Abstract
s. PHYTOPHARM 2012 M 90 Obzory po kliniceskoj farmacologii i lekarstvennoj terapii [Reviews of clinical pharmacology and drug therapy] ТОМ 10/2012/2 of 250mg/ml showed a potent inhibition effect toward platelet aggregation. Compared with other extracts, water extract exhibited the strongest inhibition as well as adenosine (50uM/ml) as a positive control. in Addition to this, methanol extracts showed relatively similar inhibition pattern with adenosine. finally, this initial finding has provided the evidence of Phyllantus niruri l. action in platelet functions. Table. Platelet Aggregation inhibition Adenosine Water Extract hexane Extract Chloroform Extract Ethanol Extract Methanol Extract inhibition ( %) 68.6 ± 4.3 72.7 ± 3.3 40.1 ± 5.6 44.7 ± 2.7 43.5 ± 6.6 68.8 ± 6.7 Values are mean ± SE (n = 8). Platelet counts was 1.5x108 cells/ml PRODUCTION OF ANTI–MADECASSOSIDE POLYCLONAL ANTIBODY AND ITS APPLICATION FOR QUALITY CONTROL OF CENTELLA ASIATICA (L.) URBAN © PutalunWaraporn , Tassanawat Patcharin , Yusakul Gorawit , Sritularak Boonchoo , Juengwatanatrakul Thaweesak , Tanaka Hiroyuki 5 Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, 40002, Thailand. Research Group for Pharmaceutical Activities of Natural Products using Pharmaceutical Biotechnology (PANPB), National Research UniversityKhon Kaen University, Khon Kaen 40002, Thailand. Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand. Faculty of Pharmaceutical Sciences, Ubon Ratchathani University, Thailand. Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka 812–8582, Japan Madecassoside is the major triterpenoid saponins derived from Centella asiatica (l.) Urban, a medicinal plant used in Ayurvedic medicine for centuries. Madecassoside possesses various biological activities, including antidepressant effect (1), burn wound healing and anti-inflammatory (2). in this study, we developed the immunoassay method for quantitative analysis of the major compounds in C. asiatica. A sensitive indirect competitive enzyme-linked immunosorbent assay (EliSA) was developed using polyclonal antibody against madecassoside. Madecassoside-bovine serum albumin conjugate was immunized to rabbits for producing polyclonal antibody. The results showed that the antibodies were specific for madecassoside and aisaticoside. The range of the assay extends from 0.39 to 50 μg/ml. A good correlation between EliSA and hPlC methods was obtained when analysis of total triterpenoid saponins in the crude extracts of plant samples. in addition, the products containing C. asiatica in various preparations were determined triterpenoid saponins content by competitive EliSA. The developed EliSA method can be used for quality assessment of C. asiatica and their products.
Highlights
Phyllanthus niruri L. is one of tropical plants that have been widely used in many countries as a traditional remedy for treating haemorrhagic diseases, in the case of low platelet count and disturbance of platelet functions
Blue pea extract was prepared by extraction of the petal of blue pea (Clitoria ternatea L.) in hot water for 2 hours and evaporate to 1 gram per 1 milliliter concentration
Blue pea extract gel was formulated with 32 % w/v blue pea extract, 2 % hydroxypropylmethylcellulose, 30 % of ethyl alcohol 0.5 % Bronidox 0.17 % triethanolamine and purify water to 100 ml
Summary
Phyllanthus niruri L. is one of tropical plants that have been widely used in many countries as a traditional remedy for treating haemorrhagic diseases, in the case of low platelet count and disturbance of platelet functions. The major objective of this study was the stability blue pea extract gel and CRC extract gel and the adsorption distance of the gel in the gray hair. Blue pea extract gel was formulated with 32 % w/v blue pea extract, 2 % hydroxypropylmethylcellulose, 30 % of ethyl alcohol 0.5 % Bronidox 0.17 % triethanolamine and purify water to 100 ml.
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