) apparently healthy taken as controls. Blood samples were collected for the analysis of prothrombin time (PT), activated partial thromboplastin time (aPTT) using STart stago semi-automated instruments and for the determining the ABO blood group using direct slide agglutination method. Statistical analysis was performed using SPSS version 25. Results: The results of study showed that the most frequent ABO blood type among all the participants among ischemic and healthy individuals was O. The mean level of APTT in Ischemic Heart Disease (IHD) showed that there was significantly decreased (27.06 ± 3.04) when compared to control group (29.45 ± 3.7) with p. value 0.001. However no significance differences in the mean level of PT between two groups (p value= 0.96). Its also observed that among all study participants, in blood group A, the PT and aPTT levels were the least, while blood group O had the greatest PT and aPTT values. No association was found between ABO grouping and coagulation profile in ischemic heart disease in case and control group. Conclusion: This study concluded that O blood group was more frequent among patients with IHD followed by A blood group and least frequent was AB. In addition, there was significant shorter aPTT in Ischemic Heart Disease cases when compared to control group. No association was found between ABO grouping and coagulation profile in ischemic heart disease in case and control group.

Frequency of ABO and Rhesus blood groups among blood donors of the Twin cities

More than 20 distinct blood group systems have been characterized; but the ABO and Rh blood groups remain the most important. The frequencies exhibit considerable variation in different geographic locations, reflecting the underlying genetic and ethnic diversity of the human population. The primary objective to carry out this study was to explore the frequency of ABO and Rh blood groups among the donors in the region of Islamabad and Rawalpindi, Pakistan. This cross-sectional study was carried out in the twin cities of Rawalpindi and Islamabad including data from 6th April 2019 to 15th September 2019. Data were collected retrospectively from the records of donors of all the registered blood banks in the mentioned localities during the last 5 years. Informed consent was gained and a total of 46363 participants were included with 25030 males and 21333 females. All participants were selected without any bias via the online patient record of the blood banks. All blood groups were identified using the slide agglutination method with commercially prepared anti-sera. The frequency of each blood group in order of its prevalence was found to be: B +ive (31.8%) > O +ive (28.5%) > A +ive (22.1%) > AB +ive (9%) > B -ive (3%) > O -ive (2.5%) > A -ive (2.1%) > AB -ive (0.9%). Overall blood group B +ive was the most common blood group, and Rh +ive groups were generally more common than Rh -ive blood groups. The results of our study provide useful data regarding frequencies of ABO and Rhesus blood groups among blood donors of the twin cities. It will in the future help create an inventory of blood banks, study disease patterns, and heredity in this region.

CHARACTERIZATION OF FOUR MAIN SALMONELLA SEROVARS, ALBANY, KENTUCKY, MBANDAKA, AND MONTEVIDEO, ASSOCIATED WITH CHICKEN LINES, FARMS, AND SAMPLE SOURCES IN SOUTHERN TAIWAN

Poultry and its products are often referred to as reservoirs of Salmonella to infect humans. Salmonella serovars were determined from samples of terminated eggs (early-terminated eggs, EEs, and late-terminated eggs, LEs) on farms YA (layers), YB (native hybrid broilers, NHCs), and YF (NHCs); one-day-old chicks (OCs) on farm YB; and shoe covers (SCs) on farms YB, YC (broilers), YD (broilers), YE (NHCs), and YF using multiplex PCR and agglutination methods. One hundred isolates were examined for susceptibility to 13 antimicrobials and profiles of pulsotypes and plasmids. Nine serovars were identified as serogroup B Agona and Schwarzengrund, serogroup C1 Montevideo and Mbandaka, serogroup C2 Albany, Kentucky and Newport, serogroup E Weltevreden, and serogroup G Kedougou. Different from those in 2003, Albany, Kentucky, Montevideo, and Mbandaka were the predominant serovars that differed in chromosomal and plasmid variations and antibiogram profiles. Albany and Kentucky isolates had higher resistance levels to the antimicrobials than the Montevideo and Mbandaka isolates. The antibiogram differences among the serovars may be related to the presence of antibiotic resistance genes in Salmonella genomic island 1-F (SGI1-F) and plasmids in Albany isolates. Albany and Kentucky exhibited the largest chromosomal and plasmid variations, respectively. The main serovars associated with embryogenesis termination were Mbandaka and Montevideo in EEs and LEs, Newport in NHCs, and Kedougou in LEs from layers. Furthermore, Montevideo and Agona were the only serovars detected in OCs, and Albany and Kentucky were the main serovars on SCs. Farm management also affected the Salmonella prevalence and predominant serovars.

