Aerobic Granular Sludge Sequencing Batch Research Articles

Aerobic granular sludge sequencing batch reactor for high strength domestic wastewater treatment: Assessing kinetic models and microbial community dynamics

The study aims to treat high strength domestic wastewater (HSDW) and cultivate microbial granular sludge in a sequencing batch reactor (SBR). A bench-scale bioreactor was used for startup. Response surface methodology (RSM) was used to determine the ideal parameters for removing organic materials from HSDW. Organic loading rate (OLR) and cycle time were the independent variables taken into consideration, while the response variable was COD removal. Results from RSM revealed that the optimal conditions for achieving > 98 % COD and TP (>80) removal were a cycle length of 6 h and an OLR of 1.5 kgCOD/m3d. The Modified-Kincannon and the Grau second-order kinetic models were compared to the COD removal rate for the SBR system. The saturation rate constant (Ni) and maximal substrate removal rate (Vc) were proposed to be 6.8 g L−1 d−1 and 0.83 g L−1 d−1, respectively, based on the modified Stover-Kincannon model. The modified Stover-Kincannon (R2 = 0.93673) and Grau second order (R2 = 0.99219) models high correlation coefficient values show that the experimental results and the prediction for the microbial aerobic granular based SBR system accord well. It was discovered that the modified Stover-Kincannon model was less suitable than the Grau second order model. The system microbial community changed because of the granulation process, as the reactor design operation had a big impact on the community structure. Decreased filamentous bacteria favoured several taxa that have been found to exist in granular biomass that is appropriate for treating wastewater. This study sheds important light on the complex interactions that occur between microbial populations and operational variables all through the granulation process in SBR.

Pharmaceutical impacts on aerobic granular sludge morphology and potential implications for abiotic removal

The goal of this study was to investigate abiotic pharmaceutical removal and abiotic pharmaceutical effects on aerobic granular sludge morphology. For 80 days, a pharmaceutical mixture containing approximately 150 μg/L each of diclofenac, erythromycin, and gemfibrozil was fed to an aerobic granular sludge sequencing batch reactor and granule characteristics were compared with those from a control reactor. Aqueous and solid phase pharmaceutical concentrations were monitored and staining was used to assess changes in biofilm structures. Solid phase pharmaceutical concentrations were elevated over the first 12 days of dosing; however, they then dropped, indicative of desorption. The lipid content in pharmaceutical-exposed granules declined by approximately half over the dosing period, though the relative concentrations of other key biofilm components (proteins, alpha-, and beta-polysaccharides) did not change. Batch experiments were conducted to try to find an explanation for the desorption observed, but reduced solid phase pharmaceutical concentrations could not be linked with the presence of common wastewater constituents such as ammonia or phosphate. Sorption of all three compounds was modeled best by the Henry isotherm, indicating that, even at 150 μg/L, granules’ sorption site coverage was incomplete. Altogether, this study demonstrates that simplified batch systems may not accurately represent the complex abiotic processes occurring in flow-through, biotic systems.

Treatment performance and microbial community structure in an aerobic granular sludge sequencing batch reactor amended with diclofenac, erythromycin, and gemfibrozil.

This study characterizes the effects of three commonly detected pharmaceuticals-diclofenac, erythromycin, and gemfibrozil-on aerobic granular sludge. Approximately 150 μg/L of each pharmaceutical was fed in the influent to a sequencing batch reactor for 80 days, and the performance of the test reactor was compared with that of a control reactor. Wastewater treatment efficacy in the test reactor dropped by approximately 30-40%, and ammonia oxidation was particularly inhibited. The relative abundance of active Rhodocyclaceae, Nitrosomonadaceae, and Nitrospiraceae families declined throughout exposure, likely explaining reductions in wastewater treatment performance. Pharmaceuticals were temporarily removed in the first 12 days of the test via both sorption and degradation; both removal processes declined sharply thereafter. This study demonstrates that aerobic granular sludge may successfully remove pharmaceuticals in the short term, but long-term tests are necessary to confirm if pharmaceutical removal is sustainable.

