Affect Soil Health Research Articles

Healthcare waste management and antimicrobial resistance: a critical review

ABSTRACT The rapid growth of populations and urbanization has led to a significant increase in healthcare waste, posing serious health risks. A search on Google Scholar identified seven relevant articles from Ethiopia that examine the relationship between improper waste management in healthcare facilities (HCFs) and the rise of antimicrobial resistance (AMR) genes. This review aims to highlight key concepts, evidence sources, and knowledge gaps specific to the Ethiopian context. The unsafe disposal of antibiotics through leaks and solid waste has contributed to what some are calling a ‘silent pandemic,’ raising concerns about emerging infectious diseases. Studies have revealed alarming rates of infectious agents and AMR in healthcare wastewater. Isolates of C. jejuni, Escherichia coli, Enterococcus faecalis, and Enterococcus faecium from various healthcare waste sites in Ethiopia demonstrate high levels of AMR genes. Additionally, research indicates that HCFs produce significant amounts of waste, with high per-person daily waste production rates. Leachate from landfills containing this waste can negatively affect soil health, biological activity, water quality, agriculture, animal health, and human well-being. To mitigate these risks, effective waste management practices and the promotion of alternative antimicrobial use are essential strategies for reducing the emergence of pandemic diseases in developing countries.

Circular economy in action: Transforming textile waste into sustainable soil additives - Physicochemical properties and biodegradability

In recent years, there has been an increasing interest in soil additives used in environmental ecosystems. Currently, most soil amendments are produced from synthetic materials. Some synthetic amendments break down into microplastics, which persist in the environment and can be ingested by soil organisms, affecting soil health and biodiversity and the chemicals they contain can leach into groundwater. At the same time, the number and availability of biodegradable technologies are limited, and the aspects related to their biodegradation in soil are not sufficiently known. The study developed a sustainable water-saving and vegetation-supporting technology in the form of a biodegradable water-absorbing geocomposite (BioWAG). BioWAGs were produced using biodegradable materials derived from animal and plant waste, such as waste wool, jute, and linen. In a three-year field experiment, their physicochemical properties in the soil were analysed. These were characterized using a.o. Surface weight, scanning electron microscopy (SEM), and FTIR. The results indicate that the degree of biodegradation of materials used in BioWAG depended on the raw material and production technology. Needle-punched nonwovens underwent intensive biodegradation just 6 months after being buried in the soil, while stitched nonwovens retained their mechanical properties even for three growing seasons. In the case of seamed non-woven fabrics, a decrease in selected physical parameters was observed. The addition of wool-based soil additive, a decrease in surface mass was observed by 85% after 6 months, 88% after 18 months and 90% after 30 months. Regardless of the waste materials used, BioWAGs provided plants with continuous access to water and nutrients. The work indicates a sustainable method of managing textile waste and analyzes its properties in the soil, which enables the development of technologies based on natural fibers for applications such as geotextiles, mulches and controlled-release fertilizers. The technology was developed in line with the principles of a circular economy and can improve soil quality, mitigate the effects of climate change and reduce environmental pollution.

Impact of Agroforestry Practices on Soil Microbial Diversity and Nutrient Cycling in Atlantic Rainforest Cocoa Systems

Microorganisms are critical indicators of soil quality due to their essential role in maintaining ecosystem services. However, anthropogenic activities can disrupt the vital metabolic functions of these microorganisms. Considering that soil biology is often underestimated and traditional assessment methods do not capture its complexity, molecular methods can be used to assess soil health more effectively. This study aimed to identify the changes in soil microbial diversity and activity under different cocoa agroforestry systems, specially focusing on taxa and functions associated to carbon and nitrogen cycling. Soils from three different cocoa agroforestry systems, including a newly established agroforestry with green fertilization (GF), rubber (Hevea brasiliensis)–cocoa intercropping (RC), and cocoa plantations under Cabruca (cultivated under the shave of native forest) (CAB) were analyzed and compared using metagenomic and metaproteomic approaches. Samples from surrounding native forest and pasture were used in the comparison, representing natural and anthropomorphic ecosystems. Metagenomic analysis revealed a significant increase in Proteobacteria and Basidiomycota and the genes associated with dissimilatory nitrate reduction in the RC and CAB areas. The green fertilization area showed increased nitrogen cycling activity, demonstrating the success of the practice. In addition, metaproteomic analyses detected enzymes such as dehydrogenases in RC and native forest soils, indicating higher metabolic activity in these soils. These findings underscore the importance of soil management strategies to enhance soil productivity, diversity, and overall soil health. Molecular tools are useful to demonstrate how changes in agricultural practices directly influence the microbial community, affecting soil health.

