Adjacent Pieces Research Articles

"The Jigsaw Puzzle Locking Technique" to Augment the Stabilization between the Septal Cartilage and Extension Graft in End-to-End Suture Fixation.

Nasal tip stability is crucial for long-lasting results. Usage of the extension graft is one of the most reliable stabilization techniques. With a septum in the midline, the extension graft is fixed end to end. The "jig-saw puzzle technique" reinforces this end-to-end fixation. The specific protrusion on one piece of the puzzle is inserted in a groove on adjacent piece. This settlement provides stabilization between the pieces. Application of this philosophy between the nasal septum and extension graft may provide a long-term fixation. Between April 2022 and March 2024, the "jig-saw puzzle technique" was applied in 26 female patients. Trapezoid-shaped protrusion was created at the septum. Similar indentation was created at the extension grafts. The protrusion is then settled in the indentation. The preop, immediate postoperative and one-year postoperative pictures are compared to assess the rotations and projections with Adobe Photoshop Program. All 26 patients were females with mean age 27.5 years (20-32). Satisfactory graft stabilization was obtained without loss of projection and rotation in all patients. Statistically significant difference was found between the preoperative, immediate postoperative and postoperative 1-year projection and rotation assessments of the patients (p=0.001; p<0.01). The results were evaluated at a 95% confidence interval and significance was evaluated at p<0.05 level. These statistical analyses verify that both projection and rotation were preserved with jig-saw puzzle technique. The jigsaw puzzle technique may provide suture-independent, long-term end-to-end fixation opportunity of the septal cartilage and the extension graft. This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 .

#2834 Analysis of cyst hetero- and homogeneity to unravel common pathways in autosomal dominant polycystic kidney disease

Abstract Background and Aims Autosomal dominant polycystic kidney disease (ADPKD) is the most common inherited kidney disease, with a prevalence of 1 in ca. 1000 individuals and accounting for 10% of the European dialysis population. It is most frequently (75-80%) caused by mutations in the PKD1 gene encoding Polycystin-1. Besides systemic manifestations such as liver cysts, hypertension and cardiac abnormalities, the most prominent phenotype is the gradual development of kidney cysts, leading to kidney enlargement and a decline in kidney function leading to end-stage kidney disease at an average age of ca. 50 years. The cysts arise from tubular epithelial cells in the nephron that start to dedifferentiate, proliferate and eventually secrete fluid into the cyst lumen promoting its swelling. This process is molecularly regulated by changes in Ca2+, cAMP, PKA and PI3K-Akt-mTOR signaling, among others. The renal cysts are further characterized by ciliary dysfunction, altered signal transduction, loss of polarity, apoptosis, mitochondrial abnormalities, metabolic reprogramming, inflammation, oxidative stress and epigenetic changes. However, the exact sequence of events and their relative importance in the process of cyst formation remains unclear. Therefore, we analyzed the transcriptomic cyst hetero- and homogeneity to identify the pathways that were different, but also shared among individual cysts. Method We performed RNA-Seq analysis on 40 individual cystic membranes of nephrectomized kidneys of 4 PKD1 patients. We included 4 samples of macroscopically healthy tissue from 2 ADPKD kidneys (histologically confirmed to have microcysts) and 4 samples from 4 healthy kidneys. Principal component, cluster and trajectory analysis was performed to explore cyst heterogeneity. Adjacent pieces of cystic membrane were analyzed for histology (H&amp;E, Sirius Red) or stained with FITC-tagged Lotus Tetragonolobus Lectin (LTL) or Dolichos Biflorus Agglutinin (DBA), a proximal and distal tubular marker, respectively. Results Although the cysts clearly separated from the healthy tissues in the analyses, there was also a distinct heterogeneity observed among the cysts that was independent of patient, gender, cyst size, location or fluid color. Pathway analysis revealed that these changes were characterized by a gradual increase of interstitial (remodeling) and inflammation markers, and a gradual decrease in differentiation markers (e.g. development, intercellular junctions, basement membrane) along the trajectory pseudotime. We could identify 6 clusters of cystic samples, with 2 clusters deviating from the main trajectory. With respect to tubular/epithelial dedifferentiation, the decrease in proximal tubular markers already occurred in microcystic tissue and was stronger compared to the other tubular segments. Consequently, none of the analyzed cystic membranes stained positive for LTL, while others showed clear staining for DBA. Microcysts on the other hand stained positive for LTL or DBA. Several cysts showed RNA marker expression of multiple tubular segments. Indeed, smaller macrocysts (diameter in the mm range) were lined by both LTL- and DBA-positive regions. Conclusion By analyzing transcriptomic hetero- and homogeneity among individual cysts, the molecular alterations in ADPKD progression can be tracked down and determined whether they occur similarly in all cysts or at different levels. Our data also suggest that loss of proximal tubular markers in macrocysts is more prominent compared to the decrease in distal markers. Finally, inner cyst walls can consist of cyst-lining cells of multiple origins.

