Adult Male Rabbits Research Articles

Polydopamine grafting polyether ether ketone to stabilize growth factor for efficient osteonecrosis repair

This study examines the biocompatibility, osteogenic potential, and effectiveness of polyether ether ketone (PEEK) composites for treating osteonecrosis, seeking to establish a theoretical basis for clinical application. A range of PEEK composite materials, including sulfonated polyether ether ketone (SPEEK), polydopamine-sulfonated polyether ether ketone (SPEEK-PDA), bone-forming peptide-poly-dopamine-sulfonated polyether ether ketone (SPEEK-PDA-BFP), and vascular endothelial growth factor-poly-dopamine-sulfonated polyether ether ketone (SPEEK-PDA-VEGF), were constructed by concentrated sulfuric acid sulfonation, polydopamine modification and grafting of bioactive factors. The experiments involved adult male New Zealand rabbits aged 24–28 weeks and weighing 2.6–4 kg. The SPEEK-PDA-BFP possesses the smallest water contact angle, indicating the highest hydrophilicity, with its surface characterized by a rich density of clustered BFP particles. The SPEEK-PDA-BFP exhibits superior adhesion, proliferation, and differentiation capabilities, along with pronounced bacteriostatic effects, which are attributed to its dense particle clusters. The SPEEK-PDA-BFP facilitates the formation of regular and dense bone trabeculae. Comparative study on treating osteonecrosis with SPEEK-PDA-VEGF and SPEEK-PDA-BFP highlighted the superior formation of mature bone trabeculae and angiogenic protein CD31 around SPEEK-PDA-VEGF. The PEEK composite materials have good biocompatibility, osteogenic activity and bone repair activity. In particular, SPEEK-PDA-VEGF composite materials have the best effect on bone repair.

Dapagliflozin inhibits ferroptosis to improve chronic heart failure by regulating Nrf2/HO-1/GPX4 signaling pathway.

To study the effect of Dapagliflozin on ferroptosis in rabbits with chronic heart failure and to reveal its possible mechanism. Nine healthy adult male New Zealand white rabbits were randomly divided into Sham group (only thorax opening was performed in Sham group, no ascending aorta circumferential ligation was performed), Heart failure group (HF group, ascending aorta circumferential ligation was performed in HF group to establish the animal model of heart failure), and Dapagliflozin group (DAPA group, after the rabbit chronic heart failure model was successfully made in DAPA group). Dapagliflozin was given by force-feeding method. Echocardiography was used to assess cardiac function, HE staining to evaluate pathological changes in the heart, Prussia blue staining to observe iron ions in myocardial tissue, and enzyme-linked immunosorbent assay (ELISA) to determine serum levels of the inflammatory factors interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) at the end of week 12 and/or the end of week 16. The oxidative stress related indexes of malondialdehyde (MDA), superoxide dismutase (SOD) and superoxide dismutase (GSH-Px) in serum were quantitatively analyzed by colorimetry. Protein expression levels of nuclear factor E2-related factor 2(Nrf2), heme oxygenase-1(HO-1), glutathione peroxidase 4(Gpx4) were detected by Western blot. In animals with chronic heart failure, Dapagliflozin improved cardiomyocyte hypertrophy, degeneration and necrosis. Dapagliflozin increased serum GSH-Px and SOD levels and decreased IL-1β, IL-6, TNF-α and MDA levels (P < 0.05) in a rabbit model of heart failure. Dapagliflozin also decreased cardiac iron ion levels and increased Nrf2, HO-1 and GPX4 protein expression. Dapagliflozin can improve heart failure by inhibiting oxidative stress and ferroptosis, and its mechanism may be related to the regulation of Nrf2/HO-1/GPX4 signaling pathway.

