Adhesion Rate Research Articles

Aurelia aurita jellyfish collagen: Recovery properties

Wound and burn healing is a complex physiological process that can be facilitated by medications based on marine collagen. In this regard, biomass of the Aurelia aurita jellyfish is a promising alternative source of medical collagen. As the global incidence of burns and wounds continues to grow, new healing methods have become a relevant area of medical science. This study featured acetic acid as a means of marine collagen extraction from A. aurita biomass. The physical and chemical properties of jellyfish collagen were determined gravimetrically and included such indicators as water solubility and water holding capacity. The molecular weight was defined by gel electrophoresis. The spectral studies relied on the method of UV spectroscopy. The regenerative experiments included such parameters as cytotoxicity, antioxidant properties, adhesion, and wound healing rate, as well as a quantitative PCR analysis. The optimal conditions for maximal collagen yield were as follows: 0.5 M acetic acid and 48 h extraction time. However, the collagen yield was very low (≤ 0.0185%). The high water holding capacity showed good prospects for A. aurita collagen to be used as hemostatic sponge. The acid-soluble collagen sample had a molecular weight of 100–115 kDa, which made it possible to classify it as type I. A. aurita jellyfish collagen revealed no cytotoxic properties; it had no effect on adhesion, migration, and proliferation of keratinocytes, neither did it affect the expression of cell differentiation markers. The wound healing model proved that the marine collagen had regenerative properties as it was able to increase the wound healing rate by 24.5%. Therefore, collagen extracted from the biomass of A. aurita jellyfish d emonstrated good p rospects for cosmetology and regenerative medicine.

Growth and shrinkage of tissue sheets on substrates: buds, buckles, and pores

Abstract Many tissues take the form of thin sheets, being only a single cell thick, but millions of cells wide. 
These tissue sheets can bend and buckle in the third dimension. In this work, we investigated the growth and shrinkage of suspended and supported tissue sheets using particle-based simulations. We construct a minimum model, combining particle-based tissue growth and meshless membrane models, to simulate the growth of tissue sheets with mechanical feedback. Free suspended growing tissues exhibit wrinkling when growth is sufficiently fast. Conversely, tissues on a substrate form buds when the adhesion to the substrate is weak and/or when the friction with the substrate is strong. These buds undergo a membrane-mediated attraction and subsequently fuse. The complete detachment of tissues from the substrate and straight buckled bump formation are also obtained at very weak adhesion and/or fast growth rates. In the tissue shrinkage, tissue pores grow via Ostwald ripening and coalescence. The reported dynamics can also be applied in research on the detachment dynamics of different tissues with weakened adhesion.

Design and synthesis of quorum-sensing agonist for improving biofilm formation and the application of Acidithiobacillus thiooxidans in bioleaching

IntroductionCurrently, there are few investigations on the effect of a synthetic exogenous quorum sensing (QS) agonist on the bioleaching rate of Acidithiobacillus thiooxidans (A. thiooxidans).MethodsWe created AHL (N-acyl-homoserine lactone) analogues and investigated their effects on A. thiooxidans biofilm formation, adsorption kinetics, bioleaching, and mechanism.ResultsThe findings revealed that N-(3-thiolactone)- dodecylamine (Y3) significantly increased the biofilm formation of A. thiooxidans in 96-well plates and sulfur sheets. Adsorption tests revealed that Y3 increased the adhesion rate, adsorption constant, and adsorption efficiency. Bioleaching tests indicated that Y3 boosted bioleaching efficiency, with Ni2+ and Cu2+ bioleaching rates increasing by 49.13% and 33.03%, respectively. Transcriptomic analysis revealed that Y3 increased genes associated with QS pathways and biofilm formation, particularly afeI, which was dramatically elevated 42 times.DiscussionThe study laid the groundwork for a better understanding of the mechanics of A. thiooxidans biofilm formation, which could help improve the potential application of A. thiooxidans in bioleaching.

