Aerobic Granular Sludge System Research Articles

The impacts of biodegradable and non-biodegradable microplastic on the performance and microbial community characterization of aerobic granular sludge

IntroductionMicroplastics (MPs), identified as emerging contaminants, have been detected across diverse environmental media. Their enduring presence and small size facilitate the adsorption of organic pollutants and heavy metals, leading to combined pollution effects. MPs also accumulate in the food chain thus pose risks to animals, plants, and human health, garnering significant scholarly attention in recent years. Aerobic granular sludge (AGS) technology emerges as an innovative approach to wastewater treatment. However, the impacts of MPs on the operational efficiency and microbial characteristics of AGS systems has been insufficiently explored.MethodsThis study investigated the effects of varying concentration (10, 50, and 100 mg/L) of biodegradable MPs (Polylactic Acid, PLA) and non-biodegradable MPs (Polyethylene Terephthalate, PET) on the properties of AGS and explored the underlying mechanisms.Results and discussionsIt was discovered that low and medium concentration of MPs (10 and 50 mg/L) showed no significant effects on COD removal by AGS, but high concentration (100 mg/L) of MPs markedly diminished the ability to remove COD of AGS, by blocking most of the nutrient transport channels of AGS. However, both PLA and PE promoted the nitrogen and phosphorus removal ability of AGS, and significantly increased the removal efficiency of total inorganic nitrogen (TIN) and total phosphorus (TP) at stages II and III (P < 0.05). High concentration of MPs inhibited the growth of sludge. PET noticeably deteriorate the sedimentation performance of AGS, while 50 mg/L PLA proved to be beneficial to sludge sedimentation at stage II. The addition of MPs promoted the abundance of Candidatus_Competibacter and Acinetobacter in AGS, thereby promoting the phosphorus removal capacity of AGS. Both 50 mg/L PET and 100 mg/L PLA caused large amount of white Thiothrix filamentous bacteria forming on the surface of AGS, leading to deterioration of the sludge settling performance and affecting the normal operation of the reactor. Comparing with PET, AGS proved to be more resistant to PLA, so more attention should be paid to the effect of non-biodegradable MPs on AGS in the future.

Rapid Formation and Performance of Aerobic Granular Sludge Driven by a Sodium Alginate Nucleus under Different Organic Loading Rates and C/N Ratios

The use of aerobic granular sludge (AGS) for wastewater treatment has emerged as a promising biotechnology. A sodium alginate nucleus (SAN) incorporated into the AGS system can enhance aerobic granulation. Two important parameters influencing AGS formation and stability are the organic loading rate (OLR) and C/N ratio. In this study, AGS containing the SAN was cultivated under different OLR and C/N ratios. Through morphological analysis, physicochemical properties, and water quality analysis, the effects of the OLR and C/N ratio on the rapid formation and performance of AGS containing the SAN were investigated. The results showed that the most suitable OLR and C/N ratio in the SAN system were 1.4–2.4 kg/(m3∙d) and 10–15, respectively. A recovery experiment of sodium alginate (SA) showed that the group that formed AGS generally had a higher recovery efficiency compared with the group that did not form granular sludge. This work explored the suitable granulation conditions of AGS containing the SAN, and the results provide a theoretical basis for future practical applications. The recycling of SA as presented in this study may broaden the application prospects of SA.

Effects of salinity on glycerol conversion and biological phosphorus removal by aerobic granular sludge

