Age Classes Research Articles

Leaf Age Affects Mercury Accumulation in Evergreen Plants

We investigated the mercury (Hg) concentration of the full range of needle age classes (NACs) in two conifers, nine NACs in Picea abies and fourteen in Abies pinsapo var. marocana, as well as three leaf age classes (LACs) in two broadleaved evergreen species, Trochodendron aralioides and Rhododendron catawbiense. Additionally, the Hg concentration of the wooden branch segments to which the NACs were attached in the two conifers was studied. Picea abies showed a continued Hg accumulation over all NACs, but with an age-dependent decline in the accumulation rate. In Abies pinsapo var. marocana, maximum needle concentrations of Hg were reached after eight years. The concentration remained constant for NACs 9–14, indicating that needles had become saturated with Hg. The Hg concentrations of the branch segments were much lower than those of the needles in the older NACs. Over the three LACs of Trochodendron aralioides and Rhododendron catawbiense there was a steady increase in concentration with a weak indication of a declining Hg uptake rate in older leaves. The average needle/leaf lifetime Hg uptake rate per year was only half that of broadleaved species across all NACs and LACs. We conclude that in conifers maintaining a larger number of NACs there is a decline of the Hg accumulation rate in older NACs. In future biogeochemical research (empirical and modelling) and biomonitoring studies, the age of sampled leaves needs to be considered to account for the age dependence of leaf Hg concentration and accumulation rate.

Examination of Isotopic Signals to Determine Trophic Dynamics and Diet of Gulf of Maine Mysticetes Prior to an Oceanographic Regime Shift

Stable isotope analysis (SIA) is a useful tool to assess the health and foraging habits of large marine predators, metabolic stress, pregnancy, and migration patterns. This study provides baseline SIA data for four Gulf of Maine mysticete species and serves as a benchmark for future assessments. SIA was conducted on skin biopsies collected in two time periods: 1988 to 1992 (n = 15) and 1999 to 2005 (n = 187). Samples were collected from humpback whales (Megaptera novaeangliae; n = 116), fin whales (Balaenoptera physalus; n = 74), minke whales (Balaenoptera acutorostrata; n = 6), and North Atlantic right whales (Eubalaena glacialis; n = 6). There were statistically significant differences in isotopic value among species, years, and regions sampled. By species, North Atlantic right whale δ13C and δ15N levels were significantly different than the other species analyzed. Additionally, humpback whales had a δ15N value that was significantly higher than the value found in fin whales. By date, humpback whales showed significant difference in δ13C in 2002 from the two previous years. For fin whales, 2002 showed significant difference in δ13C for all other years’ samples (2000 to 2003). By region, two regions that were the greatest distance apart (Bay of Fundy and Great South Channel) showed significant differences in δ13C for humpback whales. Demographic analyses for humpback and fin whales found a significant difference between calves versus other age classes, presumably due to nursing. A decadal comparison of humpback whales at one site (Stellwagen Bank) found no significant difference between decades. This dataset provides a benchmark for stable isotope measurements in large baleen whales for this regional ecosystem.

The Tasmanian Estuary Perch Population—A Threatened Species?

Estuary perch (Percalates colonorum) are a long-lived and slow-growing species which are distributed throughout southeastern Australian estuaries. In Tasmania, only one known population remains which is located in the Arthur River, on the remote northwest coast. Tasmania is the only Australian state to list estuary perch as a wholly protected species (recreational and commercial), while other populations across their mainland distribution appear to be robust. A survey undertaken on estuary perch in the Arthur River in 2014–2015 indicated that the population was small (825–2375 fish) and dominated by three age classes between 12 and 14 years old. This population also has extremely low genetic diversity and is genetically distinct to mainland populations due to its geographic isolation. During a re-survey in December 2023, 378 estuary perch were captured, with 191 tagged and released, and 31 fish retained for age and gonad analysis. Although three strong age classes were detected (five, eight and twenty-three years), numerous weak and missing cohorts were apparent. This study also confirms this population has a delayed spawning season in comparison to mainland populations, with growth models and tag recaptures (32 fish) demonstrating a very slow but sexually dimorphic growth rate. By combining the datasets of the 2014/15 and 2023 survey, we present an updated understanding of the biology and population dynamics of this unique Tasmanian population, which has previously never been published. The results confirm the population is still reproductively active and stable; however, due to their restricted distribution, variable recruitment, slow growth, and small population, further conservation efforts are still required. Further legislative listings at both the state and federal levels are planned, which if successful will provide additional protection and assist in applications for external funding and resources. This will support the development of a recovery plan which will consider re-stocking efforts and re-establishment of previously inhabited waters and investigate potential actions to improve the genetics of the Tasmanian population. Additionally, educational campaigns to inform the public and recreational fishers on the status of estuary perch in Tasmania will be undertaken.

