ABSTRACT Background Reductions in the age of onset of smoking in adolescence is a public health concern. Evidence suggests that infrastructural factors play an important role in adolescent smoking behavior. Therefore, the present study aimed to perform a psychometric analysis of the questionnaire of infrastructural factors on smoking behavior and evaluate the theoretical framework with a path analysis approach in adolescents in Isfahan, Iran. Methods A total of 340 adolescents in Isfahan, Iran selected using simple random stratified cluster sampling were included in the present psychometric cross-sectional research. The data collection tool was a researcher-made questionnaire of infrastructural factors. Results The participants’ mean age was16.67(0.98) years. The total corrected item-total correlations index was 0.714. Considering the three-factor assumption of the present study and identification of two factors, confirmatory factor analysis (CFA) was performed simultaneously in both models. The results of path analysis showed acceptable goodness of fit for the explanatory model of infrastructural factors. Conclusions This study proposed a reliable and valid explanatory model of infrastructural factors and its questionnaire for smoking behavior. The questionnaire can also be utilized in future research on smoking behavior in adolescents.