Background and Purpose: How to use steroid hormones as a solution and indicator in bone fractures and healing in sterilized or unsterilized male animals and even in female animals and animals that have a deficiency and disorder of steroid hormones. Research Method: Ten apparently healthy adult male rabbits were selected from the laboratory and were sterilized by scrotal method, and four holes with diameters of 1, 2, 3, and 4 mm were inserted on the tibia from the lateral side, and then into two The group was divided. Regarding the time evaluation of radiographs, which had a normal distribution, variance analysis tests were used at a significant level of 0.05. About the status of the steps that were in the ranking scale. Kruskal-Wallis test was used and statistical calculations were done using SPSS statistical program. Results: The gait status of rabbits in both groups improves significantly from day one to day 60. On the other hand, there is no significant difference between the two groups at different times and between different times in each group. Except for the first and seventh day, this difference is completely normal. In relation to the amount of callus formed in the two groups, there is a significant difference at some times. From the point of view of pathology, the assessment of the first and second groups indicated the occurrence of restoration and bone callus formation in both groups, but in comparison, the first group had a wider and more voluminous callus. The whole restoration process had more advanced stages.