Sleep Preferences and Chronotype Traits Impact on Academic Performance Among University Students

Due to irregular daytime routines, chronotype changes, side jobs and exam periods after the COVID-19 pandemic, university students are trying to find new balances in their everyday life. The aim of the present study is to analyze the impact of daytime chronotype and hour preferences for the circadian rhythm on academic achievement among university students, considering their sleep habits and class frequencies in daytime and nighttime classes. Furthermore, this study aimed to analyze the differences in chronotype preferences with regard to age, sex, and academic schedule students are attending. A sample of 87 university second-year Psychology and Management students attending the academic year 2021/2022 after the governmental relief measures of the COVID-19 pandemic completed a 13-item questionnaire on sleep habits and preferences. Further variables encompassed sleep behaviors, such as age, sex, daytime and nighttime classes, as well as academic achievement. The results of the study showed that university students had a higher preference for the morningness type. Additionally, chronotype traits explained 30% (values from regression analysis) of academic achievement but did not directly impact academic results. The sleep-wake cycle diverged among age groups because the youngest participants (19–21 years old) and older participants (35–44 years old) reported higher academic scores during the first semester and the full academic year. No significant differences were identified with respect to sex. There is a lack of literature explaining the effect of sleep hours on academic achievement among students after stay-at-home rules during the COVID-19 pandemic. Thus, it is imperative to understand the difficulties students face with regard to their studying hours, working shifts, and daytime or nighttime classes to create a sustainable university system that attends to students’ needs and necessities.

Climate signal age effects in Pinus uncinata tree-ring density data from the Spanish Pyrenees

Key messageThe temperature sensitivity of maximum latewood density measurements in pine trees from a high-elevation site in the Spanish Pyrenees increases with tree age. Detrending modulates the intensity of the effect. Tree-rings are the prime archive for high-resolution climate information over the past two millennia. However, the accuracy of annually resolved reconstructions from tree-rings can be constrained by what is known as climate signal age effects (CSAE), encompassing changes in the sensitivity of tree growth to climate over their lifespans. Here, we evaluate CSAE in Pinus uncinata from an upper tree line site in the Spanish central Pyrenees, Lake Gerber, which became a key location for reconstructing western Mediterranean summer temperatures at annual resolution. We use tree-ring width (TRW) and maximum latewood density (MXD) measurements from 50 pine trees with individual ages ranging from 7 to 406 years. For MXD, temperature sensitivity increases significantly (p < 0.01) with tree age from r = 0.31 in juvenile rings with a cambial age < 100 years to r = 0.49 in adult rings > 100 years. Similar CSAE are not detected in TRW, likely affected by the overall lower temperature signal (rTRW = 0.45 vs. rMXD = 0.81 from 1951 to 2020). The severity of CSAE is influenced by the approach used to remove ontogenetic trends, highlighting the need to assess and consider potential biases during tree-ring standardization. Our findings reveal CSAE to add uncertainty in MXD-based climate reconstructions in the Mediterranean. We recommend studying CSAE by sampling diverse age classes in dendroclimatic field campaigns.

Do the biological characteristics of trout (Salmo trutta) smolts influence their spring migration timing and maiden marine sojourn duration?

