Abstract To asses the level of awareness and knowledge of Human papillomavirus (HPV), cervical cancer (CC) and Human papilomavirus vaccine (HPVV) among adolescents in senior secondary school in Ibadan North, Nigeria. This cross-sectional descriptive study carried out among 160 female and male adolescents in two private and two public senior secondary school of Ibadan North Local Government. A semi-structured questionnaire which included questions relating to level of awareness and knowledge of Human papillomavirus infection, cervical cancer and human papillomavirus vaccine was used to obtain information from the respondents. Data were analyzed using descriptive statistics and chi-square test (p<0.05). Respondents' mean age was 13.0±1.7 year and more than half of the respondents were female (51.9%), majority of the respondents were in grade 10 (39.4%) and grade 12(33.8%) respectively, 87.3% of the respondents live with both parents. Sixty-eight percent of the respondents have heard of HPV, the main sources of information were from school (41.2%),mass media (27.5%) and seminar attended (13.7%) respectively,32% of the respondents knew that HPV is a sexually transmitted infection,31.8% acknowledge it as viral infection and 35.8% agreed that it can infect both male and female,41.9% agreed that it transmitted through sharing of toiletries and sharp objects,30% were aware that it can be transmitted through sexual intercourse while 28% of the respondents believed that early age of sexual debut is a risk factor and having multiple sexual partners predisposes to HPV infection while 29.4% of the respondents agreed that engaging in an unprotected sex is a risk factor for transmitting HPV infection. However, 41.3% of the respondents who were aware and knowledgeable that abstinence is a good measure for preventing HPV infection. Only 15.6% have heard of HPVV, 18.8% were aware that it can prevent it and HPVV is meant for both male and female. Living with parent and being in either private or public school was not statistical significant with the knowledge of HPV (p>0.70), categories of classes of the respondent was not significantly associated with their knowledge of HPV vaccine (p>0.63), there is significant relationship between the gender and awareness that having multiple sexual partner predisposes to HPV (p<0.009). Level of awareness and knowledge of HPV infection, cervical cancer and HPV vaccine were very low among the adolescents, programmers that promote knowledge should be incorporated into the curriculum, teachers of various school be updated. Keywords: Human papillomavirus infection, adolescents, HPV vaccine, knowledge Citation Format: OLUBUNMI OLABISI ADETULE. AWARENESS AND KNOWLEDGE OF HUMANPAPILLOMAVIRUS AND HPV VACCINE AMONG ADOLESCENTS IN NORTH IBADAN, NIGERIA: A PREVENTIVE TOOL FOR FUTURE LEADERS. [abstract]. In: Proceedings of the Ninth AACR Conference on the Science of Cancer Health Disparities in Racial/Ethnic Minorities and the Medically Underserved; 2016 Sep 25-28; Fort Lauderdale, FL. Philadelphia (PA): AACR; Cancer Epidemiol Biomarkers Prev 2017;26(2 Suppl):Abstract nr A60.