Advanced Nanomaterials Research Articles

Analysis of Giant-Shell CdSe/CdS Quantum Dots via Analytical Ultracentrifugation Combined with Spectrally Resolved Photoluminescence.

Knowledge of the structure-property relationships of functional nanomaterials, including, for example, their size- and composition-dependent photoluminescence (PL) and particle-to-particle variations, is crucial for their design and reproducibility. Herein, the Angstrom-resolution capability of an analytical ultracentrifuge combined with an in-line multiwavelength emission detection system (MWE-AUC) for measuring the sedimentation coefficient-resolved spectrally corrected PL spectra of dispersed nanoparticles is demonstrated. The capabilities of this technique are shown for giant-shell CdSe/CdS quantum dots (g-QDs) with a PL quantum yield (PL QY) close to unity capped with oleic acid and oleylamine ligands. The MWE-AUC PL measurements are calibrated and validated with certified fluorescence standards. The spectrally corrected and size-dependent PL spectra of the g-QDs derived from a single MWE-AUC experiment are then analyzed and compared with the results of single-particle spectroscopic studies, yielding the PL spectra, decay kinetics, and blinking behavior of individual g-QDs. This study underlines the vast potential of MWE-AUC with in-line optical detection for the characterization of advanced nanomaterials with a complex structure.

Revealing the diverse electrochemistry of nanoparticles with scanning electrochemical cell microscopy.

The next generation of electroactive materials will depend on advanced nanomaterials, such as nanoparticles (NPs), for improved function and reduced cost. As such, the development of structure-function relationships for these NPs has become a prime focus for researchers from many fields, including materials science, catalysis, energy storage, photovoltaics, environmental/biomedical sensing, etc. The technique of scanning electrochemical cell microscopy (SECCM) has naturally positioned itself as a premier experimental methodology for the investigation of electroactive NPs, due to its unique capability to encapsulate individual, spatially distinct entities, and to apply a potential to (and measure the resulting current of) single-NPs. Over the course of conducting these single-NP investigations, a number of unexpected (i.e. rarely-reported) results have been collected, including fluctuating current responses, and carrying of the NP by the SECCM probe, hypothesised to be due to insufficient NP-surface interaction. Additionally, locations with measurable electrochemical activity have been found to contain no associated NP, and conversely locations with no activity have been found to contain NPs. Through presenting and discussing these findings, this article seeks to highlight complications in single-NP SECCM experiments, particularly those arising from issues with sample preparation.

Emerging devices based on chiral nanomaterials.

As advanced materials, chiral nanomaterials have recently gained vast attention due to their special geometry-based physical and chemical properties. The fast development of the related science and technology means that various devices involving polarization-based information encryption, photoelectronic and spintronic devices, 3D displays, biomedical sensors and measurement, photonic engineering, electronic engineering, solar devices, etc., been explored extensively. These fields are at their beginning, and much effort needs to be made, including improving the optical, electronic, and magnetic properties of advanced chiral nanomaterials, precisely designing materials, and developing more efficient construction methods. This review tries to offer a whole picture of these state-of-the-art conditions in these fields and offers perspectives on future development.

In situ transmission electron microscopy insights into nanoscale deformation mechanisms of body-centered cubic metals.

Nanostructured body-centered cubic (BCC) metals exhibit remarkable mechanical properties under various stress fields, making them promising candidates for novel micro/nanoelectromechanical systems (M/NEMS). A deep understanding of their mechanical behaviors, particularly at the atomic scale, is essential for optimizing their properties and expanding their applications at the nanoscale. Newly developed nanomechanical testing techniques within transmission electron microscopy (TEM) provide powerful tools for uncovering the atomic-scale microstructural evolution of nanostructured BCC materials under external forces. This article reviews recent progress in the experimental methods used in in situ TEM nanomechanical testing and the achievements of these techniques in understanding the deformation mechanisms of BCC nanomaterials. By outlining the current challenges and future research directions, this review aims to inspire continued exploration in the nanomechanics of BCC metals, contributing to the development of advanced BCC nanomaterials with tailored mechanical properties.

