Addition Of Fertilizer Research Articles

Evaluation of dimethandrolone undecanoate in non-human primates as a candidate for long-acting injectable male contraceptive.

Dimethandrolone undecanoate (DMAU) is under development as a single agent hormonal male contraceptive. DMAU is a prodrug hydrolyzed by esterase(s) to the active metabolite dimethandrolone (DMA) which has dual androgenic and progestogenic actions. Phase 1 clinical trial results show DMAU to be well-tolerated as an oral contraceptive in healthy men; however, delivery of DMAU as a long-acting injectable rather than a daily oral formulation would provide user compliance benefits and address oral bioavailability concerns. To assess the safety, pharmacokinetics (PK), and long-acting contraceptive potential of DMAU in male non-human primates (NHP) when delivered as an injectable or oral formulation. DMAU was administered to cynomolgus macaques orally for 9 months or as five weekly intramuscular (IM) injections and to rhesus macaques as a single IM injection. Evaluations of safety, fertility indicators, and serum levels of DMAU and DMA were followed>2 years post-dose. Repeat dose oral and IM administrations were well-tolerated with no significant toxicological findings. Dramatic reductions in serum testosterone concentration occurred within days of administration followed by sustained suppression of additional fertility indicators (e.g., serum inhibin B, sperm count, and testicular spermatogenesis). Slight body weight increases and reductions in testes weight also occurred. Repeat DMAU injections resulted in DMA serum concentrations above the lower limit of quantification (1ng/mL) for>500 days. DMAU PK parameters increased with increasing IM dose, while dose dependence was not seen for serum DMA concentrations suggesting a depot effect with a reservoir of non-circulating prodrug remaining at the injection site which gets slowly hydrolyzed to DMA and absorbed into circulation. Testosterone levels and spermatogenesis returned by the end of the recovery period. Nonclinical safety, PK, and pharmacodynamic data in male NHP demonstrate the safe, well-tolerated, long-acting, and reversible contraceptive potential of injectable DMAU.

A 2-year pure biochar addition enhances soil carbon sequestration and reduces aggregate stability in understory conditions

The enhancement of soil aggregate size and stability is crucial for mitigating climate change and improving carbon sequestration in forest ecosystems. Biochar, derived from rice husks, has been suggested as an effective mean to increase soil carbon storage. However, isolating biochar’s specific effects on soil aggregate formation and carbon sink capacity can be complex due to the overlapping influences of fertilization and understory vegetation cultivation. Our study circumvented these variables by incorporating different amounts of biochar into plantation soil without any additional cultivation or fertilization, conducting a detailed two–year field experiment. The findings revealed that biochar significantly increased the organic carbon content and density in the uncultivated under–forest Ferralsols, thus enhancing its carbon sink function. Intriguingly, while biochar raised the proportion of small soil aggregates (< 0.25 mm) and their organic carbon levels, it decreased the fraction of larger aggregates (> 0.25 mm), adversely affecting soil aggregate stability. These results suggest that biochar may compromise soil aggregate structure and stability in the absence of plant growth. The positive impact of biochar on soil carbon storage was found to depend more on its inherent inert carbon content than on soil type. Moreover, biochar alone was insufficient to increase the quantity of soil macroaggregates without the binding action of plant root exudates. Biochar’s key function appears to be in enhancing the soil aggregate–forming processes facilitated by plant roots and microorganisms. Therefore, for optimal carbon sequestration in forest soils, integrating biochar application with appropriate agricultural practices is advisable.