Assessing the impact of increasing phenol loads on the performance and stability of an aerobic granular sludge system

This work evaluated the impact of phenol on the performance and stability of an aerobic granular sludge (AGS) sequencing batch reactor (SBR) operated under alternating anaerobic-aerobic conditions for simultaneous organic matter, nitrogen, and phosphorus removal. The reactor was fed with synthetic wastewater whose phenol concentration was gradually increased from 2 to 200 mg L−1, while the other carbon source (acetate) was reduced proportionally to keep the organic loading rate invariable. Up to 20 mg L−1 of phenol, the average COD removal was above 90%, while practically full ammonium, phosphorus, and phenol removal was achieved. However, at higher phenol concentrations, the activity of polyphosphate-accumulating organisms and nitrifiers was affected. Although the overall COD removal was only slightly affected by increasing phenol loads, replacing the main carbon source from acetate to phenol changed the organic matter removal profile over the cycle, with the latter being converted mainly within the aerated stage of the SBR. As a result, there was filamentous growth that deteriorated the settling properties of the granules. The establishment of an anaerobic feast-aerobic famine regime over the non-aerated and aerated phases of the AGS SBR and the presence of a readily biodegradable substrate (such as acetate) proved to be crucial for maintaining granules stability. Having phenol as the sole organic carbon source led to complete process failure regarding nutrient removal capability.

Biodegradation of insensitive munition (IM) formulations: IMX-101 and IMX-104 using aerobic granule technology

A laboratory-scale aerobic granular sludge (AGS) sequencing batch bioreactor (SBR) was initiated in this study for the biodegradation of hazardous insensitive munition (IM) formulation constituents; 2,4-dinitroanisole (DNAN), hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX), 1-nitroguanidine (NQ), and 3-nitro-1,2,4-triazol-5-one (NTO). Efficient (bio)transformation of the influent DNAN and NTO was achieved throughout reactor operation with removal efficiencies greater than 95%. An average removal efficiency of 38.4 ± 17.5% was recorded for RDX. NQ was only slightly removed (3.96 ± 4.15%) until alkalinity was provided in the influent media, which subsequently increased the NQ removal efficiency up to an average of 65.8 ± 24.4%. Batch experiments demonstrated a competitive advantage for aerobic granular biofilms over flocculated biomass for the (bio)transformation DNAN, RDX, NTO, and NQ, as aerobic granules were capable of reductively (bio)transforming each IM compound under bulk aerobic conditions while flocculated biomass could not, thus demonstrating the contribution of inner oxygen-free zones within aerobic granules. A variety of catalytic enzymes were identified in the extracellular polymeric matrix of the AGS biomass. 16 S rDNA amplicon sequencing found Proteobacteria (27.2–81.2%) to be the most abundant phyla, with many genera associated with nutrient removal as well as genera previously described in relation to the biodegradation of explosives or related compounds.

Glycerol conversion by aerobic granular sludge

Glycerol is abundantly present in wastewater from industries such as biodiesel production facilities. Glycerol is also a potential carbon source for microbes that are involved in wastewater nutrient removal processes. The conversion of glycerol in biological phosphorus removal of aerobic granular sludge processes has not been explored to date. The current study describes glycerol utilization by aerobic granular sludge and enhanced biological phosphorus removal (EBPR). Robust granules with good phosphorus removal capabilities were formed in an aerobic granular sludge sequencing batch reactor fed with glycerol. The interaction between the fermentative conversion of glycerol and product uptake by polyphosphate accumulating organisms (PAO) was studied using stoichiometric and microbial community analysis. Metagenomic, metaproteomic and microscopic analysis identified a community dominated by Actinobacteria (Tessaracoccus and Micropruina) and a typical PAO known as Ca. Accumulibacter. Glycerol uptake facilitator (glpF) and glycerol kinase (glpK), two proteins involved in the transport of glycerol into the cellular metabolism, were only observed in the genome of the Actinobacteria. The anaerobic conversion appeared to be a combination of a substrate fermentation and product uptake-type reaction. Initially, glycerol fermentation led mainly to the production of 1,3-propanediol (1,3-PDO) which was not taken up under anaerobic conditions. Despite the aerobic conversion of 1,3-PDO stable granulation was observed. Over time, 1,3-PDO production decreased and complete anaerobic COD uptake was observed. The results demonstrate that glycerol-containing wastewater can effectively be treated by the aerobic granular sludge process and that fermentative and polyphosphate accumulating organisms can form a food chain in glycerol-based EBPR processes.