Fast Recurrent Neural Network with Bi-LSTM for Handwritten Tamil Text Segmentation in NLP

Tamil text segmentation is a long-standing test in language comprehension that entails separating a record into adjacent pieces based on its semantic design. Each segment is important in its own way. The segments are organised according to the purpose of the content examination as text groups, sentences, phrases, words, characters or any other data unit. That process has been portioned using rapid tangled neural organisation in this research, which presents content segmentation methods based on deep learning in natural language processing (NLP). This study proposes a bidirectional long short-term memory (Bi-LSTM) neural network prototype in which fast recurrent neural networks (FRNNs) are used to learn Tamil text group embedding and phrases are fragmented using text-oriented data. As a result, this prototype is capable of handling variable measured setting data and gives a vast new dataset for naturally segmenting text in Tamil. In addition, we develop a segmentation prototype and show how well it sums up to unnoticeable regular content using this dataset as a base. With Bi-LSTM, the segmentation precision of FRNN is superior to that of other segmentation approaches; however, it is still inferior to that of certain other techniques. Every content is scaled to the required size in the proposed framework, which is immediately accessible for the preparation. This means, each word in a scaled Tamil text is employed to prepare neural organisation as fragmented content. The results reveal that the proposed framework produces high rates of segmentation for manually authored material that are nearly equivalent to segmentation-based plans.

TOWARDS ACCURATE INSTANCE SEGMENTATION IN LARGE-SCALE LIDAR POINT CLOUDS

Abstract. Panoptic segmentation is the combination of semantic and instance segmentation: assign the points in a 3D point cloud to semantic categories and partition them into distinct object instances. It has many obvious applications for outdoor scene understanding, from city mapping to forest management. Existing methods struggle to segment nearby instances of the same semantic category, like adjacent pieces of street furniture or neighbouring trees, which limits their usability for inventory- or management-type applications that rely on object instances. This study explores the steps of the panoptic segmentation pipeline concerned with clustering points into object instances, with the goal to alleviate that bottleneck. We find that a carefully designed clustering strategy, which leverages multiple types of learned point embeddings, significantly improves instance segmentation. Experiments on the NPM3D urban mobile mapping dataset and the FOR-instance forest dataset demonstrate the effectiveness and versatility of the proposed strategy.

TAssembly: Data-driven fractured object assembly using a linear template model

Fractured object assembly is a fundamental problem across many disciplines such as digital archaeology, accident recovery, paleontology, cranio-facial and orthopaedic surgical repair, and many more. With advances in 3D acquisition devices and improved fidelity models of fractured pieces, a flurry of semi-automatic and automatic computational assembly solutions have been developed. These methods have the potential to reduce human labor in assembling fractured objects. The challenge is to balance the time efficiency with the fidelity of the reconstructed assembly. This paper introduces TAssembly, a data-driven approach to fractured object assembly. TAssembly leverages learned feature descriptors of the underlying objects. Its assembly pipeline seamlessly integrates multiple objective terms, including feature matching, rigid matching, and regularization for matching and stitching adjacent pieces. Many experimental results show that TAssembly significantly outperforms previous fractured object assembly methods.