Application of Carrera Unified Formulation and Hybrid AI-Composition with CNN and ReliefF feature selection: presenting some useful suggestions for improving the stability of football and sport equipment via advanced nanocomposites

This study explores the dynamic deflection and vibrational analysis of a spherical shell, modeled as a football game ball, reinforced with graphene platelet nanocomposites (GPLs). The analysis leverages the Carrera Unified Formulation (CUF) for accurate and efficient modeling of the mechanical behavior of the shell under dynamic loads. CUF’s flexibility in adapting to complex geometries and material properties is utilized to represent the heterogeneous reinforcement of GPLs within the spherical shell structure. To enhance the reliability of the computational results, a hybrid artificial intelligence (AI) framework is implemented for result verification. This framework integrates Convolutional Neural Networks (CNNs) for spatial data representation with ReliefF feature selection to identify and prioritize influential variables. The hybrid AI system ensures robust predictive modeling, addressing the high-dimensional nature of the problem domain. The study also delves into the implications of graphene reinforcement on the ball’s performance, focusing on factors such as deformation under load, vibrational response, and stability thresholds. The results indicate that GPL reinforcement significantly improves the dynamic stability and stiffness of the spherical shell. Comparative analyses validate the efficiency of the CUF-based computational approach through mathematics benchmarks and AI-verified predictions. This interdisciplinary work highlights the potential of combining advanced computational mechanics, nanomaterials, and AI-driven verification in optimizing dynamic stability for applications in sports engineering and beyond.

Fractal configurations of zigzag hexagonal type coronoid molecules: Graph-theoretical modeling and its impact on physicochemical behavior

Abstract Coronene, a benzenoid compound, holds significant potential for applications in diverse fields, including organic chemistry, materials science, and pharmaceuticals. This study focuses on the structural analysis of Zigzag Hexagonal Coronene Fractal (ZHCF), a unique molecular configuration with significant implications for materials science and nanotechnology. Utilizing topological indices across two-dimensional chemical structure networks, we evaluate critical physicochemical properties of these molecules. Analytical expressions for a wide range of connection number-based topological descriptors are derived, enabling the prediction of properties such as entropy, enthalpy of vaporization, boiling point, and the acentric factor. The use of these mathematical tools provides a deeper understanding of the molecular connectivity and distribution patterns within the ZHCF framework, revealing insights into its stability and potential functionality. The results demonstrate how these indices can effectively capture the structural nuances of complex molecular graphs, aiding in the rational design of advanced nanomaterials with improved optical and electronic properties. This research not only showcases the predictive power of topological descriptors but also highlights the potential applications of coronoid-based structures in creating high-performance materials for various technological and scientific advancements. The findings pave the way for future exploration of coronoid structures in developing innovative solutions across diverse fields.

Cationic Ring-Opening Polymerization-Induced Self-Assembly (CROPISA) of 2-Oxazolines: From Block Copolymers to One-Step Gradient Copolymer Nanoparticles.

In recent years, polymerization-induced self-assembly (PISA) has emerged as a powerful method for the straightforward synthesis of polymer nanoparticles at high concentration. In this study, we describe for the first time the synthesis of poly(2-oxazoline) nanoparticles by dispersion cationic ring-opening polymerization-induced self-assembly (CROPISA) in n-dodecane. Specifically, a n-dodecane-soluble aliphatic poly(2-(3-ethylheptyl)-2-oxazoline) (PEHOx) block was chain-extended with poly(2-phenyl-2-oxazoline) (PPhOx). While the PhOx monomer is soluble in n-dodecane, its polymerization leads to n-dodecane-insoluble PPhOx, which leads to in situ self-assembly of the formed PEHOx-b-PPhOx copolymers. The polymerization kinetics and micellization upon second block formation were studied, and diverse nanoparticle dispersions were prepared, featuring varying block lengths and polymer concentrations, leading to dispersions with distinctive morphologies and physical properties. Finally, we developed a single-step protocol for the synthesis of polymer nanoparticles directly from monomers via gradient copolymerization CROPISA, which exploits the significantly greater reactivity of EHOx compared to that of PhOx during the statistical copolymerization of both monomers. Notably, this approach provides access to formulations with monomer compositions otherwise unattainable through the block copolymerization method. Given the synthetic versatility and application potential of poly(2-oxazolines), the developed CROPISA method can pave the way for advanced nanomaterials with favorable properties as demonstrated by using the obtained nanoparticles for stabilization of Pickering emulsions.