Zedoarondiol inhibits monocyte adhesion and expression of VCAM and ICAM in endothelial cells induced by oxidative stress

Zedoarondiol, a newly discovered compound derived from the roots of zedoary turmeric, a traditional Chinese herb, has demonstrated potential in reducing inflammation of the vascular endothelium and safeguarding it from harm. Nonetheless, the precise mechanism underlying these effects remains to be elucidated. In this study, we established a model of HUVEC injury induced by hydrogen peroxide. We observed whether Zedoarondiol could reduce the adhesion and transendothelial migration (TEM) of inflammatory cells by inhibiting the expression of VCAM-1 and ICAM-1 in HUVECs. The research findings indicate that utilising Zedoarondiol resulted in a significant reduction in the adhesion rate of THP1 cells to HUVECs, leading to a more condensed cytoskeletal structure. Moreover, Zedoarondiol demonstrated a decrease in NF-κBβ-Ser536 phosphorylation levels in H2O2-stimulated human umbilical vein endothelial cells, resulting in a hindered capacity to bind to target genes like ICAM-1 and VCAM-1, This findings may provide a new pharmacological basis and scientific evidence for Zedoarondiol to slow the atherosclerosis progression by maintaining endothelial function.

B-037 Zeon original coating technology COP microplates for cell imaging

Abstract Background Zeon has developed high quality microplate made of Cyclo Olefin Polymer (COP) and special coat reagent for cell culture for Aurora microplates. The new 96 well-microplates have best-in-class properties for analysis of cultured cells can reduce the background of fluorescence images absolutely and excellent bottom flatness for observation of target. This enables to obtain high-precision data for cell analysis. Also, we developed Zeon original coating (ZOC) reagent specialized for COP surface. It is suitable for various cells including neurons derived from human induced pluripotent stem cells (iPSC), primary cells, general cell lines, and so on. ZOC coated COP microplates (ZOC plates) are fully-precoated and ready-to-use. Methods We evaluated the following points regarding ZOC plates. The cells used were derived from human iPSC. [1. Storage stability] ZOC plates were stored at room temperature for a long time. After that, cardiomyocyte cells were cultured for 3 days on the plates. The cell adhesion and beat rate were evaluated. [2. Neurite extension] Dopaminergic neurons were cultured for 14 days, then the neurite extension was evaluated. For comparison, a conventional poly-D-lysine* (PDL) coating was used. [3. Lot-to-lot variation] COP microplates coated with different lots of ZOC reagents were prepared. Glutamatergic neurons were cultured for 14 days on those plates, then the neurite extension and cell morphology were evaluated. *The typical coating reagent applied for neuronal culture. Results [1.] ZOC plates stored over 12 months at room temperature showed excellent cell adhesion, and the beat rate was equivalent to prepared just before use. [2.] ZOC plates tended to extend neurites more than PDL coated plates. [3.] No variation between lots was observed on ZOC plates regarding the neurite extension and cell morphology. Conclusions We suggest applying the combination of high accuracy for cell imaging using COP microplates and high performance for cell culture using ZOC can provide high precision data at bioassay research. This would be a significant value for development of drug screening processes at pharmaceutical company.

A comparative study on the efficacy of different treatment methods for preventing vocal cord adhesion after laser treatment for glottic cancer