The Influence of Sowing Rate and Foliar Fertilization on the Yield of Some Triticale Varieties in the Context of Climate Change in Northwest Romania

Triticale is recognized worldwide because of its high protein and lysine contents, high production capacity, and adaptability to biotic and abiotic stress conditions, these qualities being taken over from wheat and rye. Triticale is widely used in various fields, such as animal feed in various forms, medicine, baking, beer and alcohol brewing, cellulose, bioethanol industry, and many others. Thus, the demand for triticale grain is increasing, and this has led to the research and improvement in culture technology to obtain superior products, both quantitatively and qualitatively. The purpose of this study was to identify the best varieties of triticale cultivated in the northwest of Romania, sown in different plots, and fertilized on the ground. Additionally, this study was carried out over a period of two years at the Livada Agricultural Research and Development Station, Satu Mare County. This study was located on acidic soil with a pH between 5.19 and 6.65 and a humus content of 2.82%. The climatic conditions in the reference period were extremely variable; in the first year, a deficit of more than 90 mm of precipitation was registered, and in the second year of this study, an increase of more than 34 mm. The effects of additional fertilization were influenced by the level of precipitation. In 2023, additional fertilization with foliar fertilizer brought production increases of 884 kg/ha, compared with 2022, where foliar fertilization in drought conditions led to a decrease in production. The Utrifun variety proved to be the most productive; this foliar fertilized and with biostimulator, sown at 550 seeds/m2, recorded an increase in production of over 4500 kg/ha compared with the Negoiu control and sown at 650 seeds/m2 and fertilized with foliar fertilizer had an increase of over 4370 kg/ha compared with the Negoiu control. It recorded a production of 9700 kg/ha sown at 550 seeds/m2 and fertilized only on the soil, and sown at 450 seeds/m2 and additionally fertilized with foliar fertilizer recorded a production of 10.900 kg/ha. Utrifun was followed by Zvelt which, sown at 450 seed/m2 and fertilized on the soil, recorded 9500 kg/ha, ensuring an increase of 1800 kg/ha compared with the Negoiu control. The lowest production was achieved by the Tulnic variety, which is 2022, sown at 650 seed/m2 and fertilized on the ground, recorded 5874 kg/ha, 1621 kg/ha less than the control variant. The increases in production obtained by these varieties will be confirmed in a subsequent study under different climatic conditions.

Reducing ammonia volatilization in rice paddy: the importance of lower fertilizer rates and soil incorporation

In rice paddies, which exhibit higher ammonia (NH₃) emission factors than upland soils, identifying key drivers of NH₃ flux intensity is crucial. Contrary to the commonly held view that NH₃ flux is primarily governed by soil ammonium (NH₄⁺) concentrations, we found no significant relationship between NH₃ flux and NH₄⁺ levels in the soil during rice cultivation. To pinpoint a primary factor influencing NH₃ flux intensity under conventional rice cropping practices, we conducted a 2-year field study applying four nitrogen (N) fertilization rates (0, 45, 90, and 180 kg N ha⁻¹) using urea [(NH₂)₂CO], the most common N fertilizer. NH₃ emissions were tracked using the ventilation method. Following N application, NH₃ flux sharply increased but rapidly returned to baseline. Half of the N applied as a basal fertilizer was incorporated within the soil, contributing only 10% of total NH₃ emissions. In contrast, top-dressed applications—20% of total N at the tillering stage and 30% at panicle initiation—accounted for approximately 90% of NH₃ loss. Seasonal NH₃ flux increased quadratically with rising N application rates, correlating strongly with NH₄⁺ concentrations in floodwater rather than soil. Grain yield responded quadratically to N levels, peaking at 120 kg N ha⁻¹ with a 37% increase over control yields. NH₃ flux intensity, defined as seasonal NH₃ flux per unit of grain yield, showed a quadratic response to N fertilization, decreasing with initial fertilizer additions (up to 38 kg N ha⁻¹) but then sharply increased with further N fertilization increase. Hence, reducing NH₄⁺ concentrations in floodwater through moderated N application and deeper fertilizer placement could be essential for minimizing NH₃ volatilization in rice systems.

