Agent For Graphene Oxide Research Articles

Graphene oxide-coated metallic nanotube array as a surface-enhanced Raman scattering (SERS) substrate

This paper presents a highly-ordered metallic nanotube array (MeNTA) coated with graphene oxide (GO) to serve as a surface-enhanced Raman scattering (SERS) substrate. The MeNTA was fabricated using a top-down wafer-scale lithographic and sputter-deposition process, after which a thin layer of GO was deposited uniformly via dip coating. Scanning electron microscopy revealed that GO dip coating did not mar the highly ordered periodic structures on the MeNTA substrate. Raman scattering spectra of Rhodamine 6G (R6G) molecules revealed that coating MeNTA substrates with GO enhanced peak intensity by as much as 2.9 times. Among the substrates examined in this study, the Ag GO MeNTA presented the strongest SERS behavior, with a detection limit of 10−8 M and enhancement factors of 5.38 × 105 for R6G and 2.23 × 105 for Crystal Violet (CV). The uniform distribution of GO and array of periodic structures returned highly consistent (i.e., reproducible) analyte Raman signals, with a relative standard deviation of 12.7 % for R6G and 9.3 % for CV and excellent linear relationship between Raman signal intensity and analyte concentration (R2 = 0.9974 for R6G, 0.9844 for CV).

Eco-friendly fabrication of multifunctional magnetic plasmonic photocatalyst for adsorption, SERS monitoring and photodegradation of residual fluoroquinolone antibiotics in water

In this work, a kind of multifunctional magnetic plasmonic photocatalyst was prepared by a green and efficient process. Magnetic mesoporous anatase titanium dioxide (Fe3O4@mTiO2) was synthesized by microwave-assisted hydrothermal, and Ag NPs were simultaneously in-situ grown on Fe3O4@mTiO2 (Fe3O4@mTiO2@Ag), graphene oxide (GO) was then wrapped on Fe3O4@mTiO2@Ag (Fe3O4@mTiO2@Ag@GO) to increase its adsorption capacity for fluoroquinolone antibiotics (FQs). Owing to the localized surface plasmon resonance (LSPR) effect of Ag, as well as the photocatalytic capacity of TiO2, a multifunctional platform based on Fe3O4@mTiO2@Ag@GO was constructed for adsorption, surface-enhanced Raman spectroscopy (SERS) monitoring and photodegradation of FQs in water. The quantitative SERS detection of norfloxacin (NOR), ciprofloxacin (CIP), and enrofloxacin (ENR) was demonstrated with LOD of 0.1 μg mL−1, and the qualitative analysis was confirmed by density functional theory (DFT) calculation. The photocatalytic degradation rate of NOR over Fe3O4@mTiO2@Ag@GO was about 4.6 and 1.4 times faster than that of Fe3O4@mTiO2 and Fe3O4@mTiO2@Ag, indicating the synergetic effects of Ag NPs and GO, the used Fe3O4@mTiO2@Ag@GO can be easily recovered and recycled for at least 5 times. Thus, the eco-friendly magnetic plasmonic photocatalyst provided a potential solution for the removal and monitoring of residual FQs in environmental water.

Transparent wafer-scale self-standing fluorinated graphene films

The fluorination of self-standing graphene oxide (GO) films under mild conditions at 70 °C for 1h using a mixture of elemental fluorine and nitrogen gases (0.35 F2/0.65 N2 v/v) results in transparent, highly fluorinated (F/C = 1 as seen by XPS) graphene oxide (FGO) films which maintain initial densely packed structure and mechanical strength. Most of the oxygen atoms in the GO film were replaced by fluorine after fluorination, and two types of carbon-fluorine bonds coexist, i.e. primarily weakened C⋯F bonds and also pure covalent C–F bonds as evidenced by FTIR, XPS, NMR, and cyclic voltammetry when FGO is used as electrode in Li/electrolyte (PC:EC:3DMC/LiTFSI 1 M)/FGO cell similar to a lithium battery. To further understand the fluorination mechanism and to distinguish the reaction steps, xenon difluoride XeF2 was used as fluorinating agent for GO and in situ EPR and FTIR analyses suggest hydroxyl groups as preferential reactive sites. These self-standing transparent FGO films exhibit unusual electrochemical properties in lithium battery and can be used as hydrophobic lubricant membranes because of the low and stable coefficient of friction evidenced in the present work.

Prismatic Silver Nanoparticles Decorated on Graphene Oxide Sheets for Superior Antibacterial Activity.

