Abstract Seasonal variation in total inorganic carbon (TCO 2 ) in surface waters of the western North Pacific (137°–152°E) subtropical gyre was analyzed on the basis of measurements of TCO 2 and partial pressure of CO 2 ( p CO 2 sw). The controlling processes including vertical mixing, horizontal advection, and net air–sea CO 2 transport, as well as biological activity, were quantified. The seasonal increase in normalized TCO 2 (NTCO 2 ) from autumn to winter, ranging from 19 to 37 μmol kg −1 in the northern part of the subtropical gyre between 24°N and 30°N, was predominantly accounted for by the upward supply of TCO 2 due to enhanced vertical mixing. The contribution of horizontal advection, estimated from monthly meridional NTCO 2 distributions and the monthly advection field of the Meteorological Research Institute (MRI)'s 3D-ocean general circulation model, was insignificant. Analyses of the mixed-layer NTCO 2 budget revealed that biological activity was playing an important role in the decrease in surface NTCO 2 from winter to summer. Annual net community production reached 48±19 gC m −2 between 24°N and 30°N, and 19±16 gC m −2 between 15°N and 23°N.