Cationic Ring‐Opening Polymerization‐Induced Self‐Assembly (CROPISA) of 2‐Oxazolines: From Block Copolymers to One‐Step Gradient Copolymer Nanoparticles

AbstractIn recent years, polymerization‐induced self‐assembly (PISA) has emerged as a powerful method for the straightforward synthesis of polymer nanoparticles at high concentration. In this study, we describe for the first time the synthesis of poly(2‐oxazoline) nanoparticles by dispersion cationic ring‐opening polymerization‐induced self‐assembly (CROPISA) in n‐dodecane. Specifically, a n‐dodecane‐soluble aliphatic poly(2‐(3‐ethylheptyl)‐2‐oxazoline) (PEHOx) block was chain‐extended with poly(2‐phenyl‐2‐oxazoline) (PPhOx). While the PhOx monomer is soluble in n‐dodecane, its polymerization leads to n‐dodecane‐insoluble PPhOx, which leads to in situ self‐assembly of the formed PEHOx‐b‐PPhOx copolymers. The polymerization kinetics and micellization upon second block formation were studied, and diverse nanoparticle dispersions were prepared, featuring varying block lengths and polymer concentrations, leading to dispersions with distinctive morphologies and physical properties. Finally, we developed a single‐step protocol for the synthesis of polymer nanoparticles directly from monomers via gradient copolymerization CROPISA, which exploits the significantly greater reactivity of EHOx compared to that of PhOx during the statistical copolymerization of both monomers. Notably, this approach provides access to formulations with monomer compositions otherwise unattainable through the block copolymerization method. Given the synthetic versatility and application potential of poly(2‐oxazolines), the developed CROPISA method can pave the way for advanced nanomaterials with favorable properties as demonstrated by using the obtained nanoparticles for stabilization of Pickering emulsions.

Red-light Emitting Orthogonally Trireactive Gold Nanoclusters for the Synthesis of Multifunctionalized Nanomaterials.

For the development of highly multifunctionalized nanomaterials, the introduction of functional molecules on gold nanoclusters containing thiols preinstalled with connecting groupsconstitutes a promising approach. However, the uniform introduction of multiple connecting groups while avoiding side reactions is a challenging task. Herein, the synthesis of gold nanoclusters (ca. 1nm) coated with thiol peptides bearing azido, amino, and aminooxy groups is reported. These nanoclusters emit red-light before and after the functionalization, enabling application to cell imaging. A detailed structure analysis using transmission electron microscope and X-ray absorption spectroscopy reveals the formation of Aun(SR)m nanoclusters as promising motifs for red-light emission. The sequential modification of the trireactive nanoclusters with RGD (Arg-Gly-Asp) peptides, metal chelators, and anticancer drugs via [3+2] cycloaddition, oximation, and amidation reactions at each functional group furnish red-light-emissive nanomaterials exhibiting remarkable toxicity against A549 human lung cancer cells. Integration of the multiligation chemistry and gold nanocluster engineering pave the way toward the development of advanced multifunctional nanomaterials for biological applications.

Exploring the role of edges in surface functionalization and stability of plasma-modified carbon materials: Experimental and DFT insights

Effective surface functionalization of carbon nanomaterials plays a crucial role in various applications. We investigated the impact of edges on surface functionalization and stability of oxygen-modified carbon materials using a combination of experimental techniques and Density Functional Theory (DFT) insights. Graphenic paper, highly oriented pyrolytic graphite (HOPG), and graphenic flakes were employed as model systems, with oxygen plasma treatment (generator power 100 W, oxygen pressure 0.2 mbar, exposure time 6 – 300 s) serving as the modification method. Surface morphology and chemical composition were characterized using scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), and Raman spectroscopy. The results revealed the introduction of oxygen functional groups on the investigated carbon surfaces (up to 20 at % by XPS) whereas; the structural integrity of the materials remained intact upon plasma modification (SEM, Raman). Work function was used as a sensitive parameter for monitoring the surface changes (increase by ∼1.4 eV, 1.3 eV, and 1 eV for graphenic paper, HOPG, and graphenic flakes, respectively) while time-dependent measurements revealed distinct kinetic processes governing the decay of functionalization, highlighting the role of surface defects in post-plasma processes. DFT calculations provided molecular-level insights into the surface processes, elucidating the mechanisms underlying the diffusion of hydroxyls, their recombination, and water desorption. Since the calculated activation barrier for recombination on basal graphenic planes (∼1.0 eV) and edges (∼5.5 eV) are distinctly different, it can be thus concluded that the persistent functionalization is due to the surface edges. Our findings contribute to a deeper understanding of surface modification processes of carbon materials and offer rationales for the design of advanced functional nanomaterials with tailored surface properties.

