Aedes Sp Mosquitoes Research Articles

Identifikasi Nyamuk Aedes aegypti dan Aedes albopictus Pada Kejadian Luar Biasa Infeksi Chikungunya Di Bali

Chikungunya is a re-emerging disease that occurs due to the bites of Aedes sp-infected Chikungunya virus (CHIKV). Chikungunya infection is a serious issue with high fever for 3 - 5 days as the indication and until now there is no Chikungunya vaccine. This is a descriptive study with a cross-sectional approach. The research samples were mosquitoes captured on 2 sampling points conducted in Peregae hamlet, Mengwi, and Buana Desa village, Padangsambian sub-district in December 2021. The aim of this study was to identify the Aedes aegypti and Aedes albopictus mosquitoes as vectors of CHIKV infection in areas with an increasing number of CHIKV outbreak cases in Bali. The sampling technique was performed using total sampling utilizing a light trap installed in the room from the afternoon until the morning. The total number of mosquitoes captured in the two locations was 101, consisting of 46 non-Aedes sp (45.5%) and, 55 Aedes sp (53.9%). Captured Aedes sp mosquitoes consisted of 2 species, that is 6 Ae. albopictus (5.9%) and 49 Ae. aegypti (48.5%). Ae. aegypti mosquitoes were more commonly found as a vector for CHIKV infection in Peregae hamlet, Mengwi village, and Buana Desa hamlet, Padangsambian sub-district

Dengue Virus Detection Using Rt-Pcr Method In Ternate City, North Maluku, Indonesia

The Dengue disease is caused by a virus from the family Flaviviridae and there are four distinct, but closely related, virus serotypes that cause dengue (DENV-1, DENV-2, DENV-3 and DENV-4). Dengue virus detection is very important to determine the virus serotype that develops in an area. There are several methods to detect dengue virus based on different targets, namely nucleic acids, viral antigens, and antibodies. This study aims to determine the type of dengue virus serotype in four working areas of the Public Health Center in Ternate City. The sample in this study was the eggs of Aedes sp mosquitoes caught in the homes of DHF sufferers and the houses around them. Collection of mosquito eggs Ae. Aegypti was carried out in four Puskesmas working areas in Ternate City. Ovitrap installation was carried out in 200 houses, with a total of 400 ovitraps. Rearing eggs and dengue virus detection were carried out at the Microbiology Laboratory, LITBAGKES Banjarnegara. The method used in this research is the RT-PCR test. The data analysis of the research results was carried out descriptively. Based on the identification results, the mosquito used in this study was the Aede aegypti mosquito. The results of electrophoresis produced viral RNA with a base length of 100 bp, while the target RNA that had been determined were Den1 = 342 bp, Den2 = 251 bp, Den3 = 538 bp. Den4 = 752 bp. The results of the RT-PCR examination showed that the Ae. aegypti in the four working areas of the Puskesmas did not contain the dengue virus (virus negative).

Status Resistensi dan Keberadaan Virus Dengue pada Nyamuk Aedes Sp di Kelurahan Pinang Kencana, Kota Tanjungpinang, Kepulauan Riau

Tanjungpinang Timur Village is an endemic area, and every year there is a death rate due to dengue fever. This study aimed to map the resistance and presence of the dengue virus in Aedes sp mosquitoes in Pinang Kencana Village. This research was observational with a descriptive approach. The samples were mosquitoes bred in the Vector Laboratory of Poltekkes, Ministry of Health, Tanjungpinang, from eggs trapped in ovitraps distributed in the study area. A total of 78 ovitraps were placed inside the house, 45 were positive, while 45 positive ovitraps were placed outside the house out of a total of 84 ovitraps. Ovitrap index numbers outside (57.7%) and inside the house (53.6%) with an overall ovitrap index of 55.6%. The resistance test results using the CDC bottle bioassay method showed that Aedes sp mosquitoes from Pinang Kencana Village were resistant to the pesticide deltamethrin dose of 10 g/bottle with a diagnosis time of 30 minutes with a mosquito mortality percentage of 68.33%. The PCR results for dengue virus in the sample mosquitoes showed no presence of dengue virus in the mosquito samples, both DEN-1, DEN-2, DEN-3, and DEN-4 serotypes.