MORPHOLOGICAL ASPECTS OF POST-TRAUMATIC LOWER JAW REMODELING IN THE SETTING OF OSTEOPLASTY

Bone tissue regeneration remains a complex and crucial challenge for modern medicine. The unique properties of chitosan, its effects on bone regeneration in the maxillofacial region, as well as the underlying mechanisms and dynamics, remain poorly understood and warrant further investigation. Aim. The aim of the study is to determine the dynamics of morphological, radiological, and lectin-histochemical characteristics of bone-ceramic regenerate after the transplantation of octacalcium phosphate with the addition of chitosan acetate with ampicillin into an experimental defect in the rabbit mandible. Materials and methods. Adult male rabbits aged 6-7 months and weighing 2.5-3 kg were used for the study. The control group consisted of animals with a bone defect that healed under a blood clot. The experimental group consisted of rabbits in which the bone defect was filled with an osteotropic material containing octacalcium phosphate, chitosan acetate, and ampicillin (OCР-N-Chitosan-Ampicillin). Post-traumatic bone tissue status within the defect area was monitored for 84 days. The following methods were employed: bone defect modeling, assessment of jaw macrostructure, radiographic examination, radiovisiography, examination of bone sections under a microscope, and lectin-histochemical analysis of decalcified bone sections. Results. Examination of the macrostructure of the experimental bone defect in the rabbit mandible after implantation of material with octacalcium phosphate, chitosan acetate, and ampicillin (OCР-N -Chitosan-Ampicillin) revealed numerous regenerative changes that occurred after the injury and correlated with the data of radiographic and radiovisiographic examinations. Microscopic examination of bone sections allowed us to establish the phased nature of the dynamics of the studied regenerative changes and the composition of the regenerate. Conclusions. It was established that in the experimental group of animals in which defect repair was performed using OCP-N-Chitosan-Ampicillin material, a shortening of the reactive phase of the post-traumatic inflammatory process was observed, which ensured the active onset of osteoregeneration. The use of this combination of drugs showed accelerated reparative regeneration of the mandible and adjacent mucosa.

Morphological characteristics of regeneration of the lower jaw bone in an experiment using natural collagen with lincomycin

This study aimed to investigate the dynamic changes in the histo­morphological architecture of bone-ceramic regeneration following the transplantation of natural collagen combined with lincomycin into an experimental defect of the rabbit mandible. 45 adult male rabbits aged 6-7 months and weighing 2.5-3 kg were used for the study. 20 animals were in control group and 20 animals in experimental. An additional 5 intact animals were used to study the normal structure of bone tissue in the studied area of the lower jaw. The control group consisted of animals with a bone defect that healed under a blood clot. The experimental group consisted of rabbits with the bone defect filled with natural collagen, with simultaneous intramuscular injections of lincomycin at a dose of 12 mg/kg body weight once a day for 6 days (Col-C-lincomycin). Post-traumatic bone tissue status within the defect area was monitored for 84 days. Ultrastructural changes were studied using scanning electron microscopy. To determine changes in the composition in the bone regeneration, three parameters were calculated – the relative volume of: 1) bone tissue, 2) osteoplastic material and 3) connective tissue in the bone regeneration. The data were analyzed using the Student's t-test, and a difference at p&lt;0.05 was considered statistically significant. The research of the surface topography of the experimental bone defect in the rabbit mandible, following the implantation of Col-C material in combination with lincomycin, revealed numerous regenerative changes that occurred after injury and correlated with the dynamic changes in the relative volume of bone tissue, osteoplastic material, and connective tissue within the regeneration. Morphometric analysis of the relative volume of the constituent components of the regeneration in the experimental defect revealed a phased nature of the dynamics of the observed changes. The osteocyte lacuno-canalicular system that formed after material implantation acquired characteristics of typical structure. Foci of incomplete osteogenesis were not visualized. Unlike the control group, after the application of Col-C material in combination with lincomycin, the majority of osteons in the regeneration near the outer bone plate did not differ from the typical structure of the maternal bone in terms of both its structure and geometry.

Histological and Histomorphomitrical Evaluation of Rabbit Oral Cavity Wound Healing by Feeding Absorbable Amino Acid

