Advanced Treatment Methods Research Articles

2155-PUB: Diabetic Foot Ulcer Regeneration Platform Based on 4D Bioprinting Technology

Diabetic foot ulcers have a significantly negative impact on patients and are highly prone to contamination, which can lead to amputation. The best possible wound management treatment was essential for diabetic foot ulcers. However, since 4D bio printing is the best way to recapitulate complex and functional human tissue and organ, we have developed the therapeutic 4D bio printer called INVIVO to use in skin regeneration. To find advanced treatment method particularly for wound healing, we tested our own 4D bio printing system with autologous fat tissue for DFU treatment. The extracellular matrix (ECM) derived from Nano-fat consisting of supportive proteins, growth factors and cytokines, has been reported as an important player to provide efficient 3D environments during cell proliferation and differentiation. In the clinical study, we printed autologous ECM (MA-ECM) with bio-inks to apply onto the chronic wound site. Most of DFU patients showed significant reduction of wound size with distinct epithelization only after a week treatment. Therefore, we suggest 4D bio printing system with MA-ECM as an alternative method for dressing materials that successfully promote the mechanism of skin reconstruction for DFU treatment. Reduction in size of wound was measured using ImageJ NIH software and almost all the subjects revealed complete closure of wound maximum of 2∼5 weeks post MA-ECM membrane application. The present study provided a significantly higher healing rate of the subjects and the primary outcome of our study complete epithelization with wound closure was achieved. The autologous fat by nature is loaded with adipocytes and growth factors which could have played a vital role in the healing of non-healing diabetic wounds. Therefore, we suggest 4D bio printing system with MA-ECM as an alternative method for chronic wound healing that successfully promote the mechanism of skin reconstruction for DFU treatment. Disclosure J. Kim: None.

Application of advanced oxidation processes followed by different treatment technologies for hospital wastewater treatment

Hospital effluents are the sources for complex high percentage of micropollutants and till date no specific treatment have been investigated for the removal of pollutants. These effluents and their complex characteristics pose resistance to unit operation which undergoes in hospital wastewater treatment plants (WWTPs). In other words such complex contaminants are unable to removed completely through conventional treatment processes. The pharmaceutical residues entering into the environment may pose a serious threat to the water resources around the world. In past few years environmental scientists are more concerned towards the effluent generated from medical care facilities, community health centers and hospitals. In this study, seven multi-specialty hospitals with non-identical pre-treatment were selected for 3-aspects i.e. conventional wastewater characteristics, high priority pharmaceuticals and microbial analyses. The present work is to evaluate efficacy of advanced wastewater treatment methods with regard to removal of these 3-aspects from hospital effluents before discharge into a sewage treatment plant (STP). Based on test results, two out of seven treatment technologies i.e. MBR and CW effectively reducing conventional parameters and pharmaceuticals from secondary and tertiary treatments except regeneration of microbes were observed in tertiary level by these two treatments. The result also suggests that, MBR exhibited 100% elimination of ibuprofen, carbamazepine, frusemide while CW indicated 100% elimination of ofloxacin. Furthermore, two combinations of advanced oxidation process viz. ozonation (O3) and peroxone process (O3–H2O2) were demonstrated to oxidize pharmaceuticals. The operational key factors for AOP involve pH, O3 supply, contact time, and H2O2 concentrations. Based on pharmaceuticals reduction efficiencies, the conventional O3 alone is more efficient as compared to combine O3–H2O2 treatment. Hence results confirmed that, MBR coupled with ozone process proved as an optimal technology among the seven technologies for the pre-treatment of hospital effluent.