Pelatihan dan Pendampingan Jumantik Dalam Upaya Pencegahan DBD pada Siswa SMPN 12 Kota Tanjungpinang

Pinang Kencana Village is an area that is prone to dengue fever with a population of 47,509 people. In 2017 the Pinang Kencana Village occupied the highest dengue cases as many as 52 people. Every year, Pinang Kencana Village is an area that has the highest dengue cases. This is partly influenced by the behavior that often accommodates clean water during the rainy season and in the summer and is also added to the clean water reservoir in the Pinang Kencana sub-district school that does not have a lid so that it can cause the development of Aedes Sp mosquitoes. DHF transmission does not only occur in the neighborhood, but also in schools. Schools are one of the potential places that can be a breeding ground for Aedes mosquitoes, so they can become places for dengue transmission. Places that can be a breeding ground for mosquitoes in schools include water reservoirs, school toilets, flower pots, etc. And the school is also a place where a group of people gather on a regular basis which takes place almost every day, so that it becomes one of the priority places for periodic monitoring of the presence of larvae so that they do not become a source of dengue transmission.

UJI EFEKTIVITAS EKSTRAK BUAH BALONGGA (Cucumis melo L. forma agrestis) SEBAGAI INSEKTISIDA ALAMI TERHADAP NYAMUK Aedes sp.

Background: Aedes sp is a vector of Dengue Hemorrhagic Fever (DHF). DHF is a disease caused by the dengue virus of the genus Flavivirus, family Flaviviridae. DHF is transmitted from person to person through the bite of the Aedes aegypti and Aedes albopictus (female) mosquitoes. Balongga fruit is a wild plant typical of Southeast Sulawesi which is usually consumed by the people of Southeast Sulawesi as a food ingredient such as vegetables and to add flavor to fish dishes. Balongga fruit contains secondary metabolites, namely flavonoids, saponins and tannins which have the ability to inhibit the growth of larvae and mosquitoes.Objective: The purpose of this study was to determine the results of the effectiveness test of the extract of Balongga fruit (Cucumis melo L. forma agrestis) as a natural insecticide against Aedes sp.Methods: This research was conducted experimentally, in the test using four treatments with variations in concentration, namely 20%, 40%. 60% and 80%, negative control (aquadest) and positive control X-Electric (transflutrin 12.38 g/l)), and the number of mosquitoes used was 25 in each treatment with three repetitions. The observation time required in this study is 1440 minutes. Observations were also made by counting the dead mosquitoes in each treatment with time intervals of 5, 10, 20, 40, 60, 120, 240, 480, and 1440 minutes. The data in this study were analyzed using the one-way ANOVA test.Conclusion: The results showed that the 80% concentration of Balonga fruit flesh extract was the most effective as a natural insecticide to kill Aedes sp mosquitoes with an interval of 240 minutes.

Housewives Behavior in Effort to Overcome Dengue Fever

Dengue Hemorrhagic Fever is still a health problem. NTT is one of the provinces with the third highest number of DHF cases in Indonesia in 2019. Oesapa Village is the highest DHF distribution area in Kupang City, where there were 52 DHF cases in early 2019. The transmission of DHF cannot be separated from the presence of Aedes sp mosquitoes as vectors. The initial survey showed that all household in Oesapa sub-district still had a standard free larvae rate (<95%). The Ministry of Health launched the One Home One Jumantik movement to support the PSN 4M Plus acculturation program, which required the active role of the family, especially a housewife as an independent larva monitor. The general objective of this research is to find out the behavior of housewives as larva monitors at household. The research design used was Cross Sectional Study. Data were analyzed descriptively and presented in the form of narration, tables and figures. The results showed that housewives' knowledge in the prevention and control of dengue vector was classified as good category. Most housewives have a positive attitude in the prevention and control of dengue vector. Also the behavior of housewives in the efforts to prevent and control DHF vectors have been classified as good categories. The community is expected to continue to actively participate and continue to make efforts to control the vector of DHF by involving housewives as agents of change.