Magnetic nanoparticles coated with zwitterionic copolymer as an advanced material for rapid and instrument-free biomolecular detection in human serum

The traditional agglutination assays especially those based on polystyrene beads have been recognized as convenient tools for disease diagnosis despite their limited detection range and low sensitivity. Unlike other particles namely polystyrene beads, SiO2 and gold nanoparticles having insignificant magnetic properties, magnetic nanoparticles (MNPs) offer unique advantages as their magnetic properties for agglutination methods. In the presence of magnet, not only can they be used to enrich the samples, but also their aggregation can also be induced, providing sensitive and rapid measurements. This work aims to develop MNPs for aggregation-based biomolecular detection. The MNPs were surface-modified with PMAMPC via an in situ coating method, then biotin as the target-specific probe was immobilized. The biotin-conjugated PMAMPC-MNPs were used for capturing and detecting the complementary protein, streptavidin in human serum samples. With the magnetic-induction, the nanoparticles would aggregate in the presence of streptavidin, resulting in a short detection time even in undiluted human serum. The concentration range for the detection was 35 nM to 150 nM and the lowest concentration of detection was 35 nM or equivalent to 2.5 mg⸳mL-1. The fact that this is simple, rapid and instrument-free method for biomolecular detection broadens their potential use in a variety of diagnostic applications.

Study of Fingerprint Pattern in Relation to Gender and Blood Grouping amongst the Medical Students of GMC Srinagar

A study was conducted on MBBS students in GMC Srinagar. A total of 100 students was included in the study ofboth genders. A proper informed consent was taken from participants. For collection of fingerprint, a tabulatedproforma was be used. Black printers ink was used for creating an imprint by rolling the finger on ink and thenon paper (rolling method) A cello tape was used to seal the print and a magnifying lens was used for aiding inobservation. Imprints from all ten fingers were collected. Information about blood groups was collected fromstudent identity card. In a few cases where the information was not known, blood grouping and Rh typing wasconfirmed by slide agglutination method using antiserum A, antiserum B and anti serum D. The aim of the studywas to find out the correlation between gender, fingerprint and blood grouping in students of GMC. Results: Wenoted that majority of the subjects belonged to blood group O (37.2%), followed by B+ve(24.5%) and A+ve(23.5.Maximum subjects (97%) were Rh positive. The rest were Rh negative. Females had highest percentage of loops(44%) followed by whorls (41%). Males had the highest percentage of whorls (55.5%) closely followed by loops(39.4%).Blood group O-ve is correlated to fingerprint whorl followed by AB+ve with whorls and B+ve with loops.A+ve is correlated to whorls and O+ve with whorls and B-ve with whorls. Blood group A showed highest whorls(45.4%) followed by loops (41.25%) and arches (10.42%). Also males had predominantly whorls (44.2%) followedby loops (39.4%) and females had predominantly loops(44.29%) followed by whorls (41.7%). the purpose of thisstudy is to correlate the relationship between various patterns of fingerprints, gender and “ABO” blood groupsand “Rh” blood types in students of GMC Srinagar. Conclusion: blood group, fingerprint and gender can only beassessed independently to secure identity if an individual.

Prevalence of Methicillin Resistance Staphylococci (MRS) from Neonatal Septicemia and Comparative Evaluation of Newer Rapid Latex Agglutination Method with Conventional Methods for diagnosis of MRS

