Administration Of Aloe Vera Gel Research Articles

Molecular Docking and Efficacy of Aloe vera Gel Based on Chitosan Nanoparticles against Helicobacter pylori and Its Antioxidant and Anti-Inflammatory Activities.

The medicinal administration of Aloe vera gel has become promising in pharmaceutical and cosmetic applications particularly with the development of the nanotechnology concept. Nowadays, effective H. pylori treatment is a global problem; therefore, the development of natural products with nanopolymers such as chitosan nanoparticles (CSNPs) could represent a novel strategy for the treatment of gastric infection of H. pylori. HPLC analysis of A. vera gel indicated the presence of chlorogenic acid as the main constituent (1637.09 µg/mL) with other compounds pyrocatechol (1637.09 µg/mL), catechin (1552.92 µg/mL), naringenin (528.78 µg/mL), rutin (194.39 µg/mL), quercetin (295.25 µg/mL), and cinnamic acid (37.50 µg/mL). CSNPs and A. vera gel incorporated with CSNPs were examined via TEM, indicating mean sizes of 83.46 nm and 36.54 nm, respectively. FTIR spectra showed various and different functional groups in CSNPs, A. vera gel, and A. vera gel incorporated with CSNPs. Two strains of H. pylori were inhibited using A. vera gel with inhibition zones of 16 and 16.5 mm, while A. vera gel incorporated with CSNPs exhibited the highest inhibition zones of 28 and 30 nm with resistant and sensitive strains, respectively. The minimal inhibitory concentration (MIC) was 15.62 and 3.9 µg/mL, while the minimal bactericidal concentration (MBC) was 15.60 and 7.8 µg/mL with MBC/MIC 1 and 2 indexes using A. vera gel and A. vera gel incorporated with CSNPs, respectively, against the resistance strain. DPPH Scavenging (%) of the antioxidant activity exhibited an IC50 of 138.82 μg/mL using A.vera gel extract, and 81.7 μg/mL when A.vera gel was incorporated with CSNPs. A.vera gel incorporated with CSNPs enhanced the hemolysis inhibition (%) compared to using A.vera gel alone. Molecular docking studies through the interaction of chlorogenic acid and pyrocatechol as the main components of A. vera gel and CSNPs with the crystal structure of the H. pylori (4HI0) protein supported the results of anti-H. pylori activity.

The possible ameliorating role of Aloe vera gel on the toxic Effect of Acrylamide on the jejunum of Female albino rats (Light and Scanning Electron Microscopic study)

ABSTRACTBackground: Acrylamide, an industrial chemical found in many industrial applications as well as for chemical and environmental purposes. The Aloe vera plant Known for many years and was known and used for its health, beauty, medicinal and skin care properties. The name Aloe vera derives from the Arabic word “Alloeh” meaning “shining bitter substance,” while “vera” in Latin means “true.” 2000 years ago.Objective: The aim of the study was to evaluate the role of the Aloe vera extract and its influence on experimental lesions in the jujenal mucosa caused by Acrylamide.Materials and Method: Thirty adult female albino rats were used and divided equally into three groups (10 rat/eachgroup): (I)control group. (II)Acrylamide treated group (5 mg/kg/day). (III) AcrylamideAirregular mucosal folds; cellular infiltration and thickened discontinued muscularis externa, with electron microscopic study there were areas of erosion and ulceration of the villi with loss of the normal picture . Administration of Aloe vera gel restored the normal architecture of the villi and crypts with normal appearance of most layers, scanning electron microscopy showed intact epithelium withgoblet cells openings discharging mucus.Conclusion:Acrylamide markedly affect the jujenal mucosa as seen by light and electron microscope, Aloe vera gel administration can protect the mucosa from this harmful effect.Keywords: Acrylamide, Aloe vera, jejunum, Rats.

Protective effects of aloe vera gel (aloe baberdensis Miller) on aluminum chloride-induced reproductive toxicity in male Wistar rats.