Concurrent tellurite reduction, biogenesis of elemental tellurium nanostructures and biological nutrient removal in aerobic granular sludge sequencing batch reactor

This study investigated the metabolic potential of aerobic granular sludge (AGS) to simultaneously remove tellurium oxyanion (tellurite, TeO32-), COD, ammonium-nitrogen and phosphorus in a sequencing batch reactor (SBR). AGS readily converted soluble and toxic tellurite to biogenic elemental tellurium (Bio-Te0) by following first-order reaction. Bio-Te0 was dark black in colour and largely entrapped in the granules. Although endogenous substrates acted as source of electrons for tellurite reduction, it was sustained by supplying exogenous electron donor. A lab-sale SBR was seeded with AGS and operated at varied initial tellurite concentrations for 185 days to investigate tellurite reduction and biological nutrient removal (BNR). Stable removal efficiencies of > 98% Te was achieved from simulated wastewater containing 0.01–1 mM tellurite. Soluble tellurite was converted to Bio-Te0 and retained in the AGS. SEM-EDS, XRD and Raman spectroscopy confirmed formation of Bio-Te0 and its association with the granules. Enrichment of Bacteroidia, Petrimonas sp. and several other tellurite-reducing bacteria was confirmed by 16S rRNA gene sequencing. Enrichment of ammonium oxidizing bacteria, elimination of nitrite oxidizing bacteria and profiles of reactive-nitrogen compounds suggested occurrence of nitriation-denitriation pathway. Although ammonium removal was inhibited by tellurite, recovery occurred much quickly in the presence of up to 0.1 mM tellurite. Higher P removal under tellurite-reducing condition was linked to hydroxyapatite precipitation as confirmed by EDS and P-fractionation. Efficient BNR under toxic pollutant conditions and excellent metal(loid) immobilization capabilities implicate prospective use of AGS technology in metal-laden waste management and metal(loid) biorecovery applications.

Cultivation of Nitrifying and Nitrifying-Denitrifying Aerobic Granular Sludge for Sidestream Treatment of Anaerobically Digested Sludge Centrate

In this study, three 1.2-L aerobic granular sludge sequencing batch reactors (AGS-SBRs) were used to cultivate nitrifying and nitrifying-denitrifying granules (w/supplemental carbon) and investigate sidestream treatment of synthetic-centrate and real-centrate samples from Ashbridges Bay Treatment Plant (ABTP) in Toronto, Ontario, Canada. Results showed that although the cultivation of distinct granules was not observed in the nitrifying reactors, sludge volume index (SVI30) values achieved while treating real and synthetic centrate were 72 ± 12 mL/g and 59 ± 11 mL/g (after day 14), respectively. Ammonia-nitrogen (NH3-N) removal in the nitrifying SBRs were 93 ± 19% and 94 ± 16% for real and synthetic centrate, respectively. Granules with a distinct round structure were successfully formed in the nitrifying-denitrifying SBR, resulting in an SVI30 of 52 ± 23 mL/g. NH3-N, chemical oxygen demand (COD) and phosphorus (P) removal in the nitrifying-denitrifying SBR were 92 ± 9%, 94 ± 5%, and 81 ± 14% (7th to 114th day), respectively with a low nitrite (NO2-N) and nitrate (NO3-N) concentration in the effluent indicating simultaneous nitrification-denitrification (SND) activity. High nutrient removal efficiencies via the nitrification and SND pathways shows that AGS technology is a viable process for treating sidestreams generated in a WWTP.

Distinct responses of aerobic granular sludge sequencing batch reactors to nitrogen and phosphorus deficient conditions

The nutrients availability determines efficiency of biological treatment systems, along with the structure and metabolism of microbiota. Herein nutrients deficiencies on aerobic granular sludge were comparatively evaluated, treating wastewater with mass ratios of chemical oxygen demand : nitrogen : phosphorus being 200:20:4, 200:2:4, and 200:20:0.4 (deemed as nutrient-balanced, nitrogen-deficient, and phosphorus-deficient), respectively. Results revealed that both nitrogen and phosphorus deficiencies significantly raised the effluent qualities especially nitrogen removal. However, nitrogen deficiency aroused considerable growth of filamentous bacteria, while granules kept compact structure under phosphorus deficient condition. Extracellular polymeric substances (EPS) also varied in contents and structures in response to different wastewaters. Microbial community structure analysis demonstrated that nitrogen deficiency led to lower richness and higher diversity, while the reverse was observed under phosphorus deficient condition. Nitrogen deficiency mainly induced decrease of nitrifying bacteria, while similarly phosphorus deficiency led to loss of phosphorus accumulating organisms. Dramatic enrichment Candidatus_Competibacter and filamentous Thiothrix were found under nutrients deficiencies, in which the latter explained and indicated filamentous bulking potential especially under nitrogen limited condition. Bacterial metabolism patterns verified the functions of microbial community responding to nutrients via PICRUSt2 prediction mainly by up-regulating cell motility, and cellular processes and signaling. This study could aid understanding of long-term stability of aerobic granular sludge for low-strength wastewater treatment.