Objective:Comparing the primary tumor control, vocal function recovery, postoperative adhesion rate and degree of adhesion in early glottic cancer involving the anterior commissure treated with CO₂ laser staged and lateral surgery, one-stage surgery combined with laryngeal stent placement, and simple CO₂ laser excision. Methods:This study focuses on 83 patients with T1-2N0M0 stage glottic squamous cell carcinoma involving the anterior commissure who underwent CO₂ laser treatment. The study was divided into three groups: Group A with 15 cases, treated with staged resection surgery; Group B with 18 cases, treated with one-stage surgery combined with the placement of a silicone laryngeal stent; and Group C with 50 cases, treated with simple CO₂ laser excision. The Voice Handicap Index-10（VHI-10）, the GRBAS auditory-perceptual assessment, and the maximum phonation time（MPT） were used to evaluate the vocal function of the patients before and six months after surgery. The degree of vocal cord adhesion was assessed using the Cohen classification of vocal cord adhesion. Statistical analysis was performed to determine the differences in each indicator before and after surgery, and the primary tumor control rates among the three groups. Results:Local recurrence occurred in 1 case each in Groups A and B, and in 4 cases in Group C, with no distant metastasis observed. Postoperative vocal cord adhesion of varying degrees occurred in a total of 77 cases, with an adhesion rate of 73.3%（11/15） in Group A, 88.9%（16/18） in Group B, and 100%（50/50） in Group C. The postoperative vocal cord adhesion rate and degree in Group C were significantly higher than in Groups A and B. The postoperative VHI-10 scores in all three groups were significantly increased compared to preoperative scores（P<0.05）, and when compared between groups postoperatively, Group C was significantly worse than Groups A and B （P<0.05）. The postoperative maximum phonation time（MPT） in Group C was significantly reduced compared to preoperative and was markedly shorter than that of Groups A and B postoperatively（P<0.05）. The postoperative grades of G（Grade） and R（roughness） in Group C were significantly higher than preoperatively, indicating a noticeable deterioration in voice quality, and were also significantly worse than those postoperatively in Groups A and B, with all differences（P<0.05）. Conclusion:For early glottic cancer involving the anterior commissure, choosing staged surgery or one-stage surgery combined with the placement of a silicone anterior commissure laryngeal stent were better than simple laser tumor excision in terms of secondary vocal cord adhesion and voice function preservation.

Evaluation of Postoperative Adhesions in a Subsequent Cesarean Section Following the Use of Dextrin Hydrogel Spray as Adhesion Barrier for Laparoscopic Myomectomy

Objectives: Laparoscopic myomectomy (LM) is a procedure with a high rate of postoperative adhesions, which can lead to complications such as bowel obstruction and subsequent surgical problems, making anti-adhesion measures important. Various anti-adhesion materials are already on the market and have shown efficacy, but dextrin hydrogel spray (AdSpray™), an anti-adhesion material launched in 2017, has not yet been reported in the field of gynecology, although there are papers showing its usefulness in the surgical field such as repeat hepatectomy and ileostomy closure. Thereby, we investigated the postoperative status of AdSpray™ in LM. Materials and Methods: We report 24 cases in which AdSpray™ was used at Teine Keijinkai Hospital from 2018 to 2021 for LM, followed by cesarean section (CS) from 2019 to 2022. Adverse effects related to AdSpray™ and the presence of uterine adhesions in CS were examined. Results: Adhesions were observed in 4 (16.7%) cases, none of which resulted in significant adverse effects of AdSpray™. Conclusion: AdSpray™ was effective in preventing adhesion and may be an option as an anti-adhesion material in LM.

The synergistic potential of polyethylene glycol 400 for the control of Hyphantria cunea larvae by Beauveria bassiana

The efficacy of entomopathogenic fungi as pest control agents is constrained by both their physiological state and external environmental factors. This study identified synergists capable of enhancing the insecticidal activity of Beauveria bassiana (Bb) and investigated the underlying synergistic mechanisms. Our results found that among 6 potential synergists, polyethylene glycol 400 (PEG) and trehalose significantly improved Bb's lethality against Hyphantria cunea larvae, with PEG demonstrating the most pronounced effect. PEG treatment markedly increased Bb spore adhesion and germination rates, while spore hydrophobicity and growth rates remained unaffected. Moreover, PEG-treated spores exhibited higher thermal tolerance compared to untreated ones. In the Bb + PEG treatment group, the hemocyte count, encapsulation and melanization activities, and the expression of related regulatory genes were significantly lower than those in the Bb treatment group. Additionally, pathogen recognition, signal transduction, and humoral immunity effector genes expression were markedly suppressed in the Bb + PEG group. A significant reduction in the content of total amino acids, free fatty acids, glucose, and trehalose, alongside decreased expression of key regulatory genes in the tricarboxylic acid cycle and glycolysis pathways, was observed in the Bb + PEG treatment group. Furthermore, PEG enhanced Bb-induced apoptosis in H. cunea larvae, as evidenced by the upregulation of apoptosis-related genes. Notably, PEG alone did not significantly impact the innate immunity, energy metabolism, or apoptosis in H. cunea larvae. Overall, PEG exhibits considerable potential in amplifying Bb's insecticidal activity by directly optimizing spore performance and indirectly modulating the larvae's innate immunity, energy metabolism, and apoptosis.

