African Lakes Research Articles

Phylogeography and Ecological Niche Shape the Cichlid Fish Gut Microbiota in Central American and African Lakes.

Cichlid fishes, with their repeated colonization of lakes and subsequent radiations at different scales of phylogenetic and ecological diversification, offer an excellent model system to understand the factors shaping the host-gut microbiota association in nature. Here, we characterized the gut microbiota of the Amphilophus species complex from Central America (known as the Midas cichlid complex), encompassing 158 wild specimens (13 species) collected from seven Nicaraguan lakes, and combined these data with previously published data from two African lakes (spanning 29 species). Our aim was to comprehensively explore trends in microbiota variation and persistence along the large spatial and temporal scales of cichlid diversification (from the oldest radiation in L. Tanganyika, 9–12 My old, to young ones in Nicaraguan crater lakes, <0.5 My old), in allopatry and sympatry (within and across lakes), and across the range of dietary niches (from highly specialized to generalist feeders). Despite their extraordinary diversity, cichlids shared a remarkably conserved microbial taxonomic profile, which argues for a primary role of the host genetics in the assembly and maintenance of these microbial communities. Within this partly constrained microbiota profile, geographic isolation (continent and lake) represented the first level of discrimination. For the Midas cichlid, a partial congruency was found between host microbiota and genetic distances, suggesting that microbial communities have partly diversified along their cichlid phylogeographic history of crater lake colonization. In sympatry (within lakes), the young and poorly ecologically diversified cichlid assemblages of Central American lakes display largely unresolved gut microbiotas (in terms of both alpha and beta diversities), whereas the phylogenetically and ecologically diverse species found in African lakes showed greater microbial interspecific diversity. This pattern largely points to the level of habitat segregation, trophic niche overlap, and reproductive barriers as major modulators of the gut microbiota connectivity among sympatric species.

Towards sustainable fisheries and aquaculture management in the African Great Lakes

AbstractInland fisheries play important roles in food and economic security in the riparian countries surrounding the Great Lakes of Africa. However, the lakes are being systematically degraded by anthropogenic pressures, in combination with the huge population growth prevalent in the region. This paper summarises the outcomes of an international conference to develop a ‘Strategy for Conservation and Sustainable Development of the African Great Lakes Region in a Changing Climate’, held in Uganda in 2017, with particular attention on the potential for fisheries and aquaculture. The paper highlights options for addressing the problems facing the aquatic resources and specifically the importance of effective management of the fisheries and ecosystems of the Great Lakes to achieving the UN Sustainable Development Goals to ensure food and nutritional security and sustainable livelihoods. There is need to improve fisheries assessment and determine the value of the fisheries (and aquaculture). Fisheries legislation, regulation and enforcement need revision and co‐management mechanisms require rethinking. There is considerable potential for aquaculture, and especially cage culture, to increase fish production, but there is an urgent need to address technical, social, environmental and input requirements. It is also imperative that best practices guidelines are developed that will support cage culture production practices.

Factors associated with neonatal mortality in the African Great Lakes region: A pooled analysis of national surveys