Treatment of saline wastewater amended with endocrine disruptors by aerobic granular sludge: Assessing performance and microbial community dynamics

An aerobic granular sludge (AGS) sequencing batch reactor (SBR) adapted to salinity (12 g L−1 NaCl) was operated under alternating anaerobic-aerobic conditions for the treatment of synthetic saline wastewater containing endocrine-disrupting chemicals (EDCs), namely 17β–estradiol (E2), 17α–ethinylestradiol (EE2) and bisphenol-A (BPA). The SBR was intermittently fed with the EDCs at 2 mg L−1 of each compound. E2 was completely biodegraded, with 60–80% removal attained anaerobically and the remaining quickly consumed under aeration. EE2 was sorbed onto the granular sludge biomass in the anaerobic period, but it was desorbed in subsequent cycles even when the compound was not supplied to the reactor. BPA removal was poor but improved after bioaugmentation with an EDCs degrading bacteria. EDCs shock loads did not significantly affect the COD removal nor the activity of ammonium- and nitrite-oxidizing bacteria (AOB and NOB, respectively). In contrast, the activity of phosphate-accumulating organisms (PAOs) was affected, implying a decrease in P removal within the aerobic phase. AGS core microbiome grouped most bacteria belonging to the phylum Proteobacteria, followed by Bacteroidetes. The microbial profile showed that the introduction of the EDCs mixture increased the relative abundance of Chryseobacterium and Flavobacterium. AOB and NOB species were detected in the AGS biomass, with the latter showing lower relative abundance. Different PAOs, such as Rhodocyclus, Tetrasphaera and Gemmatimonas, were also part of the microbial community, but the addition of EDCs decreased significantly the relative abundance of Rhodocyclus. High microbial diversity was sustained over reactor operation, with the main bacterial groups responsible for nutrients and EDCs removal preserved in the AGS system. The results pointed to the maintenance of a core microbiome over reactor operation that may be related to the stability of the AGS process during EDCs loading.

Enhancing biological nitrogen and phosphorus removal performance in aerobic granular sludge sequencing batch reactors by activated carbon particles

Long start-up periods for aerobic granular sludge (AGS) formation and establishment of P removal pathways are challenges for widespread implementation of AGS process. External additives such as activated carbon (AC) attracted interest for accelerating AGS formation. However, the roles of AC in granulation and biological nutrient removal (BNR) are not understood. Here, the role of AC was investigated in decreasing start-up periods in AGS formation and BNR under different carbon substrate conditions (i.e., acetate (HAc), propionate (HPr) and HAc-HPr) in sequencing batch reactors (SBRs). AC addition increased aggregation index and settleability of activated sludge (AS) inoculum which minimized AS washout from SBRs. AC addition hastened AGS formation and establishment of BNR pathways by facilitating AS retention and biofilm formation. Feeding HAc or HAc-HPr supported better granulation (MLSS: 6–7 g l−1, SVI: 30–40 ml g−1) than HPr (MLSS: 4 g l−1, SVI: 70). The start-up periods for efficient total nitrogen (TN) removals were decreased to 22 and 16 d from 38 to 25 d, respectively, in AC augmented SBRs fed with either HAc or HAc-HPr. TN removals were higher at ≥95% in HAc or HAc-HPr fed SBRs. Total phosphorus (TP) removals were also higher in AC-augmented SBRs at 80% and ≥90% in HAc and HAc-HPr fed SBRs, respectively. In contrast, TN and TP removals were lower at 70% and 35%, respectively, in HPr fed SBR. Ammonium was primarily removed via nitritation-denitritation pathway. Phosphorus removal was at 1.7 to 2-fold higher in AC augmented SBRs and driven by enhanced biological phosphorus removal (EBPR) pathway. MiSeq sequencing and qPCR revealed higher enrichment of polyphosphate accumulating organisms (PAOs), denitrifying PAOs, and ammonia oxidizers in AC-augmented SBRs fed with HAc or HAc-HPr. This study demonstrates that AC addition can be considered for enrichment of PAOs and establishment of EBPR in aerobic granular SBRs.