Pemetaan Resistensi Nyamuk Aedes sp Terhadap Malation dan Kepadatan Vektor Demam Berdarah Dengue (DBD) di Sulawesi Utara

The event of mosquito immunity or resistance to malation is one of the main inhibitors of chemical eradication of vectors with insecticides. Vector resistance to insecticides is a global phenomenon, especially for managers of vector-borne control programs in Indonesia. The aims of this study was to map the resistance of Aedes sp mosquitoes to the malation and vector density of Dengue Hemorrhagic Fever (Aedes sp) in North Sulawesi Province in 2019. This research was descriptive in order to obtain a description of pre-adult vector density and to map the status of Dengue Hemorrhagic Fever vector resistance (Aedes sp) against malation. Resistance status data using data from 2015-2018 research. The population in this study are houses in North Minahasa Regency, Minahasa Regency, Tomohon City, Bitung City and Manado City. The sample for vector density is a larva survey of 100 resident houses which includes case houses and houses around DHF cases. North Minahasa Regency (Wautumou Village), Minahasa Regency (Papakelan Village), Tomohon City (Rurukan Village), Bitung City (Girian Permai Village), Manado City (Malalayang Village 2). Determination of the sample based on the highest case in each district in the city during the DHF vector resistance test in 2015-2018. The results showed that the average density of HI vectors (31.%), BI (17.5) and CI (34.8%) with resistance status in the four districts of the mosquito vector city of DHF were resistant and one district (North Minahasa) with a vulnerable status. Resistance and density vectors are digitally mapped.

POPULASI AEDES SP YANG DOMINAN SEBAGAI VEKTOR POTENSIAL DENGUE DI KOTA JAMBI

Ringkasan
 Kota Jambi masih menjadi kontributor penderita DBD tertinggi dari 11 Kabupaten/Kota yang ada di Propinsi Jambi. Sasaran pengendalian terhadap nyamuk Aedes sp belum selektif dilakukan karena belum ada informasi terkait keragaman populasi serta karakteristik perindukan Aedes sp pada daerah endemis DBD di Kota Jambi.
 Permasalahan yang diteliti tentang vektor potensial dengue yang lebih dominan, dan jenis perindukannya. Sehingga tujuan dari penelitian ini adalah diketahuinya populasi dominan antara Ae. aegypti dan Ae. albopictus yang tertangkap dengan Backpack Aspirator serta karakteristik perindukannya.
 Metode penelitian adalah Deskriptif dengan pendekatan studi eksplorasi nyamuk Aedes sp di 5 kelurahan tertinggi DBD, masing-masing kelurahan sebanyak 100 rumah yang dipilih secara random sampling.
 Hasil penelitian memberikan informasi bahwa Aedes albopictus lebih dominan ditemukan, dengan rasio nyamuk Aedes albopictus tertinggi pada Kelurahan Lingkar Selatan yaitu (11 : 1), kemudian Kelurahan Kenali Besar (9 : 1). Begitu juga dengan rasio larva Aedes albopictus, terdapat 3 kelurahan yang berada di pinggiran kota (Kenali Bessar, Talang Bakung dan Lingkar Selatan) memiliki rasio larva Aedes albopictus 6 kali lebih besar di bandingkan kelurahan yang tidak berada dipinggiran kota. Penelitian ini juga menemukan Bak mandi (47%) dan Drum (28,5%) sebagai tempat perindukan Aedes sp terbanyak.
 Perlu dilakukan penelitan lanjutan mengenai persentase Aedes sp yang terinfeksi virus dengue, sehingga dapat membuktikan bahwa Aedes albopictus sebagai vektor utama DBD di Kota Jambi

Pola Kepadatan Populasi Vektor Penyakit DBD Di Permukiman Penduduk Bantaran Sungai Martapura Kecamatan Martapura Timur Tahun 2017