# Background Neonatal mortality is a major public health problem and accounts for about one third of the deaths of children under-5 years. The aim of this study was to estimate the rate and predisposing factors associated with neonatal mortality in the African Great Lakes region (Burundi, Congo Democratic Republic, Kenya, Rwanda, Tanzania and Uganda). # Methods The study used cross-sectional data from the most recent Demographic and Health Survey of countries in the African Great Lakes region. A total sample of 56600 neonatal deaths of singleton live-born infants was reported across the region. Neonatal mortality was defined as the death of a newborn within the first 28 days of life. Cox regression model was used to identify factors associated with neonatal mortality in the African Great Lakes region. # Results The Democratic Republic Congo had the highest neonatal mortality rate per 1000 births (19, 95% confidence interval (CI)=17-21) within the region followed by Uganda (18, 95% CI=16-21) and Tanzania (16, 95% CI=14-19). Multilevel logistic regression analysis shows that factors significantly associated with neonatal mortality were: Sex of child (male neonates; hazard ratio (HR)=0.79, 95% CI=0.65-0.96), perceived birth size (small neonates; HR=1.88, 95% CI=1.47-2.39), birth order and birth interval (first child; HR=3.05, 95% CI=2.28-4.07), 4th or more child with \> 2 years birth interval (HR=0.48, 95% CI=0.36-0.66), previous death of baby (HR=10.3, 95% CI=7.75-13.68), non-usage of contraceptives (HR=1.81, 95% CI=1.43-2.29), non-receipt of tetanus toxoid injection (HR=1.36, 95% CI=1.04-1.78), no antenatal care visits (HR=1.62, 95% CI=1.06-2.48), and delivery through caesarean section (HR=1.89, 95% CI=1.40-2.55). # Conclusions To meet the Sustainable Development Goals (SDGs) target for neonatal mortality, interventions should focus on increasing access to good quality healthcare services for women and their newborns as well as family planning services and home-based neonatal care services.

Factors associated with neonatal mortality in the African Great Lakes region: A pooled analysis of national surveys

# Background Neonatal mortality is a major public health problem and accounts for about one third of the deaths of children under-5 years. The aim of this study was to estimate the rate and predisposing factors associated with neonatal mortality in the African Great Lakes region (Burundi, Congo Democratic Republic, Kenya, Rwanda, Tanzania and Uganda). # Methods The study used cross-sectional data from the most recent Demographic and Health Survey of countries in the African Great Lakes region. A total sample of 56600 neonatal deaths of singleton live-born infants was reported across the region. Neonatal mortality was defined as the death of a newborn within the first 28 days of life. Cox regression model was used to identify factors associated with neonatal mortality in the African Great Lakes region. # Results The Democratic Republic Congo had the highest neonatal mortality rate per 1000 births (19, 95% confidence interval (CI)=17-21) within the region followed by Uganda (18, 95% CI=16-21) and Tanzania (16, 95% CI=14-19). Multilevel logistic regression analysis shows that factors significantly associated with neonatal mortality were: Sex of child (male neonates; hazard ratio (HR)=0.79, 95% CI=0.65-0.96), perceived birth size (small neonates; HR=1.88, 95% CI=1.47-2.39), birth order and birth interval (first child; HR=3.05, 95% CI=2.28-4.07), 4th or more child with \> 2 years birth interval (HR=0.48, 95% CI=0.36-0.66), previous death of baby (HR=10.3, 95% CI=7.75-13.68), non-usage of contraceptives (HR=1.81, 95% CI=1.43-2.29), non-receipt of tetanus toxoid injection (HR=1.36, 95% CI=1.04-1.78), no antenatal care visits (HR=1.62, 95% CI=1.06-2.48), and delivery through caesarean section (HR=1.89, 95% CI=1.40-2.55). # Conclusions To meet the Sustainable Development Goals (SDGs) target for neonatal mortality, interventions should focus on increasing access to good quality healthcare services for women and their newborns as well as family planning services and home-based neonatal care services.

Key Factors of Precipitation Stable Isotope Fractionation in Central-Eastern Africa and Central Mediterranean

The processes of isotope fractionation in the hydrological cycle naturally occur during vapor formation, vapor condensation, and moisture transportation. These processes are therefore dependent on local and regional surface and atmospheric physical features such as temperature, pressure, wind speed, and land morphology, and hence on the climate. Because of the strong influence of climate on the isotope fractionation, latitudinal and altitudinal effects on the δ18O and δ2H values of precipitation at a global scale are observed. In this study, we present and compare the processes governing precipitation isotope fractionation from two contrasting climatic regions: Virunga in Central-Eastern Africa and the Central Mediterranean (Stromboli and Sicily, Italy). While Virunga is a forested rainy tropical region located between Central and Eastern Africa, the Mediterranean region is characterized by a rainy mild winter and a dry hot summer. The reported δ18O and δ2H dataset are from precipitation collected on rain gauges sampled either on a monthly or an approximately bimonthly basis and published in previous papers. Both regions show clearly defined temporal and altitudinal variations of δ18O and δ2H, depending on precipitation amounts. The Central Mediterranean shows a clear contribution of local vapor forming at the sea–air interface, and Virunga shows a contribution from both local and regional vapor. The vapor of Virunga is from two competing sources: the first is the continental recycled moisture from soil/plant evaporation that dominates during the rainy season, and the second is from the East African Great Lakes evaporation that dominates during the dry season.

