Additional Functions Research Articles

Advances in Filament Structure of 3D Bioprinted Biodegradable Bone Repair Scaffolds.

Conventional bone repair scaffolds can no longer meet the high standards and requirements of clinical applications in terms of preparation process and service performance. Studies have shown that the diversity of filament structures of implantable scaffolds is closely related to their overall properties (mechanical properties, degradation properties, and biological properties). To better elucidate the characteristics and advantages of different filament structures, this paper retrieves and summarizes the state of the art in the filament structure of the three-dimensional (3D) bioprinted biodegradable bone repair scaffolds, mainly including single-layer structure, double-layer structure, hollow structure, core-shell structure and bionic structures. The eximious performance of the novel scaffolds was discussed from different aspects (material composition, ink configuration, printing parameters, etc.). Besides, the additional functions of the current bone repair scaffold, such as chondrogenesis, angiogenesis, anti-bacteria, and anti-tumor, were also concluded. Finally, the paper prospects the future material selection, structural design, functional development, and performance optimization of bone repair scaffolds.

Development of a multilingual online course with the language support for teaching physics in English

<span lang="EN-US">Distance education offers an alternative to traditional teaching methods, particularly online courses. During the pandemic, the role of information and communication technologies in the field of education has increased and is used to organize both synchronous and asynchronous learning. However, not all information and communication technology (ICT) tools and platforms provide the necessary functionality for developing online courses in the direction of language integration. This article discusses the issues of creating a multilingual online course of physics in English with the necessary language support and additional functions for the development of students’ academic language. The study used such methods as a survey, focus groups, analytical and descriptive methods, and empirical research. The key features of the developed course are the use of various types of content and methods for assessing both subject and language knowledge and skills. The presented survey results, data on summative assessment (SE), and trial testing show the effectiveness of the developed course modules. The conclusions and results described in the article will be useful to teachers of natural sciences in general education and private schools, especially those using international programs, deputy directors, as well as teachers who teach their subjects in a second or third language.</span>

Stylistic and syntactic functions of parceling (on the material of B.L. Vasilyev’s novella "The Dawns Here Are Quiet")

The article describes one of expressive syntax techniques, namely parcelled constructions, identified in B.L. Vasilyev’s novella "The Dawns Here Are Quiet". The stylistic and syntactic functions of parcelling are determined and analysed using structural-semantic and functional stylistic analysis. The article concludes that parcelling as a stylistic device is a universal phenomenon of a multifunctional nature. It facilitates the implementation of the author’s various pragmatic ideas. Besides the main stylistic function, i. e. the function of expressively highlighting a certain part of the utterance, parcelled sentences perform several additional functions in a literary text: figurative, characterological, emotional-emphatic, expressive-grammatical. It is revealed that the syntactic functions of parcels in the novella are diverse. In simple sentences, they can be both their main and secondary parts. In composite sentences, they function as the predicative part which is attached to the main utterance using a subordinating, coordinating, or asyndetic connection. The variety of stylistic and syntactic functions of parcelled constructions enabled B. Vasilyev to realistically convey the atmosphere of the pre-war and war periods. He managed to describe the protagonists’ characters and their actions in a true to life way, making the narrative sound natural.

Interconnect Stress Testing as a Tool for Assessment of Reliability of Modern PCBs

The of miniaturization can be seen in electronics for some time already. The pinout of active components grows enabling them to have additional functions. The trend in usage of System in a Package (SiP) solutions is also evident thus routing of the active and passive elements became also challenging. To overcome this challenge boards with High Density Interconnection (HDI) where not only through hole, but also different kinds of microvias e.g. blind, buried, stacked, staggered via are used. The standard procedure to test reliability of boards and assemblies is very resource-hungry especially in time and money, as the tests have to be carried out in climatic chambers which lasts for weeks, followed by additional destructive testing like cross-sections. On the other hand IST (Interconnect Stress Testing) is a quick test, that enables to have assessment of the used technology especially the quality of the through holes of all types. Although the coupons are destroyed during the test, it is not necessary to test the manufactured circuits. This saves both materials and time. The technology optimizations and the key findings will be presented within the paper.