Plant-based protein amyloid fibrils: Origins, formation, extraction, applications, and safety.

Amyloid fibrils (AFs) are highly ordered nanostructures formed through the self-assembly of proteins under specific conditions. Due to their unique properties, AFs have garnered significant attention as biomaterials over the past decade. Nevertheless, the increasing reliance on animal proteins for AFs production raises sustainability concerns, highlighting the need for a transition to plant-based proteins as more environmentally friendly feedstocks. This review summarizes the conditions, mechanisms, and factors influencing the fibrillisation of over 20 plant-based protein amyloid fibrils (PAFs). The effectiveness of enzymatic extraction and membrane separation for isolating PAFs was also evaluated. Additionally, the review discusses the potential for enhancing PAFs' suitability through cross-linking with external agents. In the future, PAFs may be developed as advanced nanomaterials for a range of applications, including food hydrogels, cell-cultured meat scaffolds, and food detection sensors. However, thorough investigation of safety concerns and process improvements remain the primary challenges for the development of PAFs.

Recent developments in melamine detection: Applications of gold and silver nanostructures in colorimetric and fluorometric assays

The purity of milk, traditionally regarded as a symbol of health and nourishment, has been undermined by the alarming issue of melamine (MLM) adulteration. This nitrogen-rich compound is illicitly introduced to falsely enhance protein content, posing significant health risks. Traditional detection methods are often labor-intensive, time-consuming, or require expensive equipment. In response, researchers have developed colorimetric detection techniques to efficiently screen milk for MLM contamination. These methods are particularly promising due to their ease of preparation, rapid detection, high sensitivity, and capability for naked-eye detection. Furthermore, the unique optical properties of advanced nanomaterials have facilitated fluorometric detection, wherein the presence of contaminants induces detectable changes in fluorescence intensity or wavelength. This study offers an in-depth review of recent advancements in colorimetric and fluorometric probes based on silver (Ag) and gold (Au) nanostructures, exploring their application in food analysis. It delves into the underlying sensing mechanisms of these probes, showcasing their efficacy in detecting food contaminants. Despite the numerous advantages of Ag and Au nanostructure-based probes, challenges remain, particularly in addressing the complexity of food matrices, achieving simultaneous detection of multiple analytes, and mitigating interference from testing conditions. Additionally, this review highlights the emergence of immunoassay-based sensors, noting that many commercially available MLM testing kits utilize ELISA and LFIA platforms. For the first time, a comprehensive list of MLM testing devices and assay kits is presented, accompanied by key findings from recent studies and recommendations for future research directions.

Application of Fluorescence- and Bioluminescence-Based Biosensors in Cancer Drug Discovery.

Recent advances in drug discovery have established biosensors as indispensable tools, particularly valued for their precision, sensitivity, and real-time monitoring capabilities. The review begins with a brief overview of cancer drug discovery, underscoring the pivotal role of biosensors in advancing cancer research. Various types of biosensors employed in cancer drug discovery are then explored, with particular emphasis on fluorescence- and bioluminescence-based technologies such as FRET, TR-FRET, BRET, NanoBRET, and NanoBiT. These biosensors have enabled breakthrough discoveries, including the identification of Celastrol as a novel YAP-TEAD inhibitor through NanoBiT-based screening, and the development of TR-FRET assays that successfully identified Ro-31-8220 as a SMAD4R361H/SMAD3 interaction inducer. The integration of biosensors in high throughput screening and validation for cancer drug compounds is examined, highlighting successful applications such as the development of LATS biosensors that revealed VEGFR as an upstream regulator of the Hippo signaling pathway. Real-time monitoring of cellular responses through biosensors has yielded invaluable insights into cancer cell signaling pathways, as demonstrated by NanoBRET assays detecting RAF dimerization and HiBiT systems monitoring protein degradation dynamics. The review addresses challenges linked to biosensor applications, such as maintaining stability in complex tumor microenvironments and achieving consistent sensitivity in HTS applications. Emerging trends are discussed, including integrating artificial intelligence and advanced nanomaterials for enhanced biosensor performance. In conclusion, this review offers a comprehensive analysis of fluorescence- and bioluminescence-based biosensor applications in the dynamic cancer drug discovery field, presenting quantitative evidence of their impact and highlighting their potential to revolutionize targeted cancer treatments.

Exploring Anisotropy Contributions in Mn x Co1-x Fe2O4 Ferrite Nanoparticles for Biomedical Applications.