Ultralight 3D silk nanofiber scaffolds with enhanced hemostatic properties and biocompatibility for rapid hemostasis

Developing an effective hemostatic material that combines excellent biocompatibility with high blood absorption capacity remains a critical clinical challenge. In this study, ultralight three-dimensional (3D) silk fibroin (SF) scaffolds, composed of continuously interconnected nanofibers, are introduced as a novel hemostatic material. The distinctive fluffy nanofiber architecture endows the scaffolds with a remarkable porosity of 78.9 % and exceptional liquid absorption capacity. The integration of montmorillonite (MMT) further enhances the hemostatic properties of the scaffolds, while SF naturally promotes the intrinsic coagulation pathway. The scaffolds exhibit an impressive blood absorption capacity of 1687.7 %. In vitro evaluations indicate that the SF nanofiber scaffolds exhibit a blood clotting index rate of 24.6 %, a hemolysis ratio of less than 4 %, and outstanding biocompatibility. The blood coagulation time is significantly reduced from 449 s to 230 s. Additionally, the scaffolds show adhesion rates to erythrocytes and platelets of 52 % and 45.9 %, respectively. The nanofiber scaffolds aggregate and activate platelets extensively, accelerating the formation of platelet emboli, which collectively contribute to their superior hemostatic performance. Consequently, these nanofiber scaffolds hold substantial promise as absorbable hemostatic agents for achieving rapid hemostasis in clinical applications.

Factors Affecting the Adhesion of Flour Particles to Stainless-steel Surfaces and Vacuum Dry-cleaning

Recent outbreaks and recalls linked to flour-based products have highlighted the need for improved cleaning methods in low-moisture environments. The factors affecting adhesion forces of flour particles, and the vacuum cleaning methodologies to overcome these forces, need to be better understood. The objectives of this study were to: (1) Measure electrostatic charge build-up in flour under different environmental conditions (20, 40, 60% relative humidity at room temperature), (2) quantify how powder size (US standard No. 60–80 or 80–100 mesh), electrostatic charge (charged and uncharged), and relative humidity impact the force required to remove the powder from an electropolished 304 stainless steel coupon (8 × 8 × 0.2 cm), and (3) determine the most effective vacuum nozzle angle (0, 45, 90° relative to the surface) for cleaning. Chargeability (nC) of flour samples was assessed using Faraday cup electrometry, while the surface adhesion force of the flour particles was measured using a custom-built impact tester. The surface cleanliness after vacuum treatments was assessed using ATP (adenosine triphosphate) swabs and a luminometer. Charged flour samples at 20% relative humidity (RH) exhibited a significantly higher charge compared to those at 40 and 60% RH. Within the 60–80 mesh range, charged flour showed higher adhesion rates than uncharged samples at both 20 and 40% RH. However, in the 80–100 mesh range, charged flour did not show a significant difference in adhesion when compared to uncharged samples at any RH level. Additionally, at 60% RH, surface residues measured by ATP were significantly lower for a vacuum angle of 90° than for 0° across both 60–80 mesh and 80–100 mesh size ranges of wheat flour. The vacuum cleaning treatment proved capable of overcoming the increase in adhesion from triboelectric forces; however, trace flour residues were still detected on stainless steel surfaces postvacuuming, indicating that vacuuming alone may be insufficient.