Industrial wastewater often has high levels of salt, either due to seawater or e.g. sodium chloride (NaCl) usage in the processing. Previous work indicated that aerobic granular sludge (AGS) is differently affected by seawater or saline water at similar osmotic strength. Here we investigate in more detail the impact of NaCl concentrations and seawater on the granulation and conversion processes for AGS wastewater treatment. Glycerol was used as the carbon source since it is regularly present in industrial wastewaters, and to allow the evaluation of microbial interactions that better reflect real conditions. Long-term experiments were performed to evaluate and compare the effect of salinity on granulation, anaerobic conversions, phosphate removal, and the microbial community. Smooth and stable granules as well as enhanced biological phosphorus removal (EBPR) were achieved up to 20 g/L NaCl or when using seawater. However, at NaCl levels comparable to seawater strength (30 g/L) incomplete anaerobic glycerol uptake and aerobic phosphate uptake were observed, the effluent turbidity increased, and filamentous granules began to appear. The latter is likely due to the direct aerobic growth on the leftover substrate after the anaerobic feeding period. In all reactor conditions, except the reactor with 30 g/L NaCl, Ca. Accumulibacter was the dominant microorganism. In the reactor with 30 g/L NaCl, the relative abundance of Ca. Accumulibacter decreased to ≤1 % and an increase in the genus Zoogloea was observed. Throughout all reactor conditions, Tessaracoccus and Micropruina, both actinobacteria, were present which were likely responsible for the anaerobic conversion of glycerol into volatile fatty acids. None of the glycerol metabolizing proteins were detected in Ca. Accumulibacter which supports previous findings that glycerol can not be directly utilized by Ca. Accumulibacter. The proteome profile of the dominant taxa was analysed and the results are further discussed. The exposure of salt-adapted biomass to hypo-osmotic conditions led to significant trehalose and PO43−-P release which can be related to the osmoregulation of the cells. Overall, this study provides insights into the effect of salt on the operation and stability of the EBPR and AGS processes. The findings suggest that maintaining a balanced cation ratio is likely to be more important for the operational stability of EBPR and AGS systems than absolute salt concentrations.

Aerobic granulation and bioresource production under intermittent saline stress

Aerobic Granular Sludge (AGS) systems have gained prominence for liquid waste treatment and bioresource recovery. In this regard, various strategies are employed to accelerate granulation processes in sequencing batch reactors (SBRs) and maximize stability and bioresource production. Intermittent stress supplementation can facilitate granule development, promoting slow-growth microorganisms. At the same time, osmotic stress may enhance bioresource production. In this study, an AGS reactor control with no saline stress (R1) was compared to two AGS systems operated under constant (R2) and intermittent (R3) saline stress in terms of efficiency, stability and bioresource productions (alginate-like exopolysaccharides-ALE and tryptophan). Granulation occurred around day 59 and 21 in R2 and R3, respectively, while complete granulation was not achieved in R1 within the 80-day operational period. However, SBRs performed well after 45 days, with 70% of the biomass being granular. The production of the biopolymer ALE remained more stable in reactors with osmotic stress compared to R1. The microbiological analysis revealed specific responses and adaptations to environmental factors, with genera such as Thauera and Pseudofulvimonas showing higher abundance in reactors with complete granulation, indicating a positive correlation with saline conditions (5 g/L). These insights are crucial for optimizing the performance of AGS systems in future applications, emphasizing the benefits of intermittent saline stress in promoting rapid granulation, stability in bioresource production, contaminant removal, and robust granule formation.

Aerobic granulation and resource production under continuous and intermittent saline stress

Three sequential batch reactors (SBR) were operated to evaluate salt addition’s impact on granulation, performance, and biopolymer production in aerobic granular sludge (AGS) systems. System R1 was fed without adding salt (control); system R2 operated with saline pulses, i.e., one cycle with salt (2.5 g NaCl/L) addition followed by another without salt; and R3 received continuous supplementation of 2.5 g NaCl/L. The results indicated that the reactors supplemented with salt presented higher concentrations of mixed liquor volatile suspended solids (MLVSS) and better settleability than R1, showing that osmotic pressure contributed to biomass growth, accelerated granulation, and improved physical characteristics. The faster granulation occurred in R2, thus proving the beneficial effects of intermittent salt addition through alternating pulses. Salt addition did not impair the simultaneous removal of carbon, nitrogen, and phosphorus. In fact, R2 showed better carbon removals. In conclusion, continuous or intermittent (pulsed) supplementation of 2.5 g NaCl/L did not lead to increased production of extracellular polymeric substances (EPS) and alginate-like exopolymers (ALE). This outcome could be attributed to the low saline concentration employed, a higher food-to-microorganism (F/M) ratio observed in R1, and possibly greater endogenous consumption of biopolymers in the famine period in R2 and R3 due to the greater solids retention time (SRT). Therefore, this study brings important results that contribute to a better understanding of the effect of salt in continuous dosing or in pulses as a selection pressure strategy to accelerate granulation, as well as the behavior of the AGS systems for saline effluents.