Background: Traumatic oral ulcer is well-circumscribed, depressed lesion with an epithelial defect that is covered by a fibrin clot, resulting in a yellow-white appearance, occurs due to chemical, mechanical or thermal injury to oral mucosal end with painful erosion. Amino acids are necessary for wound healing because they promote the growth of connective tissue as well as the activation and proliferation of fibroblasts. Objective: evaluation the effect of systemic application of amino acid collection (oral intake) in treatment of traumatic oral ulceration over selected time by histological and histomorophometric assessment of soft tissue healing. Patients and Methods: 20 adult male rabbits that weight about 700-900 Kg and age about (6-8) months where used in this experimental study. The traumatic ulcer created with (8mm) diameter, and (1mm) by surgical round diamond bur in the right cheek mucosa, then divided the groups in two groups,10 rabbits for control group that left healed normally , and 10 rabbits for experimental group that daily used mixture of amino acids systemically through mixing with water for one month. The animals were sacrificed along 3 and 7 days healing periods and the species examined histologically after histological preparation of the traumatic ulcer. Results: Histological and histomorphometeric findings showed decreased inflammation, accelerated reepithelization of ulcer surface, better angiogenesis, and promoted remodeling of the extracellular matrix resulting with enhanced tissue maturation and complete healing in all study groups than in the control group. Conclusion: the chemical medicament that represented by systemic application of amino acid effective in accelerating the healing of traumatized ulcers in experimental group than that in control group by accelerated cell proliferation and mucosa reepithelization. Keywords: Traumatic ulcers, amino acid, reepithelization.

Regeneration process of severed rabbit common calcanean tendons influenced by external compression

BackgroundLimited research has focused on the correlation between an external compression and the regeneration of ruptured Achilles tendons. The aim of this study was to evaluate the influence of a constricted paratenon with external compression on the regeneration process of separated rabbit common calcanean tendon stumps.MethodsA transection, establishing a 4 mm gap, was created in the right common calcanean tendon of 24 young adult male New Zealand white rabbits. The animals were assigned to two groups: In the control group, only received cast immobilization. In the constricted paratenon (CP) group, the rabbits had a local 3-dimensional printed clasp applied to mimic external compression and same cast immobilization as the control group. Morphologic, histologic and immunohistochemistry examinations were performed at 2 and 4 weeks postoperative.ResultsSeparated tendon stumps were connected by novel granulated tendon fibrils in the control group. However, the regenerated tendon fibrils appeared insufficient in the CP group, the tendon length and the adhesion grade of the CP group was significantly larger than that of the control group at 4 weeks (P < 0.05, P = 0.030). Disorganized collagen and round-shaped fibroblasts were demonstrated in the CP group. A prolonged expression of proliferating cell nuclear antigen (PCNA) and lower intensity in clusters of differentiation 146 (CD146) were also shown in the CP group. A prolonged existence of the vascular endothelial growth factor (VEGF) and lesser intensity of the transforming growth factor-beta 1 (TGF-β1) were confirmed within this group. Furthermore, the CP group’s expression had less collagen I than that of the control group at 4 weeks.ConclusionsSufficient regeneration can be obtained, even though there is an obvious gap between severed rabbit common calcanean tendon stumps. However, constricted paratenons with external compression can negatively influence the intrinsic regeneration process of the tendon fibrils and promotes the disorganization of regenerated collagen.

Silymarin attenuates oxidative stress and pathological changes induced by salinomycin in adult male rabbits

Feed additives are used in animal husbandry to prevent diseases and enhance growth rates. Salinomycin sodium (SAL) is an approved feed additive for rabbit fattening diets, typically administered at a dosage of 20–25 mg/kg feed with a 5-day withdrawal period. However, the misuse or abuse of SAL can result in toxicity in rabbits. This study aimed to evaluate the adverse effects of SAL administration in different organs for the first time and the protective role of silymarin in rabbits. Seven groups of rabbits were randomly allocated. The control group received no additives. The second and third groups received rations with 20 mg SAL/kg and 40 mg SAL/kg, respectively. The fourth and fifth groups received the same SAL doses with silymarin at 6.5 mg/kg body weight (bw) and 13 mg/kg bw, respectively. The sixth and seventh groups were administered silymarin alone at 6.5 mg/kg bw and 13 mg/kg bw, respectively. Rabbits were euthanized and slaughtered on day 29 following the Halal method. SAL at 20 mg/kg ration caused mild to moderate pathological changes compared to the control group, while 40 mg SAL/kg ration induced severe pathological changes. However, silymarin supplementation, alone or with SAL, improved organs histology. Both silymarin doses (6.5 mg/kg bw and 13 mg/kg bw) mitigated SAL's harmful effects. In conclusion, this study highlights the potential detrimental effects of SAL toxicity on different organs in rabbits. The supplementation of silymarin reduced and prevented these hazardous effects. These findings emphasize the promising role of silymarin as a protective agent against SAL-induced toxicity in rabbits.