Urban Soil Dynamics: The Relationship Between Soil Health and Urbanization

Urbanization, combined with unsustainable agricultural practices, climate change, and pollution, poses a significant threat to soil health, leading to degradation with far-reaching impacts on ecosystems and human well-being. This article explores these challenges and specifically focuses on how urbanization affects soil health by degrading its quality and limiting its ability to sustain life. The study examines the dynamics of soil humification, the potential of urban soils to store carbon, and the critical impacts on food production and public health. Through a comprehensive analysis that includes geographical perspectives on soil-related issues, the article highlights innovative management strategies that can enhance the productivity and sustainability of urban soils. It emphasizes the urgent need to integrate soil science into environmental and urban planning to mitigate these negative impacts and promote a sustainable future.

Deciphering the differences of bacterial communities between high- and low-productive wheat fields using high-throughput sequencing.

Microbial communities have been demonstrated to be essential for healthy and productive soil ecosystems. However, an understanding of the relationship between soil microbial community and soil productivity levels is remarkably limited. In this study, bulk soil (BS), rhizosphere soil (RS), and root (R) samples from the historical high-productive (H) and low-productive (L) soil types of wheat in Hebei province of China were collected and analyzed by high-throughput sequencing. The study highlighted the richness, diversity, and structure of bacterial communities, along with the correlation networks among different bacterial genera. Significant differences in the bacterial community structure between samples of different soil types were observed. Compared with the low-productive soil type, the bacterial communities of samples from the high-productive soil type possessed high species richness, low species diversity, complex and stable networks, and a higher relative abundance of beneficial microbes, such as Pseudoxanthomonas, unclassified Vicinamibacteraceae, Lysobacter, Massilia, Pseudomonas, and Bacillus. Further analysis indicated that the differences were mainly driven by soil organic matter (SOM), available nitrogen (AN), and electrical conductivity (EC). Overall, the soil bacterial community is an important factor affecting soil health and crop production, which provides a theoretical basis for the targeted regulation of microbes in low-productivity soil types.

Validation of a new gamma ray soil bulk density sensor

AbstractSoil compaction and soil bulk density are key soil properties affecting soil health and soil ecosystem services like crop production, water retention and purification and carbon sequestration. The standard method for soil bulk density measurements using Kopecky rings is very labour intensive, time consuming and leaves notable damage to the field. Accurate data on bulk density are therefore scarce. To enable large‐scale data collection, we tested a new portable gamma ray sensor (RhoC) for in situ field and dry bulk density measurements up to 1 m depth. In this first validation study, measurements with the RhoC‐sensor were compared with classic ring sampling. Measurements were made in two agricultural fields in the Netherlands (a sandy clay loam and a sandy soil), with large variation in subsoil compaction. At 10 locations within each field, three soil density profiles were made. Each profile comprised six depth measurements (every 10 cm from 10 to 60 cm depth) using the RhoC‐sensor and Kopecky rings, resulting in 30 pairwise profiles and 180 measurements in total per field. At an average soil density of 1.5 g/cm3, the relative uncertainty was 9% for the Kopecky rings and 15% for the RhoC‐sensor. Because the RhoC‐sensor is easy and quick to use, the higher relative uncertainty can easily be compensated for by making additional measurements per location. In conclusion, the RhoC‐sensor allows a reliable quantitative in situ assessment of both field and dry bulk density. This provides the much‐needed possibility for rapid and accurate assessment of soil compaction. The acquisition of this data supports the calculation of soil organic carbon stocks and is indispensable for (national) soil monitoring, to assess soil health and to inform sustainable land management practices for sustained or improved soil health and provision of soil ecosystem services, such as requested in the proposed EU Directive on Soil Monitoring and Resilience.