Organic Amendment Enhances Maize Yield Through Improved Photosynthesis, Endogenous Hormones, and Defense Enzymes

Enhancing photosynthesis is considered one of the most rational strategies to increase crop yield. However, the dominant physiological driving processes of photosynthesis at different maize growth stages under long-term fertilization remain unclear. Based on a 45-years field experiment, four treatments were selected—namely, CK (no fertilizers), N (276 kg N ha−1), M (60,000 kg manure ha−1), and MN (60,000 kg manure + 276 kg N ha−1)—to study the response of maize physiological characteristics to fertilization. The results showed that long-term fertilization significantly changed the photosynthetic characteristics and endogenous hormones content. Among them, MN increased the photosynthetic rate (Pn) by 41.9–152.3% at different maize growth stages compared to CK. Organic fertilizer addition (M and MN) significantly increased superoxide dismutase (SOD), peroxidase (POD), and soluble sugar (SS) by 26.7–64.3%, 42.5–155.4%, and 16.4–89.5%, respectively. However, proline (Pro) and malondialdehyde (MDA) content decreased by 19.1–44.3% and 0.6-21.3%, respectively. Under these treatments, indole-3-acetic acid (IAA), gibberellins (GA), salicylic acid (SA), zeatin riboside (ZR), abscisic acid (ABA), SS, SOD, and POD were the main physiological driving indicators of photosynthesis. The result of PLS-SEM indicated that the main physiological driving pathways of photosynthesis were “IAA or ZR or SA-SOD-POD-Pn” and “GA or ABA-SOD or POD-SS-Pn”. Additionally, the maize grain yield of N, M, and MN increased by 111.2%, 218.1%, and 297.2% compared to CK, respectively. Overall, organic combined with nitrogen fertilizer could maintain a higher level of photosynthetic capacity by balancing endogenous hormones and enhancing the activity of antioxidant enzymes to support maize production.

Sample preparation method for IR analysis of petroleum-contaminated soil: An innovative technology for ecological remediation using Miscanthus x giganteus

Miscanthus x giganteus phytoremediation of soil contaminated with petroleum was assessed in this study. A method of soil sample preparation for determining the total content of petroleum products by infrared spectrophotometry has been developed. It is a one-stage extraction method with minimal use of carbon tetrachloride as an extractant. This soil sample preparation method was environmentally friendly and cost-effective, as it required a significantly lower amount of extractant (15–30 ml of tetrachloromethane) compared to the commonly used threefold extraction method, which uses up to 150 ml of extractant. The extraction degree of petroleum products (PP) was determined to be from 81.78 % to 94.22 % after two days of extraction using the additive method of determining PP. It was observed that the presence of different fertilizer additives in the soil samples led to a reduction in the determined PP content in the following series: “without fertilizer” – “Biochar” additive – “Biohumus” additive. These results were compared with reference samples that did not involve the use of Miscanthus x giganteus. Furthermore, the main thermolysis stages of petroleum products sorbed by the soil matrix and the thermal behavior of an artificial soil sample spiked with PP were examined. Detailed interpretation of thermograms of laboratory soil samples was conducted at various phytoremediation stages.

Transcriptome Responses of the Soil-Dwelling Collembolan (Entomobrya proxima Folsom) to Fertilizer Type and Concentration.

Soil collembolans have been regarded as the effective bioindicator of environmental changes. However, the physiological mechanisms through which collembolans respond to agricultural activities are largely unknown. Given the plasticity and sensitivity to environmental changes, even subtle responses can be quantified via transcriptomics. Therefore, the relevant in situ soil ecosystem and numerically dominant collembolan species Entomobrya proxima Folsom was selected to explore the dynamic responses to fertilizer type and concentration using transcriptome sequencing over three periods (6 h, 24 h and 10 d). The results showed that exposure duration caused significant alterations in gene expression profiles. At day 10 after exposure, gene expression patterns differed remarkably between the two fertilizer types and the control. Relative to organic fertilizer, the number of DEGs was increased by 114.31% under inorganic fertilizer, which declined with increasing inorganic fertilizer concentrations. Functional enrichment analysis was indicative of enhanced fatty acid and carbohydrate metabolism and reduced disease occurrence by organic fertilizer; however, an inhibited lipid synthesis process promoted susceptibility to infection, triggered oxidative stress, etc. by inorganic fertilizer. Overall, fertilizer addition changed the transcriptional pattern of the collembolan, potentially causing shifts in pathways related to metabolism, immunity, etc. In comparison to inorganic fertilizer, organic fertilizer impacted less on the gene expression patterns, implying that organic fertilizer application may be more beneficial to soil animal health.