Anadromous salmonids migrate seaward to exploit feeding and growth opportunities in marine habitats, yet how smolt biological characteristics influence their marine migratory behavior remains poorly understood. This study used 9 years of trout (Salmo trutta) population monitoring data from 15,595 tagged age-0+ parr, 1033 smolts detected migrating downstream in spring, and 99 adults detected returning from their first marine migration to the River Frome (Dorset, UK) to investigate the influence of smolt biological characteristics on their migration timing and maiden marine sojourn duration. Age-specific differences in the influence of smolt length on migration timing were found, with longer 1-year-old smolts emigrating later than their shorter counterparts within the same age class, but the opposite association existed for 2-year-old smolts. A bespoke integrated statistical model quantified the effects of smolt emigration day of year, age, sex, and length on the probability of first-time migrants returning to the river after one or more sea winters. Younger, later migrating smolts had a longer marine sojourn duration than their older, earlier migrating counterparts, and females remained at sea for longer periods than males. Although the statistical model was designed to maximize the use of information available in the data, it revealed only weak effects of smolt biological characteristics on the maiden marine sojourn duration. A complementary simulation study suggested that detecting more spring migrating smolts and analyzing longer time series of trout population monitoring data would increase the ability to detect statistically significant effects. Therefore, a strategic review of the trout population monitoring program, including more long-term biological data collection, is recommended. The modelling work presented here can provide guidance on the size of the required dataset and how to maximize the power of imperfect data.

Chlamydial infections in free-ranging raptors presenting to a university veterinary medical teaching hospital (1993-2022).

To describe the prevalence, clinical findings, lesions, and risk factors associated with chlamydial infections in free-ranging raptors presented to a university veterinary medical teaching hospital. Medical records retrospectively searched for raptors admitted from January 1993 through April 2022 were tested for Chlamydia spp infections using quantitative PCR (qPCR), immunohistochemistry, culture, and sequencing. Findings were collected and analyzed. Multivariable logistic regression analyzed the association between Chlamydia spp infection status and risk factors, including age class, species, sex, and season of admission. The prevalence for cases that tested positive for Chlamydia spp on 1 or more diagnostic tests, including mucosal qPCR samples for Chlamydia spp, tissue PCR for C buteonis, and mucosal qPCR genotyped as C buteonis, was 1.9% (74 of 3,983). All positive cases were from the genus Buteo (n = 74). Juvenile birds and winter season had higher odds of infection. All birds were in poor body condition (n = 74), often with moderate-to-severe CBC and biochemistry abnormalities consistent with multiorgan chronic inflammatory disease, emaciation, and dehydration. On postmortem examination of Chlamydia-positive birds (58 of 74), hepatitis (44 of 56), nephritis (24 of 39), splenitis (22 of 53), airsacculitis (21 of 43), myocarditis (21 of 39), and pneumonia (21 of 38) were common lesions, with intracellular bacteria in multiple tissues. Signalment, season of admission, clinical signs, clinicopathologic findings, and Chlamydia-specific testing identified chlamydial infections in free-ranging raptors. Appropriate protections to prevent potential zoonotic transmission in clinical wildlife rehabilitation settings are recommended. Many clinical parameters used to identify C psittaci infection in parrots can also be used to identify chlamydial infections in raptors.

Aposematism vs. scavenging? camera trapping unveils low and seasonal-dependent consumption of toxic salamander carcasses by wild boars

Abstract Aposematic species rely on primary and secondary antipredator strategies. Yet, some opportunistic and generalist species may consume them, although this has been largely supported by indirect evidence such as dietary analyses or clay model experiments. Here, we studied the role of the wild boar, Sus scrofa, as a consumer and possible predator of an aposematic and toxic salamander, Salamandra salamandra gallaica. We investigated this behaviour using camera traps and salamander carcasses on a small island where both species co-occur. Our results show a very low proportion of salamanders consumed by wild boars (3.8%), which indicates that fire salamanders might not completely rely on their aposematic strategy to avoid being consumed by this generalist species. The number of predation events does not differ between wild boar age classes, but it does between seasons, with a higher consumption in spring, which can be associated with an increase in animal consumption when fruits (e.g., acorns) are no longer available on this island. We also highlight the need for temporal studies targeting predator-prey interactions through behavioural data and complementary approaches to better understand the possible impact of opportunistic and generalist species in the ecosystem. This is particularly important in management plans involving the control of generalist species such as the wild boar, which is considered one of the most invasive species globally and a threat to countless species worldwide.