Spherical silver nanoparticles (Ag NPs) and silver nanoprisms (Ag NPrsms) were synthesized and decorated on graphene oxide (GO) nanosheets. The Ag contents were 29% and 23% in the GO–Ag NPs and GO–Ag NPrsms, respectively. The Ag NPrsms exhibited stronger (111) crystal signal than Ag NPs. The GO–Ag NPrsms exhibited higher Ag (I) content (75.6%) than GO-Ag NPs (69.9%). Increasing the nanomaterial concentration from 25 to 100 µg mL−1 improved the bactericidal efficiency, and the antibacterial potency was in the order: GO–Ag NPrsms > GO–Ag NPs > Ag NPrsms > Ag NPs > GO. Gram-positive Staphylococcus aureus (S. aureus) was more vulnerable than Gram-negative Escherichia coli (E. coli) upon exposure to these nanomaterials. The GO–Ag NPrsms demonstrated a complete (100%) bactericidal effect against S. aureus at a concentration of 100 µg mL−1. The GO–Ag composites outperformed those of Ag or GO due to the synergistic effect of bacteriostatic Ag particles and GO affinity toward bacteria. The levels of reactive oxygen species produced in the bacteria–nanomaterial mixtures were highly correlated to the antibacterial efficacy values. The GO–Ag NPrsms are promising as bactericidal agents to suppress biofilm formation and inhibit bacterial infection.

Photocatalytic degradation of Janus Green Blue dye in wastewater by green synthesised reduced graphene oxide-silver nanocomposite

ABSTRACT In this study, green synthesis of reduced graphene oxide-Ag (rGO-Ag) nanocomposite was carried out via bioreduction of Ag salt and graphene oxide (GO) using waste dry cell battery rod and Corchorus olitorius extract as GO precursor and reducing agent, respectively. Characterisation of rGO-Ag nanocomposite was achieved by UV/Vis spectroscopy, XRD, SEM, FTIR and BET analyses. Photocatalytic degradation of Janus Green blue (JGB) dye was conducted in batch mode. Effects of irradiation time, photocatalyst composition, temperature and recyclability were investigated. BET surface area of the nanocomposite was 532.914 m2/g with pore size of 2.136 nm. Optimal percentage photo-removal and equilibrium adsorption capacity of JGB dye by the nanocomposite achieved were 95.72% and 120.48 mg/g, respectively. Photocatalytic adsorption process followed more accurately Freundlich model implying multilayer adsorption onto rGO-Ag surface. Adsorption kinetics fitted perfectly into pseudo-second-order model while the enthalpy change is endothermic. The photocatalyst exhibited considerable stability, efficiency and was successfully applied for the removal of JGB in wastewater samples.

Multifunctional magnetic Fe3O4/GO/Ag composite microspheres for SERS detection and catalytic degradation of methylene blue and ciprofloxacin

It is greatly significant to remove organic dyes and antibiotics in water because they are extremely harmful for human health and environment. In this work, we successfully developed magnetic Fe3O4/GO/Ag composite microspheres, using Fe3O4 microspheres as cores to wrap graphene oxide (GO) interlayer and further in situ depositing silver nanoparticles onto GO layer. The microspheres were characterized by SEM, XRD, XPS and Raman spectroscopy. As compared to Fe3O4/Ag microspheres, Fe3O4/GO/Ag composite microspheres displayed higher activity for SERS detection, and also catalytic reaction due to the synergistic interaction of GO nanosheets and Ag nanoparticles. Subsequently, the Fe3O4/GO/Ag composite microspheres were employed to quantitatively detect aromatic molecules, methylene blue (MB) in aqueous solution, showing the limit of detection was low down to 10−9 M and the correlation coefficients (R2) of the linear relationship between SERS intensities and logarithmic concentrations was high up to 0.91. Moreover, the Fe3O4/GO/Ag composite microspheres as catalysts completely degraded methylene blue and ciprofloxacin in 12 min with the presence of NaBH4. Finally, Fe3O4/GO/Ag composite microspheres were utilized to effectively follow the degradation reaction process of MB by SERS. Taken together, Fe3O4/GO/Ag composite microspheres present SERS application potentials in terms of trace and quantitative detection of chemicals, and in-situ monitoring catalytic degradation of organic pollutants.