Hydrophobic solution functions as a multifaceted mosquito repellent by enhancing chemical transfer, altering object tracking, and forming aversive memory

Developing a safe and potent repellent of mosquitoes applicable to human skins is an effective measure against the spread of mosquito-borne diseases. Recently, we have identified that hydrophobic solutions such as low viscosity polydimethylsiloxane (L-PDMS) spread on a human skin prevent mosquitoes from staying on and biting it. This is likely due to the ability of L-PDMS in wetting mosquito legs and exerting a capillary force from which the mosquitoes attempt to escape. Here we show three additional functions of L-PDMS that can contribute to repel Aedes albopictus, by combining physicochemical analysis and behavioral assays in both an arm cage and a virtual flight arena. First, L-PDMS, when mixed with topical repellents and applied on a human skin, enhances the effect of topical repellents in reducing mosquito bites by efficiently transferring them to mosquito legs upon contact. Second, L-PDMS applied to mosquito tarsi compromises visual object tracking during flight, exerting an influence outlasting the contact. Finally, L-PDMS applied to mosquito tarsi acts as an aversive reinforcer in associative learning, making mosquitoes avoid the conditioned odor. These results uncover a multifaceted potential of L-PDMS in altering a sequence of mosquito behaviors from biting a human skin, visual object tracking following takeoff, to the response to an odor linked with L-PDMS.

Uloga glikoproteina 96 u karcinomu dojke

Glycoprotein 96 (gp96) is a member of the heat shock protein 90 family, which is an ubiquitous family of molecular chaperones that are involved in the regulation of protein folding and other essential cellular activities. Residing in the lumen of the endoplasmic reticulum, gp96 plays a key role in maintaining protein homeostasis, from assemblage to degradation. However, exposure to stressful conditions that disturb cellular homeostasis may translocate gp96 to the cell surface, which implies its additional functions, such as the regulation of intracellular signalling, proliferation, and apoptosis, as well as the modulation of the immune response. Besides its roles under physiological conditions, gp96 is also included in different stages of oncogenesis. In this review, we summarised available data on the structure, physiological, and pathophysiological roles of gp96, particularly in breast cancer oncogenesis.

Utilizing reactive polysulfides flux Na2Sx for the synthesis of sulfide solid electrolytes for all-solid-state sodium batteries

Sulfide solid electrolytes are attractive for the development of all-solid-state sodium batteries owing to their high ionic conductivities and formability. While the performance related to ionic conductivity and additional functions is strongly influenced by composition and crystal polymorphs, the use of delicate and less stable sulfide starting materials limits their variability.In this study, we propose a universal and convenient synthesis method for sulfide solid electrolytes utilizing Na2Sx polysulfide flux as a stoichiometric reactant. These polysulfides mitigate synthetic constraints by eliminating the requirement for unstable sulfide starting materials. They efficiently react with raw materials, including single elements, under ambient pressure, without the necessity for a sealed reaction vessel.Na3BS3 glass, and Na2.88Sb0.88W0.12S4 solid electrolytes was prepared by using Na2Sx working as lowly volatile flux to prepare. Na2S and S immediately reacted with each other without sulfur vaporization, and stoichiometric Na2Sx liquid was formed at temperatures above 500 °C. The Na2Sx flux itself oxidized B, Sb, and W uniformly at moderate temperatures, and stoichiometric electrolytes were obtained in a one-step process without sealed vessels and a vacuum condition. In addition, Na3BS3 glass was produced by direct quenching at 700 °C. The obtained Na2.88Sb0.88W0.12S4 demonstrated the extremely high ionic conductivity of 125 mS cm−1 at 25 °C, due to the stoichiometric substitution of WS42−.