Insight into the role of chitosan in rapid recovery and re-stabilization of disintegrated aerobic granular sludge

The disintegration and instability of aerobic granular sludge (AGS) systems during long-term operation pose significant challenges to its practical implementation, and rapid recovery strategies for disintegrated AGS are gaining more attention. In this study, the recovery and re-stabilization of disintegrated AGS was investigated by adding chitosan to a sequencing batch reactor and simultaneously adjusting the pH to slightly acidic condition. Within 7 days, chitosan addition under slight acidity led to the re-aggregation of disintegrated granules, increasing the average particle size from 166.4 μm to 485.9 μm. Notably, sludge volume indexes at 5 min (SVI5) and 30 min (SVI30) decreased remarkably from 404.6 mL/g and 215.1 mL/g (SVI30/SVI5 = 0.53) to 49.1 mL/g and 47.6 mL/g (SVI30/SVI5 = 0.97), respectively. Subsequent operation for 43 days successfully re-stabilized previous collapsed AGS system, resulting in an average particle size of 750.2 μm. These mature and re-stabilized granules exhibited characteristics of large particle size, excellent settleability, compact structure, and high biomass retention. Furthermore, chitosan facilitated the recovery of COD and nitrogen removal performances within 17–23 days of operation. It effectively facilitated the rapid aggregation of disintegrated granules by charge neutralization and bridging effects under a slightly acidic environment. Moreover, the precipitated chitosan acted as carriers, promoting the adhesion of microorganisms once pH control was discontinued. The results of batch tests and microbial community analysis confirmed that chitosan addition increased sludge retention time, enriching slow-growing microorganisms and enhancing the stability and pollutant removal efficiency of the AGS system.

Aerobic granulation in a continuous-flow simultaneous nitrification, endogenous denitrification, and phosphorus removal system fed with low-strength wastewater: Granulation mechanism and microbial succession

The culture of aerobic granular sludge (AGS) in continuous-flow system for treating low-strength municipal wastewater is still a challenge for its widespread application. This study developed a continuous-flow reactor based on metabolic and hydraulic selection pressure to explore the feasibility of culturing AGS in conventional continuous-flow mode. The results showed that aerobic granulation was successfully achieved with a mean particle size of 373 μm and almost all granules (95.6 %) were larger than 200 μm. An obvious three-stages granulation process comprised of initial adhesion, the formation of early aggregates that acted as nuclei of granules, and the final AGS maturation, was observed in this work. The selective enrichment of slow-growing bacteria in alternating feast/famine condition played a critical role in the aerobic granulation. The analysis results of extracellular polymeric substances revealed that β-polysaccharides and proteins distributed uniformly within the mature granules, while α-polysaccharides were found mainly concentrated around the granules, which was believed to enhance the mechanical strength of granules. The continuous-flow AGS system exhibited an excellent simultaneous carbon, nitrogen, and phosphorus removal performance with high removal efficiencies of NH4+-N (97.6 %), TIN (91.0 %), COD (93.7 %), and TP (94.8 %). Moreover, a low sludge yield of 0.1 gMLSS/gCOD was obtained for dramatic in situ sludge reduction. Illumina MiSeq sequencing results confirmed the outstanding accumulation of GAOs (Candidatus_Competibacter) and PAOs (Rhodocyclaceae) in the system, which were responsible for simultaneous C, N, P removal and sludge reduction. Overall, this study demonstrated the feasibility of aerobic granulation in conventional continuous-flow mode and offered insights into the granulation mechanism, providing implications and references for the application of AGS technology in existing wastewater treatment plants.

Comparative evaluation of activated sludge and aerobic granular sludge for biological treatment of real domestic wastewater with oxytetracycline dosing

Aerobic granular sludge technology is an emerging choice for effective and sustainable wastewater treatment. The aim of this study was to determine the effect of antibiotics such as oxytetracycline on biological nutrient removal pathways in aerobic granular sludge and activated sludge sequencing batch reactors treating real domestic wastewater. The aerobic granular sludge and activated sludge reactors were operated in parallel for evaluating treatment of domestic wastewater and its response to 50–300 µg/L oxytetracycline dosing. The aerobic granular sludge and activated sludge sequencing batch reactors were dominated by compact granules (sludge volume index (SVI): 60 mL/g) and flocs (SVI: >100 mL/g), respectively, during treatment of real wastewater. Aerobic granular sludge has significantly outperformed activated sludge in achieving high phosphate removal of ∼99% against just 57%. This was corroborated with higher abundance of polyphosphate accumulating organisms and enhanced bio-P removal in the aerobic granular sludge system. In contrast, phosphate removal was achieved through normal bio-P removal in the activated sludge reactor. Oxytetracycline removals were distinct at 90% and 23%, respectively, in the aerobic granular sludge and activated sludge systems. High total nitrogen and phosphate removals of 97% and 96%, respectively, were maintained in the aerobic granular sludge system even during OTC dosing. In contrast, the total nitrogen and phosphate removals have dropped to 78% and 16%, respectively, in the activated sludge system due to oxytetracycline stress. Efficient biological nutrient removal and process stability along with environmental sustainability are critical for choosing aerobic granular sludge technology in wastewater treatment plants.