Plasma-assisted in-situ preparation of graphene-Ag nanofiltration membranes for efficient removal of heavy metal ions

Graphene-based membranes have been considered as promising separation membranes for water treatments due to their unique two-dimensional confined channels. However, subject to the preparation technology, the effective construction of graphene-based filtration membranes with suitable separation ability on heavy metal ions still face considerable challenges. Herein, we have successfully constructed a kind of graphene-based (reduced graphene oxide, rGO) nanofiltration membranes by adopting a plasma-assisted in-situ photocatalytic reduction method. Graphene oxide-Ag (GO-Ag) composite sheets are prepared firstly and then assembled into membranes by vacuum filtration. With the use of Ag nanoparticles as plasmonic photocatalyst, GO-Ag films can be in-situ reduced, leading to the formation of rGO-based composite membranes. Thanks to the mild in-situ reduction process, the filtration ability on heavy metal ions (Cr(VI), Cr3+, Cu2+ and Pb2+) caused by lamellar structure is well retained in the as-formed rGO-Ag membranes. Especially, when treating the typical toxic Cr(VI) solution, the retention capacity, water flux and stability of rGO-Ag membranes are all improved compared with that of the original GO-Ag ones. In addition, the effectively rejection of Cr(VI) from mixed solutions containing both Cr(VI) and Cr(III) also suggests the good applicability of such rGO-Ag membranes in a complex wastewater system.

Detection of Imatinib Based on Electrochemical Sensor Constructed Using Biosynthesized Graphene-Silver Nanocomposite.

The establishment of a monitoring technique for imatinib is necessary in clinical and environmental toxicology. Leaf extracts of Lycoris longituba were used as reducing agent for the one-step synthesis of reduced graphene oxide-Ag nanocomposites. This nanocomposite was characterized by TEM, FTIR, XRD, and other instruments. Then, the graphene/Ag nanocomposite was used as a modifier to be cemented on the surface of the glassy carbon electrode. This electrode exhibited excellent electrochemical sensing performance. Under the optimal conditions, the proposed electrode could detect imatinib at 10 nM−0.28 mM with a low limit of detection. This electrochemical sensor also has excellent anti-interference performance and reproducibility.

Effect of carbonaceous nanofillers and triblock copolymers on the toughness of epoxy resin

The toughening of epoxy resins is a relatively recent trend in composite technology. The use of rigid carbonaceous fillers or plasticizing additives is well established in the literature, but their combined use and their effect on thermal or mechanical properties of composites is hardly studied. The aim of this work is to investigate the combination of neat and silane-treated carbonaceous fillers (graphene oxide (GO) or graphite nanoplatelets (GNP)) with poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) (EPE) triblock copolymers. The viscosity and curing behavior of the resin, as well as the dynamic mechanical and mechanical properties of the composites, were evaluated. Addition of EPE and GO slightly increased viscosity of the epoxy resin, and EPE acted as coupling and exfoliating agent of GO and GNP. EPE/GO-modified epoxy showed the lowest storage modulus and cross-link density, displaying in turn a more pronounced effect on impact strength and fracture toughness without sacrificing tensile and thermal properties. Treatment with APTES did not cause a significant effect on the composite properties.

Photothermal-assisted antibacterial application of graphene oxide-Ag nanocomposites against clinically isolated multi-drug resistant Escherichia coli.

In the field of public health, treatment of multidrug-resistant (MDR) bacterial infection is a great challenge. Herein, we provide a solution to this problem with the use of graphene oxide-silver (GO-Ag) nanocomposites as antibacterial agent. Following established protocols, silver nanoparticles were grown on graphene oxide sheets. Then, a series of in vitro studies were conducted to validate the antibacterial efficiency of the GO-Ag nanocomposites against clinical MDR Escherichia coli (E. coli) strains. GO-Ag nanocomposites showed the highest antibacterial efficiency among tested antimicrobials (graphene oxide, silver nanoparticles, GO-Ag), and synergetic antibacterial effect was observed in GO-Ag nanocomposites treated group. Treatment with 14.0 µg ml−1 GO-Ag could greatly inhibit bacteria growth; remaining bacteria viabilities were 4.4% and 4.1% for MDR-1 and MDR-2 E. coli bacteria, respectively. In addition, with assistance of photothermal effect, effective sterilization could be achieved using GO-Ag nanocomposites as low as 7.0 µg ml−1. Fluorescence imaging and morphology characterization uncovered that bacteria integrity was disrupted after GO-Ag nanocomposites treatment. Cytotoxicity results of GO-Ag using human-derived cell lines (HEK 293T, Hep G2) suggested more than 80% viability remained at 7.0 µg ml−1. All the results proved that GO-Ag nanocomposites are efficient antibacterial agent against multidrug-resistant E. coli.