Piecewise Decoupling Tool Orientation Re-Scheduling for Four-Axis Reciprocal Toolpaths of Blades Based on S-θ Plane with Monotonicity Constraint

Reciprocal toolpaths with four-axis simultaneous motion of five-axis or four-axis machine tools are commonly used in the machining of blades which are widely applied in high-end equipment such as the aero-engine and the marine steam turbine. Due to the complex geometry of the blades, the tool orientation always suffers from frequent swing for this kind of toolpaths, which induces unnecessary acceleration/deceleration of the feed axes, thus degrading processing efficiency and quality. Although there are tool orientation optimization methods aiming at solving the above problem, they are mainly proposed for universal processing of the toolpaths for complex surfaces. Different from them, this paper proposes a piecewise decoupling tool orientation re-scheduling method for this kind of toolpath specifically, which takes full use of the characteristic of the reciprocal toolpaths of the blades, and takes the monotonous variation of rotation axes as an additional constraint. The re-scheduling process is realized based on the construction of a S-θ plane, where the scheduling problem is converted to the adjustment of a S-θ curve inside a feasible channel. Through two procedures, namely linearization scheduling and control-point assigning-based smoothing, the tool orientation path expressed by the S-θ curve can be effectively scheduled in a piecewise manner, and the smoothness between two adjacent pieces of the toolpaths can be ensured directly. The whole algorithm is lightweight and does not involve complex iterative operations or functional optimization solutions. Simulation and experimental tests verify the feasibility and superiority of this method. The results show that the machining efficiency of the blade is improved by 24.5%, due to the reduction of the requirement on highest feed-axis kinematics parameters after rescheduling. In addition, compared with the existing methods, the proposed method not only can improve the dynamics of feed axes in multi-axis machining, but also has advantages in computational complexity and monotonic variation property of the tool orientation.

Improving aircraft aerodynamic performance with bionic wing obtained by ice shape modulation

For Unmanned Aerial Vehicles (UAVs) with limited electrical power to achieve effectively anti-/de-icing at the leading edge of the wing, a strategy of ice shape modulation was proposed. Isolated simulated ice shape pieces printed by 3D printing technology are mounted on a NACA0012 finite wing model, and its lift/drag coefficients and suction-side velocity fields are measured by the six-component force balance and the Particle Imaging Velocimetry (PIV), respectively. The ratio of the spanwise length of a single ice shape piece to chord length and the spanwise length of the non-icing area between the two adjacent single ice shape pieces are defined as dimensionless ice shape length (w/c) and dimensionless modulation ratio (w/λ), respectively. The results indicate that for a fixed w/λ, the wing lift coefficient first increases and then drops with increasing w/c, and a peak value exists when w/c is between 0.1 and 0.2. The lower the w/λ is, the higher the wing lift coefficient will be. The periodical variation of the flow separation area along the spanwise direction is attributed on the one hand to the acceleration effect of the flow field in the non-icing area which reduces the separation area, and on the other hand to the cross-flow caused by the streamwise vortices from the non-icing area to the icing area which promotes the mixing of the flow field (similar to vortex generators). The obtained modulation law is verified through flight tests and provides guidance for the use of ice shape modulation scheme for UAVs that cannot be completely anti-/de-icing under severe weather conditions.