Divergence in larval jaw gene expression reflects differential trophic adaptation in haplochromine cichlids prior to foraging

BackgroundUnderstanding how variation in gene expression contributes to morphological diversity is a major goal in evolutionary biology. Cichlid fishes from the East African Great lakes exhibit striking diversity in trophic adaptations predicated on the functional modularity of their two sets of jaws (oral and pharyngeal). However, the transcriptional basis of this modularity is not so well understood, as no studies thus far have directly compared the expression of genes in the oral and pharyngeal jaws. Nor is it well understood how gene expression may have contributed to the parallel evolution of trophic morphologies across the replicate cichlid adaptive radiations in Lake Tanganyika, Malawi and Victoria.ResultsWe set out to investigate the role of gene expression divergence in cichlid fishes from these three lakes adapted to herbivorous and carnivorous trophic niches. We focused on the development stage prior to the onset of exogenous feeding that is critical for understanding patterns of gene expression after oral and pharyngeal jaw skeletogenesis, anticipating environmental cues. This framework permitted us for the first time to test for signatures of gene expression underlying jaw modularity in convergent eco-morphologies across three independent adaptive radiations. We validated a set of reference genes, with stable expression between the two jaw types and across species, which can be important for future studies of gene expression in cichlid jaws. Next we found evidence of modular and non-modular gene expression between the two jaws, across different trophic niches and lakes. For instance, prdm1a, a skeletogenic gene with modular anterior-posterior expression, displayed higher pharyngeal jaw expression and modular expression pattern only in carnivorous species. Furthermore, we found the expression of genes in cichlids jaws from the youngest Lake Victoria to exhibit low modularity compared to the older lakes.ConclusionOverall, our results provide cross-species transcriptional comparisons of modularly-regulated skeletogenic genes in the two jaw types, implicating expression differences which might contribute to the formation of divergent trophic morphologies at the stage of larval independence prior to foraging.

African lakes Nios and Mone — indicators of unique carbon element-deep respiration of the Earth

As a result of the analysis of space, geological and tectonic information, it was established that the true causes of natural ecolymnological disasters in Cameroon in 1984 and 1986 were modern fault-block tectonic movements, which are closely associated with seismic and geodynamics in one of the sections of the “living” Adamawa Mountains. The main cause of catastrophes must be considered the activation of endogenous processes occurring in the mantle and the tectonosphere of the Earth. Cosmo- and rotogenesis of the planet Earth, in the near-surface parts of the Earth’s crust of the Adamava mountain segment, led to intensive mountain-building and heat exchange processes, the causes and mechanism of which are closely associated with the rise of abnormally hot magmatic material and gas-liquid fluids containing CO2 from the mantle. Favorable transport routes for heat and mass transfer in the Earth’s lithosphere are volcanic channels, as well as the orthogonal and diagonal network of deep faults. At the same time, volcanic channels should be considered as unique drain pipes of our planet. The lethal carbon dioxide ejected from the depths of Lakes Nyos and Monun is mainly a differentiate of igneous melts, and the latter, in turn, have mantle “roots” extending to a depth of 200–300 km. The volcano-crater lakes Nios and Monun are confined to the nodes of the intersection of “living” deep faults, revealing the deep horizons of the planet, where in magmatic foci CO2 is predominant as products of differentiation. The author proposed a mechanism for the formation of a solid gas hydrate shell, a relatively tightly sealed volcanic crater. This giant gas hydrate plug prevented the gradual-passive circulation, i. e. outflow of CO2 into the hydrosphere and atmosphere coming from deep and intermediate magmatic foci. So, under the gas hydrate shell of the lakes Nios and Monun, a large amount of CO2 accumulated. Explosive emissions of significant amounts of lethal gas could appear only with the geodynamic activation of the earth’s crust, where these unique volcano-crater lakes are located. Seismotectonic processes contributed to the destruction of the gas hydrate shell and the breakthrough of CO2 through fractures, cracks and through the water membrane to the surface. Emissions of gases on the volcano-crater lakes Nyos and Monun are the brightest example (indicator) of the Earth’s carbon dioxide-deep degassing.