COD, nitrogen and phosphorus removal from simulated sewage in an aerobic granular sludge in the absence and presence of natural and synthetic estrogens: Performance and biomass physical properties assessment

In this research, the effect of two endocrine-disrupting chemicals (EDCs), namely 17β-estradiol (E2) and 17α-ethinylestradiol (EE2), on the simultaneous removal of organic matter, nitrogen and phosphate in an aerobic granular sludge (AGS) sequencing batch reactor (SBR) was addressed. The reactor operation was divided into Stage I (Control) and Stage II, with E2 and EE2 (20 µg L−1) added to the reactor influent only in the latter. The results showed that ammonium removal substantially dropped from 97% to 58% when the reactor was fed with EDCs. However, gradual recovery of nitrifying activity was observed, and complete ammonium oxidation was re-established. Phosphate removal also became unstable in the presence of EDCs, dropping from 100% to 67%. Nevertheless, the estrogens seemed to have only an initial adverse effect on polyphosphate accumulating bacteria, and P removal capacity was recovered. Regarding the granular biomass physical properties, a decrease in the granules average density was observed at the beginning of Stage II, a result associated with a slight disintegration of the granules surface, affecting their stability. Under these conditions, an increase in total proteins and polysaccharides within the biomass was also noticed. Nevertheless, the density of the granules quickly returned to the original values. These findings suggest an adaptation of the microorganisms harboured in the AGS system to the presence of EDCs.

Comparative performance of activated sludge and aerobic granular sludge sequencing batch reactors for removing metalloid SeIV/VI oxyanions

Biotransformation of soluble selenite (SeIV) and selenate (SeVI) to biomass-associated Se0 (Bio-Se) provides a solution for mitigating Se pollution coupled to securing scarce element. Here, we demonstrated an efficient Se oxyanion removal process by converting to Bio-Se using aerobic granular sludge (AGS). SeIV and SeVI removal was dependent on lactate availability, and proportional to initial Se and biomass concentrations. Laboratory-scale sequencing batch reactors (SBR) were operated for 100 days by feeding an influent containing 100 μM SeIV, 100 μM SeVI, or 100 μM each of SeIV and SeVI to evaluate Se and nutrient removal performance. At 34 h hydraulic retention time, complete and sustained removal of influent SeIV, SeVI or SeIV-SeVI was achieved in 24 h cycles containing anaerobic (8 h) and aeration (15 h) phases. Removal of NH4+-N was sustained in AGS reactor through nitritation-denitritation under Se-loading conditions. AGS process demonstrated SeIV/VI, COD, ammonia, and total nitrogen removal efficiencies of 99 %, 99 %, 99 % and 98 %, respectively. Both batch and SBR experiments revealed that AGS is superior to activated sludge in achieving simultaneous SeIV/VI oxyanion and nutrient removal. This study shows that AGS process could be applied for treating acid mine and agricultural drainage that contain Se oxyanions and ammoniacal-nitrogen.

Biological removal processes in aerobic granular sludge exposed to diclofenac

ABSTRACT Diclofenac is a worldwide consumed drug included in the watch list of substances to be monitored according to the European Union Water Framework Directive (Directive 2013/39/EU). Aerobic granular sludge sequencing batch reactors (AGS-SBR) are increasingly used for wastewater treatment but there is scant information on the fate and effect of micropollutants to nutrient removal processes. An AGS-SBR fed with synthetic wastewater containing diclofenac was bioaugmented with a diclofenac degrading bacterial strain and performance and microbial community dynamics was analysed. Chemical oxygen demand, phosphate and ammonia removal were not affected by the micropollutant at 0.03 mM (9.54 mg L–1). The AGS was able to retain the degrading strain, which was detected in the sludge throughout after augmentation. Nevertheless, besides some adsorption to the biomass, diclofenac was not degraded by the augmented sludge given the short operating cycles and even if batch degradation assays confirmed that the bioaugmented AGS was able to biodegrade the compound. The exposure to the pharmaceutical affected the microbial community of the sludge, separating the two first phases of reactor operation (acclimatization and granulation) from subsequent phases. The AGS was able to keep the bioaugmented strain and to maintain the main functions of nutrient removal even through the long exposure to the pharmaceutical, but combined strategies are needed to reduce the spread of micropollutants in the environment.