Histological Determination of Cinnamon and Olive Oil Extract on Traumatic Oral Ulcer in Laboratory Rabbit

Background: The mucosa of oral cavity is the mucous membrane which covers the tissues of the mouth cavity. In order to repair damage from a local aggressor, many cell strains and their byproducts work together throughout the vital physiological process of wound healing. This process culminates in tissue repair and starts relatively early in the inflammatory phase. . The supplements of cinnamon and olive oil can dramatically raise blood levels of antioxidants while lowering those of inflammatory indicators like C-reactive protein. Objective: The aim of the study was to determine the local histological effect of topical application of cinnamon and olive oil extract on the rabbit oral mucosa Patients and Methods: 20 adult male rabbits that weight about 700-900 Kg and age about (6-8) months where used in this experimental study. Ulcer induction: Prior to the creation of the ulcers, rats were fixed on their backs and all animals were anaesthetized and induction the ulcer with round filter papers 5.5 mm in diameter were soaked in 15 ml of 50% acetic acid. In order to create round ulcer, an acid-soaked filter paper was pressed onto the right buccal mucosa for 60 seconds. Then divided the groups according to the healing time with 10 rabbits as a control group left healed normally and 10 rabbits as an experimental group that daily used mixture of cinnamon extract and olive oil ready extract topically applied on the traumatic ulcer. The animals were sacrificed along three- and seven-days healing periods and then prepared H&amp;E stain for analyzed the results. Results: In comparison to the control group, the histological results of oral ulcers that were created and treated with a daily application of a herbal mixture consisting of cinnamon extract and olive oil extract showed greater epithelization, reduced inflammation, and increased angiogenesis, all of which sped up the healing process. Furthermore, there was a noteworthy distinction in the formation of extracellular matrix and collagen fiber synthesis between the experimental and control groups. Conclusion: Topical treatment using ready herbal extracts of olive oil and cinnamon was more successful in facilitating the recovery of traumatic ulcers.

Testing a new micro-osteoperforation modality on the rate of orthodontic tooth movement in a rabbit model

Objectives. The current study objective was to test and compare the impact of a new micro-osteoperforation (MOP) modality with 2 other modalities on the rate of tooth movement (RTM) in rabbits. Methods. The sample comprises 45 adult male albino rabbits aged 24 weeks and divided equally into 3 groups regarding MOP modalities; a single vertical MOP (1V-MOP), a single vertical MOP + 2 horizontal MOPs (1V+2H-MOP), as the new MOP design; and 3 horizontal MOPs (3H-MOP). The first right mandibular premolar (RMP1) received MOP(s) + orthodontic force 50g by traction spring. The contralateral tooth on left (LMP1), as positive (+ve) control, received only 50g orthodontic force by another spring. Both springs were fixed by a mini-screw between the 2 lower incisors. Evaluations of the clinical RTM and histopathology with H and E stain of the compressed PDL at the cervical, middle, and apical thirds of MP1 root were performed for comparing after 1, 2, and 3 weeks of OTM respectively. Results. The RTM (P&lt;0.001) and number of osteoclasts, osteoblasts, blood vessels and PDL width (P&lt;0.001 to P=0.039) significantly increased in the MOP groups; the 1V+2H-MOP was in between the higher 1V-MO P and the lower 3H-MOP. Conclusion. All MOP modalities could efficiently accelerate RTM and increase the activity of the compressed PDL, but with a variant rate. The new 1V+2H-MOP modality can be chosen as the best modality for faster RTM, because it showed moderate RTM after the 1V-MOP.

INFLUENCE OF VITAMIN E ON PANCREATIC ACINAR CELL MORPHOLOGY AND SERUM AMYLASE CONCENTRATIONS IN ALCOHOL-INDUCED PANCREATIC TOXICITY.