Political Myths of the African Great Lakes Region

Political Myths of the African Great Lakes Region

Assessing the vulnerability of Africa's freshwater fishes to climate change: A continent-wide trait-based analysis

Climate change is a key driver of biodiversity loss across the globe, and freshwater fishes are predicted to be among the most vulnerable taxa. African freshwater ecosystems are home to one of the most unique and diverse ichthyo-faunas on the planet, and freshwater fish species provide essential livelihoods for millions of people living in riparian communities across the continent. Although nearly one sixth of African freshwater fishes have been designated as endangered or vulnerable to extinction by the IUCN Red List assessment, the effects of climate change on these species have not been explored on a continent-wide scale. In this study, we present the first trait-based climate change vulnerability assessment (CCVA) comprising the majority (85%) of Africa's currently described freshwater fishes. We assembled data relating to three dimensions of vulnerability including sensitivity, adaptive capacity, and exposure. In addition, we developed an index of ‘conservation value’ based on traditional conservation metrics including extinction risk, endemism, and provision of ecosystem services. We found that almost 40% of African freshwater fishes are vulnerable to climate change, mostly owing to the many species with highly specialized habitat and life-history requirements, and because of the numerous anthropogenic stressors they face. High proportions of species within the Nothobranchiidae and Cichlidae families were found to be vulnerable. Regions with high frequencies of vulnerable species included the African rift valley lakes, the Congo River drainage, and the coastal rivers of West Africa. Several important data deficiencies were identified relating to species' population sizes, genetic variability, and life history traits, and constitute priority research areas for the future. In addition, we highlighted some cases where traditional conservation approaches overlook species and regions that are predicted to be threatened by climate change.

Bacterial GMGTs in East African lake sediments: Their potential as palaeotemperature indicators

Glycerol monoalkyl glycerol tetraethers (GMGTs) are a group of membrane spanning lipids produced by some species of archaea and bacteria. They differ from the more commonly studied glycerol dialkyl glycerol tetraethers (GDGTs) in having an additional covalent carbon-carbon bond connecting the two alkyl chain. The relative abundance and distribution of bacterial branched GMGTs (brGMGTs) in surface sediments from a set of East African lakes were studied. The abundance of brGMGTs relative to the brGDGTs is positively correlated to measured mean annual air temperature (MAAT), although with a significant amount of scatter. BrGMGT abundance was not correlated to lake water pH. Seven major brGMGTs that vary in degree of methylation were identified, with m/z 1020, 1034 and 1048. Further, the mass chromatograms of the m/z 1020 and 1034 brGMGTs show an interesting distribution of peaks, which likely relates to the occurrence of distinct brGMGT isomers. This structural complexity is higher than previously observed in peats and marine sediments. Principal component analysis of the fractional abundance of bacterial tetraether lipids revealed the brGMGTs behave similarly to one another but differently from both the 5- or 6-methyl brGDGTs. This suggests the brGMGTs are produced by a common source organism and are methylated at a different position. The distribution of the seven brGMGTs showed considerable correlation with MAAT. This variability was captured in a new proxy index (the brGMGTI), which showed a strong positive linear relationship with MAAT. Lacustrine brGMGTs show potential to be applied to ancient settings to provide information about paleoclimate. © 2019 The Authors