Background & Aims: Neonatal septicemia is a major cause of neonatal mortality in developing countries. Rapid treatment with antimicrobials is very crucial for essential outcome. Methicillin Resistance Staphylococcus (MRS) are important cause of nosocomial infection, which causes septicemia especially in neonates. They are frequently resistance to other antibiotics also. So, rapid, sensitive and specific procedures are required urgently to initiate timely treatment. This study was undertaken to perform comparative evaluation of conventional methods with rapid agglutination method in diagnosis of MRS and to estimate the prevalence of MRS among neonatal septicemia cases. Materials and Method: This study was done in one of the major tertiary care hospitals in Ahmedabad, Gujarat. All the blood samples collected from the patients with sign and symptoms of septicemia, from January 2012 to July 2012, were cultured and identified by standard microbiological method. MRS detection was done by conventional methods and latex agglutination method. Results: Staphylococcus coagulase negative constituted 61.96% and S.aureus 4.33% of total bacterial isolates. Methicillin resistance in S.aureusand in CONS, was 44.44% and 25.91% respectively. Prevalence of MRS among neonates was 32.72%. The sensitivity and specificity of oxacillin disk was 68% and 100% respectively for detection of MRS. Latex agglutination test method shows sensitivity and specificity of 100% for detection of MRS. Conclusion: Along with cefoxitin disc diffusion method, latex agglutination method is to be used for early diagnosis & treatment and forprevention of over use of vancomycinfor methicillin resistant staphylococci. Keywords:Septicemia, MRS, Blood culture, S.aureus, Cefoxitin, CoNS

Prevalence and Specificities of Immune Red Cell Antibodies in Adult Patients with Sickle Cell Anaemia and Blood Donors in Uyo, South-South Nigeria: A Case-Control Study

Background: Sickle cell anaemia (SCA) is a major public health issue in Sub-Saharan Africa, including Nigeria. Transfusion of red blood cells is an essential therapeutic modality in SCA. Repeated RBC transfusions can cause alloimmunization resulting in haemolytic transfusion reactions, transfusion refractoriness among other complications. Aims and Objectives: The study aimed to determine the prevalence and specificities of immune erythrocyte alloantibodies among adult patients with SCA compared with healthy HbAA blood donors in Uyo, South-South Nigeria. Materials and Methods: All participants were interviewed using a structured questionnaire to obtain information on bio- data, blood transfusion history and other relevant SCA history. Antibody screening and identification were carried out using tube agglutination method with commercially made panel of cells. Results: A total of 160 subjects were studied. They were made up of 80 SCA patients and 80 healthy HbAA blood donors. Prevalence of red cell alloimmunization among multi-transfused patients with SCA was observed to be 11.3%. Alloantibodies identified were mainly against Rh antigens contributing 66.7% (anti-E 22.2%, anti-C 22.2%, anti-D 11.1% and anti-e 11.1%). Antibodies directed against Kell and Lutheran blood group antigens together constituted 33.3%. No antibody was detected in the controls. Advancing age (30 years and above) and ABO blood group were statistically associated with alloimmunization (P values of 0.043 and 0.013, respectively). Conclusion: Repeated blood transfusion is associated with the development of alloantibodies. Immunohaematologic tests in transfusion care of SCA patients should be improved to include extended red cell phenotyping and routine alloantibody screening and identification.

FREQUENCY AND DISRTIBUTION OF ABO AND RHESUS BLOOD GROUP IN NORTHERN AREAS OF KASHMIR VALLEY ADJAECENT TO LINE OF CONTROL.

Background: Distribution of blood groups varies demographically. ABO and Rh blood group systems are major clinically signicant blood group systems despite that more than 400 red cell antigens have been identied. Blood groups are genetically determined. ABO and Rh blood groups systems besides being most important in blood transfusion, are also important in forensic pathology, disease susceptibility and population genetics. This study aims to determine frequency and distribution of ABO and Rh blood groups among the permanent residents of northern areas of Kashmir valley adjacent to line of control. Aim and Objective: The present study was designed to determine frequency and distribution of ABO and Rh blood groups among blood donors in northern areas of Kashmir valley adjacent to line of control and compare the results with data available from studies conducted with in India and other countries. Material and Methods: This study was a two and half years prospective study conducted in the department of Pathology Government Medical College Baramulla from January 2020 to June 2022 . Only permanent residents of northern areas of Kashmir valley were included in the study. Blood donors who were not permanent residents of northern areas were excluded from the study. Blood donors were selected after proper history and complete examination. Blood donors fullling all eligibility criteria's for blood donation were selected. Determination of ABO and Rh status was done by tile method of agglutination using commercially available anti-Seras. Results: Out of total 4609 voluntary blood donors 4104 (89.04%) were Rh positive and 505 (10.96%) were Rh Negative. 1338 (29.03%) were O positive,1333 (28.92%) were B positive, 1025 (22.23%) were Apositive, 408 (8.8%) were AB positive, 187 (4.05%) were O negative, 156 (3.38%) were B negative, 102 (2.21%) were Anegative and 60 (1.30%) were AB negative. Conclusion: In the Northern areas of Kashmir valley adjacent to line of control O Positive is the most prevalent blood group followed by B positive and AB negative is least prevalent followed by Anegative.