Metabarcoding gillnets to assess unaccounted catch depredation or escape

AbstractGillnets are the world's most common net‐based fishing gear, comprising walls of light mesh designed to entangle fish. Gillnets are often retrieved with holes in the netting, which means some animals escape or are depredated unseen, but with some mortality. To effectively manage fisheries around the world, information is required on not only the harvested and discarded mortalities, but also problematic interactions and mortalities caused by the fishing gear and especially those involving protected species. This study sought to assess a novel method for determining such interactions by sampling five adjacent pieces of netting around each of ten holes in two bather‐protection polyethylene gillnets for environmental DNA or “eDNA”. Here we show that eDNA correctly identified all previously entangled‐and‐landed species. Also, eDNA from three uncaptured taxa were recorded: bull shark, Carcharhinus leucas, white shark, Carcharodon carcharias and dolphins (Delphindae), illustrating the potential to reveal previously cryptic gillnet interactions. We propose that as scientific methods evolve and autonomous real‐time DNA surveillance becomes routine, eDNA testing of fishing gears and vessels could provide a novel, complementary fishery‐monitoring tool.

Hybrid Metamaterials Perfect Absorber and Sensitive Sensor in Optical Communication Band

A subwavelength metamaterial perfect absorber (MPA) in optical communication band was proposed and tested using the finite-difference time-domain method. The absorber is periodic and comprises a top layer of diamond silicon surrounded by L-shaped silicon and a gold layer on the substrate. It can achieve dual-band perfect absorption, and one of the peaks is in the optical communication band. By changing the gap (g) between two adjacent pieces of L-shaped silicon, and the thickness (h) of the silicon layer, the resonance wavelength of absorption peak can be tuned. When the incident electromagnetic wave entered the absorber, the metamaterial absorber could almost completely consume the incident electromagnetic waves, thereby achieving more than 99% perfect absorption. The absorption peak reaches 99.986% at 1310 nm and 99.421% at 1550 nm. Moreover, the MPA exposed to different ambient refraction indexes can be applied as plasma sensors, and can achieve multi-channel absorption with high figure of merit (FOM*) value and refractive index (RI) sensitivity. The FOM* values at 1310 nm and 1550 nm are 6615 and 168, respectively, and both resonance peaks have highly RI sensitivity. The results confirm that the MPA is a dual-band, polarization-independent, wide-angle absorber and insensitive to incident angle. Thence it can be applied in the fields of optical communication, used as a light-wave filter and plasma sensor, and so on.

Splitting Necklaces, with Constraints

We prove several versions of Alon's necklace-splitting theorem, subject to additional constraints, as illustrated by the following results. (1) The “almost equicardinal necklace-splitting theorem” claims that, without increasing the number of cuts, one guarantees the existence of a fair splitting such that each thief is allocated almost the same number of pieces of the necklace (including “degenerate pieces” if they exist), provided the number of thieves $r=p^\nu$ is a prime power. By “almost the same” we mean that for each pair of thieves one of them can be given at most one piece more (one piece less) than the other. (2) The “binary splitting theorem” claims that if $r=2^d$ and the thieves are associated with the vertices of a $d$-cube, then, without increasing the number of cuts, one can guarantee the existence of a fair splitting such that adjacent pieces are allocated to thieves that share an edge of the cube. This result provides a positive answer to the “binary splitting necklace conjecture” in the case $r=2^d$ from Conjecture 2.11 in [M. Asada et al., SIAM J. Discrete Math., 32 (2018), pp. 591--610]. (3) An interesting variation arises when the thieves have their own individual preferences. We prove several envy-free, fair necklace-splitting theorems of various level of generality, as illustrated by the envy-free versions of (a) Alon's original necklace-splitting theorem, (b) the almost equicardinal splitting theorem, and (c) the binary splitting theorem, etc.