Biodegradation of natural and synthetic endocrine-disrupting chemicals by aerobic granular sludge reactor: Evaluating estrogenic activity and estrogens fate.

In this study, the biodegradation of endocrine-disrupting chemicals (EDCs) (namely the natural and synthetic estrogens 17β-estradiol (E2) and 17α-ethinylestradiol (EE2), respectively) was assessed in an aerobic granular sludge (AGS) sequencing batch reactor (SBR) treating simulated domestic sewage. To better understand the fate of these compounds, their concentrations were determined in both liquid and solid (biomass) samples. Throughout the operation of the reactor, subjected to alternating anaerobic and aerated conditions, the removal of the hormones, both present in the influent at a concentration of 20μgL-1, amounted to 99% (for E2) and 93% (for EE2), with the latter showing higher resistance to biodegradation. Through yeast estrogen screen assays, an average moderate residual estrogenic activity (0.09μgL-1 EQ-E2) was found in the samples analysed. E2 and EE2 profiles over the SBR cycle suggest a rapid initial adsorption of these compounds on the granular biomass occurring anaerobically, followed by biodegradation under aeration. A possible sequence of steps for the removal of the micropollutants, including the key microbial players, was proposed. Besides the good capability of the AGS on EDCs removal, the results revealed high removal efficiencies (>90%) of COD, ammonium and phosphate. Most of the incoming organics (>80%) were consumed under anaerobic conditions, when phosphate was released (75.2 mgP L-1). Nitrification and phosphate uptake took place along the aeration phase, with effluent ammonium and phosphate levels around 2mgL-1. Although nitrite accumulation took place over the cycle, nitrate consisted of the main oxidized nitrogen form in the effluent. The specific ammonium and phosphate uptake rates attained in the SBR were found to be 3.3 mgNH4+-N gVSS-1.h-1 and 6.7 mgPO43--P gVSS-1 h-1, respectively, while the specific denitrification rate corresponded to 1.0 mgNOx--N gVSS-1 h-1.

Microbial analysis for the ammonium removal from landfill leachate in an aerobic granular sludge sequencing batch reactor

In this study, a laboratory-scale sequencing batch reactor (SBR) equipped with aerobic granular sludge (AGS) technology was continuously operated for 220 days to remove ammonium from an existing landfill leachate. The ammonium removal was characterized by polymerase chain reaction (PCR)-denaturing gradient gel electrophoresis (DGGE) technology. This method helped to analyze the long-term community structural stability of ammonia-oxidizing bacteria (AOB), nitrite-oxidizing bacteria (NOB) and denitrifying bacteria (DB) throughout the experiment. Simultaneously, 16S rRNA gene cloning and sequencing analysis identified the dominant species of different microbial species. Experimental results confirmed that ammonium removal was inhibited at the high nitrogen loading rate (NLR) stage while the low NLR stage achieved satisfactory ammonium removal. Moreover, the findings demonstrated that functionally stable wastewater treatment bioreactors facilitated the occurrence of stable microbial community structures.

Impact of hydraulic retention time on swine wastewater treatment by aerobic granular sludge sequencing batch reactor.

In this study, the effect of hydraulic retention time (HRT) on the performance of aerobic granular sludge (AGS) treating swine wastewater (SW) in sequencing batch reactor (SBR) was investigated. The HRT was set at 4.8, 6, 8, 12, and 16 h and performed in five parallel aerobic granular sludge sequence batch reactors (AGSBRs), respectively. The results showed that sedimentation performance and biomass concentration were improved by decreasing the HRT from 16 to 8 h. However, when further decreasing HRT from 8 to 4.8 h, the AGS performance became worse. The AGS process with HRT of 8 h exhibited high pollutant removal efficiency, and an abundant microbial community and a stable microbial community structure were observed. High-throughput 16S rRNA analysis revealed that there were significant differences between the microorganisms in AGS samples with HRT of 8 h at the phylum level and that the dominant microbes changed as the process proceeded. At the genus level, the species and relative abundance of microorganisms gradually evolved for AGS stability in all cases.