Size-Dependent Antibacterial Activity of Silver Nanoparticle-Loaded Graphene Oxide Nanosheets.

A series of graphene oxide (GO) suspensions with different particle sizes (<100 nm, ~100 nm, ~1 µm and >1 µm) were successfully fabricated after 0, 30, 60 and 120 min of sonication, respectively. The antibacterial properties of GO suspensions showed that >1 µm GO size resulted in a loss of nearly 50% of bacterial viability, which was higher than treatment by ~100 nm GO size (25%) towards Escherichia coli (E. coli). Complete entrapment of bacteria by the larger GO was observed in transmission electron microscopy (TEM). Silver nanoparticles (Ag NPs) were doped onto GO samples with different lateral sizes to form GO–Ag NP composites. Resulting larger GO–Ag NPs showed higher antibacterial activity than smaller GO–Ag NPs. As observed by Fourier transform infrared spectroscopy (FTIR), the interaction between E. coli and GO occurred mainly at the outer membrane, where membrane amino acids interact with hydroxyl and epoxy groups. The reactive oxygen species (ROS) and the considerable penetration of released Ag+ into the inner bacterial cell membrane result in loss of membrane integrity and damaged morphology. The present work improves the combined action of GO size effect with constant Ag loadings for potential antibacterial activity.

A sustainable reduction route of graphene oxide by industrial waste lignin for versatile applications in energy and environment

The high toxicity of chemical reducing agents for graphene oxide (GO) and the high-polluting emission of industrial waste lignin as black liquor are always the two troubling issues in clean production. Herein, we reported a sustainable win-win route to utilize waste lignin as a green reducing agent of GO through hydrothermal method. Various hydrothermal conditions were investigated to reveal the reduction capability and mechanism of lignin towards GO. Results indicated that lignin could repair the conjugated structure of GO, while the hydroxyl ions in water could cause the decarboxylation of GO. The lignin-reduced GO composites (LRGOs) prepared at acidic and neutral pHs tended to form hydrogels, while the alkaline LRGOs were monodispersed graphene nanosheets in water. The alkaline LRGO suspensions exhibited reversible sol-gel transition by adding acid/base, and irreversible gelation by metal ions chelation. Besides, they could self-assemble into a robust film with excellent mechanical properties (306.7 ± 47.9 MPa in tensile strength, 135.2 ± 45.3 GPa in modulus) and electrical conductivity (399.5 ± 58.3 S/cm) through vacuum filtration and annealing. The freeze-dried LRGO aerogels and their carbonized products showed excellent absorption capacities for various organic solvents. As electrode materials, the LRGO aerogels also displayed a high pseudo-capacitance of 213.8 F/g at 1 A/g. These findings of LRGOs showed a huge application potential in clean energy and environmental pollution treatment.

Nitrogen-Doped Graphene: The Influence of Doping Level on the Charge-Transfer Resistance and Apparent Heterogeneous Electron Transfer Rate.

Three nitrogen-doped graphene samples were synthesized by the hydrothermal method using urea as doping/reducing agent for graphene oxide (GO), previously dispersed in water. The mixture was poured into an autoclave and placed in the oven at 160 °C for 3, 8 and 12 h. The samples were correspondingly denoted NGr-1, NGr-2 and NGr-3. The effect of the reaction time on the morphology, structure and electrochemical properties of the resulting materials was thoroughly investigated using scanning electron microscopy (SEM) Raman spectroscopy, X-ray powder diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS), elemental analysis, Cyclic Voltammetry (CV) and electrochemical impedance spectroscopy (EIS). For NGr-1 and NGr-2, the nitrogen concentration obtained from elemental analysis was around 6.36 wt%. In the case of NGr-3, a slightly higher concentration of 6.85 wt% was obtained. The electrochemical studies performed with NGr modified electrodes proved that the charge-transfer resistance (Rct) and the apparent heterogeneous electron transfer rate constant (Kapp) depend not only on the nitrogen doping level but also on the type of nitrogen atoms found at the surface (pyrrolic-N, pyridinic-N or graphitic-N). In our case, the NGr-1 sample which has the lowest doping level and the highest concentration of pyrrolic-N among all nitrogen-doped samples exhibits the best electrochemical parameters: a very small Rct (38.3 Ω), a large Kapp (13.9 × 10−2 cm/s) and the best electrochemical response towards 8-hydroxy-2′-deoxyguanosine detection (8-OHdG).