The Use of Humor, Irony, and Metaphors in Everyday Communication: An Exploratory Investigation

This study explores the role of humor, irony, and metaphors in everyday communication among college students at Mindoro State University (MinSU). These rhetorical strategies are integral to social interactions, helping individuals convey emotions, form connections, and simplify complex ideas. Using an exploratory, qualitative approach, the researchers examined how selected students employ these techniques across various social contexts, including family, friends, and academic settings. Data collection involved in-depth interviews, allowing for analysis of sociolinguistic factors, such as age, gender, and social class, that influence humor, irony, and metaphors. The findings suggest that humor fosters camaraderie, while irony subtly critiques or expresses contradictions, and metaphors enhance comprehension by linking abstract ideas to familiar experiences. Cross-cultural insights reveal unique interpretations of these elements, manifesting the potential for misunderstandings in diverse settings. This research highlights the adaptive nature of these rhetorical tools, revealing how they bridge social gaps, reinforce group identity, and support communication. The study contributes to understanding language use in regional academic settings, offering implications for effective communication and cultural sensitivity in multicultural environments.

Have People Treated With Antihypertensives Been Diagnosed With Hypertension? A Cross-Sectional Study in Stockholm, Sweden.

Studies on antihypertensive treatment are important, as hypertension remains the major risk factor for cardiovascular morbidity and premature death. However, antihypertensive medicines are also used for other conditions, and the use of these medicines as a proxy for a diagnosis of hypertension might lead to misclassification in pharmacoepidemiological studies. This study aimed to investigate to what extent people dispensed antihypertensive medicines have been diagnosed with hypertension. Cross-sectional study with data covering all healthcare and all dispensed prescriptions of antihypertensive medicines 2019 and diagnoses recorded 2015-2019 from the Stockholm Region, Sweden. Multinomial logistic regressions were used to assess the probability of having hypertension concerning age, sex, and antihypertensive drug class. A total of 386 860 individuals were included, 49% men, 12% incident users, and 80% of all had a recorded diagnosis of hypertension. In 73% of incident users, only one antihypertensive drug class was dispensed, as compared to 36% of prevalent users. A total of 38% of incident users and 9% of prevalent users had none of the diagnoses selected for the study recorded in any health record during 5 years. Prevalent and older users over the age of 65 from high (50%-79%) to very high (80% and more) probability of a recorded diagnosis of hypertension. Patients on antiadrenergic agents, high-ceiling diuretics, aldosterone antagonists, or beta receptor blockers had a lower probability of having a recorded diagnosis of hypertension than patients dispensed angiotensin-converting enzyme inhibitors, angiotensin receptor blockers, or calcium channel blockers. Most patients dispensed antihypertensive medicines have a diagnosis of hypertension. However, caution is needed using data on dispensed medicines to classify incident antihypertensive users and younger patients as having a diagnosis of hypertension.

STUDENTS HELPING OLLI BUILD A MORE DIVERSE MEMBERSHIP WITH AN ASSESSMENT OF AVAILABLE TRANSPORTATION OPTIONS

Abstract An undergraduate adulthood and aging class partnered with Osher Lifelong Learning Institute’s (OLLI) Diversity Committee to identify transportation resources available for seniors in their community to potentially increase the diversity of the OLLI membership. A needs assessment research framework was used to put a semester long, four-phase research project into place, each with specific goals and milestones. General milestones included researching available transportation resources and organizations for seniors; documenting target audiences and associated difficulties using said resources; exploring potential solutions and providing recommendations for future pilot projects. The Director of the OLLI program attended monthly report outs to assist in the creation of a doable pilot project, helping identify key constraints and logistics. The semester ended with a formal research report out, executive summary, and the university career counselor attending to help students brainstorm and create solid resume entries. The presentation will finish by describing what was learned via best practices and practices to avoid, particularly intergenerational “gaps” that surfaced on how to collect research, the types of research, and the breadth and depth of the research. Lastly lessons learned as a teacher on how to be in the “middle” of both generations and the OLLI director to help make the project a success and how to get student “buy in” by continually reviewing the importance of participating in a service-learning class project for future career aspirations.