Configuration of a telerehabilitation system to deliver a comprehensive aphasia therapy program via telerehabilitation (TeleCHAT): A human-centred design approach

ABSTRACT The delivery of Intensive Comprehensive Aphasia Programs (ICAPs) via telerehabilitation is a potential solution to the ongoing geographical, transport, and mobility barriers that people with aphasia (PWA) face in accessing this service model. To ensure the ICAP retains its evidence-based elements, configuration of the telerehabilitation system requires consideration of user and therapy task requirements. The human-centred design (HCD) process incorporates user and task considerations when developing technological systems. Therefore, the aim of this study was to use the HCD process to translate the Comprehensive High-dose Aphasia Therapy (CHAT) program for delivery via telerehabilitation (TeleCHAT). Methods The HCD translation process used consisted of five iterative stages. Relevant literature was reviewed, and speech-language pathologists (SLPs) experienced in delivering ICAPs were consulted to understand the therapy context. Researchers gathered therapy task and user requirements of PWA, support people (SP) and SLPs to engage in therapy. Technology that met user and therapy task requirements were identified and a preliminary technology configuration was tested in a simulated environment. The finalised system was tested in a pilot trial of TeleCHAT with a cohort of four PWA, two SPs, and two SLPs. Feedback and technical and user issues were collated from users and analysed to inform improvements to the TeleCHAT configuration and protocol. Results User and therapy task requirements to deliver TeleCHAT were identified with consideration of communication and participation supports for PWA. The configured technology system comprised of Zoom© as the videoconferencing platform of choice, and additional critical technical functions including screen and audio sharing of therapy content, and multi-point interaction using remote control and annotation tools. Simulation testing and a pilot trial of the configuration confirmed system operability and identified modifications to optimise functioning. Conclusion The HCD framework was used to successfully configure an optimal technology system for the delivery of TeleCHAT. The HCD framework can be used by clinicians to identify patient needs and configure optimal telerehabilitation systems for delivery of complex interventions.

BECLIN1 is essential for intestinal homeostasis involving autophagy-independent mechanisms through its function in endocytic trafficking

Autophagy-related genes have been closely associated with intestinal homeostasis. BECLIN1 is a component of Class III phosphatidylinositol 3-kinase complexes that orchestrate autophagy initiation and endocytic trafficking. Here we show intestinal epithelium-specific BECLIN1 deletion in adult mice leads to rapid fatal enteritis with compromised gut barrier integrity, highlighting its intrinsic critical role in gut maintenance. BECLIN1-deficient intestinal epithelial cells exhibit extensive apoptosis, impaired autophagy, and stressed endoplasmic reticulum and mitochondria. Remaining absorptive enterocytes and secretory cells display morphological abnormalities. Deletion of the autophagy regulator, ATG7, fails to elicit similar effects, suggesting additional novel autophagy-independent functions of BECLIN1 distinct from ATG7. Indeed, organoids derived from BECLIN1 KO mice show E-CADHERIN mislocalisation associated with abnormalities in the endocytic trafficking pathway. This provides a mechanism linking endocytic trafficking mediated by BECLIN1 and loss of intestinal barrier integrity. Our findings establish an indispensable role of BECLIN1 in maintaining mammalian intestinal homeostasis and uncover its involvement in endocytic trafficking in this process. Hence, this study has important implications for our understanding of intestinal pathophysiology.

Introducing UCST onto Chitosan for a Simple and Effective Single-Phase Extraction.

Upper critical solution temperature (UCST) polymers undergo their own collapsed structures to show thermoresponsive functions favoring controlled release systems, cell adhesion, including separation process, etc. Although the copolymerization of UCST monomers with other vinyl monomers containing a pendant group is a good way to introduce additional functions, uncertain UCST performance as well as extensive bio-related properties are always the points to be considered. To accomplish this, the present work proposes the application of polysaccharides, i.e., chitosan (CS), as the biopolymer backbone to conjugate with functional molecules and UCST polymers. The use of chain transfer agents, e.g., mercaptoacetic acid, in radical polymerization with UCST poly(methacrylamide) (PMAAm) via the CS/NHS (N-hydroxysuccinimide) complex allows the simple water-based modification. The further conjugation of mouse anti-LipL32 IgG monoclonal antibody (anti-LipL32 mAb) onto CS-PMAAm (CS-PMAAm-Ab) enables a selective binding of recombinant LipL32 (rLipL32) antigen (Ag) in the solution. The CS-PMAAm obtained not only shows the cloud point in the range of 10-30 °C but also the extraction of rLipL32 because of CS-PMAAm-Ab-Ag aggregation. The present work demonstrates how CS expresses UCST with additional antibody conjugated is feasible for a simple and effective Ag single-phase extraction.