Fabrication of Reduced Graphene Oxide-Ag Nanocomposites and Analysis on the Interaction with BSA

Graphene is an excellent platform to support and stabilize silver nanoparticles (AgNPs). The reduced graphene oxide-silver nanoparticles (rGO-AgNPs) were synthesized by the chemical reduction method and characterized by using ultraviolet-visible (UV-vis) absorption, transmission electron microscopy (TEM), X-ray diffractometer (XRD), and scanning probe microscopy (SPM). The binding reaction of rGO-AgNPs with bovine serum albumin (BSA) was investigated by using fluorescence spectrometry and SPM. As the concentration of AgNPs increased, the fluorescence spectrum was quenched, and the quenching process of rGO-AgNPs and BSA was static quenching. Thermodynamic parameters of the absorption process were evaluated at different temperatures, and the negative values of Gibbs free energy ( Δ G ) showed that this process was spontaneous. The main type of interaction was hydrophobic interaction according to the values of changes in standard enthalpy ( Δ H ) and entropy ( Δ S ). In addition, the morphology changes of proteins interacting with nanomaterials were detected by SPM.

A highly sensitive competitive immunosensor based on branched polyethyleneimine functionalized reduced graphene oxide and gold nanoparticles modified electrode for detection of melamine

Branched polyethyleneimine functionalized reduced graphene oxide (BPEIGn) was prepared by a one-step reaction, catalyzed by NaOH, using branched polyethyleneimine (BPEI) and graphene oxide (GO) without reductant hydrazine hydrate or sodium borohydride. The branched polyethylenimine acted as both a grafting agent and a reducing agent of GO. An competitive electrochemical immunosensor based on the Au/sodium mercaptopropanesulfonate/BPEIGn/gold nanoparticles/melamine (Au/MPS/BPEIGn/AuNPs/Mel) modified electrode was constructed for the determination of melamine. The double amplification of BPEIGn and AuNPs increased the sensitivity of the sensor. The melamine was detected by differential pulse voltammetry (DPV) in buffer solution (pH 7.4) containing K3(Fe(CN)6]/K4[Fe(CN)6]. Under optimized conditions, the proposed melamine immunosensor showed a linear relationship in the concentration range of 1 × 10−6 to 1 μM, with a detection limit of 2.66 × 10−7 μM.

DFT Study of N2O Adsorption onto the Surface of M-Decorated Graphene Oxide (M = Mg, Cu or Ag).

In order to reduce the harm of nitrous oxide (N2O) on the environment, it is very important to find an effective way to capture and decompose this nitrous oxide. Based on the density functional theory (DFT), the adsorption mechanism of N2O on the surfaces of M-decorated (M = Mg, Cu or Ag) graphene oxide (GO) was studied in this paper. The results show that the effects of N2O adsorbed onto the surfaces of Mg–GO by O-end and Cu–GO by N-end are favorable among all of the adsorption types studied, whose adsorption energies are −1.40 eV and −1.47 eV, respectively. Both adsorption manners belong to chemisorption. For Ag–GO, however, both the adsorption strength and electron transfer with the N2O molecule are relatively weak, indicating it may not be promising for N2O removal. Moreover, when Gibbs free energy analyses were applied for the two adsorption types on Mg–GO by O-end and Cu–GO by N-end, it was found that the lowest temperatures required to undergo a chemisorption process are 209 °C and 338 °C, respectively. After being adsorbed onto the surface of Mg–GO by O-end, the N2O molecule will decompose into an N2 molecule and an active oxygen atom. Because of containing active oxygen atom, the structure O–Mg–GO has strong oxidizability, and can be reduced to Mg–GO. Therefore, Mg–GO can be used as a catalyst for N2O adsorption and decomposition. Cu–GO can be used as a candidate material for its strong adsorption to N2O.

Synthesis and catalytic performance of graphene oxide-Au nanorings composites

A simple and facile method is described for the in situ synthesis of graphene oxide-Ag nanoplates (GO-AgNPs) hybrid structure and then served as sacrificial templates for producing graphene oxide-Au nanorings (GO-AuNRs) by galvanic replacement taken place on the GO surface. The catalytic activity of the resulting hybrid nanomaterial has been investigated for the reduction of 4-nitrophenol (4-NP) to 4-aminophenol (4-AP) with sodium borohydride (NaBH4). The results showed that the rate constant of GO-AuNRs nanomaterials was 0.205[Formula: see text]min[Formula: see text] and the samples showed significant enhancement in catalytic activity.