Misusing alcohol can cause damage to different tissues in the body, resulting in conditions like alcoholic liver disease, pancreatitis, cardiomyopathy, neurotoxicity, muscle wasting, weakened immune system, hormonal disruptions, birth defects, and bone loss. The objective of this research was to evaluate how alcohol affects the exocrine pancreas histology and the levels of amylase in the blood serum. Additionally, it aimed to explore whether vitamin E provides a safeguard against alcohol-induced harm to the pancreas in rabbits. A laboratory-based experimental investigation was carried out at Peshawar Medical College involving eighteen healthy adult male domestic rabbits weighing between one to one and a half kilograms each. The rabbits were divided into three groups. Group A, serving as the control, received normal saline as a placebo. Group B was administered a daily dose of 30 percent ethanol solution (30 ml/kg/day) in normal saline. Group C received a daily oral dose of 30% ethanol solution (30 ml/kg/day) in normal saline along with vitamin E (50 mg/kg/day). Blood samples were collected for serum amylase analysis, while morphological assessment of acinar cells involved evaluating cell count, acinar size, acinar cell size, and acinar nucleus size. Serum amylase levels did not exhibit a statistically significant variance between the control and experimental groups as p-value was >0.05. Furthermore, no notable distinctions were noted in the size and number of pancreas acini, cells of pancreatic acini, and pancreatic acinar cells nuclei between the control and experimental groups in both category E4 and Category E8, as p >0.05. There were no significant variations noted in the size and number of acini in pancreas, cells in pancreatic acini, and nuclei of cells in pancreatic acini. Consequently, the protective role of vitamin E against alcohol-induced pancreatic damage was not conclusively identified.

The protective effects of L-Carnitine against oxidative toxicity in adult male New Zealand Rabbits

The protective effects of L-Carnitine against oxidative toxicity in adult male New Zealand Rabbits

Diffusion- and Perfusion-Weighted Imaging to Detect Neurological Deficits in Acute Focal Cerebral Ischemia in Rabbits.

To investigate the relationship of diffusion-weighted imaging (DWI) and perfusion-weighted imaging (PWI) parameters with dysfunction in acute focal cerebral ischemia (ACI) rabbits. The model of ACI in the middle cerebral artery was made using 30 adult male New Zealand rabbits. The dysfunction severities of the ACI rabbits were assessed using Purdy's score. A paired-sample rank sum test was adopted to compare the abnormal signal zone (ASZ) volumes from T2 weighted imaging (T2WI), dynamic susceptibility contrast-enhanced (DSC) imaging, and DWI with a relative cerebral blood flow (rCBF) map; correlations were analyzed between the volume of each ASZ and Purdy's score by Spearman's rank correlation coefficient. The degree of necrotic and apoptotic cells was evaluated in the ASZ from DWI and DSC PWI-DWI mismatch (PDM) zone. Correlations were analyzed between the index of cellular damage and Purdy's score, the volume of ASZs by Spearman's rank correlation coefficient. The ASZ volumes from DSC-PWI and the rCBF maps were larger than those from DWI (p < 0.001 and p < 0.001, respectively); those from the rCBF map (Z = 0.959, p < 0.001) and DSC-PWI (Z = 0.970, p < 0.001) were positively correlated with DWI; a positive correlation was found between Purdy's score and the ASZ volumes from DSC-PWI (Z = 0.889, p < 0.001), DWI (Z = 0.921, p < 0.001), and rCBF (Z = 0.891, p < 0.001). A significant difference was observed between the ASZ from DWI and the PDM zone in terms of the degree of necrotic (p < 0.001) and apoptotic cells (p < 0.001). The degree of cellular damage in the ASZ of DWI and PDM zone had no relationship with Purdy's score and the volumes of ASZs. The ASZ volumes from DSC-PWI, rCBF, and particularly DWI reflected the level of dysfunction in rabbits with ACI.

Impact of Freeze-dried Corticocancellous Bone Allograft Combined with Enamel Matrix Derivative in the Treatment of Critical-sized Calvarial Bone Defects: An Animal Study.