Interspecific competition and survival pressures in endangered Barringtoniaracemosa populations of Mainland China

This study evaluates the growth, survival pressures, and community dynamics of Barringtonia racemosa (L.) Spreng. populations in Jiulong Mountain and Suixi County, Guangdong Province. Six distinct plant communities were identified, with human disturbances significantly disrupting natural succession processes. The population in Jiulong Mountain, particularly within the Talipariti tiliaceum-B. racemosa community (JLS-T), experienced higher survival pressures compared to Suixi County. Interspecific competition varied, with species like Derris trifoliata, T. tiliaceum, and invasives such as Ipomoea cairica and Mikania micrantha exerting substantial pressure on B. racemosa. Analysis of 234 B. racemosa individuals revealed significant correlations between diameter at breast height (DBH), plant height, and age structure distribution, with a linear relationship between DBH and height underscoring their relevance in understanding wood volume, biomass, and stand structure. Survival pressures were inversely related to DBH, indicating reduced competition as trees matured. Growth patterns exhibited an age-dependent plateau in height, potentially influenced by environmental and anthropogenic factors. Management strategies should prioritize the growth of individuals with DBH less than 5 cm (age classes I ~ II). These findings underscore the need for targeted conservation efforts to protect B. racemosa communities and sustain wetland ecosystems.

Climatic and edaphic influences on productivity and carbon sequestration of farm grown teak (Tectona grandis, linn.f) in Tamil Nadu, India

Teak (Tectona grandis, Linn.f) is one of the most sought-after tree crops due to the exceptional quality and high market value of its timber. Currently, the Indian subcontinent imports a significant quantity of teak to meet its growing demand. The growth and wood quality of teak are influenced by edaphic and climatic variations across sites, a factor that has been largely underexplored in research. The primary objective of the study is to analyse teak growth, wood quality, and carbon sequestration potential under farmland condition. To address this, the study was conducted across three agroclimatic zones of Tamil Nadu: North Eastern Zone (NEZ), North Western Zone (NWZ), and Cauvery Delta Zone (CDZ), focusing specifically on farmland conditions. The analysis encompassed biometric attributes, volume, and carbon sequestration potential of farm-grown teak across different age classes. The findings of the study reveal that among the three agroclimatic zones, the NEZ (15-20 years age class) recorded the highest biometric attributes, including mid diameter (MD) (0.193 m), volume (0.379 m3), heartwood proportion (0.239 m3), and carbon sequestration (0.683 Mg/acre).. Based on the overall performance, trees in the 15-20 years age class exhibited superior heartwood formation under farmland conditions. Therefore, this age class is recommended for teak cultivation in farmland conditions across Tamil Nadu to maximize growth, wood quality, and carbon sequestration potential.

Describing and Modelling Stem Form of Tropical Tree Species with Form Factor: A Comprehensive Review

The concept of tree or stem form has been central to forest research for over a century, playing a vital role in accurately assessing tree growth, volume, and biomass. The form factor is an essential component for expressing the shape of a tree, enabling more accurate volume estimation, which is vital for sustainable forest management and planning. Despite its simplicity, flexibility, and advantages in volume estimation, the form factor has received less attention compared to other measures like taper equations and form quotient. This review summarizes the concept, theories, and measures of stem form, and describes the factors influencing its variation. It focuses on the form factor, exploring its types, parameterization, and models in the context of various tropical species and geographic conditions. The review also discusses the use of the form factor in volume estimation and the issues with using default or generic values. The reviewed studies show that tree stem form and form factor variations are influenced by multiple site, tree, and stand characteristics, including site quality, soil type, climate conditions, tree species, age, crown metrics, genetic factors, stand density, and silviculture. The breast height form factor is the most adopted among the three common types of form factors due to its comparative benefits. Of the five most tested form factor functions for predicting tree form factors, Pollanschütz’s function is generally considered the best. However, its performance is often not significantly different from other models. This review identifies the “Hohenadl” method and mixed-effects modelling as overlooked yet potentially valuable approaches for form factor modelling. Using the form factor, especially by diameter or age classes, can enhance tree volume estimation, surpassing volume equations. However, relying on default or generic form factors can lead to volume and biomass estimation errors of up to 17–35%, underscoring the need to limit variation sources in form factor modelling and application. Further recommendations are provided for improving the statistical techniques involved in developing form factor functions.

Trace elements in feathers, pectoral plumage, and blood of griffon vultures (Gyps fulvus) from a captive environment of Southern Italy.