Winding up a finite size holographic superconducting ring beyond Kibble-Zurek mechanism

We studied the dynamics of the order parameter and the winding numbers $W$ formation of a quenched normal-to-superconductor state phase transition in a finite size holographic superconducting ring. There is a critical circumference $\tilde{C}$ below it no winding number will be formed, then $\tilde{C}$ can be treated as the Kibble-Zurek mechanism (KZM) correlation length $\xi$ which is proportional to the fourth root of its quench rate $\tau_Q$, which is also the average size of independent pieces formed after a quench. When the circumference $C \geq 10 \xi$, the key KZM scaling between the average value of absolute winding number and the quench rate $\langle|W|\rangle \propto \tau_Q^{-1/8}$ is observed. At smaller sizes, the universal scaling will be modified, there are two regions. The middle size $5\xi<C<10\xi$ result $\langle|W|\rangle \propto \tau_Q^{-1/5}$ agrees with a finite size experiment observation. While at $\xi<C\leq 5\xi$ the the average value of absolute winding number equals to the variance of winding number and there is no well exponential relationship between the quench rate and the average value of absolute winding number. The winding number statistics can be derived from a trinomial distribution with $\tilde{N}=C/ (f \xi)$ trials, $f\simeq 5$ is the average number of adjacent pieces that are effectively correlated.

Species diversity of soil mites (Acari: Mesostigmata) under different agricultural land use types

Mites are among the most important members of soil arthropod communities, because they are the most diverse in terms of ecological niche and behavior. Due to the sensitivity of soil mites to soil disturbance, their diversity and numbers can be used as ecological indices for assessing disturbances in ecosystems. To determine the effect of land use type on soil mite biodiversity, abundance and biodiversity indices of soil inhabiting mesostigmatic mites were evaluated at eight sites in Saman and Shahrekord, Iran, each site including two adjacent agricultural pieces of land: an orchard and a crop field. The biodiversity of mites was measured by several biodiversity indices and then compared by analysis of variance. The specimens collected belonged to 12 families, 17 genera and 24 species. The biodiversity index values calculated in different months showed that these indices were usually higher in warm months and in orchards than in cold months and crop fields. In the examined crop fields, the diversity index values were lower after harvesting, probably due to soil disturbance by agricultural machinery. There was a significant difference in the Shannon-Wiener's diversity index among different land uses. The maximum and minimum values of this index were recorded at the vine orchard (1.48) and wheat field (0.15) in an elm/wheat site at Shahrekord, respectively. The soil organic matter content was maximum in the vineyard (2.12%) and minimum in the wheat field (0.41%).

A User Cooperation Approach for Interference Cancellation in FDD Massive MIMO Systems

The performance of a massive multi-user Multi-Input Multi-Output (MIMO) system, operating in Frequency Division Duplex (FDD) mode, severely degrades under imperfect Channel State Information (CSI). Among the main challenges toward the acquisition of sufficiently accurate CSI at the transmitter is the issue of enormous CSI feedback overhead. In this paper, a novel interference cancellation strategy is proposed to alleviate the overhead. The concept of a device-to-device based interference cancellation strategy was hinted in some prior works but has not been fully exploited in the multi-user MIMO systems, especially when the number of antennas becomes large. Hence, this paper aims to exploit the potential of User Equipment (UE) cooperation to reduce the dependency of precoder at the transmitter to the accuracy of CSI. To do so, adjacent pieces of UE that experience correlated CSI are clustered in a similar group, jointly adjusting their receive antenna combining the weight vector to maximize the channel vector orthogonality. Simulation results show that the proposed strategy reduces the dependency of system performance on the accuracy of CSI feedback; moreover, compared to the conventional limited feedback strategy, a larger number of antennas can be deployed at the transmitter.

A characterization on separable subgroups of 3‐manifold groups

In this paper, we give a complete characterization on which finitely generated subgroups of finitely generated 3-manifold groups are separable. Our characterization generalizes Liu's spirality character on surface subgroups of closed 3-manifold groups. A consequence of our characterization is that, for any compact, orientable, irreducible and ∂-irreducible 3-manifold M with nontrivial torus decomposition, π 1 ( M ) is locally extended residually finite if and only if for any two adjacent pieces in the torus decomposition of M, at least one of them has a boundary component with genus at least 2.