Coupling micro-electric field into aerobic granular sludge system for sulfadiazine abatement: Performance, mechanism, toxicity, and microbial characteristics

The residual antibiotics in water has aroused ever-growing concern of people. Aerobic granular sludge (AGS) technology shows great application potential for removing toxic organics from wastewater due to its unique features. However, it is still hindered by some bottleneck problems, such as a long culture period and instability of granular sludge. Here, micro-electric field (MEF) coupled AGS (e-AGS) system was established to solve the problems. MEF significantly shortened the cultivation period of AGS (25 days in e-AGS vs. 30 days in control), which mainly related to the production of extracellular polymers. The granular sludge exposed to MEF exhibited a smaller size (3.4 mm), a more compact structure, and high removal rates for sulfadiazine (SDZ, 65 %), ammonia nitrogen (96.49 %), chemical oxygen demand (91.28 %), and total phosphorus (89.31 %). SDZ degradation intermediates and pathways in the e-AGS system were revealed. The eco-toxicity of the effluent was evaluated based on the eco-toxicity of SDZ transformation products. Microbes succession suggested that MEF functioned in a selective role for microorganisms’ growth. At the genus level, Acinetobacter, Bdellovibrio, Leadbetterella, and Aeromonas dominated the degradation of SDZ. This work provides an effective means for the enhancement of the AGS system to treat antibiotic-polluted water and makes provision for the application of AGS.

Enhanced granulation of activated sludge in an airlift reactor for organic carbon removal and ammonia retention from industrial fermentation wastewater: A comparative study

Ammonia retention and recovery from high-nitrogenous wastewater are new concepts being used for nitrogen management. A microaerophilic activated sludge system was developed to convert organic nitrogen into ammonia and retain it for its recovery; however, the settleability of activated sludge remains a challenge. Therefore, this study proposed an aerobic granular sludge system as a potential solution. Two types of sequencing batch reactors—airlift and upflow reactors—were operated to investigate the feasibility of fast granule formation, the performance of organic carbon removal and ammonia retention, and the dynamics of microbial community composition. The operation fed with industrial fermentation wastewater demonstrated that the airlift reactor ensured a more rapid granule formation than the upflow reactor because of the high shear force, and it maintained a superior ammonia retention stability of approximately 85 %. Throughout the operational period, changes in hydraulic retention time (HRT), settling time, and exchange ratio altered the granular particle sizes and microbial community compositions. Rhodocyclaceae were replaced with Comamonadaceae, Methylophilaceae, Xanthomonadaceae, and Chitinophagaceae as core taxa instrumental in granulation, likely because of their extracellular polymeric substance secretion. As the granulation process progressed, a significant decrease in the relative abundances of nitrifying bacteria—Nitrospiraceae and Nitrosomonadaceae—was observed. The reduction of settling time and HRT enhanced granulation and inhibited the activity of nitrifying bacteria. The success in granulation for ammonia conversion and retention in this study accelerates the paradigm shift from ammonia removal to ammonia recovery from industrial fermentation wastewater.

Smaller Aerobic Granules Significantly Reduce N2O Production by Ammonia-Oxidizing Bacteria: Evidences from Biochemical and Isotopic Analyses.