Typology of Environmental Impacts of Artisanal and Small-Scale Mining in African Great Lakes Region

Artisanal and small-scale mining is a widespread economic sector in the African Great Lakes Region, where it has an adverse impact on the population’s environment. The purpose of this paper is to summarize and consider the typology of the environmental impacts of artisanal and small-scale mining, in particular, the anthropogenic influences on topography with regard to the methods used in raw material mining. Among the most significant environmental aspects related to artisanal and small-scale mining are deforestation, changes in landscape structure, influence over geomorphological processes and hydrological river regime, chemical pollution of soil and watercourses, influencing soil production capacity. The aforementioned factors can cause health problems such as silicosis, poisoning by methyl orthophosphate, or injury during the mining activity itself. Artisanal and small-scale mining could initiate new geomorphological processes or modify naturally occurring geomorphological processes. These dynamic processes are influenced by the topography of the relief, soil properties, and rock composition. Anthropogenic activity in these cases may lead to faster reshaping (degradation or abrasion) of soil shapes. This study covers a broad understanding of environmental impacts of artisanal and small-scale mining with a focus on anthropogenic influencing.

The lobed-lipped species of Haplochromis (Teleostei, Cichlidae) from Lake Edward, two instead of one

Thickened lips have evolved several times within the cichlid flocks of the East African Great Lakes. This distinct and easily recognisable phenotype is a model trait to study convergent evolution. Lake Edward (Eastern Africa) contains a unique cichlid assemblage, which has remained largely understudied. Hitherto, only one cichlid species with lobed lips, Haplochromis labiatus, was known from this lake. This species has a blunt snout and rounded and mostly retrognathous jaws. However, we found specimens with lobed lips, but with acute snouts and slender and mostly prognathous jaws. These belong to a species, hitherto unknown to science: H. lobatus sp. nov. Both species occur sympatrically. We evaluated the morphological diversity within these species by investigating a total of 112 specimens using traditional and geometric morphometric approaches. Both species are formally (re)described and show a relatively large variation in their trophic morphologies, which complicates identification. Both have a small gape, stout outer teeth that strongly decrease in size laterad, and a lower jaw set anteriorly with procumbently-implanted outer teeth. Haplochromis labiatus differs from H. lobatus sp. nov. by a straight to convex vs. straight to concave head, a slightly shorter and broader lower jaw (27.7–34.3 vs. 31.2–40.7% HL; 71.4–92.4 vs. 48.5–70.5% LJL), and lobed lips that are thickened uniformly over their whole lengths vs. medially enlarged. Gut content observations revealed that both species have an insectivorous diet. While both exploit similar food sources, their morphological differences presumably prevent them from entering into direct ecological competition.

East African cichlid lineages (Teleostei: Cichlidae) might be older than their ancient host lakes: new divergence estimates for the east African cichlid radiation