Ozonation of carbamazepine and its main transformation products: product determination and reaction mechanisms

Carbamazepine (CBZ) is a recalcitrant pharmaceutical often detected in wastewater and in the environment. CBZ can be removed from wastewater through advanced oxidation treatment methods such as ozonation. In this study, CBZ and its transformation product 1-(2-benzaldehyde)-(1H,3H)-quinazoline-2,4-dione (BQD) were ozonated, and the formation and transformation of their ozonation products were investigated using liquid chromatography coupled to ion trap mass spectrometry and high-resolution mass spectrometry as well as nuclear magnetic resonance (NMR). The main products, 1-(2-benzaldehyde)-4-hydro-(1H,3H)-quinazoline-2-one (BQM) and BQD were quantified using isolated standards and LC-UV. Of the original CBZ concentration, 74% was transformed into BQM and 83% of BQM was further transformed into BQD. Both products are more stable than CBZ and could still be detected after 240 min of ozonation. Another major product, 2,2′-azanediyldibenzaldehyde (TP225) was for the first time identified using NMR. Twelve further CBZ products were identified.

Research progress on mechanism and dosimetry of brainstem injury induced by intensity-modulated radiotherapy, proton therapy, and heavy ion radiotherapy.

Radiotherapy (RT) is an effective method for treating head and neck cancer (HNC). However, RT may cause side effects during and after treatment. Radiation-induced brainstem injury (BSI) is often neglected due to its low incidence and short survival time and because it is indistinguishable from intracranial tumor progression. It is currently believed that the possible mechanism of radiation-induced BSI includes increased expression of vascular endothelial growth factor and damage of vascular endothelial cells, neurons, and glial cells as well as an inflammatory response and oxidative stress. At present, it is still difficult to avoid BSI even with several advanced RT techniques. Intensity-modulated radiotherapy (IMRT) is the most commonly used therapeutic technique in the field of RT. Compared with early conformal therapy, it has greatly reduced the injury to normal tissues. Proton beam radiotherapy (PBT) and heavy ion radiotherapy (HIT) have good dose distribution due to the presence of a Bragg peak, which not only results in better control of the tumor but also minimizes the dose to the surrounding normal tissues. There are many clinical studies on BSI caused by IMRT, PBT, and HIT. In this paper, we review the mechanism, dosimetry, and other aspects of BSI caused by IMRT, PBT, and HIT.Key Points• Enhanced MRI imaging can better detect radiation-induced BSI early.• This article summarized the dose constraints of brainstem toxicity in clinical studies using different techniques including IMRT, PBT, and HIT and recommended better dose constraints pattern to clinicians.• The latest pathological mechanism of radiation-induced BSI and the corresponding advanced treatment methods will be discussed.

A Study on the Rational Requirement Area Estimation of Municipal Sewage Treatment Facilities Using Statistical Techniques

Objectives:In this study, a adjusted sewage & wastewater treatment capacity-required area estimation methodology was proposed through various data analysis for the required area of domestic sewage & wastewater treatment, the occupancy ratio of each sub-facility, and the treatment processing method. In addition, the reduction rate of the required area according to the application of the adjustment methodology presented in this study was evaluated quantitatively by comparing the actual area with the expected area.Methods:Statistical analysis was performed by collecting 654 data of more than 500 m<sup>3</sup>/day of treatment capacity in domestic sewage & wastewater treatment facilities. The 33 sewage & wastewater treatment facilities constructed by the LH sample groups were divided into functional areas and analyzed for occupancy rates by each facility.Results and Discussion:A capacity-required area regression model was developed for the collected each samples. As a result, the distributions of the regression model were divided into two clusters according to the analysis sample differences. It was analyzed that the estimated required area using the local government sample group was estimated to be relatively higher than the LH sample group. In this study, a adjusted capacity-required area model based on regression model derived from LH sample was proposed by considering green site ratio and treatment processing methods in sewage & wastewater treatment plant. The results are as follows: A = 15.638×Q<sup>0.7414</sup>×<i>α</i>×<i>β</i> (<i>α</i>: conversion factor for green site ratio, <i>β</i>: conversion factor for treatment processing method). Conclusions:Recently, the wastewater treatment facilities have been in decentralization and small scale, and the introduction of the advanced treatment method that requires a relatively small area such as MBR or SBR has been accelerating. Consequently, it could be expected that the methodology proposed in this study will save about 50~60% of the required area for wastewater treatment facilities.