Site-specific effects of fertilizer on hay and grain yields of oats: evidence from large-scale field experiments.

Oat (Avena sativa L.) is a valuable crop due to its strong adaptability to marginal environments, making it an important component of agricultural systems in regions where other cereals may not thrive. The application of chemical fertilizer can influence oat hay and grain yield significantly. However, large-scale meta-analytical studies of the size and variability of oat hay and grain yields in response to fertilizer addition are still lacking. Based on 83 studies worldwide, this meta-analysis quantifies the impact of the addition of fertilizer on oat hay and grain yields under varying environmental conditions (e.g., soil nutrient levels, texture, and climate). The results confirmed that the fertilizer application increased oat hay yield by 48.9% and grain yield by 36.2%. This study demonstrated that balanced fertilization with nitrogen, phosphorus, and potassium generally enhances oat hay and grain yield despite large temporal and spatial variations. Boosted regression tree (BRT) models suggest that changes in hay and grain yield were primarily dominated by soil pH and nitrogen fertilizer. The response ratio (the natural logarithm of the mean values of hay yield or grain yield with and without fertilization, respectively) of hay yield declined linearly with soil pH. Elevation was the second most important factor affecting the change in response ratio of hay yield and the third most important factor affecting the change in response ratio of grain yield but climatic conditions were not the dominant factors affecting changes in oat hay or grain yield. Overall, these results will benefit producers considering site-specific fertilization management of oat. They could increase yields and save investment in fertilizer, and help to facilitate the genetic breeding of oat varieties with high nutrient use efficiency. © 2024 The Author(s). Journal of the Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

Effect of Synthetic Fertilizer on Diazotrophic Bacteria in Secondary Forest and Oil Palm Soils in Central Kalimantan

Soil microorganisms, especially diazotrophic bacteria, are vital for ecosystem sustainability, significantly contributing to nitrogen cycling and biodiversity conservation. Understanding the impact of different land-use practices on soil microorganisms, especially synthetic fertilizer addition, is essential for sustainable agriculture. This study compares soil properties, bacterial densities, and responses to fertilization in secondary forest soils and adjacent oil palm plantation soils located in PT Kerry Sawit Indonesia, Central Kalimantan. A greenhouse experiment was conducted using both secondary forest and oil palm plantation soils to evaluate the impacts of different synthetic fertilizers on nitrogen-fixing bacteria and soybean agronomic performance. Total soil bacteria and diazotrophic bacteria, essential for nitrogen fixation, were analyzed through microbiological assays and qPCR focusing on the nifH gene. Our findings show that oil palm plantation soils had higher levels of nitrogen, phosphate, and nitrate, while secondary forest soils had a higher abundance of diazotrophic bacteria. Furthermore, excessive nitrogen fertilization was found to reduce microbial density, negatively impacting plant sustainability, highlighting the importance of customized fertilizer management. The study highlights the intricate connections between land-use practices and microbial populations, providing valuable insights for balancing agricultural productivity with ecological sustainability.

Short-term organic fertilizer substitution increases sorghum yield by improving soil physicochemical characteristics and regulating microbial community structure.

