Sixty Simmental crossbred heifers 18 to 20 mo of age were detected in estrus and assigned at random to a 2 x 2 factorial design study with 30 controls, and 30 given FSH. Half of each group was given prostaglandin F2 alpha (PGF) i.v. and the rest i.m. Injections of follicle-stimulating hormone were started on d 7 to 14 of an estrous cycle and continued for 5 d or until ovariectomy; PGF was administered either i.v. or i.m. at 48 (25 mg) and 60 (15 mg) h after the initial FSH injection. Control females received a similar PGF treatment on a day between d 9 and 15 of the estrous cycle. Blood samples were collected from all animals immediately before PGF administration and every 12 h thereafter until ovariectomy. Within each of the four experimental subgroups, ovariectomies were performed at either 24, 48 or 72 h (five/time group) after initial PGF injection. Ovarian and corpus luteum (CL) weights were recorded as well as number and size of follicles and number of ovulations. Regression of the CL was slower (P less than .05) after administration of PGF i.v. than i.m. (CL weight was 2.6 vs 3.3 +/- .2 g for i.m. and i.v. groups, respectively). Exogenous FSH increased estradiol-17 beta (E2) concentrations, and FSH-treated heifers had more (P less than .05) early ovulations than control heifers did. Ovulations in FSH-treated heifers had begun to occur by 24 h after i.v. and i.m. PGF injection.(ABSTRACT TRUNCATED AT 250 WORDS)