PURPOSE: We compared the efficacy of different contact lens disinfection systems to eliminate adenovirus. METHODS: Laboratory study evaluating the elimination of adenoviral ocular isolates by contact lens disinfection systems. Hard (gas permeable) and soft contact lenses were contaminated with adenovirus serotypes 8 and 19, and then they were disinfected with chemical, hydrogen peroxide, and heat sterilization systems. The survival of the adenovirus was determined by the shell vial technique. RESULTS: Adenovirus survived chemical and hydrogen peroxide disinfection but not heat sterilization. CONCLUSION: Because heat sterilization is not readily available to sterilize adenovirus contaminated contact lenses, it may be prudent for patients with adenoviral keratoconjunctivitis to dispose of unclean contact lenses.