Modeling the impacts of extreme climate scenarios on soil acidity (pH and exchangeable aluminum) in Abbay River Basin, Ethiopia

The Abbay River Basin faces the looming threat of extreme climate events, including prolonged droughts and erratic rainfall patterns, which can significantly affect soil health and fertility. This study aimed to explore the influence of extreme climate conditions on soil pH and exchangeable aluminum, aiming to promote sustainable agricultural practices in Ethiopia. The Africa Soil Information Service (ASIS) provided datasets on soil pH and exchangeable aluminum. The European Copernicus Climate Change Data Store was used to download historical and future datasets of extreme climatic indices from 1980 to 2010 and 2015–2050, respectively. The Coupled Model Intercomparison Project Phase 6 model ensemble was used to predict future climate impacts under three shared socioeconomic scenarios: SSP1-2.6, SSP2-4.3, and SSP5-8.5. Data extraction, quality control, and clustering were conducted before analysis, and the model was validated for its accuracy and reliability in predicting soil parameter changes. An artificial neural network model was utilized to predict the effects of extreme climate indices on soil pH and exchangeable aluminum concentrations. The model was designed to accurately and reliably predict changes in soil parameters. This study compared the changes in soil pH and aluminum concentrations using paired t tests. The model's diagnostic results indicated a significant impact of extreme climate scenarios on soil pH and exchangeable aluminum. Extreme climate factors such as heavy precipitation and cooler night time temperatures significantly contribute to soil acidification and an increase in aluminum concentration. Under the SSP1-2.6 and SSP2-4.5 emission scenarios, soil pH levels are expected to increase by 8.38 % and 3.79 %, respectively. These changes in soil pH are expected to have significant impacts on the exchangeable aluminum content in the soil, with increases of 37 % and 5.38 %, respectively, under the same emission scenarios. However, the SSP5.8 scenario predicted a 45 % increase in exchangeable aluminum and a 9.36 % decrease in soil pH. Therefore, this study significantly enhances our understanding of the influence of climate change on soil health. The development of strategies to mitigate climate change impacts on agriculture in the region must consider the effects of extreme climate indices.

Soil health indicators in oil palm agroforestry systems in the eastern Amazon, Brazil

Agroforestry systems (AFSs) are generally recognized for improving soil health and increasing soil carbon (C) stock. In the Amazon region, oil palm AFSs have been promoted as a productive strategy and may offer an additional benefit of recovering degraded areas. Our objective was to evaluate whether the management practices adopted in oil palm AFSs affect soil health indicators and C stock. We carried out a study in Tomé-Açu, state of Pará, eastern Amazon, through which we evaluated two oil palm AFSs that differed in species diversity: higher diversity (AFShigh) and lower diversity (AFSlow). We used a regenerating forest (FOR) to compare with the oil palm AFSs, as regenerating forests are also a model of environmental recovery in the eastern Amazon. In the 0–10, 10–20 and 20–30 cm soil layers, we evaluated chemical indicators (active acidity (pH), calcium (Ca), magnesium (Mg), potassium (K), phosphorus (P), and aluminum (Al)), (b) physical indicators (density (SD) and stability of aggregates (AGGRE)), and (c) biological indicators (permanganate oxidizable carbon (POXC), particulate organic carbon (POC), total soil carbon (SOC content) and root stock (ROOT)). We also evaluated the total soil carbon stock (SOC stock). The values of the biological indicators in the most superficial layer, especially the C indicators, were higher in the AFSlow (C content: 1.63 ± 0.13%, POXC: 568 ± 17 mg kg−1, POC: 0.63 ± 0.10 g kg−1) and the AFShigh (C content: 1.84 ± 0.04%, POXC: 656 ± 24 mg kg−1, POC: 0.83 ± 0.06 g kg−1) than in FOR (C content: 1.35 ± 0.09%, POXC: 336 ± 10 mg kg−1, POC: 0.44 ± 0.03 g kg−1). In general, the values of chemical indicators were higher in the AFSs than in FOR, except for the Al content, which was higher in FOR than in the AFSs. The physical indicators did not show a specific pattern of variation between the AFSs and FOR; aggregate stability was higher in FOR (range throughout the soil profile: 5.57 ± 0.09 to 5.38 ± 0.24 mm) than in the AFSlow (5.41 ± 0.03 to 3.79 ± 0.07 mm) and the AFShigh (5.07 ± 0.01 to 3.79 ± 0.11 mm) in all the soil layers, and soil density was higher in the AFShigh and FOR than in the AFSlow in most subsurface layers. Therefore, oil palm AFSs improve the chemical and biological health of the soil, especially in the more superficial layers, but it does not improve the physical health of the soil when compared to the forest.