Martapura river water is one of the source of water used for daily use by residents who live along the banks of the river Martapura. Almost every house along the river Martapura does not have adequate clean water facilities and is highly dependent on river water. The objective of the study was to know the pattern of population density of dengue fever vector in residential area of ​​river Martapura. This type of analytic research, using a cross sectional design. The population of all Aedes sp mosquitoes and all residents in the residential area of ​​river Martapura. The sample is partially Aedes sp mosquitoes and residents in the residential area of ​​the river Martapura river, the village Antasan Senor, Antasan Senor Ilir, and the village of Mekar). Purposive Sampling Sampling Technique. Methods of gathering observations, interviews and identification. Analytical data processing and analysis with one way ANOVA test. The results showed that the population density vector category "High" DF = 4.7 and ABJ 71.7%. The result of statistical test of population vector density pattern there is no significant difference (p-value> 0,05). The environmental condition of the settlement is quite clean, breading place of the existence of larvae is not found, the number of TPA varies in the form of plastic, rubber, cement, iron / aluminum. Type / material of positive landfill larvae made of plastic material. Suggestion of research, for related institution to socialize and mobilize community in PSN activity of 3M + movement routinely, larva survey once a week, train Jumantik officer, public health education activity and integrated vector eradication (physical, chemical or biological) and community PHBS found the family / community experiencing symptoms of dengue disease immediately report / bring to the nearest health officer / facility.

Relationship of Rainfall, Population Density, and Human Behavior with DHF Incidence in Makassar City

Dengue Hemorrhagic Fever (DHF) is an infectious disease caused by the dengue virus which can be transmitted through the bites of Aedes sp mosquitoes and can endanger the surrounding environment. This study aims to determine the relationship of rainfall, population density, and human behavior which were the usage of mosquito net and repellent with DHF incidence. This study was conducted in Paccerakang village of Makassar city. The type of this study was observational analytic with a cross-sectional study design. Sample used was 100 houses which obtained by using purposive sampling method. The results showed that there was no relationship between rainfall (p = 0.638> 0.05), population density (p = 0.685>0.05), and human behavior which were using mosquito net (p = 0, 481> 0, 05) and repellent (p = 0, 481> 0, 05) with population density. The community is expected to be more proactive in seeking information on prevention of dengue and the health care should provide information on the importance of maintaining environmental cleanliness.

Effectiveness of Household Insecticides to Reduce Aedes Aegypti Mosquitoes Infestation: A Community Survey in Yogyakarta, Indonesia

Dengue prevention and controleffortslargerlyrely on insecticide application for vector control. Majority people used household insecticide to reduce mosquito infestation. However, information about effectiveness of household insecticide to reduce mosquitoes infestation is still limited. Aims of this research is to analyse the effectiveness of household insecticide use to reduce the Aedes sp infestation in Yogyakarta, Indonesia. A questionnaire was administered to 900 respondents in three regencies in Yogyakarta (Sleman, Bantul and Gunung Kidul), and larvae collection was carried out in the house of respondents. The results showed that larvae indices in three regencies in Yogyakarta Province was >30%, indicating this area categorized in high mosquito populations. Majority of household insecticide in Sleman and Bantul was electric, 34.75 and 39.6% respectively, while mosquito coils was dominants in Gunung Kidul (51.6%). Based on chi-square test, the use of household insecticide had no significant effect on the infestations of Aedes sp mosquitoes. This facts should be a supported evidence that it is crucial to maintain the eradication of mosquito breeding site program in community, since the use of household insecticide is ineffective to reduce the mosquito infestations.