The mitigation of nitrous oxide (N2O) is of primary significance to offset carbon footprints in aerobic granular sludge (AGS) systems. However, a significant knowledge gap still exists regarding the N2O production mechanism and its pathway contribution. To address this issue, the impact of varying granule sizes, dissolved oxygen (DO), and nitrite (NO2-) levels on N2O production by ammonia-oxidizing bacteria (AOB) during nitrification in AGS systems was comprehensively investigated. Biochemical and isotopic experiments revealed that increasing DO or decreasing NO2- levels reduced N2O emission factors (by 13.8 or 19.5%) and production rates (by 0.08 or 0.35 mg/g VSS/h) via weakening the role of the AOB denitrification pathway since increasing DO competed for more electrons required for AOB denitrification. Smaller granules (0.5 mm) preferred to diminish N2O production via enhancing the role of NH2OH pathway (i.e., 59.4-100% in the absence of NO2-), while larger granules (2.0 mm) induced conspicuously higher N2O production via the AOB denitrification pathway (approximately 100% at higher NO2- levels). Nitrifying AGS systems with a unified size of 0.5 mm achieved 42% N2O footprint reduction compared with the system with mixed sizes (0.5-2.0 mm) under optimal conditions (DO = 3.0 mg-O2/L and NO2- = 0 mg-N/L).

Novel insights into the impact of anticancer drugs on the performance and microbial communities of a continuous-flow aerobic granular sludge system

Anticancer drugs are frequently found in domestic wastewater, but knowledge of their impacts on wastewater treatment processes is limited. The effects of three levels of concentrations (low, medium, and high) of three anticancer drugs on physicochemical parameters and prokaryotic communities of a continuous-flow aerobic granular sludge (AGS) system were examined. Drugs at medium and high concentrations reduced the removal of total nitrogen and organic matter during the first 15 days of operation by approximately 15–20 % compared to a control, but these effects disappeared afterward. Removal efficiencies of drugs were in the range of 51.2–100 % depending on the concentration level. Drugs at medium and high concentrations reduced the abundance and diversity and altered the composition of prokaryotic communities. Specific taxa were linked to variations in performance parameters after the addition of the drugs. This study provides improved knowledge of the impacts of anticancer drugs in AGS systems operated in continuous-flow reactor.

Disintegration and re-granulation of aged algal-bacterial aerobic granular sludge in wastewater treatment

Long-term stability in practical applications is one of the crucial issues challenging the sustainable operation of aerobic granular sludge (AGS)-based wastewater treatment systems. Numerous attempts have been made to improve its long-term stability, among which introducing microalgae to bacterial AGS systems seems promising. This study investigated and recorded the whole process of disintegration and re-granulation of the aged algal-bacterial AGS in a 42-day operation. The disintegration of granules was signaled by broken structure and more effluent biomass discharge, resulting in deterioration of phosphorus removal from 86 % on day 1 to 7 % on day 20 and some slight decrease in chlorophyll a content. The broken granules were found to be filamentous flocs or granule fragments, and the latter with coexisting microalgae possibly accounted for the re-granulation and system recovery. Large proportions of fragments could be developed into new granules on day 30 under no additional strategy adoption. And the effluent biomass, chlorophyll a content and phosphorus removal can recover to their initial levels within 34 days' operation. Interestingly, organics and ammonia removals were not affected, which kept around 99 % during the whole test period. Results from this study provide a more in-depth understanding of the stability of algal-bacterial AGS and its long-term operation management.

Effect of in situ ultrasonic wave and influent ammonia nitrogen fluctuation on stability of aerobic granular sludge

ABSTRACT This study elucidates the impact of fluctuating influent conditions and in situ ultrasonic wave exposure on the stability of aerobic granular sludge (AGS) in the treatment of simulated wastewater emanating from rare earth mining operations. During a stable influent period spanning from Day 1 to Day 95, the seed granules underwent an initial disintegration followed by a re-granulation phase. The secondary granulation was achieved on Day 80 and Day 40 for the ultrasonic reactor (R1) and the control reactor (R2), respectively. Notably, granules formed in R1 exhibited a more porous structure compared to those generated in R2. Subsequently, when the ammonia nitrogen in the influent oscillated between 100 and 500 mg/L during Days 96-140, both reactors yielded compact and densely structured granules. Nitrogen removal profiles were comparable between the two reactors: the removal efficiencies for ammonia nitrogen and total inorganic nitrogen escalated from 95% and 80%, respectively, during Days 1-95, to 95% and 90%, respectively, post-Day 140. A suite of performance metrics indicated that steady-state granules from R1 outperformed those from R2 across several parameters. Specifically, the nitrification/denitrification rates, and relative abundance of denitrifying bacteria were all higher in granules from R1. Conversely, the relative abundance of nitrifying bacteria was comparable between granules from both reactors. However, R1 granules demonstrated lower sludge concentration and smaller average particle size than their R2 counterparts. In conclusion, the AGS system demonstrated robust resilience to fluctuating ammonia nitrogen, and the application of ultrasonic waves significantly enhanced granular activity while achieving in situ sludge reduction.