Feasibility of using bean crop residues for feeding sheep during dry season shortage: implications for economic and environmental resilience

ABSTRACT In Cuba, beans are part of the traditional gastronomy and livelihood of the people. During bean harvest, farmers generate high volumes of stubble, which they burn, thus adversely affecting soil health and organic matter, air quality, and greenhouse gas emissions. Bean harvest occurs during the dry season, a period of feed shortage for livestock, such that integrating farming and livestock production could be a way to feed and fatten sheep at little cost. We assessed the feasibility of feeding bean stubble to sheep by determining 1) volumes produced and nutritional value of stubble, 2) acceptability, performance, and carcass yields of lambs fed stubble, and 3) feasibility for this integrated farming-livestock system at local and regional levels. During harvest, 650 tons of stubble are produced in the municipality of Morón by 190 farmers, which represents a potential to feed more than 3,500 lambs, with acceptable average daily gains of 90 g per day with minimal supplementation. We thus affirm this is a viable technological practice with economic, ecological, and social implications as an acceptable alternative to burning stubble to enhance food security and livelihoods of small and medium-sized farms, while generating new employment opportunities, especially for rural women. Future studies are needed to evaluate the economic, ecological, and social impacts of this agriculture-livestock integration technology.

The effect of four tillage systems on agronomic properties and soil health indicators in southern Manitoba

Soil health encompasses the collective functioning of chemical, physical, and biological properties in soil. The extent to which soil management affects soil health and the links with agronomic outcomes remain unclear. This project aimed to understand the interrelations of tillage systems, soil health, and agronomic properties in Portage la Prairie, MB, Canada. Tillage systems were cultivation, deep tillage, raised beds, and vertical tillage. Soybean ( Glycine max (L.) Merr), corn ( Zea mays L.), and canola ( Brassica napus L.) were all grown in 2020, 2021, and 2022. Crop yield, seed protein content, and seed oil content were measured each year. Soil samples were taken in spring 2021, fall 2021, and fall 2022 and analyzed for nitrate-N, ammonium-N, total N, ACE protein, water extractable organic N, water extractable total N, water extractable ammonium N, soil organic matter, soil organic carbon, calcium carbonate equivalent, CO2 burst, permanganate oxidizable carbon, water extractable organic C, pH, salts, Olsen P, K, S, sand, silt, and clay. Tillage system had a significant impact on agronomic properties in seven crop by sampling combinations. Tillage system effected soil nitrate-N concentration at five crop by sampling combinations, three more than any other soil property. Soybean agronomic properties correlated with soil health indicators more frequently than for corn and canola. This suggests that the utility of soil health indicators may be crop specific. Further research is needed to understand the mechanisms underpinning the ability of soil health indicators to predict agronomic outcomes and to benchmark soil health indicator values with time.

Nutrient management through organic amendments to ensure sustainable and economic cultivation of radish

Radish cultivation heavily relies on fertilizers, adversely affecting soil health. Shifting to organic practices is crucial for sustainability. This study explores combinations of organic amendments (farmyard manure, vermicompost, and poultry manure) to enhance radish growth and yield economically. Ten treatments were tested using a Randomized Block Design. One-way ANOVA, correlation, and regression analyses were conducted. The combination of 50% vermicompost and 50% poultry manure yielded the best results in plant height, leaf growth, fresh and dry weights, and root size. The highest yield (280 q/ha) was achieved with this combination, followed closely by 75% vermicompost and 25% poultry manure (245 q/ha), and 50% farmyard manure with 50% poultry manure (228 q/ha). This combination also proved the most economically and environmentally sustainable, with a benefit-cost ratio of 2.48. Compared to the control, it delivered a gross return 2.44 times higher and a net return 2.85 times higher. Incorporating poultry manure and vermicompost as nutrient sources in radish cultivation is a scientifically sound and economically viable approach, contributing to sustainable agriculture and aligning with UN Sustainable Development Goals.

Exploring the Ecological Implications, Gastronomic Applications, and Nutritional and Therapeutic Potential of Juglans regia L. (Green Walnut): A Comprehensive Review.

