Helical microtubes are commonly used in micro-electronic cooling techniques and micro-heat exchangers because of the creation of secondary flows, which leads to greater temperature and velocity gradients. It is of great significance to further improve the overall efficiency of the helical microtubes so as to diminish energy consumption. This experimental work mainly focuses on exergy analysis of air flow through adiabatic helical microtubes with circle, triangle, square, and pentagon geometries with circular cross section. The temperature rises due to viscose dispassion and pressure drops have been measured for all adiabatic helical coils in the laminar flow range. To identify irreversibility of flow, rate of air flow, coil diameter and Dean number are varied to investigate their influences on the entropy generation. Also, the second law of thermodynamics was applied to recognize exergy efficiencies and to determine true magnitudes of exergy losses. Results indicate that entropy generation increases by increasing the flow rate and the coil diameters in all geometries, however, the exergy efficiency decreases. By considering geometry, in constant flow rate and identical coil diameter, the highest entropy generation can be approximately observed in the triangles, squares, pentagons, and circles, respectively. Moreover, the proportions of theoretical exergy losses to actual exergy losses have been computed for all geometries in order to probe the viscous heating effects and it is found that the predicted exergy losses distinguish substantially from measured amounts due to viscose dispassion.