Evaluation of in vitro probiotic properties and colonization of Brevibacillus Laterosporus S62-9 in the intestine of broiler chickens

Brevibacillus laterosporus has been used as a direct-fed microbial additive in chicken feed owing to its strain safety and the ability to produce broad-spectrum antimicrobial peptides. To assess the efficacy and persistence of Brevibacillus laterosporus, it is necessary to track the supplied strains throughout the gastrointestinal tract. This study aims to evaluate the in vitro probiotic properties of Brevibacillus laterosporus S62-9 and its capacity to colonize and persist in the intestines of broiler chickens. Results indicated that the spores of Brevibacillus laterosporus S62-9 exhibited higher tolerance to the digestive condition, and higher adhesion rate to mucus and intestinal cells compared to vegetative cells. The spores remained dormant during digestion in the crop and stomach but germinate in the small intestine with a germination rate exceeding 70%. Subsequently, the whole genome of S62-9 was sequenced, and specific primers were designed and validated based on comparative genome analysis. The quantitative PCR-based method had a detection limit ranging from 2.71 to 7.71 log10 CFU/mL in pure culture and from 3.25 to 9.25 log10 CFU/g in broiler cecal content. In vivo colonization assay indicated that the Brevibacillus laterosporus S62-9 can transiently colonize the broiler cecum. In summary, our results indicated that the Brevibacillus laterosporus S62-9 spores exhibited high tolerance to the digestive tract environment, germinated in the intestine, and establish colonization in the cecum of broilers. The findings provide a theoretical basis for the future application of Brevibacillus laterosporus S62-9 in broiler farming.

Synthesis of N, N,O and O-carboxymethyl chitosan derivatives of controllable substitution degrees and their utilization as electrospun scaffolds for bone tissue engineering

Most chitosan (CS) carboxymethylation approaches are weighed down by insufficient description protocols regarding the reaction specificity and the degree of substitution (DS). Here, we provide three carboxymethylation protocols of enhanced specificity towards the amine (N-), amine/hydroxy (N,O-) and hydroxy (O-) groups of CS. The DS for all samples was found to be ∼70 %, as confirmed by NMR analysis. To illustrate the modified materials' potential in bone tissue regeneration, each derivative was blended with poly(vinyl alcohol) to prepare scaffolds via electrospinning. Both materials and electrospun membranes were characterized in terms of their physicochemical properties by Fourier transform infrared spectroscopy, thermogravimetric analysis, differential scanning calorimetry and water contact angle measurements. The electrospun membranes' swelling ratio, degradation, tensile strength, as well as morphology were also examined through scanning electron microscopy before and after heat treatment at 120 °C, which was used as a method of physical stabilization, leading to significantly enhanced degradation rate and mechanical strength. The three electrospun scaffold types were loaded with MC3T3-E1 pre-osteoblastic cells and their cell adhesion, viability and growth rate were assessed. Finally, osteogenic differentiation was examined by means of alkaline phosphatase activity measurement, calcium mineralization and formation of extracellular matrix markers, with all materials showing promising prospects.

A Study on the Application of Lining Adhesives to Canvas Reinforcement Treatment(Patching)

Canvas patching utilizes various adhesives for localized reinforcement treatment, yet evaluations of their properties remain insufficient. This study presents surface changes and physical properties observed when seven types of lining adhesives, selected from prior research, were applied. Evaluations included contact angles, color changes, surface alterations, and tensile strength. Results indicated that glue paste was highly susceptible to moisture and exhibited decreased flexibility with environmental changes, while Beva film showed the most significant reduction in adhesion. Both glue paste and Beva film were sensitive to color changes, primarily displaying yellowing and browning. Plextol B500 demonstrated the greatest increase in adhesion and flexibility under environmental stress. Welding powder exhibited high adhesion but lower strain rates, causing notable deformations across the canvas. Within the same adhesive composition, higher hydrophilicity correlated with reduced yield stress and maximum strain, suggesting that hydrophilicity affects adhesion and flexibility. Adhesives with lower thermal decomposition and glass transition temperatures experienced greater changes in adhesion and flexibility. Rapid changes in adhesion following localized reinforcement treatment can lead to issues like peeling of reinforcing fabric or deformation of the canvas.