The green walnut, which is frequently overlooked in favor of its more mature sibling, is becoming a topic of great significance because of its unique ecological role, culinary flexibility, and therapeutic richness. The investigation of the bioactive substances found in green walnuts and their possible effects on human health has therapeutic potential. Juglans regia L. is an important ecological component that affects soil health, biodiversity, and the overall ecological dynamic in habitats. Comprehending and recording these consequences are essential for environmental management and sustainable land-use strategies. Regarding cuisine, while black walnuts are frequently the main attraction, green walnuts have distinct tastes and textures that are used in a variety of dishes. Culinary innovation and the preservation of cultural food heritage depend on the understanding and exploration of these gastronomic characteristics. Omega-3 fatty acids, antioxidants, vitamins, and minerals are abundant in green walnuts, which have a comprehensive nutritional profile. Walnuts possess a wide range of pharmacological properties, including antioxidant, antibacterial, antiviral, anticancer, anti-inflammatory, and cognitive-function-enhancing properties. Consuming green walnuts as part of one's diet helps with antioxidant defense, cardiovascular health, and general well-being. Juglans regia L., with its distinctive flavor and texture combination, is not only a delicious food but also supports sustainable nutrition practices. This review explores the nutritional and pharmacological properties of green walnuts, which can be further used for studies in various food and pharmaceutical applications.

How Does Specialization in Agricultural Production Affect Soil Health?

The aim of the study was to assess the impact of the specialization of agricultural production on selected parameters of soil health, i.e., soil organic carbon content (SOC), soil acidification, soil nutrient status, i.e., total nitrogen content (NT), available forms of phosphorus, potassium, and magnesium, and microelements content, as well as the content of selected potentially toxic metals (PTMs). For the study, 18 farms located in the Masovian Voivodeship in Central Poland were selected. They were grouped into six types, and each type was represented by three farms. The study included organic farms; farms specializing in: crop, vegetable, poultry, dairy cattle, and pigs production. A total of 144 soil samples were analyzed. The results showed that the specialization of agricultural production and fertilizer management had a significant impact on most of the tested soil health parameters, except SOC and NT content. Despite the high organic fertilizer doses introduced into soils in poultry (170 kg N per hectare as poultry manure) and pig farms (150 kg N per hectare as pig manure), there was no significant influence of these amendments on SOC content. This may indicate low organic carbon sequestration potential in some Polish agricultural soils. Organic farms had the lowest levels of plant nutrients in the tested soil samples, which may limit soil productivity. All the tested soils were strongly acidified, which could restrict both production and regulatory soil functions. Based on the synthetic index of soil fertility (SSFI), vegetable and poultry farms were characterized by very high fertility, while crop, dairy cattle, and pig farms fell into the medium fertility class. Organic farms were in the lowest fertility class. However, the study suggests that the SSFI may not be the best indicator for assessing soil fertility and health; therefore, further research is needed.

A risk entropy approach for linking pesticides and soil bacterial communities

Pesticides play a vital role in ensuring modern agricultural production, but also adversely affecting soil health. Microorganisms are the cornerstone of soil ecology, however, to date, there are few unified standards to measure the risk of soil pesticide residues to soil microbial community. To compensate for this gap, we collected soil samples from 55 orchards and monitored and risk-assessed 165 pesticides to microbial community in the soil. Results showed that a total of 137 pesticides were detected in all samples. Pesticide residues significantly influenced the microbial diversity and community structure in orchard soils, particularly fungicides and herbicides. The risk entropy of each pesticide was calculated in all samples and it was found that 60% of the samples had a "pesticide risk" (Risk quotient > 0.01), where the relative abundance significantly increased in 43 genera and significantly decreased in 111 genera (p < 0.05). Through multiple screens, we finally identified Bacillus and Sphingomonas as the most abundant sensitive genera under pesticide perturbation. The results showed that despite the complexity of the effects of pesticide residues on soils health, we could reveal them by identifying changes in soil bacterial, especially by the differences of microbial biomarkers abundance. The present study could provide new insights into the research strategy for pesticide pollution on soil microbial communities. Environmental ImplicationThe risk of pesticide residues in soil needs to be quantified and standardized. We believe that microorganisms can be used as a marker to indicate soil pesticide residue risk.For this end, we investigated the residues of 165 pesticides in 55 orchard soil samples, calculated pesticide risk entropy and their effects on the soil microbial community.Through multiple analyzing and screening, we ultimately identified that, out of the 154 detected biomarkers, Bacillus and Sphingomonas were the most abundant sensitive genera under pesticide perturbation, which have the potential to be used as key biomarkers of soil microbiomes induced by pesticide perturbation.