Comparison of Descriptive Assessment and Trial-Based Functional Analysis as the Basis of Treatment in Homes

Effective treatment of challenging behaviors in young children with autism spectrum disorder requires identifying consequences that maintain the behavior under specific environmental circumstances. Assessment strategies help to identify environmental factors setting the occasion for the behaviors to continue to occur. Many studies have used antecedent–behavior–consequence (ABC) data collection strategies, but few have examined its ability to effectively identify the function. Research supports the use of the trial-based functional analysis (TBFA) but few studies examine its effectiveness in the home setting. The current study attempted to answer which assessments might work best in the home setting. Treatment was implemented for each participant based on the results of their assessments. All assessments identified the function of behavior as verified by function-based treatments, but the ABC assessment identified additional functions for three of the participants while the TBFA was more precise. Implications and limitations are reviewed, along with future directions for behavioral assessment in the home.

Tailoring the Function of Battery Separators via the Design of MOF Coatings

AbstractSeparators serve as critical components in batteries, bearing the responsibility for battery safety. In addition to the demand for increased flame resistance of separators, there is a growing interest in the additional functions of battery separators. In this work, a multifunctional coating integration strategy is proposed for battery separators via the versatility of metal–organic frameworks. A versatile and high‐safety MIP‐202@2320 composite separator is engineered and it is integrated into lithium–sulfur batteries. The presence of Cl− and the porous structure of MIP‐202 impart flame‐retardant characteristics and high electrolyte retention (80%) to resulting separators. Moreover, coupled with the negatively charged electron cloud formed by Cl− ions within the channels, it can selectively impede the polysulfides from migrating toward the anode, while concurrently facilitating the movement of Li+ ions. The assembled lithium–sulfur battery with MIP‐202@2320 as the separator maintains an impressive 71.8% capacity retention even after 600 cycles at a 0.5C rate. This multifunctional integration strategy not only boosts the performance of lithium–sulfur batteries but also pioneers a new direction for the design of future battery separators for different kinds of batteries.

On the set of reduced states of translation invariant, infinite quantum systems

The set of two-body reduced states of translation invariant, infinite quantum spin chains can be approximated from inside and outside using matrix product states and marginals of finite systems, respectively. These lead to hierarchies of algebraic approximations that become tight only in the limit of infinitely many auxiliary variables. We show that this is necessarily so for any algebraic ansatz by proving that the set of reduced states is not semialgebraic. We also provide evidence that additional elementary transcendental functions cannot lead to a finitary description.

Multifunctional hydrogels for wound healing

Abstract The process of wound healing is complex and dynamic. Given the unique nature of the skin, wound healing requires dressings that meet a wide range of functional requirements. Due to their unique structure and properties, hydrogel dressings provide ideal conditions for supporting the process of wound healing and can be enhanced with additional functions to meet the specific requirements of different types of wounds. Compared to traditional wound dressings, the newly developed hydrogel wound dressing offers several advantages, including its soft texture, ability to retain moisture, oxygen permeability, and comfort. With the advancement of medical technology, higher demands are being placed on the functionality of hydrogel dressings. Therefore, this paper offers a comprehensive overview of hydrogel wound dressings with various functions. These functions primarily include hemostatic hydrogel dressings, antibacterial hydrogel dressings, angiogenesis-promoting hydrogel dressings, and other functional hydrogel dressings. The paper also reviews the research and application of these dressings in recent years.