Comprehensive analysis reveals the differentiated influential mechanism of degradable poly (ε-caprolactone) and polybutylene succinate microplastics on nitrogen transformation in aerobic granular sludge systems

The escalating releasing of degradable microplastics (DMPs) into wastewater may pose a potential threat to biological nitrogen removal. However, whether and how different DMPs affect nitrogen transformations in aerobic granular sludge (AGS) systems remains unclear. This work therefore aimed to fill such knowledge gaps by employing poly (ε-caprolactone) (PCL) and polybutylene succinate (PBS) as the model microplastics. Results revealed that exposure to 0.5 and 5 mg/L DMPs did not remarkably affect the nitrogen removal. However, increasing DMPs concentration to 50 mg/L suppressed the nitrogen transformation, causing a significant degree of inhibition in total nitrogen removal efficiency, particularly in the PCL-exposure group (decreased by 2.0%–25.7%). The pronounced inhibitory effects were attributed to the more readily biodegradability of PCL compared to PBS, resulting in an enhanced release of microplastics that induced excessive production of reactive oxygen species and destruction of cytomembrane integrity. Consequently, the relative abundance of denitrifiers was decreased, leading to suppressed expression of the genes (e.g. narG) involved in nitrogen transformation. This was accompanied by a decline in enzyme activity (e.g. nitrate reductase) and electron transport system activity. The correlations among genes, microbes and environmental factors were reshaped by different DMPs, underlying the intrinsic mechanism for the deteriorated nitrogen removal. Overall, this study implies that compared to PBS microplastics, the accumulation of PCL microplastics in aquatic environments poses a more significant threat to biological treatment efficacy and global nitrogen cycling, thereby warranting increased attention.

Effect of C/N on the microbial interactions of aerobic granular sludge system

The main focus of this study was to evaluate the operational stability and changes in microbial interactions of aerobic granular sludge (AGS) systems at reduced C/N (16, 8 and 4). The results showed that the removal efficiency of total nitrogen and total phosphorus decreased from 95.99 ± 0.93% and 84.44 ± 0.67% to 48.46 ± 1.92% and 50.93 ± 2.67%, respectively, when C/N was reduced from 16 to 4. The granule settling performance and stability also deteriorated. Molecular ecological network analysis showed that the reduction of the C/N ratio made the overall network as well as the subnetworks of the Proteobacteria and Bacteroidota more complex and tightly connected. Similarly, the subnetworks of two dominant genera (Thiothrix and Defluviicoccus) became more complex as the C/N decreased. Meanwhile, the decreased C/N ratio might promote competition among microbes in these overall networks and subnetworks. In conclusion, reduced C/N added complexity and tightness to microbial linkages within the AGS system, while increased competition between species might have contributed to the deterioration in pollutant removal performance. This study adds a new dimension to our understanding of the effects of C/N on the microbial community of AGS using a molecular ecological network approach.

Feast/famine ratio regulates the succession of heterotrophic nitrification-aerobic denitrification and autotrophic ammonia oxidizing bacteria in halophilic aerobic granular sludge treating saline wastewater

Heterotrophic nitrification-aerobic denitrification (HN-AD) shows innovation potential of wastewater treatment process in a single tank. However, how to enrich HN-AD bacteria in activated sludge to enhance their contribution remained unknown. This study explored the impact of the feast/famine (F/F) ratio on the succession of autotrophic ammonia oxidizing bacteria (AOB) and HN-AD bacteria in a halophilic aerobic granular sludge (HAGS) system. As the F/F ratio decreased from 1/9 to 1/15, the total inorganic nitrogen (TIN) removal performance significantly decreased. The proportion of heterotrophic bacteria was dropped from 79.0 % to 33 %. Accordingly, the relative abundance of Paracoccus decreased from 70.8 % to 25.4 %, and the copy number of the napA gene was reduced from 2.2 × 1010 copies/g HAGS to 8.1 × 109 copies/g HAGS. It found the F/F ratio regulated the population succession of autotrophic AOB and HN-AD bacteria, thereby providing a solution to achieve the enrichment of HN-AD bacteria in HAGS.