Designing well-defined magnetic nanomaterials is crucial for various applications, and it demands a comprehensive understanding of their magnetic properties at the microscopic level. In this study, we investigate the contributions to the total anisotropy of Mn/Co mixed spinel nanoparticles. By employing neutron measurements sensitive to the spatially resolved surface anisotropy with sub-Å space resolution, we reveal an additional contribution to the anisotropy constant arising from shape anisotropy and interparticle interactions. Our findings shed light on the intricate interplay among chemical composition, microstructure, morphology, and surface effects, providing valuable insights for the design of advanced magnetic nanomaterials for AC biomedical applications, such as cancer treatment by magnetic fluid hyperthermia.

Metal‐Organic Frameworks (MOFs) for Glucose Sensing: Advancing Non‐Invasive Detection Strategies in Diabetes Management

AbstractEffective glucose monitoring is critical for managing diabetes and preventing its associated complications. While commercial glucose monitoring devices predominantly rely on blood samples, emerging research focuses on detecting glucose in alternative biofluids, harnessing advanced nanomaterials. Among these, Metal‐Organic Frameworks (MOFs), composed of metal ions and organic ligands, have garnered significant attention due to their unique properties, including tunable porosity, high surface area, and abundant active sites conducive to glucose interaction. MOFs present a versatile platform for glucose sensing, offering potential in both enzymatic and non‐enzymatic detection methods. This review delves into the recent advancements in MOFs‐based electrochemical glucose sensors, providing a comprehensive analysis of various MOFs and their composites as electrode materials. The discussion highlights the structural attributes, functionalization strategies, and electrochemical performance of MOFs in glucose sensing, emphasizing their role in the development of next‐generation, non‐invasive glucose monitoring technologies. The review provides a comprehensive overview on the application of MOFs and MOFs‐based composites in both enzymatic and non‐enzymatic electrochemical‐based glucose sensing and highlights the synthesis, mechanism, functionalization and development in the detection strategy of MOFs in glucose sensing.

Liquid Phase Exfoliation of Protein Parent Crystals into Nanosheets and Fibrils Based on Orthogonal Supramolecular Interactions.

Proteins are attractive building blocks for fabricating diverse and precise nanomaterials. However, the facile fabrication of multidimensional artificial assemblies is highly challenging. Here, inspired by the large-scale production technique of inorganic nanomaterials, we demonstrate the application of liquid phase exfoliation (LPE) on native protein ConA by the design of synthetic ligands. These ligands provide distinct in-plane and out-of-plane supramolecular interactions, allowing the generation of multidimensional architectures based on the same protein by dissociating a single interaction in solution, including 3D porous protein crystals, 2D sizable nanosheets, and 1D fibrils. Importantly, the exfoliated 2D sheets were dozens of times larger than the self-assembled nanosheets, resulting in a dramatic enhancement of the intrinsic bioactivity of the building blocks by receptor clustering and less endocytosis. These findings enable the successful application of LPE on biomacromolecules and open up an alternative avenue to generate advanced multidimensional nanomaterials, without the need for complex protein design and careful adjustment of self-assembly conditions.

Valorization of Protein Materials Through Mechanochemistry and Self-Assembly.

The concept of combining mixing of solids by milling (a type of mechanochemistry) with aqueous self-assembly provides interesting possibilities for energy efficient production of advanced nanomaterials. Many proteins are outstanding building blocks for self-assembly, a prominent example being the conversion of proteins into protein nanofibrils (PNFs) - a structure related to amyloid fibrils. PNFs have attractive mechanical properties and have a tendency to form ordered materials. They are accordingly of interest as materials for bioplastics and potentially also for more high-tech applications. In this concept article we highlight our effort on valorization of such proteins with hydrophobic organic compounds such an organic dyes and drug molecules, by developing scalable methodology combining mechanochemistry and self-assembly. Compared to more established methodology, mechanochemical methodology is a valuable complement as it allows potential scalable production of hybrids between e. g. proteins and highly hydrophobic compounds - a class of hybrid material that is difficult to access by other means. This may allow for development of sustainable processes for fabrication of advanced protein-based materials derivable from renewable source materials.

Advanced Nanomaterials for Cancer Therapy: Gold, Silver, and Iron Oxide Nanoparticles in Oncological Applications.