Degradation of emerging contaminants by sono-Fenton process with in situ generated H2O2 and the improvement by P25-mediated visible light irradiation

Developing advanced treatment methods to minimize the release of emerging contaminants to natural water has become a matter of considerable interest. Sono-Fenton process was investigated to degrade bisphenol A (BPA) and sulfadiazine (SDZ). The H2O2 generated in situ was used as the exclusive source. Results showed that, the 400 kHz ultrasound is more efficient in creating homogeneous sono-Fenton than the 20 kHz apparatus due to the higher production of OH. Influence of Fe2+ was more remarkable on the degradation of hydrophilic SDZ, and its degradation kinetics was well fitted by two-stage kinetic model. However, the Fe2+ and H2O2 were unproductively wasted, which could not be improved by changing the dosing modes of Fe2+. The presence of P25 under visible light irradiation could significantly accelerate SDZ degradation at small amount of iron precursors, mainly via promoting the Fe2+/Fe3+ cycling by the photoelectrons. Moreover, SDZ degradation in sono-Fenton process was significantly inhibited at pH > 7, but the inhibition was very weak in P25-assisted sono-Fenton process. The presence of P25 also improved the mineralization. Three primary degradation pathways of SDZ degradation were proposed, including the attacking of the benzene ring, the oxidation of the amino group and the extrusion of SO2.

Study of Photocatalytic Properties of Ag/AgCl-Decorated Soybean Protein Knitting Fabric Against Acid Blue 260 Dye by Factorial Design

Photocatalytic materials have been the focus of several studies due to their excellent optical photoexcitation properties for waste treatment. However, their use promotes the generation of inorganic secondary waste, due to their nanometric grain size, which can cause several environmental hazards. The optimization and immobilization of such photocatalysts for treatment after the catalytic process have thus attracted much attention. In this work, Ag/AgCl nanoparticles were immobilized by hydrothermal treatment on soybean knitted fabric. The photocatalytic activity of the resulting Ag/AgCl-functionalized soybean knitted fabric was estimated based on the reduction of the concentration of Acid Blue 260 dye. The response surface factorial design methodology was applied, varying the Ag/AgCl concentration, nanocatalyst immobilization time, and hydrothermal process temperature, to analyze the individual effect of and interaction between the variables, besides establishing a mathematical model of the process. The photocatalytic results showed that addition of Ag/AgCl to the fabric favored its use and reuse for the degradation of the Acid Blue 260 dye. The mathematical model together with the response surfaces showed that the residence time in the hydrothermal bath was the most significant variable in terms of the dye reduction, favoring this advanced treatment method.

Comparison of pneumonia severity scores for patients diagnosed with pneumonia in emergency department.

Background & objectives:Sepsis due to pneumonia or pneumonia itself is one of the main causes of deaths in patients despite the advanced treatment methods. The optimal prognostic tool in pneumonia is still not clear. This study was aimed to compare the pneumonia severity scores and the possibility of using the new scores in patients who were diagnosed with pneumonia in the emergency department.Methods:Demographic data, laboratory and imaging results, confusion, elevated blood urea nitrogen, respiratory rate and blood pressure plus age ≥65 yr (CURB-65), pneumonia severity index (PSI), national early warning score (NEWS), NEWS-lactate (NEWS-L) scores, hospitalization, referral, discharge and 30-day mortality of patients who were diagnosed with pneumonia in emergency department were recorded.Results:A total of 250 patients were included in the study. The most successful score in predicted mortality was found to be NEWS-L. This was followed by NEWS, CURB-65 and PSI, respectively. Most successful scores in anticipation of admission to the intensive care unit were NEWS-L followed by NEWS. This was followed by CURB-65 and PSI scores, respectively. The most successful score in anticipation of hospital admission was NEWS-L, followed by NEWS, CURB-65 and PSI, respectively. There was a significant difference between all pneumonia severity scores of the patients who died and survived within 30 days. There was a significant difference between the scores of patients in intensive care unit (ICU) and service, compared to non-ICU patients.Interpretation & conclusions:NEWS-L score was found to be the most successful score in predicting mortality, ICU admission and hospitalization requirement. Both NEWS-L and NEWS scores can be used in determining the mortality, need for hospitalization and intensive care of the patients with pneumonia in the emergency department.