Association of C-Reactive Protein (CRP) with Bipolar Disorder & Gender

Background: Bipolar disorder (BD) is a mental disorder with mood swings that fluctuate between mania, hypomania, and depression. C-reactive protein (CRP) levels in bipolar patients have been studied across the globe, but the results are variable. A very limited research has been conducted in this area in Pakistan. Therefore, the goal of the current investigation was to evaluate CRP in bipolar illness. Moreover any link between CRP and gender was also investigated. Materials and methods: In this cross-sectional study, 112 subjects (82 bipolar patients and 30 controls) were included. The patients were randomly selected from Punjab Institute of Mental Health (PIMH) and Sheikh Zayed Hospital, Lahore. After the consent of the subjects, venous blood samples were collected. CRP levels were semi-quantitatively estimated by agglutination method. The data obtained was analyzed through SPSS. Results: The difference in the mean CRP level among bipolar and control groups was statistically significant (p = 0.001). Moreover, males had higher CRP levels than females but the difference was non-significant (p=0.134). Comparison of CRP levels among various stages of BD and control showed a significant difference between the groups (p=0.009). Increased CRP levels give an idea about the inflammation and severity of the disease. CRP levels could be of help in the diagnosis and prognosis of BD. Conclusions: The present study suggests a significant association of CRP with bipolar disorder. No significant relation of CRP and gender was depicted. Keywords: Bipolar Disorder, C-reactive protein, Gender

Wider perspectives: It's a changing world-The use of ABO-incompatible plasma for resuscitating massively bleeding patients.

Wider perspectives: It's a changing world-The use of ABO-incompatible plasma for resuscitating massively bleeding patients.

ASSOCIATION BETWEEN ABO BLOOD GROUP AND OBESITY IN MEDICAL STUDENTS

Background — Over the past years, there seems to have been good attention paid to the connection between some diseases and the ABO blood group type.To our knowledge,nostudy has been done todetermine the association between obesity and ABO blood group. Aims — To find the prevalence of obesity in medical students and to determine the association between obesity and ABO blood group. Materials and methods — A cross sectional study was carried out among 745 medical students. Height, weight, waist circumference, hip circumference was measured. BMI and waist —hip ratio was determined.Blood group was determined by slide agglutination method Results — The findings of the present study reported that the most prevalent blood group was B+ whereas the blood group AB was least prevalent. 25.5% of the total study subjects were overweight and 7.2%were obese. Overweight and obesity were more common among students with blood group B.It was also observed that WHR statistically associated with ABO Blood group system. Conclusion — Participants with blood group B+ had higher prevalence of overweight and obesity. ABO Blood group system showed statistically significant association with WHR.

Molecular biology analysis of ABO blood group variants caused by natural chimaerism.

The chimaerism phenomenon constitutes a significant mechanism underlying ABO phenotype discrepancies; however, its detection has technical challenges. In the current study, we explored different techniques to establish the chimaeric status of ABO blood types. Fifteen individuals with possible chimaeric ABO blood type, as suggested by standard tube or column agglutination method and RBC adsorption-elution test, were enrolled in the study. The red blood cells from 11 investigated subjects showed mix-field agglutination with anti-A or anti-B in blood typing; weak A or B antigens on the other four individuals' RBCs were detected by adsorption-elution tests. The genetic study was conducted with PCR-SSP genotype, DNA sequencing of the ABO gene, STR analysis and ddPCR. The genetic chimaeric status was confirmed in four (27%) individuals by SSP test alone. The ABO gene sequencing identified an additional ABO allele and enabled chimaerism detection in 10 (67%) subjects. The STR analyses established the chimaerism status in 13 (87%) individuals. In the two cases where neither of the tests mentioned above had positive findings, the ddPCR was adopted, and microchimaerism, with an extremely low degree of chimaerism (0.77% and 0.12%), was revealed. The ddPCR revealed the unequal haplotypes (29.5% B vs. 70.5% O) in one subject and distinguished this B/O-O/O chimaera from certain B subgroups (B/O genotype without any mutation) like B3 . The ABO blood type chimaerism can be genetically established by comprehensive molecular methods, including PCR-SSP/DNA sequencing, STR and ddPCR, which is particularly sensitive for the detection of microchimaerism.