Quantitative Assessment of the Effect of Agency-Led Prescribed Burns and Cultural Burns on Soil Properties in Southeastern Australia

Fire management techniques play a critical role in mitigating the impact of bushfires on communities and ecosystems. In Australia, government agencies implement hazard reduction burn programs, while Indigenous communities have used fire for ecosystem management for thousands of years. The positive effect of prescribed burning goes beyond bushfire risk mitigation, with impacts also on soil and ecosystem health. This study evaluates the effects of prescribed burning on soil properties, with implications for soil and ecosystem health. Two fire management techniques were evaluated: agency-led prescribed burning and cultural burning. Both fire treatments resulted in an increase in soil moisture, showing that they positively affect the soil water balance (the greater effect seen following the agency-led burn). Both fire treatments also resulted in a decrease in soil bulk density and an increase in organic matter content, with the greater effect seen for soils affected by the Indigenous-led burn. These results show that both fire management techniques positively affect soil health, with important consequences for aboveground ecosystem health. Cultural burning is the most efficient to promote reduced soil bulk density (important for nutrient availability and microbial activity) and increase carbon and nitrogen stores.

Abundance, diversity and distribution of soil nematodes in Kangaita and Weru tea catchments of Kirinyaga and Tharaka Nithi counties, Kenya

Nematodes are the most abundant animals on earth and play essential roles in ecosystem functioning hence their abundance and diversity affect soil health. Nematodes have been reported in tea fields in some parts of Kenya and previous studies indicate that they may be a cause for the decline of tea population in some tea fields in Kenya. Nematodes of Moloidogyne spp have also been reported to be responsible for death of tea plants in nursery conditions. A survey was carried out in Weru and Kangaita tea factories catchment areas in Tharaka Nithi and Kirinyaga counties respectively. The survey aimed to determine the abundance and diversity of nematodes in small holder tea farms. Kangaita represented the high elevation site while Weru represented the low elevation site. Soil samples were collected from smallholder tea farms from which nematodes were extracted, identified based on their morphological characteristics, and classified according to their feeding habits then quantified using standard protocols. Nematodes from 23 genera were recovered in the two study sites representing all the five feeding groups: plant feeders, fungal feeders, bacterial feeders, omnivores, and predatory nematodes. Of the 23 genera, 11 were plant feeders, 6 bacterial feeders, 3 fungal feeders 2 omnivores and 1 predatory nematode. Kangaita, being a high elevation site reported higher population density in most genera reported than Weru which is a low elevation site. This is a departure from most studies that have reported higher nematode population densities in low altitudes. This can be attributed to differences in climatic and soil conditions in the two study sites in the same season. Kangaita was cooler with deep, well-ventilated, and loose soils while Weru was hotter with mostly compacted, shallow, and poorer soils in the tea farms. There is need for further research on the effect of elevation and farming practices on the distribution, abundance, and diversity of nematodes in tea fields. Key words: Nematodes, abundance, soil health, tea fields, elevation, diversity, feeding group

Impacts of biostimulants on crop yield and biological activity under drought conditions

AbstractIntroductionBiostimulants are proposed to have a role in sustainable food production and are being increasingly used strategies to limit the negative effects of drought stress on crop yield and soil health. However, how different biostimulants used alone, or in combination with conventional management approaches affect soil health and crop yield under drought in different soils is not well understood. Here we conducted a glasshouse experiment to investigate this.Materials and MethodsTwo soil types from fields maintained under intensive (IM) and extensive (EM) management practices were used with two commercial biostimulants [Universal Natural Plant food (UNP) and Converte Seed Primer (CSP)] on soil microbial populations and crop yield (lettuce) under well‐watered and drought stressed conditions. We examined the activity and biomass of soil microorganisms as well as seed germination and root and shoot biomass to examine the effect of application of biostimulants on parameters of soil health and crop yield.ResultsBiostimulants generally increased crop yield, basal soil respiration and microbial biomass, with effects stronger in EM soils than in IM soils, but effects on soil enzyme activities were variable. The combined use of biostimulant and inorganic fertiliser negated the benefits of the biostimulant on soil biological properties in some cases. Nevertheless, effects of biostimulants had a positive impact on crop yield with the combined use of soil‐applied and seed applied biostimulants have the greatest impact on plant biomass and also conferring some resistance in both soil microbial communities and plant growth to drought.ConclusionsWhile biomass and activity of the microbial community and plant growth responses to biostimulant additions are both soil and biostimulant dependent, their combined use has potential to aid both plants and maintain promote microbial activity under of drought, compared to conventional fertiliser treatments in extensively managed soils.