BackgroundCichlids are a prime model system in evolutionary research and several of the most prominent examples of adaptive radiations are found in the East African Lakes Tanganyika, Malawi and Victoria, all part of the East African cichlid radiation (EAR). In the past, great effort has been invested in reconstructing the evolutionary and biogeographic history of cichlids (Teleostei: Cichlidae). In this study, we present new divergence age estimates for the major cichlid lineages with the main focus on the EAR based on a dataset encompassing representative taxa of almost all recognized cichlid tribes and ten mitochondrial protein genes. We have thoroughly re-evaluated both fossil and geological calibration points, and we included the recently described fossil †Tugenchromis pickfordi in the cichlid divergence age estimates.ResultsOur results estimate the origin of the EAR to Late Eocene/Early Oligocene (28.71 Ma; 95% HPD: 24.43–33.15 Ma). More importantly divergence ages of the most recent common ancestor (MRCA) of several Tanganyika cichlid tribes were estimated to be substantially older than the oldest estimated maximum age of the Lake Tanganyika: Trematocarini (16.13 Ma, 95% HPD: 11.89–20.46 Ma), Bathybatini (20.62 Ma, 95% HPD: 16.88–25.34 Ma), Lamprologini (15.27 Ma; 95% HPD: 12.23–18.49 Ma). The divergence age of the crown haplochromine H-lineage is estimated to 22.8 Ma (95% HPD: 14.40–26.32 Ma) and of the Lake Malawi radiation to 4.07 Ma (95% HDP: 2.93–5.26 Ma). In addition, we recovered a novel lineage within the Lamprologini tribe encompassing only Lamprologus of the lower and central Congo drainage with its divergence estimated to the Late Miocene or early Pliocene. Furthermore we recovered two novel mitochondrial haplotype lineages within the Haplochromini tribe: ‘Orthochromis’ indermauri and ‘Haplochormis’ vanheusdeni.ConclusionsDivergence time estimates of the MRCA of several Tanganyika cichlid tribes predate the age of the extant Lake Tanganyika basin, and hence are in line with the recently formulated “Melting-Pot Tanganyika” hypothesis. The radiation of the ‘Lower Congo Lamprologus clade’ might be linked with the Pliocene origin of the modern lower Congo rapids as has been shown for other Lower Congo cichlid assemblages. Finally, the age of origin of the Lake Malawi cichlid flock agrees well with the oldest age estimate for lacustrine conditions in Lake Malawi.

Molecular phylogeny of Oreochromis (Cichlidae: Oreochromini) reveals mito-nuclear discordance and multiple colonisation of adverse aquatic environments

Although the majority of cichlid diversity occurs in the African Great Lakes, these fish have also diversified across the African continent. Such continental radiations, occurring in both rivers and lakes have received far less attention than lacustrine radiations despite some members, such as the oreochromine cichlids (commonly referred to as ‘tilapia’), having significant scientific and socio-economic importance both within and beyond their native range. Unique among cichlids, several species of the genus Oreochromis exhibit adaptation to soda conditions (including tolerance to elevated temperatures and salinity), which are of interest from evolutionary biology research and aquaculture perspectives. Questions remain regarding the factors facilitating the diversification of this group, which to date have not been addressed within a phylogenetic framework. Here we present the first comprehensive (32/37 described species) multi-marker molecular phylogeny of Oreochromis and closely related Alcolapia, based on mitochondrial (1583 bp) and nuclear (3092 bp) sequence data. We show widespread discordance between nuclear DNA and mitochondrial DNA trees. This could be the result of incomplete lineage sorting and/or introgression in mitochondrial loci, although we did not find a strong signal for the latter. Based on our nuclear phylogeny we demonstrate that adaptation to adverse conditions (elevated salinity, temperature, or alkalinity) has occurred multiple times within Oreochromis, but that adaptation to extreme (soda) conditions (high salinity, temperature, and alkalinity) has likely arisen once in the lineage leading to O. amphimelas and Alcolapia. We also show Alcolapia is nested within Oreochromis, which is in agreement with previous studies, and here revise the taxonomy to synonymise the genus in Oreochromis, retaining the designation as subgenus Oreochromis (Alcolapia).

The risk posed by Xanthomonas wilt disease of banana: Mapping of disease hotspots, fronts and vulnerable landscapes.