Clinical role of M. pneumoniae typing antibody detected by chemiluminescent immunoassay in the diagnosis of Mycoplasma pneumoniae pneumonia in children

ObjectiveThe levels of serum M. pneumoniae typing antibodies in children with community-acquired pneumonia (CAP) were detected by chemiluminescent immunoassay (CLIA) to explore the clinical role of M. pneumoniae typing antibody (MP-IgM, MP-IgG) in M. pneumoniae pneumonia. MethodsA total of 387 Child patients with CAP diagnosed at the Pediatric outpatient department of Zengcheng Branch, Nanfang Hospital, Southern Medical University, were enrolled between January 2020 to December 2021 and divided into M. pneumoniae pneumonia (MPP) group (n = 210) and non-M. pneumoniae pneumonia (NMPP) group (n = 177). Firstly, Clinical data, full blood count (WBC, NEU%, LYM%, MONO%, EOS%, BASO%, RBC, HGB, PLT) and biochemical tests (AST, LDH, ɑ-HBDH, CK, CKMB, CRP, PCT, IL-6) as well as laboratory diagnostic tests (MP-IgM, MP-IgG) were compared between the two groups. Secondly, we assessed the correlation between the average level of M. pneumoniae typing antibody detected by CLIA and the titer of anti-M. pneumoniae antibody (MP-Ab) tested by passive agglutination (PA) method. Thirdly, receiver operating characteristic (ROC) curve for the MP-IgM and MP-IgG was examined to evaluate the value of diagnosing M. pneumoniae pneumonia. Finally, we follow-up 120 cases of MPP group and analysis medication results. Results(1) Mean age, runny nose, expectoration, LYM%, NEU%, HGB, AST, MP-IgM and MP-IgG were statistically significant in the MPP group and NMPP group (all P < 0.05). (2) Correlation analysis showed that MP-IgM average level was linearly associated with MP-Ab titer (R2 = 0.84) and MP-IgG average level was exponentially correlated with MP-Ab titer under 1:640 (R2 = 0.96). (3) The ROC curve of MP-IgM and MP-IgG were significantly different (both P < 0.001). A serum MP-IgM level above 1 S/CO and MP-IgG level above 14.15 AU/mL were significant predictors for M. pneumoniae pneumonia: area under the curve (AUC) of 0.810, 0.815; standard error (SE) of 0.021, 0.022; 95 % confidence interval (CI) of 0.768–0.852, 0.773–0.858; the diagnostic sensitivity of 74.3 %, 62.1 %; and specificity of 72.9 %, 87.0 %; respectively. (4) Of the 120 children with M. pneumoniae pneumonia followed up, 79 (65.8 %) cases took azithromycin and 68 (86.1 %) cases were recovered. ConclusionsA series of our studies shown that, CLIA, speedy and automated clinical examination method, has higher specificity and sensitivity for the quantitative detection of MP-IgM and MP-IgG, playing an important role of early diagnosis as well as prompt intervention to reduces macrolide-resistant strains and sequelae of children with M. pneumoniae pneumonia.

Rheumatoid Arthritis and some Demographical and Clinical Parameters

Rheumatoid Arthritis is a heterogeneous, symmetrical chronic autoimmune inflammation of body joints, it infects the population with a ratio reaching 1% of the population's world, and it can lead to bone erosion and deformities, and finally damage. Smoking is one of the important factors that increase the risk of RA infections by aiding in citrullinated protein production, CRP increases in RA patients and binds with the severity of the disease. The aim of the study is to evaluate the CRP and smoking effects on RA patients. 150 blood samples were taken; 100 for RA patients and 50 for healthy control, the patients were taken from the Baghdad Teaching Hospital / Rheumatologically Consulting Clinic in the period from November 2021 to February 2022. The ages of patients range from 22 to 72 years old, and 26 to 62 years old was the age range of the healthy controls. 2ml of venous blood was taken in a gel tube for the CRP test by agglutination method. The result of the current study shows a highly significant differences between the RA patients and control groups in family history and CRP. While, it shows a non-significant difference for smoking. The conclusion of the current study was that CRP and family history associate with rheumatoid arthritis, and the smoking is not associate with RA disease.