Lateral Resistance of CLT Wall Panels Composed of Square Timber Larch Core and Plywood Cross Bands

Thinned, small larch logs have small diameters and no value-added final use, except as wood chips, pallets, or fuel wood, which are products with very low economic value; however, their mechanical strength is suitable for structural applications. In this study, small larch logs were sawed, dried, and cut into square timbers (with a 90 mm × 90 mm cross section) that were laterally glued to form core panels used to manufacture cross-laminated timber (CLT) wall panels. The surface and back of these core panels were covered with 12-mm-thick structural plywood panels, used as cross bands to obtain three-ply CLT wall panels. This attachment procedure was conducted in two different ways: gluing and pressing (CGCLT) or gluing and nailing (NGCLT). The size of the as-manufactured CLT panels was 1,220 mm × 2,440 mm, the same as that of the plywood panels. The final wall panels were tested under lateral shear force in accordance with KS F 2154. As the lateral load resistance test required 2,440 mm × 2,440 mm specimens, two CLT wall panels had to be attached in parallel. In addition, the final CLT panels had tongued and grooved edges to allow parallel joints between adjacent pieces. For comparison, conventional light-frame timber shear walls and midply wall systems were also tested under the same conditions. Shear walls with edge nail spacing of 150 mm and 100 mm, the midply wall system, and the fabricated CGCLT and NGCLT wall panels exhibited maximum lateral resistances of 6.1 kN/m (100%), 9.7 kN/m (158%), 16.9 kN/m (274%), 29.6 kN/m (482%), and 35.8 kN/m (582%), respectively.

Diversity of Planktonic Fauna of the Pechora River Delta

The structure and diversity of zooplankton of the mouth of the largest river in the European North, the Pechora, is studied. The river delta, an adjacent piece of the Pechora course, the estuary (Korovinskaya Bay), and lakes on its northern coast—in Timan Tundra—are in the studied region. In the delta of the Pechora River, the planktonic fauna is rich and unique in structure, in which rotifers (105 species) and cladocerans (55 species) prevailed; thermophilic invertebrates are present in zooplankton, but psychrophilic ones are revealed. Among copepods, brackish taxa and also a possible recent invader of the region (Eurytemora sp.) are found. The analysis of alpha and beta diversities of planktonic communities of the studied region shows the highest indexes for the delta water bodies relative to others and for the lake zooplankton in general. In our opinion, such diversity is due to a variety of environmental conditions and also features of gradients of the dominating environmental factors: temperature, anthropogenic, etc.

Incorporating Co‐Benefits and Environmental Data into Corporate Decision‐Making

AbstractThis case study deals with The Dow Chemical Company's (Dow) and the City of Midland, Michigan's decisions on how to restore two adjacent pieces of property. Dow aimed to restore a greenbelt area with historical issues, and the City of Midland aimed to restore a brownfield property that bordered Dow's greenbelt area. Dow has a stated goal to apply a “business‐decision process that values nature” and to deliver $1 billion in “value through projects that are good for business and good for ecosystems.” The City of Midland has developed a 20‐year Master Plan that includes goals for Midland to connect and maintain open spaces. In line with these goals, Dow and the City of Midland wanted to restore the greenbelt area and brownfield property by enhancing habitat and ecosystem services in a way that was also beneficial to Dow's bottom line and the residents of Midland, respectively. This case study presents three alternative restoration designs along with detailed financial cost and environmental data for each design. Students perform a cost‐benefit analysis, highlighting potential differences between how costs are calculated in a public setting relative to a private setting. In addition, students assess how the inclusion of important non‐financial environmental data may be used to inform decision making.

Solving Scramble Squares puzzles with repetitions

A Scramble Squares puzzle consists of nine square pieces with half of an image on each side. To solve the puzzle the pieces are arranged in a 3×3 grid so that sides of adjacent pieces form complete images. A repetition is a half-image that appears more than once on a piece. Previous research uses a graph-theoretical approach to establish necessary and sufficient conditions for solutions without repetitions to 2×2 Scramble Squares puzzles. We use a similar approach to establish necessary and sufficient conditions for solutions with repetitions to 2×2 Scramble Squares puzzles.