Banana production landscapes in the African Great Lakes Region (AGLR) have been under immense pressure from Xanthomonas wilt (XW) disease over the past two decades. XW, first reported on banana in central Uganda and eastern DR Congo in 2001, has since spread to the entire AGLR. XW is currently spreading westwards from hot spots in eastern DR Congo highlands, putting the plantain (Musa AAB genome) belt of central and west Africa at risk. In-depth understanding of the key variables responsible for disease spread, current hotspots, and vulnerable landscapes is crucial for disease early warning and management. We mapped aggregated disease distribution and hotspots in the AGLR and identified vulnerable landscapes across African banana production zones. Available data on disease prevalence collected over 11 years was regressed against environmental and expert developed covariates to develop the AGLR XW hotspots map. For the Africa-wide risk map, precipitation, distance to hotspots, degree of trade in fresh banana products, production zone interconnectedness and banana genotype composition were used as covariates. In the AGLR, XW was mainly correlated to precipitation and disease/banana management. Altitude and temperature had unexpectedly low effects, possibly due to an overriding impact of tool-mediated spread which is part of the management covariate. In the AGLR, the eastern part of DR Congo was a large hotspot with highest vulnerability. Apart from endemic zones in the AGLR and Ethiopia, northern Mozambique was perceived as a moderate risk zone mainly due to the predominance of ‘Bluggoe’ (Musa ABB type) which is highly susceptible to insect-vectored transmission. Presence of XW hotspots (e.g. eastern DR Congo) and vulnerable areas with low (e.g. north-western Tanzania) or no disease (e.g. Congo basin, western DR Congo and northern Mozambique) pressure suggest key areas where proactive measures e.g. quarantines and information sharing on XW diagnosis, epidemiology, and control could be beneficial.

Recurrent changes in cichlid dentition linked to climate‐driven lake‐level fluctuations

AbstractClimate‐induced habitat change has often been invoked as an important driver of speciation and evolutionary radiation in cichlid fishes, yet studies linking morphological change directly to long‐term environmental fluctuations are scarce. Here, we track changes through time in the oral dentition of Oreochromis hunteri, the endemic and only indigenous fish species inhabiting the East African crater Lake Chala (Kenya/Tanzania), in relation to climate‐driven lake‐level fluctuations during the last 25,000 yr. Fossil teeth were recovered from six periods representing alternating high and low lake‐level stands associated with late‐Pleistocene and Holocene trends in rainfall and drought. To construct a reference framework for morphological variation in fossil assemblages, we first analyzed the variability in oral tooth morphology within the modern‐day fish population. These analyses established that, like other cichlids, O. hunteri gradually replace their bicuspid/tricuspid oral dentition with more unicuspid teeth as they grow. In the fossil assemblages, we found systematic and recurrent changes in the relative abundance of these oral tooth types, with a higher proportion of unicuspids during low‐stands. Moreover, O. hunteri living during low‐stands systematically developed unicuspid dentition at a smaller body size, compared with conspecifics living during high‐stands. Considering that low‐stand conditions created a sizable area of oxygenated soft‐bottom habitat that is lacking during high‐stands, we propose that the associated change in oral dentition reflects the fishes’ exploitation of this new habitat either for feeding or for reproduction. The recurrent nature of the observed shifts provides evidence for the ability of O. hunteri to systematically adapt to local habitat change, and strongly suggests that morphological change in oral dentition promoted its long‐term population persistence in an aquatic ecosystem presenting the dual challenge of being both sensitive to climate change (creating constantly shifting selective pressures) and isolated from surrounding populations (limiting gene flow). In Lake Chala, ancient climate‐driven lake‐level fluctuations did not directly promote speciation in O. hunteri, but the population bottlenecks they may have caused potentially contributed to its divergence from sister species Oreochromis jipe.

Phylogeography of Bellamya (Mollusca: Gastropoda: Viviparidae) snails on different continents: contrasting patterns of diversification in China and East Africa