Chemical fertilizer reduction combined with organic fertilizer (organic fertilizer substitution) has a positive impact on crop productivity and sustainable development. However, the effects of short-term organic fertilizer substitution on microbial community structure and functions of sorghum rhizosphere soil and on sorghum yield remain unclear. Herein, this study investigated the short-term effects of organic fertilizer substitution on sorghum soil physicochemical properties, microbial community structure and enzyme activities using Metagenomic sequencing technology. The fertilization treatment included no fertilization (CK), pure chemical fertilizer N (CF), substitution of 25% chemical fertilizer N with organic N (NF25), substitution of 50% chemical fertilizer N with organic N (NF50), substitution of 75% chemical fertilizer N with organic N (NF75), and pure organic fertilizer N (NF100); soil samples were collected and analyzed in the flowering period of sorghum. The results showed that the suitable organic fertilizer substitution rate of sorghum field was 50%, and its yield was the highest among all treatments (9789.9 kg/hm2). Compared with the CF treatment, a medium ratio (50%) of organic fertilizer substitution significantly reduced soil alkalization (by 3.05%), improved soil nutrients, enhanced soil enzyme activities, and increased sorghum yield (P < 0.05). After organic fertilizer substitution treatment, higher protein, fat, and total starch levels accumulated in sorghum grains, and the tannin content of grains decreased. The effect of organic fertilizer substitution on bacterial diversity was greater than that on fungal diversity. Among the dominant bacterial phyla, the medium ratio of organic substitution treatment significantly increased the relative abundances of Proteobacteria (by 3.57%) and Actinomycetes (by 14.94%), and decreased the relative abundances of Acidobacteria (by 5.18%) and Planctomycetes (by 7.76%) compared with no fertilization, while the dominant fungal phyla did not respond significantly to the addition of organic fertilizer. Organic fertilizer substitution also improved soil microbial metabolic pathways, biosynthesis of secondary metabolites, and carbon metabolism. The biomarkers enriched in inorganic fertilizer treatment and organic fertilizer substitution treatments had similar relevant environmental elements but reversed correlation trends. Moreover, soil Alkali-hydrolyzable nitrogen and L-leucine aminopeptidase were important environmental factors influencing the structure of bacterial and fungal communities in sorghum soils, respectively. Soil nutrient levels and microbial communities together explained the variation in annual sorghum yield. The results of this study provide evidence that short-term organic fertilizer substitution increases sorghum yield by improving soil properties and regulating microbial community structure.

Effects of exogenous nitrogen addition on soil organic nitrogen fractions in different fertility soils: Result from a 15N cross-labeling experiment

Exogenous nitrogen (N) addition serves as a pivotal nutrient management strategy, significantly enhancing agricultural production by regulating soil N availability and retention. However, the dynamics of soil organic nitrogen (SON) fractions in response to various forms of exogenous N addition across differing soil fertility levels remain inadequately understood. This study utilized data from a 25-year fertilization experiment and a 15N cross-labeling experiment in Northeast China to assess and quantify the effects of mineral N fertilizers and organic materials (manure and straw) on SON fractions in NPK (mineral fertilizer addition) and NPKM (NPK combined with composted pig manure) treatments. Our findings indicate that long-term incorporation of manure substantially elevates soil fertility compared to the exclusive use of mineral fertilizers. Notably, exogenous N primarily boosts soil N availability by enhancing acid-soluble organic N fractions, particularly ammonium nitrogen (AN) and amino acid nitrogen (AAN). Organic materials, particularly straw, significantly enhanced the retention of mineral fertilizer N in both NPK and NPKM treatments (9.54 % vs 10.70 %). Moreover, over 70 % of the N from straw or manure remained in the soil as stable SON fractions. While straw rapidly improves low-fertility soils, manure contributes to enhanced soil N reserves and increased crop yields. Therefore, incorporating organic matter may bolster soil N sequestration in Northeast China, which is contingent upon soil fertility and tailored fertilizer management strategies. This research elucidates the distribution and conversion of exogenous N within SON pools, facilitating optimized N management, sustaining yields, reducing farmland N pollution, and promoting agricultural sustainability.

Biophysical and socioeconomic characterization of Agewmariam experimental watershed in Northern Ethiopia: insights and management options.