Cancer remains one of the most challenging health issues globally, demanding innovative therapeutic approaches for effective treatment. Nanoparticles, particularly those composed of gold, silver, and iron oxide, have emerged as promising candidates for changing cancer therapy. This comprehensive review demonstrates the landscape of nanoparticle-based oncological interventions, focusing on the remarkable advancements and therapeutic potentials of gold, silver, and iron oxide nanoparticles. Gold nanoparticles have garnered significant attention for their exceptional biocompatibility, tunable surface chemistry, and distinctive optical properties, rendering them ideal candidates for various cancer diagnostic and therapeutic strategies. Silver nanoparticles, renowned for their antimicrobial properties, exhibit remarkable potential in cancer therapy through multiple mechanisms, including apoptosis induction, angiogenesis inhibition, and drug delivery enhancement. With their magnetic properties and biocompatibility, iron oxide nanoparticles offer unique cancer diagnosis and targeted therapy opportunities. This review critically examines the recent advancements in the synthesis, functionalization, and biomedical applications of these nanoparticles in cancer therapy. Moreover, the challenges are discussed, including toxicity concerns, immunogenicity, and translational barriers, and ongoing efforts to overcome these hurdles are highlighted. Finally, insights into the future directions of nanoparticle-based cancer therapy and regulatory considerations, are provided aiming to accelerate the translation of these promising technologies from bench to bedside.

Recent Progress and Applications of Advanced Nanomaterials in Solid-Phase Extraction.

Sample preparation maintains a key bottleneck in the whole analytical procedure. Solid-phase sorbents (SPSs) have garnered increasing attention in sample preparation research due to their crucial roles in achieving high clean-up and enrichment efficiency in the analysis of trace targets present in complex matrices. Novel nanoscale materials with improved characteristics have garnered considerable interest across different scientific disciplines due to the limited capabilities of traditional bulk-scale materials. The purpose of this review is to offer a thorough summary of the latest developments and uses of SPSs in preparing samples for chromatographic analysis, focusing on the years 2020-2024. The techniques for preparing SPSs are examined, such as metal-organic frameworks (MOFs), covalent organic frameworks (COFs), carbon nanoparticles (CNPs), molecularly imprinted polymers (MIPs), and metallic nanomaterials (MNs). Examining the pros and cons of different extraction methods, including solid-phase extraction (SPE), magnetic SPE (MSPE), flow-based SPE (FBA-SPE), solid-phase microextraction (SPME), stir-bar sorptive extraction (SBSE), and dispersive SPE (DSPE), is the main focus. Furthermore, this article presents the utilization of SPE technology for isolating common contaminants in various environmental, biological, and food specimens. We highlight the persistent challenges in SPSs and anticipate future advancements and applications of novel SPSs.

Modification of MWCNTs with Bi2WO6 nanoparticles targeting IL-1β and NLRP3 inflammasome via augmented autophagy.

This study reports the facile hydrothermal synthesis of pure Bi2WO6 and Bi2WO6\MWCNTs nanocomposite at specific molar ratio 1:2.5 of Bi2WO6:MWCNTs and elucidates their role in modulating the NLRP3 inflammasome pathway via autophagy induction. Comprehensive characterization techniques, including XRD, Raman, UV.Vis PL,FESEM,EDS and TEM, revealed the successful incorporation of MWCNTs into the Bi2WO6 structures, leading to enhanced crystattlinity, reduced band gap energy (2.4 eV) suppressed charge carrier recombination and mitigated nanoparticles aggregation. Notably, the reduced band gap facikitaed improved visible light harvesting, a crucial attribute for photocatalytic applications. Significantly, the nanocompsoite exhibited a remarkable capacity to augment autophagy in bone marrow-derived macrophages (BMDMs), consequently down-regulating the NLRP3 inflammasom activation and IL-1β secretion upon LPS and ATP stimulation. Immunofluorescence assays unveiled increased co-localization of LC3 and NLRP3, suggestion enhanced targeting of NLRP3 by autophagy. Inhibition of autophagy by 3-MA reversed these effects, confirming the pivotal role of autophagy induction. Furthermore, the nanocomposite attenuated caspase-1 activation and ASC oligomerzation, thereby impeding inflammasome assembly. Collectively, these findings underscore the potential of Bi2WO6\MWCNTs nanocompsite as a multifaceted therapeutic platform, levering its tailored optoelectronic properties and sbility to modulate the NLRP3 infalmmasome via autophagy augmentation. This work covers the way for the development of advanced nanomaterials with tunable functionalities for combating inflammatory disorders and antimicrobial applications.