In the various car garages of Kinshasa (in the Democratic Republic of Congo), the lack of consideration of environmental standards is the basis of the voluntary and/or accidental discharges of used oils directly into the environment without any prior treatment. These used hydrocarbons are pollutants that can cause major environmental problems due to their toxicity. The aim of this study is to treat used lubricating oils from car garages biologically in order to reduce their toxic potential. To this end, an aerobic biological treatment system was set up by mixing 4L of used oils with IL of urine, 2Kg of active Kaolin and 20L of water. The reduction in the toxicity of hydrocarbons was assessed through acute toxicity tests on the species Gambusia Affinis and microbiological analyses before and after biological treatment of these oils. The results obtained on the toxicity before treatment of these oils revealed that the LC50 was around ±3 ml/g of used oils to say that the pollutant is very toxic. After biological treatment, the LC50 rise to around ±66.7 ml/g for the treated oils, which represents a significant reduction in toxicity and ranks these treated oils in the order of less toxic effluents. The microbiological analyses of the used oils before their treatment did not show any bacteria; on the other hand, the analyses after treatment made it possible to highlight the presence of bacterial colonies whose identification revealed the following bacteria: Citrobacter, Enterobacter, Klebsiella and Pseudomonas, which are mostly lipolytic. This study thus allowed to treat used oils thanks to microorganisms and the intrinsic properties of urine and Kaolin.
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