Bone morphogenetic protein (BMP) signaling pathway has been known to be involved in the regeneration of kidney lesions. Although the administration of BMPs could be used to improve the repair of the injured renal tissue, the studies indicated that the activation of signaling pathways in the mesenchymal stem cells (MSCs) could be more effective in the improvement of tissue damage. The aim of the present study was to investigate whether the HEK293 conditioned medium (HEK293-CM) treatment improved the expression of BMP signaling pathway genes in the human adipose mesenchymal stem cells (AD-MSCs). The AD-MSCs were cultured in the medium including different concentrations (5%, 10% and 15%) of HEK293-CM for three days. After RNA extraction and cDNA synthesis, the expression level of BMP2, BMP6 and BMP11 were determined in the treated cells by SYBR Green Real time PCR. The evaluation of the human AD-MSCs treated by HEK293-CM revealed that 5% CM prepared from the 24-hour exposure of HEK293 to the medium supplemented with 5% of FBS (5%CM-5%FBS-24 h) increased the expression of BMP6 and BMP11 in the AD-MSCs. Furthermore, the results obtained from the Real time PCR analysis showed that the concentration and incubation time to prepare the conditioned medium were the important factors in the activation of BMP signaling pathway in the AD-MSCs through treatment with HEK293-CM.