Combined application of up to ten pesticides decreases key soil processes

Natural systems are under increasing pressure by a range of anthropogenic global change factors. Pesticides represent a nearly ubiquitously occurring global change factor and have the potential to affect soil functions. Currently the use of synthetic pesticides is at an all-time high with over 400 active ingredients being utilized in the EU alone, with dozens of these pesticides occurring concurrently in soil. However, we presently do not understand the impacts of the potential interaction of multiple pesticides when applied simultaneously. Using soil collected from a local grassland, we utilize soil microcosms to examine the role of both rate of change and number of a selection of ten currently used pesticides on soil processes, including litter decomposition, water stable aggregates, aggregate size, soil pH, and EC. Additionally, we used null models to enrich our analyses to examine potential patterns caused by interactions between pesticide treatments. We find that both gradual and abrupt pesticide application have negative consequences for soil processes. Notably, pesticide number plays a significant role in affecting soil health. Null models also reveal potential synergistic behavior between pesticides which can further their consequences on soil processes. Our research highlights the complex impacts of pesticides, and the need for environmental policy to address the threats posed by pesticides.

Erosion Hazard Level in Jenelata Watershed, Gowa Regency, South Sulawesi, Indonesia Based on RUSLE Model

Erosion is the main problem that affects soil health related to agricultural activities, therefore this study aims to determine the level of erosion hazard in the Jenelata Sub Watershed. RUSLE is used to calculate erosion prediction using rainfall erosion information, soil erodibility value, topographic value and using maps for vegetation and conservation practices, so that erosion values are obtained for Buakkang, Bissoloro, Bontomanai, Jenebatu, Sapaya Village, Paranglompoa, Pattalikang, Tassese, Mangempeng, Paladigan, and Ronaloe. Each was divided into very low classes with land loss of less than 15 t/ha/year with a land area of 7812.38 ha. The low class was land loss of 15 to 60 t/ha/year with a land area of 3263.04 ha. The medium class was land loss of 60 to 180 t/ha/year with a land area of 694.76 ha. The high class was land loss of 180 to 480 t/ha/year with a land area of 3234.03 ha, and the very high class was land loss that is greater than 480 t/ha/year with a land area of 5272.67 ha. This study showed high and very high erosion with a land area of 3234.03 ha and 5272.67 ha and very low erosion with a land area of 7812.38 ha.

Investigating the toxic effects of ethoprophos on Eisenia fetida: Integrating light microscopy, scanning electron microscopy, and biochemical analysis

This research investigated the ecological impact of exposing Eisenia fetida, an essential component of soil ecosystems, to the organophosphate pesticide ethoprophos, widely used in agriculture. With a focus on understanding the specific effects on earthworms, we employed three concentrations (7.5, 15, and 30 mg/kg) over 28 days, considering the pesticide's short half-life and existing data on environmental concentrations. We aimed to contribute to a broader understanding of how these pesticides affect soil health. Histological analysis, including staining with Hematoxylin-eosin, Mallory Trichrome, Periodic acid-Schiff, and Alcian blue methods, was conducted on control and treatment groups. The histological and histopathological results were evaluated using the light microscopy, revealing various degenerations in the epithelial and muscle layers. Scanning electron microscopy analysis detected concentration-related notable compaction of the body surface, asymmetry, and distortion in the body segments. In the exposed groups, especially those subjected to higher ethoprophos concentrations, the grid-like appearance of the clitellum was visibly disturbed. This disturbance in the grid-like pattern is indicative of structural changes and disruptions at the microscopic level. Furthermore, total protein, carbohydrate, lipid analyses, as well as acid phosphatase and alkaline phosphatase enzyme activities, were also evaluated for earthworms from each experimental group. The analyses showed a concentration-related decrease in all biochemical measurements, except acid phosphatase enzyme activity. In conclusion, our study reveals that the environmentally realistic concentrations of ethoprophos, an effective and widely used pesticide in pest control, have detrimental effects on the health and physiology of E. fetida. These effects are manifested through histological deformities, altered biochemical profiles, and observable physiological disturbances. These results shed light on the harmful effects of ethoprophos on earthworms, underlining the necessity to restrict its usage in agricultural practices and thereby support environmental sustainability.