Clean intermittent catheterization in multiple sclerosis patients: An adherence and long-term follow-up study

IntroductionClean Intermittent Catheterization (CIC) is considered as a gold standard of treatment for bladder emptying disorders. A large amount of literature on CIC for patients suffering from neurological disorders is available, but there is a lack of research specifically concerning multiple sclerosis (MS) patients. Our primary outcome was to determine the characteristics of our population (sex, EDSS and age when CIC was introduced). Our secondary outcomes were to determine adherence of CIC. MethodAs part of a multicenter, observational, retro-prospective study, data was collected from neuro-urologist consultation reports, and extracted from bladder diaries between 01/01/2000 and 31/03/24. MS patients, over 18 years, with the indication of CIC were included. Results195 patients (72.3% women) were included, with a mean age of 49 years old. The median of follow-up was 9 years. Median EDSS at the start of the study was 5.5. There was an adherence rate of 65.1%. Urinary leakage was present in 74.2% of patients prior to CIC and 31.6% following CIC. ConclusionCatheterization is mainly offered to patients with an EDSS between 0 and 7. Rate of adhesion is encouraging, with most patients still continuing to use CIC by the end of follow-up. During the follow-up, we observed a reduced leakage rate but CIC alone can not explain this improvement. Following studies should include a list of constraints and reasons of halted CIC.

A marker-free genetic manipulation method for Glaesserella parasuis strains developed by alternately culturing transformants at 37°C and 30°C

BackgroundGlaesserella parasuis (G. parasuis) is the causative agent of Glässer’s disease, which causes significant economic losses in the swine industry. However, research on the pathogenesis of G. parasuis has been hampered by the lack of a simple and efficient marker-free knockout system.ResultsIn this study, a marker-free knockout system was developed for G. parasuis using a temperature-sensitive vector. By alternating the incubation of transformants at 30°C and 37°C, we optimized the screening process for this system. The system was successfully applied to knockout the KanR cassette from JS0135ΔnanH::KanR, achieving a knockout efficiency of 90% in the final round of screening. To confirm that temperature variation was a key factor, we proceeded with knocking out the nanH and apd genes in the CF7066 strain. The knockout efficiency reached up to 100%, with the shortest screening time being only four days. The knockout of the nanH gene resulted in a significant reduction in the growth vitality of the strains, while the knockout of the apd gene led to an approximate 56% improvement in the adhesion rate. Additionally, we observed that the expression of recombinant genes in transformants was higher at 30℃ than at 37℃, with the recC gene being upregulated approximately 7-fold. In contrast, there was almost no difference in the expression of recombinant genes between 30℃ and 37℃ in the wild-type strains. This discrepancy was likely due to an elevated copy number of target plasmids at 30℃, which may have resulted in the enhanced expression of recombinant genes.ConclusionsIn conclusion, this newly developed gene knockout system for G. parasuis presents a valuable tool for advancing research on this organism.

Influence of the shortened warming protocol on human blastocyst viability: an in vitro experimental study