Watershed characterization is essential for sustainable watershed management and effective resource utilization, particularly in assessing changes resulting from interventions. This study investigates the biophysical and socio-economic conditions of the Agewmariam watershed, aiming to develop land capability and suitability maps while identifying viable management options. Biophysical data, including land slope, soil properties, erosion severity, stoniness/rockiness cover, and vegetation cover, were collected through field surveys and analyzed using overlay analysis in ArcGIS. Socio-economic data, encompassing population demographics, income, and expenditure, were gathered via individual interviews, complete census, and focus group discussions and summarized using descriptive statistics. The study identified 259 households with an average family size of 4 and a demographic dependency ratio of 96.6%. The major sources of income are crop production and livestock rearing supplemented by casual labor and food aid. The average farmland holding size is 0.5ha, with sorghum, barley, teff, and wheat as the dominant crops. The watershed is characterized by six land capability classes (classes II, III, IV, VI, VII, and VIII). The suitability analysis indicated that the watershed is not currently suitable for wheat and teff crops unless physical, chemical, and biological management improvements are implemented. The study revealed that the major limiting factors for land capability and suitability were slope, erosion severity, stoniness, soil organic matter, and soil texture. The socio-economic characterization enhanced awareness about the local socio-economic condition, informing appropriate planning and management strategies. The study recommends intensive soil and water conservation intervention, afforestation on hillsides, changing the land use system, and the addition of organic matter and fertilizers to enhance land capability and suitability for sustainable agricultural practices.

Consequential life cycle assessment of urban source-separating sanitation systems complementing centralized wastewater treatment in Lund, Sweden

This study examined various source-separating sanitation systems to evaluate their environmental performance, providing decision-makers with insights for selecting an appropriate system for a newly developed neighborhood in Sweden. A full consequential LCA was conducted to account for resource recovery and substitution. The local wastewater treatment plant WWTP was modeled as a reference. Secondly, a urine recycling system was introduced to treat 75 % of the collected urine, with the remainder piped to the WWTP. Thirdly, a black and greywater (BW&GW) treatment system handling all generated wastewater was examined. Finally, a hybrid source-separating system combining urine, black, and greywater was investigated. The results indicated that the four scenarios exhibited global warming potentials (GWP) of 78, 62, 32, and 24 kg CO2-eq per PE/ y. Recycling urine as fertilizer led to a 20 % reduction in the GWP of the reference. It also reduced other impact categories, with a 55 %, 65 %, and 45 % reduction in eutrophication, ozone depletion, and acidification, respectively. The BW&GW system achieved a 60 % reduction over the reference GWP, mainly due to fertilizer, biogas, and cleanwater recovery. Integrating urine, black, and greywater recycling in the final scenario achieved a 25 % reduction compared to the BW&GW scenario, primarily due to lowering of the ammonia stripping GWP and the additional fertilizer recovery. Based on sensitivity analyses, switching citric acid for sulfuric acid reduced the GWP of the urine stabilization unit process by 101 %, from 15.47 to -0.14 kg CO2-eq per PE/ y. Ultimately, the findings suggest that the fully decentralized source-separating sanitation system incorporating urine, blackwater, and greywater recycling, particularly when combined with 70 % energy recovery at the urine concentrator, is most favorable.