KEMELIMPAHAN DAN AKTIVITAS MENGGIGIT NYAMUK Aedes sp PADA DAERAH ENDEMIS DEMAM BERDARAH DENGUE DI KOTA METRO, LAMPUNG

Aedes sp mosquitoes as vectors of disease Dengue fever remains a serious health problem in Indonesia. This research is important to be studied because by knowing the abundance and activity of the mosquito bite would help in early warning and the work were done as a form of prevention / preventive direct contact with mosquitoes. The research was conducted in the Village Metro District of Metro Center on RT 09, RT 11 and RT 10. RT 12. Catching adult mosquitoes do 1 time per week in the month of April 2014 from the hours 06: 00-18: 00. The method used for catching mosquitoes is to use bait human foot (human landing) for 20 minutes. Results showed the peak activity occurred 2 times, ie at 09:00 to 9:55 and at 16:00 to 16:55 pm. Blood biting behavior of Aedes sp in Metro City is anthropophilic. Values for the relative abundance of fishing activity in the house (endofagik) of 7.28, while for fishing activities outside the home (eksofagik) 9.5.Kata Kunci: Aedes sp, anthropofilik, human baiting.

RELATIONSHIP BETWEEN KNOWLEDGE, ACTION OF FAMILY IN DCB (DRAIN, CLOSE, AND BURY) PROGRAM, AND THE EXISTENCE OF EGGS OF AEDES AEGYPTI MOSQUITO ON OVITRAP IN KANDAI KENDARI, INDONESIA

Background: Dengue Hemorrhagic Fever (DHF) is a contagious disease caused by the dengue virus infection through Aedes aegypti mosquito. DHF often causes epidemics and Extraordinary Events (EE) in tropical and subtropical regions, including Indonesia.Objective: This study aims to examine the relationship between knowledge and society action on the prevention of dengue with the amount of mosquitoes trapped in ovitrap in Kandai, Kendari, Indonesia.Methods: The research method was an observational study with cross sectional design. The research was conducted at Kandai village, Kendari on May to July 2014. It was 87 homes with 87 families were recruited as sample size for this study. Ovitrap was used inside and outside of their homes.Results: DCB family knowledge and the existence of Aedes sp mosquitoes eggs on ovitrap were analyzed and observed in this study. Chi square test showed that X2 count value (1.261) was less than X2 table value (3.814) at error level 5% (α = 0.05) indicated that Ha was rejected and Ho was accepted. Meanwhile, Chi square test for the action of the family about DCB and the existence of eggs Aedes sp mosquito on ovitrap showed that X2 count value (4.115) was greater than X2 table value (3.814) at the error level 5% (α = 0.05), indicated that Ho was rejected and Ha was accepted.Conclusions: It was concluded that (1) there was no relationship between DCB family knowledge and the existence of Aedes sp mosquitoes eggs on ovitrap, and (2) there was a relationship between the action of family about DCB and the existence of aedes sp mosquitoes eggs on ovitrap in Kandai Kendari.

Rapid detection of European orthobunyaviruses by reverse transcription loop-mediated isothermal amplification assays

The development of reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) assays are described herein for the detection of two orthobunyaviruses (Bunyaviridae), which represent the two main serogroups found in mosquitoes in Central Europe. The RT-LAMP assays were optimized for the detection of Ťahyňa virus (a California encephalitis group virus found in Aedes sp or Ochlerotatus sp mosquitoes) and Batai virus (also called Čalovo virus, a Bunyamwera group virus found in Anopheles maculipennis s.l. mosquitoes) nucleic acid using endemic European virus isolates. The sensitivity of the RT-LAMP assays was determined to be comparable to that of conventional tests, with a limit of detection<0.1 pfu per reaction. The assays can be performed in 60min under isothermal conditions using very simple equipment. Furthermore, it was possible to proceed with the assays without nucleic acid extraction, albeit at a 100-fold loss of sensitivity. The RT-LAMP assays are a sensitive, cost-efficient method for both arbovirus surveillance as well as diagnostic laboratories to detect the presence of these endemic orthobunyaviruses.

Detecção de Aedes aegypti e Aedes albopictus, na zona urbana do município de Catanduva-SP, após controle de epidemia de dengue

After the realization of control research that had in view the transmission of dengue virus, we started to monitor two kinds of entomological vigilance, Breteau Index and ovitrap. We intended to evaluate the necessary time elapsed before Aedes sp mosquitoes were again detected at the urban area of Catanduva s town (SP). The ovitraps showed positiveness for the Aedes aegypti two months after the control research, while the Breteau Index became positive only at the fourth month after the end of the referred research.