Research QuestionDoes the shortened warming protocol impact the cell viability and outgrowth competence of human vitrified blastocysts warmed with or without fatty acids (FAs)? DesignIn this study, 326 discarded vitrified human blastocysts donated for research by consenting couples were used. The blastocysts were randomly allocated to five groups depending on the warming solutions, protocols, and recovery culture media: the control-conventional, control-shortened, FA-conventional, FA-shortened, and FA-shortened/recovery culture with FA (RF) groups. The blastocysts were warmed with or without FAs following the manufacturer's instruction (conventional method) or using the shortened method, in which blastocysts were immersed in a thawing solution for 1 min and then cultured in the recovery medium for 2 h. The embryo volume recovery, cell viability, intracytoplasmic lipid droplets, and outgrowth competence were evaluated. ResultsThe degree of blastocyst volume recovery was significantly higher after shortened warming than after conventional warming. Cell membrane collapse was observed during the shortened warming. The blastocyst survival rate and expansion status after the recovery culture were comparable among the five groups. However, the proportion of necrotic cells was increased in the control-shortened, FA-shortened, and FA-shortened/RF groups. The adhesion rates were comparable among all the groups; however, the outgrowth area was significantly higher in the FA-conventional group than in the other groups. ConclusionsThe efficacy of the shortened warming protocol depended on the warming solutions used. The shortened protocol effectively improves the daily workflow; however, each laboratory should examine its impact thoroughly before introducing it to prioritise clinical outcomes.

Early diagnosis to avoid invasive treatment in cardiac migration of a ventriculoperitoneal catheter: a qualitative systematic review and Weibull analysis of case reports

OBJECTIVE The migration of distal catheter after ventriculoperitoneal shunt placement is a rare but significant complication. Especially in a case of cardiac migration, open-heart surgery or catheter intervention may be required. The authors encountered a case of cardiac migration that fortunately could be treated by withdrawal. A systematic review of cardiac migration was performed to clarify when and how migration was diagnosed and why invasive treatments were required. Based on the collected cases, a Weibull analysis of the latency until diagnosis was performed to examine whether cardiac migration is caused by an initial factor and to compare the result with the other migration sites such as gastrointestinal tract or urinary tract. METHODS A qualitative systematic review was performed according to the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) guidelines. A database of case reports was created by searching PubMed and Scopus with the keywords "ventriculoperitoneal AND shunt AND migration" (last search date: April 2022). Whether the occurrence rate of migration is constant over time was examined by fitting a Weibull distribution. RESULTS A total of 339 articles of all migration sites were identified. Among them, 36 articles reporting 38 cases of cardiac migration were considered eligible. A total of 39 cases including the authors’ case were reviewed qualitatively. When classifying the cases by their latency to diagnosis, the rates of pulmonary thrombosis and of cardiac adhesion were higher in the delayed group (≥ 1 year) than in the early group (≤ 1 month). The rate of open chest surgery was higher in the delayed, intermediate, and early groups, in that order. In the Weibull analysis, the shape parameter (β) was less than 1, indicating that the occurrence rate of cardiac migration was initially high, followed by a decline. The finding supports the hypothesis that migration results from an intraoperative vascular injury. Note that these findings are subject to bias given that they are derived from case reports. CONCLUSIONS In light of the previous reports, the latency until diagnosis of cardiac migration was associated with the rate of thrombosis and adhesion, which resulted in escalation to invasive treatment. Early diagnosis will prevent invasive treatment because most cases are caused by initial factors, as the Weibull analysis showed.

Enhanced hemocompatibility and antioxidant efficacy of chitosan-PCL nanofibrous mats with olive oil and honey nika cream for wound healing applications

This study introduces an innovative approach utilizing electrospun scaffold matrices comprising polycaprolactone (PCL) and chitosan (CS), enriched with active ingredients of olive and Honey Nika cream (NC). These matrices were assessed for their potential as advanced wound dressings. The microstructures shown uniformly smooth nanofibers with diameters ranging from 200 to 250 nm across various formulations. XRD results demonstrated that incorporating NC and CS simultaneously, increased the crystallinity of PCL. The thermal analysis illustrated that although the addition of NC and CS declined the thermal stability of PCL fibers, but had no significant influence on the microstructure of the PCL fibers, and scaffolds have suitable chemical stability. FTIR analysis revealed the presence of olive oil, honey, and propolis components in the scaffolds. The developed nanofibers demonstrated advantageous properties such as prolonged NC release due to the incorporation of chitosan, enhanced cell adhesion, optimal swelling and degradation rates, minimal hemolytic activity (less than 0.44 %), and suitable antioxidant capabilities (approximately 50.97 for PNC mat). Further corroborated by in vitro cytocompatibility assessments via MTT assays were shown biocompatibility of the prepared nanofibrous mats. This research highlights the promise of PCL/CS electrospun nanofbrous scaffold for wound healing applications.

