The role of Glucose‐6‐phosphate dehydrogenase(G6PD), NADPH producing enzyme, has been received a great attention in metabolic disorder such as obesity, diabetes and atherosclerosis. Previously, we have demonstrated that increased expression of G6PD in adipose tissue of obese animals provokes insulin resistance and stimulates inflammatory response and ROS production in adipocytes. To further understand the role of G6PD in metabolic disorders, we investigated G6PD in macrophages. Interestingly, the level of G6PD expression in stromal vascular fraction of adipose tissue was increased in obese animals, which exhibit enhanced infiltrating monocyte and macrophages. Additionally, the expression of G6PD in macrophage was stimulated by inflammatory signals such as LPS. Moreover, overexpression of G6PD in macrophage stimulated the expression of inflammatory genes including MCP‐1, IL‐6 and iNOS, implying that enhanced G6PD activity is associated with augmentation of inflammatory response in adipose tissue of obese subjects. Together, it is plausible to speculate that abnormal increase of G6PD in macrophage would modulate expression profile of pro‐inflammatory cytokines, which could deteriorate whole body inflammatory signaling in obese subjects.This work was supported in part by grants from the Molecular and Cellular BioDiscovery Research Program (M10748000258‐07N4800‐25810) and the National Research Laboratory Program (R0A‐2004‐000‐10359‐0) of Korea Science and Engineering Foundation (KOSEF). J.B.K. and M.H. was supported by a BK21 Research Fellowship from the Ministry of Education and Human Resources Development.