Influence of Leakage Flows on Hot Gas Ingress

One of the most important problems facing gas turbine designers today is the ingestion of hot mainstream gases into the wheel-space between the turbine disk (rotor) and its adjacent casing (stator). A rim seal is fitted at the periphery and a superposed sealant flow—typically fed through the bore of the stator—is used to prevent ingress. The majority of research studies investigating ingress do so in the absence of any leakage paths that exist throughout the engine's architecture. These inevitable pathways are found between the mating interfaces of adjacent pieces of hardware. In an environment where the turbine is subjected to aggressive thermal and centrifugal loading, these interface gaps can be difficult to predict and the resulting leakage flows which pass through them even harder to account for. This paper describes experimental results from a research facility which experimentally models hot gas ingestion into the wheel-space of an axial turbine stage. The facility was specifically designed to incorporate leakage flows through the stator disk; leakage flows were introduced axially through the stator shroud or directly underneath the vane carrier ring. Measurements of CO2 gas concentration, static pressure, and total pressure were used to examine the wheel-space flow structure with and without ingress from the mainstream gas-path. Data are presented for a simple axial-clearance rim-seal. The results support two distinct flow-structures, which are shown to be dependent on the mass-flow ratio of bore and leakage flows. Once the leakage flow was increased above a certain threshold, the flow structure is shown to transition from a classical Batchelor-type rotor-stator system to a vortex-dominated structure. The existence of a toroidal vortex immediately inboard of the outer rim-seal is shown to encourage ingestion.

TMOD-23. ESTABLISHMENT AND CHARACTERIZATION OF IDH1-MUTANT HYPERMUTATED GLIOMA CELL LINES

Low-grade gliomas can undergo hypermutation (HM) and malignant progression after treatment with temozolomide (TMZ). We and others have demonstrated that TMZ-associated HM is characterized by predominant C>T/G>A nucleotide transitions and mutation of mismatch repair (MMR) and PI3K pathway genes. Well-characterized models of HM glioma are needed to better understand the biology of HM tumors. We established three IDH1-mutant HM glioma cell lines from two post-treatment surgical specimens, two from an ATRX- and P53-mutant HM astrocytoma (P137), and one from a TERT promoter-mutant, 1p/19q co-deleted HM oligodendroglioma (P278). P137 cultures were established in serum-free conditions on laminin (GNS) and in 10% serum (ML). HM status was determined by exome sequencing. P137 GNS and P137 ML have 4,722 and 2,946 mutations, respectively, and represent independent HM events compared to each other and both spatially distinct and adjacent samples of uncultured tissue. MMR genes MSH4, MSH5, and MSH6 and PI3K pathway gene AKT3 have C>T/G>A mutations in P137 ML. In P137 GNS, MSH4, MSH5, MSH6, MLH1, MTOR, RPTOR, PIK3CA, and PTEN have C>T/G>A mutations. Both P137 ATRX-mutant lines are positive for alternative lengthening of telomeres, as measured by c-circle assay. Phylogenetic analysis shows that an independent tumor sample of P137 shares 524 mutations with the cell lines and has 1,872 private mutations. An immediately spatially adjacent piece of tumor shares 1,081 mutations with the GNS culture, only 47 mutations with the ML culture, and has 2,865 private mutations. P278 was established in serum-free conditions, has 5,752 mutations, and shares 4,875 of these mutations with two independent pieces of the tumor, including C>T/G>A mutations in MLH1, MSH2, MTOR, RPTOR, and PIK3R1. All cell lines maintain an IDH1 R132H heterozygous mutation through months of in vitro growth, produce 2-hydroxyglutarate, and demonstrate anchorage-independent growth. These cell lines provide novel tools for modeling hypermutated oligodendroglioma and astrocytoma.