Application of buried auricular point combined with Wenjing Sanhan prescription in arteriosclerosis obliterans patients with resting pain.

Research on the combined use of ear acupoint embedding beans and warming meridians with cold-dispersing formulas for alleviating resting pain in patients with arteriosclerosis obliterans (ASO) remains limited. To explore the therapeutic efficacy of auricular point embedding beans combined with Wenjing Sanhan prescription in alleviating resting pain in patients with lower-limb ASO. A total of 100 patients with ASO experiencing resting pain who were treated at our hospital from January 2022 to January 2023 were enrolled. They were randomly allocated into two groups using a double-blind approach. The control group was treated using a warming meridian with a cold-dispersing formula, while the study group received additional treatment with ear acupoint embedding beans. The clinical efficacy, ankle-brachial artery pressure ratio, hemorheological indicators, and traditional Chinese medicine symptom scores were compared between the two groups. The clinical efficacy rate in the study group was significantly higher (94.00%) than that in the control group (72.00%, P < 0.05). Moreover, the ankle-brachial artery pressure ratio was significantly higher in the study group after treatment (P < 0.05). Hemorheological parameters, including whole blood viscosity, plasma viscosity (1.83 ± 0.11) mPa/s, fibrinogen levels (3.30 ± 0.21) g/L, platelet adhesion rate (49.87% ± 10.51%), and erythrocyte aggregation index (1.79 ± 0) were improved in the study group compared to the control group. In addition, the scores for decreased skin temperature (1.41 ± 0.26), intermittent claudication (1.30 ± 0.20), and resting pain (1.23 ± 0.31) were significantly lower in the study group than those in the control group (all P < 0.05). The level of oxidative stress in the study group also exhibited significant improvement (P < 0.05), and the levels of inflammatory factors were considerably lower than those in the control group. The combination of ear point embedding beans and Wenjing Sanhan prescription demonstrates promising clinical efficacy in alleviating resting pain associated with ASO.

In vitro characterization of probiotic potential of Lactobacillus plantarum CM49 against selected cattle mastitogens

BackgroundBovine mastitis results in significant economic losses for the dairy industry globally due to milk production losses and decreased herd efficiency. This research aimed to isolate, select, and characterize indigenous lactobacilli with probiotic properties. A total of 40 lactobacilli were isolated from healthy milk samples of cattle and identified at the species level through 16S rDNA sequencing. All isolates were initially screened for antimicrobial activity, and selected isolates underwent in vitro assessment of probiotic properties.ResultsAmong the lactobacilli isolates, varying levels of activity (9 to 19 mm) against cattle mastitogens; Stapylococcus aureus (Staph. aureus), Escherichia coli (E. coli) and Streptococcus dysgalactiae (Strep. dysgalactiae) were observed in the well diffusion assay. These isolates demonstrated auto-aggregation (ranging from 14.29 ± 0.96% to 62.11 ± 1.09%) and co-aggregate (ranging from 9.21 ± 0.14% to 55.74 ± 0.74%) with mastitogens after 2 h. Lactobacillus (Lb.) plantarum CM49 showed sensitivity to most antibiotics tested and exhibited strong inhibitory effects, with mean log10 reductions of 3.46 for Staph. aureus, 2.82 for E. coli, and 1.45 for Strep. dysgalactiae in co-culture experiments. Furthermore, Lb. plantarum CM49 significantly decreased the adhesion rate of mastitogens on the bovine mammary cell line and mouse model, demonstrating its potential effectiveness in preventing mastitis.ConclusionIt is concluded that Lb. plantarum CM49 has remarkable probiotic potential with activity against cattle mastitogens in the laboratory and cell culture and competitively excludes mastitogens from bovine mammary cells and ameliorates Staph. aureus-induced mastitis in mice.