Overlooked role in bacterial assembly of different-sized granules in same sequencing batch reactor: Insights into bacterial niche of nutrient removal

The unique ecosystem within different-sized granules affects microbial assembly, which is crucial for wastewater treatment performance. This study operated an aerobic granular sludge system to evaluate its performance in treating synthetic municipal wastewater. Subsequently, the microbial community within different-sized granules was characterized to investigate bacterial assembly, and elucidated their biological potential for nutrient removal. The nutrient removal efficiencies were as follows: 93.8 ± 2.8 % chemical oxygen demand, 65.4 ± 4.0 % total nitrogen, and 93.8 ± 6.8 % total phosphorus. The analysis of microbial assembly unveiled remarkable diversity among different-sized sludges, the genus relative abundance displayed 61.4 % positive and 33.0 % negative correlation with sludge size. The excellent potential for organic degradation, denitrification, and polyphosphate accumulation occurred in sludge sizes of > 0.75 mm, 0.20–0.50 mm, and < 0.20 mm, respectively. Functional annotation further confirmed the nutrient removal potential within different-sized sludges. This study provides valuable insights into the bacterial niche of different-sized sludges, which can enhance their practical application.

Preparation of hydroxyapatite (HAP) from waste eggshells for enhancing the granulation and treatment performance of aerobic granular sludge: Enhancement effects and mechanism insights

Hydroxyapatite (HAP), an inorganic mineral that is generated in situ within the core of aerobic granular sludge (AGS), plays a pivotal role in both the granulation process and system stability. Due to the slow precipitation rate of HAP, waste eggshells were utilized as a readily available source for preparing HAP by using the conventional hydrothermal (C-HAP) and microwave hydrothermal (M-HAP) methods. Subsequently, the prepared HAP was employed to promote AGS formation. Results showed that the prepared HAP exhibits superior morphology, crystallinity, functional groups, and specific surface area compared to the commercial grade HAP, whereby M-HAP outperforms C-HAP for functional groups and specific surface area. The addition of 0.5 g/L HAP facilitated the granulation process (within 20 days), leading to the development of structurally compact AGS, which was attributed to the microbial attachment onto HAP particles. Moreover, the presence of HAP greatly improved the nutrient removal efficiency (80.8 % of TN and 82.4 % of PO43−-P), most likely due to the enrichment of denitrifying and phosphorus-accumulating microorganisms (e.g., Candidatus_Competibacter and Flavobacterium). Further analysis revealed that an increase in the relative abundance of denitrification-related genes (e.g., narG and nirK) was responsible for the intrinsic mechanism behind the enhanced nitrogen removal, while phosphorus removal occurred through biomineralization and abiotic precipitation pathways. Overall, the present study highlights the potential of utilizing waste eggshells to enhance AGS systems for wastewater treatment, thus realizing a circular economy approach.

Pressure of different level PFOS on aerobic granule sludge: Insights on performance, AGS structure, community succession, and microbial interaction responses

Perfluorooctane sulfonic acid (PFOS) has received much attention due to its potential environmental risks. However, the response of aerobic granular sludge (AGS) to PFOS exposure, particularly the microbial interactions, remains unclear. In this study, we investigated the particle structure of AGS, pollutant removal performance, community succession, and microbial interaction in the AGS system under different PFOS concentrations (0.1 and 1 mg/L). The mass balance showed that PFOS was mainly removed by adsorption with a removal rate of >85 %. PFOS caused some particles to break up and decreased the average particle size from 3.37 mm to 2.64 mm. It also significantly decreased the total nitrogen and total phosphorus removal rates, which was consistent with the deterioration of microbial activity, such as denitrification rate (25 % inhibition), phosphorus uptake rate (73.19 % inhibition), and phosphorus release rate (73.33 % inhibition). PFOS promoted the secretion of extracellular polymer (EPS) in AGS, especially proteins, leading to poor particle hydrophobicity. The network analysis illustrated that PFOS slowed down the information transfer between microorganisms, and increased the competition between them, which may be responsible for the deterioration of the system performance. Connections related to rare species accounted for >75 % of the network, suggesting that rare species have an indispensable role in community information exchange. In addition, rare species acted as seed banks for microorganisms, and under PFOS stress, they transformed into keystone species, which could contribute to system stabilization. This study provides new insights into the effects of PFOS on microbial interactions in AGS systems and the roles of rare species in the AGS microbial community.