Methods of assessing the condition of filtering bleb after glaucoma surgery

Currently, there is a need to assess the condition of structures at the surgery site after glaucoma intervention due to the necessity to decide further treatment tactics after performed operations, as well as the possibility of developing more advanced methods of surgical treatment of glaucoma. Despite the fact that there are several methods of examining the filtration bleb (FB), some of them have both clear advantages and significant disadvantages. This article reviews the possibilities of using optical coherence tomography (OCT), ultrasound biomicroscopy (UBM), thermography, confocal microscopy, and the Hyperemia-3 program for assessing the structural and functional state of the FB.

Evaluation of a biological post-treatment after full-scale ozonation at a municipal wastewater treatment plant

To reduce the discharge of trace organic compounds into water bodies associated with potential toxic effects such as endocrine disruption, new advanced treatment methods are being investigated at several wastewater treatment plants (WWTPs). One of the most studied and already implemented technologies is ozonation. However, ozonation only partially oxidizes trace organic compounds (TrOC) and as a result, transformation products (TPs) with unknown properties can be formed. In order to minimise the risk of releasing unknown and potentially toxic TPs into surface water, it is recommended to install a biological post-treatment after ozonation. The aim of this study was to evaluate the efficiency of a moving bed reactor following ozonation in a full-scale plant. Different ozone dosages (zspec. = 0.3, 0.5, 0.7 mg O3/mgDOC) were investigated. To assess the biological activity of the post-treatment, the assimilable organic carbon (AOC) was determined in addition to the formed biomass. Furthermore, selected TrOC were analysed in parallel to monitor the ozonation efficiency at different ozone doses. In addition, estrogenic, androgenic as well as corresponding antagonistic effects were investigated after each treatment step using the A-YES and A-YAS assay. A non-target screening was performed to evaluate a trend analysis of formed TPs as well as their removal by the post-treatment procedure. The results proved the successful design of the biological post-treatment reactor by a constant biofilm development and reduction of the AOC. Endocrine effects were removed below the limit of detection (LOD) of 10 pg EEQ/L already after ozonation for all applied ozone doses. Antagonistic effects were not significantly reduced during ozonation and subsequent biological post-treatment. For this reason, further research is needed to evaluate different post-treatment technologies. The trend analysis from non-target screening data showed a reduction of about 95% of the number of formed TPs by the biological post-treatment. Consequently, an assessment of the biological activity and the elimination capacity of a certain biological post-treatment technique is thus possible by applying the AOC in combination with a non-target screening.

Reclamation of hospital secondary treatment effluent by sulfate radicals based–advanced oxidation processes (SR-AOPs) for removal of antibiotics