Objective:This study aims at investigating the protective effects of aloe vera gel on aluminum chloride-induced testicular toxicity of adult Wistar rats.Methods:Twenty male Wistar rats were randomly divided into four groups (A, B, C, and D) with five animals per group. Group A serves as the control group and received distilled water (1ml/Kg). Group B received distilled water (1ml/Kg) with 100mg/kg b.wt of aluminum chloride daily. Group C received 100mg/kg b.wt of aluminum chloride with 600mg/kg b.wt of Aloe vera gel daily. Group D received 100mg/kg b.wt of aluminum chloride with 5mg/kg b.wt of vitamin C daily. The animals were fed on standard laboratory animal diet and water ad libitum. Administration was via oral cannula for four weeks. The rats were slaughtered using cervical dislocation. The testes were harvested for seminal, biochemical and histological analysis.Results:The results demonstrate that the administration of aloe vera gel (AVG) is capable of preventing testicular toxicity due to aluminum chloride. Aluminum chloride caused a significant change in the testes and seminal parameters of group B when compared to the control animals. The level of Nitric oxide (NO) increased and the level of Superoxide dismutase (SOD) decreased significantly in rats treated with aluminum chloride.Conclusions:The administration of aloe vera gel showed a preventive response in aluminum-induced testicular toxicity of rats as evidenced by histological and biochemical analysis.

Clinical efficacy of an Aloe Vera gel versus a 0.12% chlorhexidine gel in preventing traumatic ulcers in patients with fixed orthodontic appliances: a double-blind randomized clinical trial.

Traumatic oral ulceration (TOU) is one of the most common side effects of orthodontic treatments. The objective of this trial is to compare the clinical efficacy of an 80% Aloe Vera gel, prepared using a master formula, versus a commercial 0.12% Chlorhexidine (CHX) gel for TOU prevention in participants wearing fixed orthodontic appliances. This report represents a single-centre, university-based, double-blinded, randomized controlled trial with 2 parallel arms. Patients aged 12years or older, in the permanent dentition, and about to start fixed orthodontic treatment in this university setting were randomly allocated to use either Aloe Vera or CHX gel, following the cementation procedure. Pre-treatment and 1 month after the cementation clinical assessment and digital photographic images were taken of the teeth and assessed by 2 clinical assessors for the presence or absence of TOUs. A total of 140 were randomized and completed the trial. The overall prevalence of TOUs was 43.6%. Overall 5.7% of patients treated with Aloe Vera gel showed did not suffer from TOUs, whereas in the CHX arm, a total of 57 (81.4%) were affected by this outcome reaching a significant result (p < 0.001). In terms of relative risk (RRs) and confidence intervals (CIs), Aloe Vera provided better results than CHX with an RR of 0.07 (95%CI 0.03-0.16; p < 0.001), and with a patients' number needed to treat of 1.3 (95%CI 1.16-1.54). There were no adverse effects. These results suggest that Aloe Vera gel administration in patients with fixed orthodontic appliances could be important for effective prevention of TOU.

PERAN GEL LIDAH BUAYA DALAM MENGURANGI XEROSIS

The management of patients with Chronic Renal Failure (CRF) is theimmediate treatment of the infection to prevent infection to the patient's kidney due todecreased immunity. CRF patients almost all have dermatological disorders, includingxerosis. Pharmacological treatment causes a lot of side effects. One natural ingredient isaloe vera. Aloe vera as a moisturizer, antibiotics and as well itching. This study aims toanalyze the effectiveness of aloe vera gel as a material alternative to nursingimplementations in xerosis CRF patients in Semarang hospitals. This type of research isexperiment with using pretest posttest control group design, selection sampling withrandom sampling taking random table. The number of respondents 36 people, that are 18people giving the control group a placebo gel skin lotion 2 times daily after bath for 3days and 18 people experimental group with the administration of aloe gel 2 times dailyafter bath for 3 days. Observations using Akhyani modifications observation sheet.Xerosis difference in the two groups were analyzed by paired samples T-test, while therelationship with the respondent characteristics xerosis is analyzed by independentsample T-test and corelations. Statistical test results showed no significant differencexerosis before and after administration of aloe vera gel with xerosis value (p =0.000).The implication of this study is the Aloe vera gel can be considered to bealternatives to nursing implementations xerosis patients with CRF.