Graphene oxide mediated reduction of silver ions to silver nanoparticles under environmentally relevant conditions: Kinetics and mechanisms

We systematically investigated the reduction mechanisms and reduction kinetics of silver ions (Ag ions) by graphene oxide (GO) under ambient condition. UV–vis spectroscopy, transmission electron microscopy, and electron diffraction results revealed that silver nanoparticles (Ag NPs) could be formed from aqueous Ag ions in the presence of GO at pH 8 under light. Formation of Ag NPs increased with increasing pH (7.4, 8, and 9) and temperature (from 30 to 90); however, the increasing ionic strength and dissolved oxygen reduced the Ag NPs yield. The Ag ions reduction by GO followed pseudo-first-order kinetics under both dark and light, and light irradiation significantly accelerated the Ag NPs formation induced by GO. The phenolic-OH on GO was the dominating electron donator for Ag ion reduction in dark. Exposure to light increased the concentration of phenolic-OH on the GO surface, thereby stimulating the reduction rate of Ag ions by GO. In addition, the light induced electron-hole pairs on GO surface and light activated oxygen-centered radicals on GO surface promoted the reduction of adsorbed Ag ions by GO. Our findings provide important information for the role of GO in reducing Ag ions to Ag NPs in aquatic environments, and shed light on understanding the environmental fate and risk of both Ag ions and GO materials.

Preparation and photocatalytic activity of Ag-modified GO-TiO2 mesocrystals under visible light irradiation

Nanosized metallic Ag particles modified graphene oxide-TiO2 mesocrystals (Ag/GO-TMCs) were successfully synthesized by photoreduction deposition method. The morphology, crystallization, structural features and chemical compositions of the as-prepared Ag/GO-TMCs catalysts were investigated using HRTEM, XRD, Raman spectra, XPS, UV–vis DRS, PLS, and N2 adsorption-desorption measurements. Ag nanoparticles were found to be evenly distributed on the surface of TiO2 mesocrystals (TMCs) and graphene oxide (GO). The Ag/GO-TMCs catalysts featured high crystallinity, abundant mesoporous structure, large surface area, and improved visible-light response. Photocatalytic applications of the catalysts for degradation of Rhodamine B (RhB) and dinitro butyl phenol (DNBP) under visible light irradiation were investigated. The results showed that the highly enhanced photocatalytic activity was obtained with Ag/GO-TMCs catalysts. The Ag/GO-TMCs photocatalyst with AgNO3/GO-TMCs mass ratio of 0.075:1 exhibited the highest photocatalytic degradation of RhB and DNBP under 3 h of visible light irradiation. The superior photocatalytic activity of Ag/GO-TMCs may be attributed to the higher visible-light absorbance ability and surface plasmon resonance (SPR) effect of Ag nanoparticles and GO. The Ag-GO/TMCs materials could be deemed as promising photocatalysts for organic contaminants removal under visible light irradiation, in assessing their high photocatalytic efficiency and good repeatability.

Functionalized titanium implant in regulating bacteria and cell response.

Biological complications are an issue of critical interest in contemporary dental and orthopedic fields. Although titanium (Ti), graphene oxide (GO) or silver (Ag) particles are suitable for biomedical implants due to their excellent cytocompatibility, bioactivity, and antibacterial properties, the exact antibacterial mechanism is not understood when the three substances are combined (Ti-GO-Ag). In this work, the material characterization, antibacterial property, antibacterial mechanisms, and cell behavior of Ti-GO-Ag fabricated by electroplating and ultraviolet reduction methods respectively, were investigated in detail. The material char acterization of Ti-GO-Ag tested by atomic force microscopy, Raman spectroscopy, X-ray photoelectron spectroscopy, nanoindentation, nanoscratch, inductively coupled plasma mass spectrometer, and contact angle tester revealed the importance of GO concentration and Ag content in the preparation process. The antibacterial tests of Ti-GO-Ag clearly demonstrated the whole process of bacteria interacting with materials, including reactive oxygen species, endocytosis, aggregation, perforation, and leakage. In addition, the behavior of Ti-GO-Ag showed that cell area, length, width, and fluorescence intensity were affected. Briefly, Ti-GO-Ag nanocomposite was a dual-functionalized implant biomaterial with antibacterial and biocom patible characterization.