Nowadays, pollution of water resources by emerging resistant contaminants such as antibiotics is a growing issue. The limitations in conventional treatment methods for the removal of emerging contaminants intrigued the researcher to propose advanced treatment methods. Moreover, the high values of carbon-based compounds in wastewater and also treated effluent arise issues in treatment processes. Thus, this study aims to determine the removal of amoxicillin and ciprofloxacin from hospital effluent using an ultraviolet-activated persulfate-based advanced oxidation process. The effect of the study variables including persulfate concentration (2.5–10 mg L−1), pH(4–11), initial concentration of antibiotics (1,10 mg L−1) and reaction time (2.5–120 min) was investigated on the removal of antibiotics. Moreover, the chemical reaction kinetics and mineralization rate were determined and the interference effect of various ions was investigated. The results showed that the highest removal of ciprofloxacin and amoxicillin were observed to be respectively 99.9% and 99.26% at the reaction time of 60 min, initial concentration of 1 mg L−1and persulfate concentration of 10 mg L−1. The antibiotics removal rate increased as their initial concentration decreased Further, increase of the persulfate concentration and reaction time increased the removal rate. Degradation kinetics of ciprofloxacin and amoxicillin followed the pseudo-first-order equation. The use of persulfate did not significantly increase the sulfate and nitrate content of wastewater. Moreover, mineralization was not performed in the ultraviolet/persulfate process for both antibiotics. The results showed that the ultraviolet-activated persulfate-based advanced oxidation process can be used as an appropriate method for the removal of antibiotics in complicated aqueous media such as hospital effluent.

A case of a patient treated with percutaneous edge-to-edge mitral valve repair, percutaneous left atrial appendage occlusion and implantable cardioverter-defibrillator

The article presents the case of a 55-year-old woman who suffered from anterior myocardial infarction and chronic complications of the underlying disease, heart failure with reduced left ventricular ejection fraction, severe secondary mitral regurgitation, and paroxysmal atrial fibrillation. Due to the severity of symptoms, which persisted despite the optimal pharmacotherapy, after exclusion of reversible causes, the patient was qualified for different advanced percutaneous treatment methods. Within two years from the onset of the disease, three percutaneous procedures were performed: mitral valve correction with the MitraClip system, left atrial appendage occlusion using the Watchman system, and implantation of cardioverter-defibrillator.

The role and effect of FDG-PET/CT on patient management and restaging of bladder carcinoma.

To compare the diagnostic role of Florine-18 2-fluoro-2-deoxy-D-glucose-Positron emission tomography/computed tomography (FDG-PET/CT) in the restaging of bladder cancer with other radiological methods and to determine its effect on the treatment management of patients with bladder cancer. A total of 83 patients who showed suspicious lesions with radiologic methods and FDG-PET/CT images were enrolled in the study. Positive lesion sites were detected and compared in all imaging modalities. Positive lesions were confirmed by biopsy or serial radiological imaging. Furthermore, the rate of change of the management and treatment modalities of patients after FDG-PET/CT were noted. The most frequent metastasis was observed in lymphatic localizations in all imaging methods. Lymph node metastases was detected with FDG-PET/CT in 37/83 patients, with computed tomography in 28/80 patients, and with magnetic resonance imaging in 6/19 patients. Lymph node metastasis was detected most commonly in the pelvic region with all modalities. Following lymphatic localization in FDG-PET/CT and CT, metastases were found most frequently in the lung and bone regions. FDG-PET/CT also revealed 8 of the 12 local lesions that were detected by radiological methods and showed additional lesions in four patients that could not be demonstrated by radiological methods. FDG-PET/CT changed the treatment approaches in 34.9% (29/83) of patients. In 16 patients, it caused upstaging and commencement of advanced treatment methods. In 13 patients, malignancy was excluded in suspicious lesions and unnecessary advanced examination and treatment were avoided. FDG-PET/CT also caused three radical cystectomies, one partial cystectomy, and one urethrectomy in patients who had not undergone any operational procedure previously. Despite physiological urinary tract uptake, FDG-PET/CT is superior to other imaging modalities not only in distant localizations but also in evaluating pelvic lesions and lymph nodes. In addition, the use of FDG-PET/CT during the restaging process contributes to the management of maximum number of patients.

Improvement of Mechanical Properties of Plasma Sprayed Al2O3-ZrO2-SiO2 Amorphous Coatings by Surface Crystallization.