This study compared the quality and quantity of newly formed bone in rabbits' critical-sized calvarial defects filled with enamel matrix derivative (EMD) combined with freeze-dried bone allograft (FDBA) vs FDBA alone. A total of 24 adult male white New Zealand rabbits were included. In each rabbit, three bone defects with a diameter of 8 mm were created on the calvarium bone; the first defect was left untreated, while the second was filled with FDBA, and the third was filled with EMD + FDBA. Twelve rabbits were randomly euthanized after a month, and the remaining 2 month postsurgery. Bone sections were histologically evaluated by hematoxylin and eosin and vascular endothelial growth factor (VEGF), alkaline phosphatase (ALP), osteoprotegerin (OPG), and receptor activator of NF-kappaB (RANK) immune-histochemical staining. An improvement in the newly formed bone percentage was found in the defects filled with EMD + FDBA in comparison with FDBA and control defects at 1 month and 2 months postsurgery. Additionally, the expression of VEGF, ALP, OPG, and RANK showed highly significant differences in the defects filled with EMD + FDBA compared to the FDBA and control ones at 1 month postsurgery (p = 0.001). Meanwhile, VEGF and ALP expression showed a significant decrease in defects filled with EMD + FDBA compared to the FDBA and control ones (p = 0.001), while OPG and RANK expression showed non-significant differences between treated groups at 2 months postsurgery. Enamel matrix derivative combined with FDBA has a synergistic effect on bone formation and graft substitution. This combination accelerates the expression of VEGF, ALP, OPG, and RANK. The combination of EMD and FDBA accelerates and ameliorates the quality of newly formed bone, aiding in maxillofacial reconstruction. How to cite this article: Zakri RN, Grawish ME, Mowafey B, et al. Impact of Freeze-dried Corticocancellous Bone Allograft Combined with Enamel Matrix Derivative in the Treatment of Critical-sized Calvarial Bone Defects: An Animal Study. J Contemp Dent Pract 2024;25(5):424-431.

Integrating myocardial metabolic imaging and stress myocardial contrast echocardiography to improve the diagnosis of coronary microvascular diseases in rabbits.

Persistent challenges associated with misdiagnosis and underdiagnosis of coronary microvascular disease (CMVD) necessitate the exploration of noninvasive imaging techniques to enhance diagnostic accuracy. Therefore, we aimed to integrate multimodal imaging approaches to achieve a higher diagnostic rate for CMVD using high-quality myocardial metabolism imaging (MMI) and myocardial contrast echocardiography (MCE). This combination diagnostic strategy may help address the urgent need for improved CMVD diagnosis. In this study, we established five distinct pretreatment groups, each consisting of nine male rabbit: a fasted group, a nonfasted group, a sugar load group, an acipimox group, and a combination group of nonfasted rabbits administered insulin. Moreover, positron emission tomography-computed tomography (PET/CT) scan windows were established at 30-, 60-, and 90-minute intervals. We developed 10 CMVD models and conducted a diagnosis of CMVD through an integrated analysis of MMI and MCE, including image acquisition and processing. For each heart segment, we calculated the standardized uptake value (SUV) based on body weight (SUVbw), as well as certain ratios of SUV including SUV of the heart (SUVheart) to that of the liver (SUVliver) and SUVheart to SUV of the lung (SUVlung). Additionally, we obtained three coronary SUVbw uptake values. To clarify the relationship between SUVbw uptake values and echocardiographic parameters of the myocardial contrast agent more thoroughly, we conducted a comprehensive analysis across different pretreatment protocols. Receiver operating characteristic (ROC) curve analysis was employed to evaluate the diagnostic accuracy of each parameter in the context of CMVD. In the context of MMI, the nonfasted-plus-insulin group, as observed during the 60-minute examination, exhibited a noteworthy total 18F-fluorodeoxyglucose (18F-FDG) uptake of 47.44±6.53 g/mL, which was found to be statistically different from the other groups. To ascertain the reliability of the results, two double-blind investigators independently assessed the data and achieved a good level of agreement, according to the intraclass correlation coefficient (ICC) (0.957). The SUVbw of the nonfasted-plus-insulin group exhibited a moderate correlation with the microvascular blood flow reserve (MBFR) parameters derived from the MCE examination, as evidenced by a r value of 0.686. For the diagnosis of CMVD disease, the diagnostic accuracy of the combined diagnostic method [area under the curve (AUC) =0.789; 95% confidence interval (CI): 0.705-0.873] was significantly higher than that of the MBFR (AUC =0.697; 95% CI: 0.597-0.797) and SUVbw (AUC =0.715; 95% CI: 0.622-0.807) methods (P<0.05). Our study demonstrated the feasibility of a simple premedication approach involving free feeding and intravenous insulin in producing high-quality gated heart 18F-FDG PET/CT images in adult male New Zealand white rabbits. This technique holds considerable potential for ischemic heart disease research in rabbits and can enhance CMVD diagnosis via the comprehensive assessment of myocardial metabolism and perfusion.

Osteoproductivity of Injectable Bone Grafts with and without Ostrich Eggshell Membrane Protein in Rabbit Femur.