Heavy metals and metalloids are increasingly recognised as a threat to avian health, especially in species at the top of the food chain such as vultures. Griffon Vultures (Gyps fulvus) are ideal bioindicators for studying environmental contamination due to their scavenging habits and territorial behavior. In this study, we analysed the concentrations of six trace elements (Cd, Pb, Cr, Sb, Ni, and Cu) in feathers, pectoral plumage, and blood samples of Griffon Vultures from a captive environment in Sicily, Southern Italy, using an ICP-MS method. Significant differences in the levels of trace elements were observed between different matrices (p < 0.05). The ramigal feathers were divided into rachis (basal, medial, apical), calamus, and barbs (basal, medial apical). The Principal Component Analysis (PCA) demonstrated a clear differentiation in metal accumulation between the sections of feather matrices and between pectoral plumage and blood, reflecting the diverse pathways and time of exposure and deposition. No significant differences in heavy metal concentrations were observed between sexes and age classes. This work highlights the effectiveness of using feather matrices for monitoring environmental exposure to toxic elements and highlight the importance of continuous surveillance of heavy metal contamination in reintroduced vulture populations.

Negative interaction or coexistence? Livestock predation and conservation of wild carnivores in Kazinag National Park and adjacent region in the Kashmir Himalaya, India

Livestock predation by wild animals poses a significant challenge to communities residing in and around protected areas. This study aimed to assess the extent and patterns of livestock predation by Asiatic Black Bears and Leopards in villages around Kazinag National Park and adjoining areas: Limber Wildlife Sanctuary, Lachipora Wildlife Sanctuary, and Naganari Conservation Reserve, in Kashmir, India. Semi-structured questionnaire surveys and interviews conducted with residents and herders camping in the study area were used to collect data on livestock predation. A total of 72 livestock kills were documented for the years 2021 and 2022, involving Leopards and Black Bears. Statistical analysis revealed significant differences in predation patterns based on age class, livestock type, time &amp; place of events, injury pattern, and body part affected. Sheep were most frequently targeted, with total economic loss estimated at &gt;USD 15,000. Asiatic Black Bears primarily attacked at night and preferred cattle and sheep, while Leopards targeted goats and horses, peaking in summer and late autumn. The main factors influencing predation were grazing within the park and adjacent protected areas, and poorly constructed corrals. Mitigation strategies recommended include building robust corrals and designating specific grazing zones away from core wildlife habitats. The study emphasizes the need for comprehensive, context-specific approaches to ensure the long term human-wildlife coexistence in the region.

Population Growth and Age Structure of the Pikeperch &lt;i&gt;Sander lucioperca&lt;/i&gt; (Percidae) from the Lake Necheritsa (Sebezhsky National Park, Pskov Oblast)

The size, age, sex structures of pikeperch Sander (L.) from Lake Necheritsa (Sebezhsky National Park) have been studied, as well as linear growth using back-calculation according to the Leo direct dependence function. Growth of pikeperch is described formally by linear equation L ( t ) = 5.4 × x + 0.2 (cm). The length frequencies of individual age groups are normally distributed, which corresponds to ideas about linear fish growth. The population is represented by 13 age classes, the maximum length of an individual in the sample is 872 mm. The analysis of the diet showed the presence of 6 mass fish species observed in the catch, including the young pikeperch; the ratios of individual species in the diet and the sample indicates an absence of electivity of nutrition. According to the estimates, the proportion of pikeperch in the ichthyocenosis in the period 1989–2022 in the Lake Necheritsa has increased by ~ 8 times. A comparative analysis of the linear growth of pikeperch from populations of different regions under environmental conditions, differing in a number of values of key parameters, is carried out. Growth rates of pikeperch from southern populations are generally higher and more consistent with asymptotic growth, while the size limits and life expectancy are generally higher in northern populations. A hypothesis has been tested about the causes of differences in the nature of linear growth.