Ceramic Al2O3−ZrO2−SiO2 coatings with near eutectic composition were plasma sprayed using hybrid water stabilized plasma torch (WSP-H). The as-sprayed coatings possessed fully amorphous microstructure which can be transformed to nanocrystalline by further heat treatment. The amorphous/crystalline content ratio and the crystallite sizes can be controlled by a specific choice of heat treatment conditions, subsequently leading to significant changes in the microstructure and mechanical properties of the coatings, such as hardness or wear resistance. In this study, two advanced methods of surface heat treatment were realized by plasma jet or by high energy laser heating. As opposed to the traditional furnace treatments, inducing homogeneous changes throughout the material, both approaches lead to a formation of gradient microstructure within the coatings; from dominantly amorphous at the substrate–coating interface vicinity to fully nanocrystalline near its surface. The processes can also be applied for large-scale applications and do not induce detrimental changes to the underlying substrate materials. The respective mechanical response was evaluated by measuring coating hardness profile and wear resistance. For some of the heat treatment conditions, an increase in the coating microhardness by factor up to 1.8 was observed, as well as improvement of wear resistance behaviour up to 6.5 times. The phase composition changes were analysed by X-ray diffraction and the microstructure was investigated by scanning electron microscopy.

Epigenetics of suicidal behavior

Suicide is the second leading cause of death among young people and therefore being a serious global problem worldwide. The study of genetic and epigenetic factors in the development of suicidal behavior plays an important role in the development of advanced methods of diagnosis and treatment of this pathology. The role of hereditary factors in the development of suicidal behavior is estimated at 30–55 %, with a pronounced comorbidity with other psychopathologies. The study of genetic liability to suicidal behavior is based on molecular-genetic methods including association and linkage analyses, chip gene expression arrays, and genome-wide association studies. Published data identified multiple genes including those involved in the functioning of serotonergic (SLC6A4, TPH, 5-HT1A), hypothalamic-pituitary-adrenal systems (FKBP5) and polyamines (SAT and OATL1) associated with suicidal behavior. However, the diversity of interacting genetic loci complicates the interpretation of the development of a complex phenotype of pathology and prevents the association from being detected. To solve this problem and interpret the missing relationship between the environment and the genome, promising results were obtained from a study of epigenetic factors, which affected the expression of a number of candidate genes involved in brain functioning in suicidal behavior. The analysis of a brain obtained from suicide victims, representing a unique tool for the analysis of modified genomic processes, revealed a wide range of reprogramming patterns of DNA methylation in promoters of the genes of polyamine (OAZ1, OAZ2, AMD1, ARG2, SKA2), serotonergic (SLC6A4) and GABAergic (GABRA1) systems, HPA-axis (GR, NR3C1), tyrosine kinase (TrkB) receptors, brain-derived neurotrophic factor (BDNF). The role of histone modifications in distinct genes (Cx30, Cx43, TrkB.T1) and the expression of specific long noncoding RNAs and microRNAs in the development of suicidal behavior, which is promising for the development of diagnostic algorithms and target therapy, is discussed.

Fenton oxidation for effective removal of color and organic matter from denim cotton wastewater without biological treatment

Denim is a cotton fabric specifically used to manufacture jeans. Denim processing generates complex effluents with high levels of pumice stone, color and chemical oxygen demand (COD). There is therefore a need for advanced treatment methods to limit pollution of natural waters. Here, we hypothesized that Fenton oxidation, a method using Fe2+ and H2O2, could replace the traditional step of activated sludge treatment. We studied a daily composite sample at laboratory scale for preliminary settling, chemical settling and Fenton oxidation. We found that pumice stone can be effectively controlled by preliminary settling with partial COD removal and limited color removal. Chemical treatment improved COD removal, but color reduction still remained partial. Fenton oxidation decreased color below visual detection after 5 min and COD decreased to 110 mg/L after 30 min. These findings surpassed the performances of activated sludge treatment.