Effect of carbon source on pollutant removal and microbial community dynamics in treatment of swine wastewater containing antibiotics by aerobic granular sludge

Aerobic granular sludge sequencing batch reactor (AGSBR) is a promising approach for wastewater treatment. In the paper, the effects of methanol, starch and sucrose as carbon sources on the treatment of swine wastewater (SW) containing antibiotics by aerobic granular sludge (AGS) were studied. The results revealed that the carbon sources could affect the morphology, biomass, and settleability of AGS, and AGS could maintain a better sludge performance when sucrose was used as carbon source. The pollutants (ammonium nitrogen (NH+ 4-N), organic matter and total phosphorus (TP)) in SW also had a good removal effect, and the removal rates reached 81.14%, 96.83% and 97.37% respectively. The removal efficiencies of tetracycline (TC) and oxytetracycline (OTC) from SW were the best when sucrose as co-metabolic matrix by microorganisms. The analysis of miseq pyrosequencing demonstrated that carbon sources with methanol, starch and sucrose improved the diversity of microbial community in AGS, and the dominant bacteria also changed. The dominant groups involved in TC and OTC, removal at different classification levels suggested that the formation of bacterial communities was determined by carbon sources.

Elevated salinity deteriorated enhanced biological phosphorus removal in an aerobic granular sludge sequencing batch reactor performing simultaneous nitrification, denitrification and phosphorus removal

Hypersaline wastewater may pose threats to biological wastewater treatment processes. An aerobic granular sludge-based sequencing batch reactor (SBR) performing simultaneous nitrification, denitrification and phosphorus removal (SNDPR) was evaluated with increased salinity from 1 to 2 % (w/v). Nitrogen removal performance was unaffected by salinity up to 20 g/L in terms of reliable and efficient nitrification and denitrification. Enhanced biological phosphorus removal (EBPR) process was completely deteriorated at salinity up to 2 %, in contrast to excellent phosphorus removal at 1 %. Profiles of phosphorus over one cycle demonstrated that higher salinity not only inhibited anaerobic phosphorus release but also impeded aerobic/anoxic phosphorus uptake. Illumina MiSeq sequencing revealed multiple halophilic and non-halophilic bacteria within aerobic granules with family Anaerolineaceae being the predominant potential salt adapter. Besides, ammonia oxidizing bacteria (AOB), glycogen accumulating organisms (GAOs) were more tolerant to salt than nitrite oxidizing bacteria (NOB) and phosphorus accumulating organisms (PAOs) and denitrifying PAOs (DNPAOs). These results deciphered the resilience of aerobic granular sludge-based biological nitrogen and phosphorus removal processes to hypersaline stress.

Robustness of an aerobic granular sludge sequencing batch reactor for low strength and salinity wastewater treatment at ambient to winter temperatures

Acclimation performances and characteristics of aerobic granular sludge to salt and temperature were investigated in a sequencing batch reactor (SBR) performing simultaneous nitrification, denitrification and phosphorus removal (SNDPR). The aerobic granular SNDPR system was firstly subjected to low salinity (0.5%, w/v) at moderate temperature (> 15 ℃) and subsequent low temperature (< 15 ℃). The shock loading of salinity temporarily deteriorated biological phosphorus removal, while dual stresses of salinity and low temperature induced temporary inhibition on both nitrogen and phosphorus removal. Both salinity and low temperature stimulated the settleability of aerobic granules, accompanied with decreased ratios of protein to polysaccharide (PN/PS). Illumina MiSeq sequencing revealed that salinity rarely affected bacterial richness, but significantly decreased the diversity. Whereas low temperature strengthened both bacterial richness and diversity. Phyla Proteobacteria, Chloroflexi and their sub-groups acted as the main halophilic bacteria while Proteobacteria was also psychrophilic. The functional bacteria such as nitrifiers, denitrifiers, and phosphorus removal bacteria exhibited greater tolerance to salt and low temperature than glycogen accumulating organisms (GAOs). Overall, the present study demonstrated the resilience and robustness of aerobic granular sludge toward salinity and low temperature, which could aid the knowledge of saline wastewater treatment by aerobic granular sludge.