Beneficial effects of Aloe vera gel on lipid profile, lipase activities and oxidant/antioxidant status in obese rats

Natural products can be very effective in the prevention of obesity-related metabolic disorders. The aim of the present study was to investigate the anti-obesity effects of Aloe vera (Aloe barbadensis Miller) gel administration at 100 and 200 mg/kg/day, in a diet-induced obesity rat model. The whole gel was used. Plasma and tissue parameters and adipose lipase activities were determined. Our results emphasized that obese rats displayed dyslipidemia and stress oxidative, with excessive adipose tissue lipids. Aloe vera gel administration at 100 and 200 mg/kg/day prevented adipose tissue accumulation and corrected the dyslipidemia and oxidative stress. In addition, it induced adipose LPL inhibition and HSL activation in obese rats. In conclusion, Aloe vera reduced fat accumulation via its protective role against obesity-related metabolic alterations and antioxidant effects. Aloe vera has great potential as functional foods in the activation adipose lipolysis and the prevention of obesity-related metabolic alterations.

Aloe vera and garlic ameliorate deleterious consequences of thermoxidized palm oil diet on liver function and histology in rat

Purpose A plethora of publications have reported several cytotoxic effects associated with chronic consumption of thermoxidized palm oil. This research aims to investigate the effects of garlic and Aloe vera on liver function and hepatic cytoarchitecture in rats fed thermoxidized palm oil diet. Design/methodology/approach Thirty-five male albino Wistar rats weighing 150-180 g were used for this study. They were randomly assigned into five groups (n = 7): control, thermoxidized palm oil diet fed (TPO), TPO plus garlic juice (TPO + G), TPO plus Aloe gel (TPO + A) and TPO plus garlic/Aloe gel (TPO + G + A). The TPO diet was prepared by mixing 15 g of cooled thermoxidized palm oil with 85 g of rat feed. The juice and gel were orally administered at doses of 2.00 ml/kg and 19.12 ml/kg, respectively. After 3 months of feeding and administration, the animals were sacrificed using standard methods and blood collected via cardiac puncture for analysis. Findings Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase were significantly increased (p &lt; 0.001) in the TPO group compared to the control. This increase was reduced (p &lt; 0.001) in all the treated groups compared to TPO, with the greatest decrease (p &lt; 0.001) seen in TPO + A. Total bilirubin was increased (p &lt; 0.001) in the TPO group compared to the control, whereas there was a significant decrease (p &lt; 0.001) in all the treated groups. Serum proteins and plasma fibrinogen were lowered (p &lt; 0.001) in the TPO group compared to the control but increased progressively in all the treated groups. TPO induced prominent histopathological derangements of the liver tissues. However, there were marked improvements following treatment with garlic and Aloe vera. Originality/value The results obtained in this study have revealed that chronic consumption of thermoxidized palm oil is hazardous to health by inducing hepatotoxicity, as seen in increased ALT, AST and total and unconjugated bilirubin and decreased total protein. However, these debilitating effects were seen to be greatly ameliorated following garlic juice and Aloe vera gel administration. If these results are to be extrapolated to humans, then the chronic consumption of thermoxidized palm oil diet should be seriously discouraged.

The effect of aqueous Aloe vera gel extract on serum mineral compositions of Red Sokoto Bucks (Maradi)

This study was conducted to determine the effect of Aloe vera gel extract on the serum mineral compositions of Red Sokoto bucks. Blood samples were collected from 30 bucks before the commencement of administration of Aloe vera gel extract for serum minerals and these served as control group. The bucks were now divided into three groups A, B and C containing ten goats per group. The Aloe vera extract was administered orally at 1.0 ml/kg body weight daily at 3.0%, 4.0% and 5.0% to the group A, B, and C respectively for 14 days period. Five milliliters (5ml) of blood samples were collected from the jugulars vein of Red Sokoto bucks in the respective animals in the groups at 7 and 14 days, deposited into anticoagulant free plastic tubes and allowed to clot at room temperature within 3 hours of collection.Sera samples were analysed for potassium, sodium, calcium, Chloride, total protein, and albumin. Data were analysed using one way analysis of variance (ANOVA). The results showed that there were significant (P&lt;0.05) differences in the values of potassium, calcium, chloride, total protein and albunin in the pretreated control group and post treatment groups while there were no significant difference in sodium level (P&gt;0.05) in the bucks that were administered with Aloe vera gel extract and control group. This indicated that oral Aloe vera gel administration in Red Sokoto bucks relatively caused some changes in mineral composition which may affect some physiological functions of visceral organs in these animals.

In vivo inhibitory effect of Aloe vera gel on the ability of mouse parental splenic lymphocytes to induce cutaneous angiogenesis in recipient F1 mice.