The aim of this study was to evaluate the biocompatibility and effectiveness in terms of osseointegration of dental implants composed of novel injectable bone grafts with and without ostrich eggshell particles and membrane protein in rabbit femur. Sixteen adult male New Zealand rabbits were used in this study. A bone defect was created in each animal's right and left femur, and a dental implant was placed adjacent to the defect. Two graft materials were prepared, one containing the membrane protein and the other not. In two groups, the defects were filled with these materials. In the negative control group (NC, (n:8)), the defects were left empty. A commercial product of biphasic calcium sulfate was used as a positive control material (PC, n = 8). The graft groups were defined as the group with the membrane protein (MP+, (n:8)), and without the membrane protein (MP-, n:8). The animals were euthanized at the 12th week after surgery. The samples were investigated using histology, histomorphometry, and micro-computed tomography. Data were statistically analyzed using one-way ANOVA and Tukey's tests (p = 0.05). Both the PC and MP+ groups had similar newly formed bone areas, and the mean values of these groups were significantly (p < 0.05) higher than those of the MP- and NC groups. The PC group had the highest amount of unresorbed material, while the MP- group had the lowest amount of unresorbed material. The bone-implant contact (BIC) scores of the PC and MP+ groups were significantly higher (p < 0.05) than that of the NC group. The connective tissue area of the PC group was the lowest, which was significantly lower than the other groups (p < 0.05). The grafts produced are highly biocompatible and also showed osteoproductivity. Their cost-effectiveness and osteoproductive activity require further investigation.

In vivo and in vitro efficacy of the ithmid kohl/zinc-oxide nanoparticles, ithmid kohl/Aloe vera, and zinc-oxide nanoparticles/Aloe vera for the treatment of bacterial endophthalmitis

The aim of this study was to investigate the efficacy of the ithmid kohl/zinc-oxide nanoparticles (ZnONPs), ithmid kohl/Aloe vera, and ZnONPs/Aloe vera in the treatment of bacterial endophthalmitis. The endophthalmitis model was prepared by contaminating both eyes of 24 healthy adult male albino rabbits with a clinical isolate of Klebsiella pneumoniae. The animals were randomly divided into eight groups (A-H) according to the treatment. Group A received 1 ml of ithmid kohl/ZnONPs ointment, group B received 1 ml of ithmid kohl/Aloe vera gel ointment, group C received 1 ml of ZnONPs/Aloe vera gel ointment, and groups D, E, and F were treated with 1 ml of ithmid kohl solution (0.5 g/ml in distilled water), 1 ml of ZnONPs (0.5 g/ml) colloidal dispersion, and 1 ml of Aloe vera gel, respectively. Group G received 100 μl of a tetracycline antibiotic solution (final concentration: 16 µg/ml), and group H received sterile distilled water (no treatment). In vitro antibacterial activity was evaluated against K. pneumoniae using the agar well diffusion. The combination of ithmid kohl/ZnONPs was the most effective formulation for treating endophthalmitis model in infected rabbits within 2 days. In vitro antibacterial assay confirmed the potential of the ithmid kohl/ZnONPs formulation, which had the largest zone of inhibition (31 mm) among the compounds tested. The preparation of the ithmid kohl/ZnONPs formulation and its in vivo experiment in albino rabbits for the treatment of bacterial endophthalmitis was an innovative approach that has shown promise and may potentially serve as a viable alternative in clinical practice.

Effect of Mesenchymal Stem Cells-Derived Exosomes on Healing of a Rabbit Model of Corneal Alkali Burn. Histological and Immunohistochemical Study

Abstract Introduction Corneal alkali burn is one of the most severe ophthalmic emergencies that requires prompt treatment. Recently, the attention of researchers has been attracted to the use of exosomes as a novel non-cell therapy for treatment of corneal alkali burn. Aim to evaluate the possible role of mesenchymal stem cells (MSCs)-derived exosomes on healing of experimentally induced corneal alkali burn in rabbits by histological and immunohistochemical techniques. Materials and Methods Forty-five adult male New Zealand white rabbits were divided into three groups: group I (control group); group II in which corneal alkali burn was left for spontaneous healing, and group III (exosomes treated group): in which rabbits received a sub-conjunctival injection of 100 μg MSCs-exosomes after one hour from corneal alkali burn induction. Injection of exosomes was repeated every other day. All groups were further subdivided into two subgroups; subgroup A and B, where corneal specimens were collected after 7 days and 14 days, respectively. Characterization of exosomes was done using transmission electron microscope. Gross examination of the cornea was done at days 0, 1, 4,7,10 and 14. At the end of experiment, corneal specimens were collected and subjected to hematoxylin and eosin, Masson`s trichrome, periodic acid Schiff, transforming growth factor-beta and vascular endothelial growth factor immunohistochemical techniques. Histo-morphometric study and statistical analysis were also done. Results Administration of MSCs-derived exosomes in group III improved healing of corneal alkali burn by inhibiting angiogenesis and inflammation, enhancing corneal reepithelization, and providing better organization of newly formed stromal collagen fibers. Conclusion Sub-conjunctival injection of MSCs-derived exosomes could be effective in the healing of corneal alkali burn in adult rabbits.