BackgroundSpecies diversity is determined by both local environmental conditions that control differentiation and extinction and the outcome of large-scale processes that affect migration. The latter primarily comprises climatic change and dynamic landscape alteration. In the past few million years, both Southeast Asia and Eastern Africa experienced drastic climatic and geological oscillations: in Southeast Asia, especially in China, the Tibetan Plateau significantly rose up, and the flow of the Yangtze River was reversed. In East Africa, lakes and rivers experienced frequent range expansions and regressions due to the African mega-droughts. To test how such climatic and geological histories of both regions relate to their respective regional species and genetic diversity, a large scale comparative phylogeographic study is essential. Bellamya, a species rich freshwater snail genus that is widely distributed across China and East Africa, represents a suitable model system to address this question. We sequenced mitochondrial and nuclear DNA for members of the genus from China and used published sequences from Africa and some other locations in Asia to investigate their phylogeny and distribution of genetic diversity.ResultsOur phylogenetic analysis revealed two monophyletic groups, one in China and one in East Africa. Within the Chinese group, Bellamya species show little genetic differentiation. In contrast, we observe fairly deep divergence among the East African lakes with almost every lake possessing its unique clade. Our results show that strong divergence does not necessarily depend on intrinsic characteristics of a species, but rather is related to the landscape dynamics of a region.ConclusionOur phylogenetic results suggest that the Bellamya in China and East Africa are independent phylogenetic clades with different evolutionary trajectories. The different climate and geological histories likely contributed to the diverging evolutionary patterns. Repeated range expansions and regressions of lakes likely contributed to the great divergence of Bellamya in East Africa, while reversal of the river courses and intermingling of different lineages had an opposite effect on Bellamya diversification in China.

Understanding gender roles and practices in the household and on the farm: Implications for banana disease management innovation processes in Burundi

Banana and plantain are one of the most important staple food crops and a significant source of income to smallholder farmers in the East African Great Lakes Region. Banana Xanthomonas Wilt (BXW) is a devastating bacterial disease that threatens smallholder production and livelihoods. We use a systems approach to describe how gender shapes roles and practices in the household, on the farm and in innovation processes. We draw on a case study in Burundi, where single disease stem removal (SDSR) has been introduced as a labour-saving package to reduce BXW incidence. Banana is grown by an estimated 90% of farmers, and BXW poses a critical threat to food security. We use qualitative data that include focus group discussions, interviews and transcripts from farmer learning group (FLG) discussions to describe gender norms, roles and practices and implications for awareness and uptake of SDSR in households. We identify gender patterns in innovation process, namely that men gain higher levels of access to information in FLGs than women and men are also primarily responsible for implementing SDSR. These patterns reflect gender-differentiated norms, roles and practices that are common in the household and in banana-based farming systems, thus demonstrating the ways that innovation processes perpetuate and reinforce common gender roles and practices. Women’s participation in FLGs, albeit lower than men, increases the potential of women to implement specific practices of the SDSR package. Systems approaches may be similarly used in different contexts where awareness and uptake of banana disease management packages, and other technical innovations, are not well understood. We found that gender norms, roles and practices significantly influence uptake of SDSR practices and warrant further investigation across the region, where smallholder uptake remains a pressing challenge to establish household food security.

John Francis Talling. 23 March 1929—20 June 2017

John (Jack) Francis Talling was a master limnologist who pioneered much of our understanding of the River Nile, the great lakes of the African Rift Valley and those of the English Lake District. He was one of the world's leading authorities on the ecophysiology of freshwater phytoplankton and specialized in the control of their productivity by light and carbon dioxide. His perspectives were formed by interaction with leading scientists of the day, mainly at the Freshwater Biological Association, Cumbria, but also at laboratories in Africa and at the Scripps Institution of Oceanography, California. Jack's work on the effects of light on phytoplankton productivity was based on detailed measurements of the underwater light climate in lakes and oceans as well as laboratory and field measurements of the response of phytoplankton photosynthesis to light, involving the development of new or improved measurement methods. Calculation procedures were developed to estimate how light controls primary productivity. He devised the widely used characteristic ‘ I k ’ to quantify the onset of light saturation in the curve that defines the response of photosynthesis to light quantity. Experiences in extremely productive African soda lakes stimulated an interest in the possibility of CO 2 being a limiting factor controlling phytoplankton productivity and, more generally, Jack had the ecological insight to recognize that ecological dynamics resulted from interactions among factors rather than a response to a single variable.

Evidence for the existence and diversification of a new group of cichlids in the Miocene lakes of the Tugen Hills, Kenya

Evidence for the existence and diversification of a new group of cichlids in the Miocene lakes of the Tugen Hills, Kenya