Lymphocyte-induced angiogenesis test (LIA) is a model of local graft-versus-host (GVH) reaction, marker of the earliest events resulting from activation of donor lymphocytes after contact with host semi-allogeneic histocompatibility antigens. The effect of in vivo oral administration of Aloe vera gel for 21 days to maternal strain (Balb/c) donor mice on the ability of their splenic lymphocytes to induce cutaneous angiogenesis (LIA test) in F1 Balb/c x C3H recipients, was studied. Neovascular reaction evaluated 72 hours after cells grafting was significantly lower in F1 mice grafted with lymphocytes collected from Aloe- fed donors, than in recipients of lymphocytes collected from respective controls. This observation opens the promise of safe and ethically acceptable possibility of use of Aloe vera gel in human donors in prevention of GVHD in recipients of bone marrow grafts.

Experimental immunology Oral administration of Aloe vera gel, anti-microbial and anti-inflammatory herbal remedy, stimulates cell-mediated immunity and antibody production in a mouse model

IntroductionAloe vera (L.) Burm. f. (Aloe barbadensis Mill) Liliaceae, succulent plant native to northern Africa, is presently cultivated in many regions of the world. Traditionally, its inner part of parenchyma, which contains aloe gel, was used for the treatment of minor wounds, inflammatory skin disorders, thermal and radiation burns and to alleviate chronic osteoarthritis pain. It also possesses some antimicrobial activity. Now, aloe gel is also increasingly consumed as a dietary supplement. Some data suggest its immunomodulatory properties.The aim of the studyThe aim of the study was to evaluate the influence of orally administered aloe gel on some parameters of cellular and humoral immunity viz. mitogen-induced proliferation of splenic lymphocytes and their chemokinetic activity, and anti-sheep red blood cells (SRBC) antibody production in Balb/c mice.ResultsDaily treatment of mice for 14 and 21 days with 50 µl or 150 µl of aloe gel dose resulted in enhanced chemokinetic activity and stronger response of their splenic lymphocytes to mitogen PHA and enhancement of anti-SRBC antibody production.

Administration of Dried Aloe vera Gel Powder Reduced Body Fat Mass in Diet-Induced Obesity (DIO) Rats

The aim of the present study was to investigate the anti-obesity effects of Aloe vera gel administration in male Sprague-Dawley (SD) rats with diet-induced obesity (DIO). SD rats at 7 wk of age were fed either a standard diet (10 kcal% fat) (StdD) or high-fat (60 kcal% fat) diet (HFD) during the experimental period. Four weeks after of HFD-feeding, DIO rats (11 wk of age) were orally administered with two doses of Aloe vera gel powder (20 and 200 mg/kg/d) for 90 d. Body weights (g) and body fat (%) of HFD fed rats were significantly higher than those of StdD-fed rats. Although a modest decrease of body weight (g) was observed with the administration of dried Aloe vera gel powder, both subcutaneous and visceral fat weight (g) and body fat (%) were reduced significantly in Aloe vera gel-treated rats. Serum lipid parameters elevated by HFD were also improved by the Aloe vera gel treatment. The oxygen consumption (VO(2)), an index of energy expenditure, was decreased in HFD-fed rats compared with that in StdD-fed rats. Administration of Aloe vera gel reversed the change in VO(2) in the HFD-fed rats. These results suggest that intake of Aloe vera gel reduced body fat accumulation, in part, by stimulation of energy expenditure. Aloe vera gel might be beneficial for the prevention and improvement of diet-induced obesity.

Aloe vera gel and thyroid hormone cream may improve wound healing in Wistar rats

Therapeutic effects of various treatment options in wound healing have been one of the most controversial issues in surgical science. The present study was carried out to examine and compare the effects of Aloe vera gel, thyroid hormone cream and silver sulfadiazine cream onsutured incisions in Wistar rats. In a randomized controlled trial, thirty-six Wistar male rats, 250 to 300 g, received surgical incisions followed by topical application of Aloe vera gel, thyroid hormone cream and silver sulfadiazine 1%. To assess the efficacy of each treatment technique, a histological approach was used to evaluate the mean number of fibroblasts, macrophages, neutrophils, blood vessel sections and thickness of the regenerating epithelium and dermis on days 4, 7 and 14. Re-epithelialization and angiogenesis were significantly improved in Aloe vera gel group compared with the other treatments while thyroid hormone cream had positive effects on day 4 (P≤0.05). Topical administration of Aloe vera gel is recommended as the treatment of choice for surgical incisions.