A Comprehensive Review of Therapeutic Compounds from Plants for Neurodegenerative Diseases.

Neurodegenerative diseases (NDDs) comprise a large number of disorders that affect the structure and functions of the nervous system. The major cause of various neurodegenerative diseases includes protein aggregation, oxidative stress and inflammation. Over the last decade, there has been a gradual inclination of neurological research in order to find drugs that can prevent, slow down, or treat these diseases. The most common NDDs are Alzheimer's, Parkinson's, and Huntington's illnesses which claims the lives of 6.8 million people worldwide each year and it is expected to rise by 7.1%. The focus on alternative medicine, particularly plant-based products, has grown significantly in recent years. Plants are considered a good source of biologically active molecules and hence phytochemical screening of plants will pave the way for discovering new drugs. Neurodegeneration has long been linked to oxidative stress, either as a direct cause or as a side effect of other variables. Therefore, it has been proposed that the use of antioxidants to combat cellular oxidative stress within the nervous system may be a viable therapeutic strategy for neurological illnesses. In order to prevent and treat NDDs, this review article covers the therapeutic compounds/ metabolites from plants with the neuroprotective role. However, these exhibit other beneficial molecular functions in addition to antioxidant activity is the potential application in the management or prevention of neurodegenerative disorders. Further, it gives future researchers the significance of considering peptide-based therapeutics through various mechanisms in delaying or curing neurodegenerative diseases.

Protein Glycosylation Patterns Shaped By the IRE1‐XBP1s Arm of the Unfolded Protein Response

AbstractThe unfolded protein response (UPR) is a sensing and signaling pathway that surveys the endoplasmic reticulum (ER) for protein folding challenges and responds whenever issues are detected. UPR activation leads to upregulation of secretory pathway chaperones and quality control factors, as well as reduces the nascent protein load on the ER, thereby restoring and maintaining proteostasis. This paradigm‐defining view of the role of the UPR is accurate, but it elides additional key functions of the UPR in cell biology. In particular, recent work has revealed that the UPR can shape the structure and function of N‐ and O‐glycans installed on ER client proteins. This crosstalk between the UPR's reaction to protein misfolding and the regulation of glycosylation remains insufficiently understood. Still, emerging evidence makes it clear that the UPR, and particularly the IRE1‐XBP1s arm of the UPR, may be a central regulator of protein glycosylation, with important biological consequences. In this review, we discuss the crosstalk between proteostasis, the UPR, and glycosylation, present progress towards understanding biological functions of this crosstalk, and examine potential roles in diseases such as cancer.

К ВОПРОСУ О НАДЕЛЕНИИ СПЕЦИАЛИЗИРОВАННЫХ ПОДРАЗДЕЛЕНИЙ ПО ОБЕСПЕЧЕНИЮ ОБЩЕСТВЕННОГО ПОРЯДКА (в перевозочном и технологическом секторах объектов транспортной инфраструктуры) ОРГАНОВ ВНУТРЕННИХ ДЕЛ РОССИЙСКОЙ ФЕДЕРАЦИИ НА ТРАНСПОРТЕ ДОПОЛНИТЕЛЬНЫМИ ФУНКЦИЯМИ, ЗАДАЧАМИ И ПОЛНОМОЧИЯМИ

The article discusses issues of legal support for transport security at transport infrastructure facilities. Taking into account the forecast indicators for the development of transport facilities, a reasonable conclusion is given about the need to improve legislation in the field of transport security. It is noted that the system of counteracting acts of illegal interference in transport activities will be most effective with close interaction between the internal affairs bodies of the Russian Federation in transport and the forces ensuring transport security at transport infrastructure facilities. Based on the analysis, proposals have been devel-oped to provide specialized units of the internal affairs bodies of the Russian Federation in transport with additional functions, tasks and powers.

