Adenosine In Inhibition Research Articles

THE STUDY OF THE STABILITY OF THE GEL FORMULA AND THE ABSORPTION IN THE HAIR OF BLUE PEA EXTRACT GEL AND CRC EXTRACT GEL

s. PHYTOPHARM 2012 M 90 Obzory po kliniceskoj farmacologii i lekarstvennoj terapii [Reviews of clinical pharmacology and drug therapy] ТОМ 10/2012/2 of 250mg/ml showed a potent inhibition effect toward platelet aggregation. Compared with other extracts, water extract exhibited the strongest inhibition as well as adenosine (50uM/ml) as a positive control. in Addition to this, methanol extracts showed relatively similar inhibition pattern with adenosine. finally, this initial finding has provided the evidence of Phyllantus niruri l. action in platelet functions. Table. Platelet Aggregation inhibition Adenosine Water Extract hexane Extract Chloroform Extract Ethanol Extract Methanol Extract inhibition ( %) 68.6 ± 4.3 72.7 ± 3.3 40.1 ± 5.6 44.7 ± 2.7 43.5 ± 6.6 68.8 ± 6.7 Values are mean ± SE (n = 8). Platelet counts was 1.5x108 cells/ml PRODUCTION OF ANTI–MADECASSOSIDE POLYCLONAL ANTIBODY AND ITS APPLICATION FOR QUALITY CONTROL OF CENTELLA ASIATICA (L.) URBAN © PutalunWaraporn , Tassanawat Patcharin , Yusakul Gorawit , Sritularak Boonchoo , Juengwatanatrakul Thaweesak , Tanaka Hiroyuki 5 Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, 40002, Thailand. Research Group for Pharmaceutical Activities of Natural Products using Pharmaceutical Biotechnology (PANPB), National Research UniversityKhon Kaen University, Khon Kaen 40002, Thailand. Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand. Faculty of Pharmaceutical Sciences, Ubon Ratchathani University, Thailand. Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka 812–8582, Japan Madecassoside is the major triterpenoid saponins derived from Centella asiatica (l.) Urban, a medicinal plant used in Ayurvedic medicine for centuries. Madecassoside possesses various biological activities, including antidepressant effect (1), burn wound healing and anti-inflammatory (2). in this study, we developed the immunoassay method for quantitative analysis of the major compounds in C. asiatica. A sensitive indirect competitive enzyme-linked immunosorbent assay (EliSA) was developed using polyclonal antibody against madecassoside. Madecassoside-bovine serum albumin conjugate was immunized to rabbits for producing polyclonal antibody. The results showed that the antibodies were specific for madecassoside and aisaticoside. The range of the assay extends from 0.39 to 50 μg/ml. A good correlation between EliSA and hPlC methods was obtained when analysis of total triterpenoid saponins in the crude extracts of plant samples. in addition, the products containing C. asiatica in various preparations were determined triterpenoid saponins content by competitive EliSA. The developed EliSA method can be used for quality assessment of C. asiatica and their products.

Comparison of AMP579 and adenosine in inhibition of cell-cell interaction between human neutrophil and vascular endothelial cell

The purpose of the present study was to compare inhibitory effects between AMP579 (a new adenosine analog) and adenosine (Ado) in attenuating an interaction between human neutrophils (PMNs) and cultured human umbilical vein endothelial cells (HUVECs). PMN activation was determined by superoxide anion (O2–) production and degranulation (myeloperoxidase release). Cell–cell interaction was quantitated by adherence of fluorescent labeled PMNs to HUVECs. AMP579 inhibited O2– (nM/5 × 106 PMN) from fMLP-activated human PMNs (55.3 ± 3.1) in a concentration-dependent manner ranging from 31.1 ± 2.9 at 10 nM to 11.7 ± 0.9 at 10 ± μM, all P < 0.01 vs. fMLP group. In the same dose range, however, Ado showed significant inhibition only at 1 μM (30.3 ± 4.1) and 10 ± μM (27.5 ± 4.3) vs. the fMLP group. The calculated IC50 value (0.11 ± 0.05 μM) in AMP579 group was significantly less than that in the Ado group (4.1 ± 1.2 μM). Although there was no group difference on PMN myeloperoxidase release (percent inhibition from fMLP) between AMP579 and Ado at concentrations greater than 1 μM (52.9 ± 5.2 vs. 46.4 ± 5.9), AMP579 showed significant attenuation of degranulation compared with Ado at 10 nM (31.7 ± 2.5 vs. 11.6 ± 1.9) and 100 nM (48.2 ± 4.6 vs. 25.6 ± 3.8), respectively, suggesting that AMP579 is more potent in inhibiting PMN activation. AMP579 reduced PMN adherence to TNFα-stimulated HUVEC (fluorescent units/well) in a concentration-dependent manner from 472 ± 32 at 10 nM to 214 ± 15 at 10 μM vs. 675 ± 54 in the TNFα group. At 10 nM and 100 nM, adenosine did not attenuate PMN adherence, while it showed significant inhibition at 1 (504 ± 45) and 10 μM (435 ± 50), respectively. The IC50 value (2.8 ± 1 μM) for AMP579 was significantly lower than that (41 ± 8 μM) in the Ado group. The results from the present study suggest that 1) AMP579 directly inhibits adherence-independent superoxide radical generation and degranulation from activated PMNs and attenuates cell–cell interaction between PMNs and vascular endothelial cells by preventing damage on endothelial cells. 2) AMP579 exerts more potent protective effect compared with adenosine at a lower dose range, indicating its prospect for clinical application. Drug Dev. Res. 49:266–272, 2000. © 2000 Wiley-Liss, Inc.