Aloe vera: Nature′s soothing healer to periodontal disease

Background:Recent interest and advances in the field of alternative medicine has promoted the use of various herbal and natural products for multiple uses in the field of medicine. Aloe vera is one such product exhibiting multiple benefits and has gained considerable importance in clinical research. This clinical study focuses on Aloe vera and highlights its property when used as a medicament in the periodontal pocket.Materials and Methods:A total number of 15 subjects were evaluated for clinical parameters like plaque index, gingival index, probing pocket depth at baseline, followed by scaling and root planing (SRP). Test site comprised of SRP followed by intra-pocket placement of Aloe vera gel, which was compared with the control site in which only SRP was done, and clinical parameters were compared between the two sites at one month and three months from baseline.Results:Results exhibited encouraging findings in clinical parameters of the role of Aloe vera gel as a drug for local delivery.Conclusion:We conclude that subgingival administration of Aloe vera gel results in improvement of periodontal condition. Aloe vera gel can be used as a local drug delivery system in periodontal pockets.

Effect of Aloe vera Gel Administrated on Reproductive Performance of Female Rats

The research was conducted to find out the effect of Aloe vera gel administrated orally onreproductive performance of female rats. Fifteen two months old female rats were alloted to fivetreatments of per oral Aloe vera gel administration: P0 (control), P1(1 mg /g body weight), P2 (2 mg/gbody weight, P3 (3 mg/g body weight), and P4 ( 4 mg/g body weight). The gel were administrated everyday for four weeks. The body weight were measured on the same day as last treatment. The animal thenmated to proven male on 1:1 rasio. Positively mated animals were sacrificed on D-10 after mating tocollect uterus and ovary so the number of fetus, corpus luteum, and ovary weight can be determined. Thetreatments significantly decreased (P0.05) the average body weight gain. The P0 (4.1 g/head/day) wasdifferent to P1 (2.69 g/head/day), P2 (2.87 g/head/day, and P4 (2.68 g/head/day), but was not different toP3 (3.39 g/head/day). The ovarium weight were not different (P0.05) among the treatments, so did thenumber of corpus luteum (CL), the number of fetuses and the CL: fetus rasio.Keywords: Aloe vera, rats, reproductive performance .

Protective effect ofAloe veraL. gel against sulphur mustard-induced systemic toxicity and skin lesions

OBJECTIVE: Sulphur mustard (SM), chemically 2,2'-dichloro diethyl sulphide, is an incapacitating and extremely toxic chemical warfare agent, and causes serious blisters on contact with human skin. SM forms sulphonium ion in the body that alkylates DNA and several other macromolecules, and induces oxidative stress. The aim of this study was to evaluate the protective effect of Aloe vera L. gel against SM-induced systemic toxicity and skin lesions. MATERIALS AND METHODS: Aloe vera gel was given (250, 500 and 1000 mg/kg) orally to mice as three doses, one immediately after SM administration by percutaneous route, and the other two doses on the next two days. Protective index was calculated with and without Aloe vera gel treatment. Aloe vera gel was also given orally as three doses with 3 LD50 SM and the animals were sacrificed for biochemical and histological evaluation, 7 days after SM administration. In another set of experiment Aloe vera gel was liberally applied on the SM administered skin site and the animals were sacrificed after 14 days to detect its protective effect on the skin lesions induced by SM. RESULTS: The protection given by Aloe vera gel was marginal. 1000 mg/kg dose of Aloe vera gel gave a protection index of 2.8. SM significantly decreased reduced glutathione (GSH), oxidised glutathione (GSSG) and WBC count, and significantly increased malondialdehyde (MDA) level, RBC count and Hb concentration. Aloe vera gel offered protection only in the increase of MDA level by SM. Severe damage was observed in the histology of liver, spleen and skin following SM administration, and 1000 mg/kg of Aloe vera gel partially protected the lesions. However, topical application of Aloe vera gel showed better protection of the skin lesions induced by SM. CONCLUSION: The study shows that percutaneous administration of SM induces oxidative stress and oral administration of Aloe vera gel could only partially protect it. Topical application of Aloe vera gel may be beneficial for protecting the skin lesions induced by SM.