Modeling azo dye removal by sono-fenton processes using response surface methodology and artificial neural network approaches

Textile industry wastewaters, which cause serious problems in the environment and human health, include synthetic dyes, complex organic pollutants, surfactants, and other toxic chemicals and therefore must be removed by advanced treatment methods. Determination of appropriate treatment conditions for efficient use of advanced treatment methods is an important and necessary step. In the last thirty years, the Artificial Neural Network-Genetic Algorithm (ANN-GA) and Response Surface Methodology (RSM) have emerged as the most effective empirical modeling and optimization methods especially for nonlinear systems. Reactive Red 195 azo dyestuff was chosen as the target pollutant. The color removal efficiency was modeled and optimized as a function of Sono-Fenton conditions such as H2O2 dosage, Fe2+ dosage, initial pH value, ultrasound power, and ultrasound frequency, using ANN-GA and RSM. The generalization and predictive ability of these methods were compared using the results of the 46 experimental sets generated by the Box-Behnken design. The mean square errors for these models are 3.01612 and 0.00295, and the regression coefficients showing the superiority of ANN in determining nonlinear behavior are 0.9856 and 0.9164, respectively. In optimal conditions, the prediction errors with hybrid ANN-GA and RSM models are 0.002% and 3.225%, respectively.

Advanced Treatment of Meat Processing Wastewaters Using Microalgae

Introduction: Effluents of Meat processing are one of the untreated wastewaters containing high volumes of polluted components even after the primary and secondary treatments. The high cost of using the advanced treatment methods of the wastewaters has led the industry owners to release them in the nature without effective removal of nitrogen and phosphorus. In this study, an economic advanced method was presented for the first time for the treatment of meat processing effluents by producing valuable biomass. Methods: The recovery of wastewater components was studied for the growth of two profitable strains of Chlorella and Scenedesmus in three variations: real wastewater, normalized wastewater, and normalized wastewater with trace elements. The concentration of nitrate, ammonia, and phosphate was analyzed during the growth period. Results: Experimental data showed that normalized wastewater increased biological removal up to %78 in Scenedesmus and 64% in Chlorella. In addition, normalized wastewater containing trace elements increased biological removal up to %93 in Scenedesmus and 95% in Chlorella. The addition of phosphates reduced pH fluctuations and worked as a buffer. Conclusion: The meat wastewaters processing can provide a rich culture medium for cultivation of microalgae. The results of this research can present a novel promising and economic alternative way to remove nutrient pollutions in meat wastewaters processing.

Results of international meeting "Advanced methods of Treatment of Hip and Knee Pathology"

Results of international meeting "Advanced methods of Treatment of Hip and Knee Pathology"

Development And Optimization Of Biological Treatment Of Quarry Waters From Mineral Nitrogen In The Subarctic

The new concept of bioremediation of anthropogenic water bodies and quarry wastewaters treatment by phytoextraction and phytotransformation in the Subarctic conditions is presented. This technology is based on transformating the man-caused water reservoirs into nature-like marsh ecosystems. At the first stage, a new patented method for advanced waste treatment using floating bioplate was developed and implemented. After implementing the bioplate, the concentration of ammonium ions in water decreased by 53-90%, nitrate nitrogen reduced by 15-20%. At the second stage, the floating bioplate technology was modified into the highly efficient purificating marsh ecosystem, which allowed to cover the waterbody territory to the greatest possible extent. The technology is based on the creation of phytomats enabling in the accelerated mode to form plant blocks of three different types. They are aimed both at local grassing down, and at swamping deep and shallow areas of sediment ponds. In forming phytomats, two soil substitutional substrates (thermovermiculite and wood sawdust) and regionally-optimized assortment of 24 plant species are used. The proposed technology does not require energy, chemicals and soil components which are scarce in the region. The predominance of natural ecosystem processes in the formed phytocenoses allows to achieve maximum efficiency, and the use of available materials contributes to minimizing the costs of creating and maintaining the system. The introduction of this technology and formation of the artificial phytocenosis with the area of about 30% of the man-caused reservoirs territory made it possible to increase the efficiency of wastewater treating from mineral nitrogen compounds by 22%.