Effect of Nitrogen and Sulphur Fertilization on Winter Oilseed Rape Yield

Oilseed rape is one of many crops with high nutritional requirements, particularly for nitrogen (N) and sulphur (S). Both macronutrients affect important physiological plant functions and are essential for the proper growth and development of oilseed rape. The objective of the experiment was to investigate the impact of nitrogen and sulphur fertilization on the yield of the winter oilseed rape cultivar LG Absolut. The experiment was conducted during the 2019/2020, 2020/2021, and 2022/2023 growing seasons on Haplic Cambisol soil formed from loess, with medium levels of mineral nitrogen and sulphur. In the experiment, two nitrogen fertilization treatments (150 and 200 kg ha−1) were compared in combination with three additional sulphur fertilization rates (20, 40, and 60 kg ha−1). The results demonstrated that the effectiveness of N and S fertilization varied between individual years. On average, the highest seed yields were obtained with the application of 200 kg N ha−1 combined with sulphur, regardless of the rate. This was attributed to a significant increase in soil–plant analysis development (SPAD) values, the number of pods per plant, and the thousand-seed weight. The increase in seed yield with the higher nitrogen rate without sulphur ranged from 0.36 to 0.57 t ha−1 compared to the lower rate (control 150 N kg ha−1). Supplementary sulphur fertilization increased seed yield ranging from 0.22 to 0.76 t ha−1. The protein content in the seeds increased, while the fat content decreased, following the application of the higher nitrogen rate. The decrease in fat content was mitigated by higher rates of sulphur. The application of 60 kg S ha−1 yielded similar results of the tested parameters to the lower rates. Therefore, for soils with moderate levels of mineral nitrogen and sulphur, it is recommended to fertilize winter oilseed rape with 200 kg N ha−1 combined with 20 or 40 kg S ha−1.

Effect of long term conservation agriculture and nitrogen management on soil nitrogen, phosphorus and sulfur fractions under maize–wheat–mungbean cropping system

Conservation agriculture (CA) covers nearly 3.5 mha in Indian. And there is lack of studies on the CA effects soil nutrient dynamics. So, this study examines the effects of nine years of conservation agriculture on soil nitrogen (N), phosphorus (P), and sulfur (S) fractions in an Inceptisol under maize–wheat–mungbean cropping system. Different tillage and nitrogen management practices were evaluated to understand how nutrient management in CA, contrasts with conventional methods. The CA practices and N fertilizer addition significantly enhanced the soil mineral N, alkali-permanganate N, potentially mineralizable N and microbial biomass N. The CA and N fertilization enhanced soluble and loosely bound P by dissolving the iron-bound, aluminum-bound, reductant soluble and calcium-bound P. All the soil S fractions i.e. available S, total water-soluble S, heat soluble S, sodium bicarbonate extractable S, inorganic sulfur, organic sulfur and total sulfur was positively affected by CA and N fertilization practices over conventional tillage and control plots, respectively. It is important to note that the green seeker-based N management options especially with urea super granules showed tendency in enhancing the available nutrient pools of N, P and S compared to recommended dose of N (RDN) applied and control plots indicating that in due course of time it may reduce the amount of RDN without hindering any soil nutrient dynamics. These findings contribute crucial knowledge for sustainable development by offering valuable perspectives on N, P and S management strategies.

Subterranean clover growth responded to grass suppression, phosphorus and molybdenum fertilisers in a summer dry hill environment

Pastures on the north-facing slopes of the Port Hills, Canterbury, are summer-dry, grass dominant, and have low soil phosphorous and sulphur fertility. The aim of this experiment was to improve the establishment of oversown subterranean (sub) clover by the addition of fertilizer and grass-supressing herbicide. Treatments were a factorial combination of phosphorous (0 or 60 kg P/ha, -P or +P), sulphur (0 or 50 kg S/ha, -S or +S), and molybdenum (0 or 0.06 kg Mo/ha, -Mo or +Mo), with or without autumn herbicide (plus or minus, +H or -H). Fertilisers were applied in 2021, with P and S reapplied in 2023, and herbicide applied in 2022 and 2023. Pasture yields were measured annually from 2021 to 2023. Measurements in 2021 found no fertiliser effect on total pasture yield of 3650±83 kg DM/ha that included 770±43 kg of sub clover. In 2022, P addition increased total pasture yield from 2900 to 3270 kg DM/ha. Herbicide application doubled the sub clover yield from 310 to 645 kg DM/ha and reduced grass from 1650 to 1410 kg DM/ha. In 2023, the herbicide application did not affect the total accumulated pasture yield (10180±160 kg DM/ha) but increased sub clover content from 10 to 25% and reduced the grasses from 63 to 47%, with about 4% white clover. Where herbicide was applied without fertiliser (+H), total clover yield increased from 1460 to 2930 kg DM/ha. The application of P and Mo, (i.e., +H+P+Mo treatment) increased it further to 4010 kg DM/ha. There was no yield response to +S or +Mo unless added with P. Applying herbicide or phosphorous increased total metabolisable energy from 38.5 to 42.6 GJ ME/ha and crude protein from 500 to 595 kg/ha in pastures sampled in November 2023. The treatment combination providing the highest feed quality values was +H+P+Mo. These results suggest that to optimise sub clover content of pastures on the Port Hills competing grasses must be controlled initially, followed by P fertiliser if soil tests indicate it is required along with molybdenum.