Chemical Proteomics Reveals Nε-Fatty-Acylation of Septins by Rho Inactivation Domain (RID) of the Vibrio MARTX Toxin to Alter Septin Localization and Organization

Vibrio species, the Gram-negative bacterial pathogens causing cholera and sepsis, produce multiple secreted virulence factors for infection and pathogenesis. Among these is the multifunctional-autoprocessing repeats-in-toxin (MARTX) toxin that releases several critical effector domains with distinct functions inside eukaryotic host cells. One such effector domain, the Rho inactivation domain (RID), has been discovered to catalyze long-chain Nε-fatty-acylation on lysine residues of Rho GTPases, causing inactivation of Rho GTPases and disruption of the host actin cytoskeleton. However, whether RID modifies other host proteins to exert additional functions remains to be determined. Herein, we describe the integration of bioorthogonal chemical labeling and quantitative proteomics to globally profile the target proteins modified by RID in living cells. More than 246 proteins are identified as new RID substrates, including many involved in GTPase regulation, cytoskeletal organization, and cell division. We demonstrate that RID extensively Nε-fatty-acylates septin proteins, the fourth cytoskeletal component of mammalian cells with important roles in diverse cellular processes. While affinity purification and mass spectrometry analysis show that RID-mediated Nε-fatty-acylation does not affect septin-septin interactions, this modification increases the membrane association of septins and confers localization to detergent-resistant membrane rafts. As a result, the filamentous assembly and organization of septins are disrupted by RID-mediated Nε-fatty-acylation, further contributing to cytoskeletal and mitotic defects that phenocopy the effects of septin depletion. Overall, our work greatly expands the substrate scope and function of RID and demonstrates the role of RID-mediated Nε-fatty-acylation in manipulating septin localization and organization.

Arabidopsis leucine-rich repeat malectin receptor-like kinases regulate pollen-stigma interactions.

Flowering plants contain tightly controlled pollen-pistil interactions required for promoting intraspecific fertilization and preventing interspecific hybridizations. In Arabidopsis (Arabidopsis thaliana), several receptor kinases (RKs) are known to regulate the later stages of intraspecific pollen tube growth and ovular reception in the pistil, but less is known about RK regulation of the earlier stages. The Arabidopsis RECEPTOR-LIKE KINASE IN FLOWERS1 (RKF1)/RKF1-LIKE (RKFL) 1-3 cluster of 4 leucine-rich repeat malectin (LRR-MAL) RKs was previously found to function in the stigma to promote intraspecific pollen hydration. In this study, we tested additional combinations of up to 7 Arabidopsis LRR-MAL RK knockout mutants, including RKF1, RKFL1-3, LysM RLK1-INTERACTING KINASE1, REMORIN-INTERACTING RECEPTOR1, and NEMATODE-INDUCED LRR-RLK2. These LRR-MAL RKs were discovered to function in the female stigma to support intraspecific Arabidopsis pollen tube growth and to establish a prezygotic interspecific barrier against Capsella rubella pollen. Thus, this study uncovered additional biological functions for this poorly understood group of RKs in regulating the early stages of Arabidopsis sexual reproduction.

Introducing QAnnAGNPS - A QGIS plugin to facilitate the use of AnnAGNPS (Annualized Agricultural Nonpoint source model)

AnnAGNPS is a watershed-scale hydrological model designed to analyze the impact of nonpoint source pollution in agricultural environments. Its unique capabilities have defined it as an essential model that is used globally to evaluate agricultural management scenarios. However, it does not currently have a user-friendly graphical interface that provides a simple way for users to perform simulations. This article presents QAnnAGNPS, a plugin developed in QGIS to facilitate access to the simulation capabilities of AnnAGNPS through a user-friendly interface and the addition of extra features, including data visualization. QAnnAGNPS, in addition to fulfilling this valuable task, opens the door to the incorporation of additional functions already included in other similar hydrological models. The plugin has been tested in the Latxaga cereal basin in Navarra, Spain, and has demonstrated that it provides a simpler way to perform simulations and visualize results compared to AnnAGNPS.