Influence of Alcoholic Garlic Extract on Wound Healing of Male New Zealand White Rabbits Full Thickness cutaneous Injuries

Garlic can be used to effectively heal wounds due to its high levels of antioxidants and antimicrobials. This study examined the effects of garlic extract on rabbit models of wound healing. using fresh garlic, extract was produced. The experiment involved sixteen adult male New Zealand white rabbits, weighing between 1±0 and 300 kg, and ranging in age from 9 to 12 weeks. The animals were split up into two major groups at random, with eight animals in each group. Group (A) the skin wounds were excluded from treatment as the control group. In Group (B), the wound was treated by application of 15% garlic extract for only 2 times per day at first 7 consecutive days immediately post wounding . Macroscopic results have shown significant decrease of the wound size (P ≤ 0.05) after 7 and 14 days, in comparison with the control group in the treated groups .The epithelization period in treated groups were seen at (7) days which was shorter than that of control group (10) days, and the control group have reveald less contraction( P ≤ 0.05) than that of other treatment groups. microscopic examinations results of group A (control) at 7th days PW. appeared infection, congestion, delayed re-epithelization of the wound, severe inflammatory cell infiltration , while at 14th days PW. ,there were abscessation with thick crust, cracked and splintered scab , severe hemorrhage beneath the epidermis, thick and coarse collagen fibers, a little quantity of partial stratum basal hyperplasia and granulation tissue. At With 14 days PW.There were thin crust above the wound which appeard partially detached from epidermis, profuse granulation tissue , complete and thin epidermal layer, marked stratum basal hyperplasia , meeting of wound edges with absence of hemorrhage beneath the epidersmis , formation of new and small haire follicles, the epidermis and thin, regular layer of collagen are covered in neither a crust nor a fully keratinized layer fibers start to develop.

Effects of moringa oleifera leaves powder on lipid parameters of adult male rabbits.

Effects of moringa oleifera leaves powder on lipid parameters of adult male rabbits.

Investigation Of Lectin Binding On Rabbit Spleen Cell Membrane Infected With Proteus vulgaris

This study investigated the effects of Proteus vulgaris OX19 infection on the carbohydrate composition of spleen cell membranes in New Zealand adult male rabbits. Rabbits were injected with increasing doses of P. vulgaris OX19 (0.5 ml, 1 ml, 2 ml, 4 ml, 5 ml) at five-day intervals over the course of one month. Following the treatment period, spleen tissues were collected from both the control and infected groups. Tissue sections were stained using the Avidin-Biotin-Peroxidase method with five different lectins: Concavalia ensiformis (Con A), Arachis hypogaea agglutinin (PNA), Bauhinia purpurea agglutinin (BPA), Griffonia simplicifolia I (GS-I), and Ulex europaeus agglutinin I (UEA-I). The stained sections were examined by light microscopy to evaluate lectin binding. Among the lectins used, Con A showed strong binding (+++) to spleen cell membranes of the Proteus-infected group, while moderate binding (++) was observed in the control group. UEA-I exhibited weak binding in the control group but demonstrated moderate binding in the Proteus-infected group. In contrast, PNA, BPA, and GS-I exhibited strong binding (+++) to spleen cell membranes in the control group and moderate binding (++) in the infected group. These findings suggest that P. vulgaris OX19 infection induces alterations in the carbohydrate moieties of glycoproteins and glycolipids in the spleen cell membranes of infected rabbits. It is hypothesized that P. vulgaris modifies the terminal carbohydrates of glycoproteins and/or glycolipids in spleen cell membranes, contributing to the observed changes in lectin binding patterns.