Anti‐Snake Behavior and Snake Discrimination in Vervet Monkeys

ABSTRACTAnti‐predator behavior can inform how animals classify potential threats and which cognitive mechanisms might be involved in risk assessment. Snakes are common predators for many primate species, yet most of our knowledge on primate anti‐snake behavior stems from predator model experiments. Only some studies could investigate natural predator–prey interactions. Here, we combine an observational study on anti‐snake behavior in free‐ranging vervet monkeys, Chlorocebus pygerythrus, with an experimental test of snake classification to achieve an integrated understanding of the monkeys' responses to and classification of snakes. Over 25 months, we gathered data from over 200 individuals in five groups and recorded natural encounters between vervet monkeys and five different species of snakes. We assessed whether the time that monkeys spent in close proximity (≤ 10 m) to snakes varied with the encountered species and whether age and sex classes differed in their propensity to approach snakes (≤ 10 m) or produce alarm calls. Encounters with pythons lasted longer, and monkeys were likelier to approach them than other species. While adult males were less likely to approach snakes, we found no effect of age or sex class on alarm‐calling probability. Alarm calling was restricted to individuals close to snakes, apparently facilitating the recruitment of nearby group members to a snake's position. In experiments, we tested whether vervet monkeys discriminated snake models by length or diameter, but found no effect of model size. While the experimental data showed the same trends as data from natural encounters, the model pythons were approached by a much larger proportion of subjects than real pythons. This contrast between responses in natural predator encounters and simulated events stresses how assessing the variation in natural encounters can provide valuable context when evaluating experimental data.

Role of protected areas for a colonial-breeding waterbird in a fragmented landscape throughout its annual cycle

ContextThroughout their annual cycle and life stages, animals depend on a variety of habitats to meet their vital needs. However, habitat loss, degradation, and fragmentation are making it increasingly difficult for mobile species such as birds to find suitable habitats. Wetlands are highly productive systems of great importance to many animals, but their continued degradation threatens their capacity to support different species, including waterbirds. In this context, waterbirds are likely to benefit not only from the creation and management of protected wetlands, but also from the existence of anthropogenic wetlands, managed for economic or recreational activities.ObjectivesWe investigated the habitat use of Eurasian spoonbills within an extensive and heterogeneous area in Southern France, and how it varies across the annual cycle and for different age classes.MethodsWe tracked 91 spoonbills of different ages throughout their annual cycle and tested for overall differences in the use of strongly protected areas in Camargue between periods and age classes. Additionally, we identified the main sites used and their management practices.ResultsOur study shows that privately managed wetland areas play a complementary role to strongly protected areas: they may provide spoonbills (and other waterbirds) with suitable foraging habitat at certain periods of the year when these are less available in strongly protected areas.ConclusionsThis study illustrates how the spoonbill, a moderately specialized species, is benefiting from current global changes due to its ability to use suitable habitats, natural and artificial, in fragmented landscapes. Nevertheless, reliance on privately managed wetland areas may have serious consequences for species that are highly dependent on them, and thus, habitat management promoting natural conditions may be crucial to maintain species resilience. It is therefore essential to understand how specific management actions may affect waterbird presence and habitat use, not only to enhance the effectiveness of conservation efforts, but also to promote wetland connectivity and species resilience, particularly in fragmented landscapes.

Cryospray reduces pain during venous cannulation in elective surgery patients: a randomized placebo-controlled study

BackgroundVenous cannulation is widely used in healthcare systems, and for many patients, it is painful and distressing. We hypothesized that the rapid onset of cryospray use would reduce pain from venous cannulation compared to the use of a placebo spray.MethodsThe trial was a prospective randomized placebo-controlled trial including 130 adult patients scheduled for elective surgery. Patients were randomized to receive either cryospray or placebo before venous cannulation. The primary outcome was patient-reported pain from vein puncture.ResultsThere were no differences in the baseline variables between the two groups with respect to age, sex, height, weight or ASA class. Patients in the cryospray group indicated more pain or discomfort with the application of the spray (0 (0-2.5)) than with the application of the placebo spray (0 (0–0)) (P < 0.005), as measured by the Numeric Rating Scale (NRS). Patients in the placebo group reported more pain with vein puncture than did those in the cryospray group (1 (0–3) vs. 3 [2, 3, 4–5], P < 0.005). When asked if the patient would have the same spray in case of canulation again, 57 patients from the cryospray group reported yes compared to 34 patients in the control group (P < 0.005).ConclusionsThis randomized study found that cryospray significantly reduced pain during venous cannulation without increasing procedure difficulty. Patients reported lower pain scores and a greater preference for cryospray in future procedures, supporting its use as an effective pain relief method in elective surgery.Trial registrationClinicalTrials. gov Identifier: NCT04865783 (28-04-2021).