Gambaran Pemeriksaan CRP pada Perokok Berat Pekerja Konveksi “X”

A convection worker is any person who works by receiving wages or other forms of remuneration in a place specially made to produce merchandise or jeans and apparel. Heavy smokers are people who smoke and immediately smoke cigarettes or people who smoke more than 20 cigarettes per day. Elevated levels of CRP associated with tobacco use Examination CRP is an acute phase protein that is formed in the liver due to an inflammatory process or infection. This study aims to determine the description of CRP in heavy smokers of convection workers "X" in Podo Village, Kedungwuni District, Pekalongan Regency. The type of research used is descriptive. The total population was 20, with inclusion and exclusion criteria, 17 samples were obtained for CRP examination using the agglutination method. The results showed that from 17 samples obtained negative CRP results. The results of CRP on heavy smokers of convection workers "X" in Podo Village, Kedungwuni District, Pekalongan Regency obtained negative results with a percentage of 100%.

Increasing seroprevalence but waning herd immunity against measles after elimination: Longitudinal seroepidemiology of measles in Osaka Prefecture, Japan, 2003–2020

Japan is one of the countries conducting longitudinal serosurveillance of vaccine-preventable diseases. We conducted surveillance of the local measles-specific antibody titer, calculated the effective reproduction number (Re), and compared data of four terms: term 1, 2003–2006 (before the introduction of the second shot of measles-containing vaccine); term 2, 2007–2010 (early term toward measles elimination); term 3, 2011–2014 (later term toward measles elimination); and term 4, 2015–2020 (after elimination of measles in Japan). Approximately 250 sera from volunteers aged 0 to ≥ 40 years were collected and examined for measles-specific IgG using the gelatin particle agglutination (PA) method annually from 2003 to 2020. Seroprevalence and the geometric mean of the PA antibody titer were examined by term. Re was calculated using the age-dependent proportion immune and contact matrix for each term. Of the 4,716 sera, 886 in term 1, 1,217 in term 2, 1,069 in term 3, and 1,544 in term 4 were collected. The seroprevalence gradually increased from term 1 (88.3% CI 86.0–90.3) to term 4 (95.7% CI 94.6–96.7), and the seroprevalence of term 1 was significantly lower than those of other terms (Fisher’s exact test, p < 0.001), with PA titer ≥ 16 as positive. By contrast, PA antibody titers significantly decreased from term 1 (median 1,024) to term 4 (median 256) (Mann–Whitney U test, p < 0.001). With the protection level (PA titer ≥ 128 and ≥ 256) as positive, Re gradually increased from term 1 (1.8 and 2.3) to term 4 (2.5 and 4.8, respectively). Waning levels of measles antibodies potentially increase the measles susceptibility in Osaka, Japan. This trend might imply a limitation of vaccine-induced immunity in the absence of a natural booster for wild strains after measles elimination. This study provides a cue for maintaining continuous measles elimination status in the future.

Difference of Clotting Time and Bleeding Time in the ABO Blood Groups

Introduction: Blood group, clotting time (CT) and bleeding time (BT) are vital haematological parameters that are tested prior to any surgery. Blood groups are associated with various diseases and blood group "O" linked with nose bleeds. The aim of this study is to evaluate the association between CT and BT between various blood groups and to find differences in gender among these parameters. Study Design: A cross-sectional observational study. Place and Duration: In the department of Physiology and Medicine, MTI LRH, Peshawar Pakistan for one-year duration from March 2021 to February 2022. Methods: This study was held amongst the 200 healthy individuals. The agglutination method is used for blood grouping, capillary tube method for CT and Dukes method for BT. Mean clotting and bleeding times (in seconds) were compared. Results: The study was conducted among 200 individuals (120 men, 80 women) aged 20-25 years. Although the changes were not statistically significant, the values of CT and BT were higher in blood group O, less BT in type B and AB blood grouping had lowest CT (p&gt; 0.17). Women had greater CT and BT values than men (p values &lt;0.001). There was also a significant association between the clotting time and bleeding time (Pearson's correlation coefficient, p = 0.512, p &lt;0.001). Conclusion: The conducted analysis showed a propensity to a greater jeopardy of haemorrhagic conditions and a lesser risk of thrombotic events in ‘’O’’ blood group in comparison to groups AB, A or B. A multicentre, bigger population study with an importance on determining plasma vWF levels may be useful for confirming results, identifying risk groups and taking preventive measures early. Keywords: Bleeding time (BT), ABO blood group, von Willebrand factor (vWF) and Clotting time (CT).