Unveiling the influences of P fertilization on bioactive compounds and antioxidant activity in grains of four sorghum cultivars.

Phosphorus is a critical nutrient in agriculture, influencing plant growth and nutritional quality. This study, uniquely designed to investigate the effects of phosphorus (P) fertilization levels, sorghum cultivars, and growing locations on phytochemical content and antioxidant activity in sorghum grains, employed four sorghum cultivars (Hakeka, P954063, Tabat, and Tetron) grown under three P levels (0P, 1P, 2P) in two locations (Gezira and White Nile) in Sudan. In this study, four sorghum cultivars were grown in two distinct locations in Sudan, employing a split-plot design with three (P) fertilization levels. P was applied as triple super phosphate directly with the seeds, and additional fertilization included urea applied in two split doses. At physiological maturity, representative sorghum panicles were harvested, processed, and analyzed for bioactive compounds and antioxidant activities using standard extraction and quantification techniques such as Folin-Ciocalteu for phenolics and colorimetric flavonoid assays. Antioxidant activities were assessed through various assays, including DPPH and FRAP. Statistical analyses were performed using a three-way ANOVA to examine the effects of cultivar, P level, and location on the measured parameters, supplemented by multivariate analysis to further elucidate the interactions between these factors. Significant interactions (p<0.001) were observed among cultivars, P levels, and locations for total phenolic content (TPC), total flavonoid content (TFC), carotenoids, tannins, and various antioxidant activity measures (DPPH, FRAP, ABTS, TRP, H2O2). P fertilization significantly increased all measured phytochemicals and antioxidant activities compared to non-treated cultivars, except for H2O2, which decreased with P application. Among cultivars, Hakeka consistently exhibited the highest TFC, carotenoid content, and antioxidant activities (DPPH, FRAP, TRP, ABTS), particularly at the 2P level. P954063 showed the highest TPC and tannin concentrations. Tetron generally had the lowest phytochemical and antioxidant levels. White Nile showed higher TPC, carotenoids, DPPH, FRAP, TRP, and ABTS levels, while Gezira had higher TFC, tannins, and H2O2 concentrations. The impact of phosphorus fertilization often varies between locations. Strong positive correlations were found between TPC and all antioxidant assays (r = 0.68-0.90) and total carotenoids and antioxidant activities (r = 0.73-0.93). This study recommended cultivating the Tabat variety with 2P doses in Gezira. In addition, the Hakeka cultivar showed the highest increases in total flavonoid content, carotenoids, and antioxidant activities, particularly under the highest P level (2P). The findings highlight that P plays a critical role in enhancing sorghum's nutritional and health-promoting qualities, which are essential for leveraging this staple crop for food and nutrition security strategies in semi-arid regions.

Field trial guidelines for evaluating enhanced efficiency fertilizers

AbstractThere are many fertilizer additives and alternatives that aim to increase plant nutrient use efficiency and reduce nutrient losses to the environment, here referred to collectively as enhanced efficiency fertilizers (EEFs). However, there is often insufficient published scientific field trial results across a variety of locations, climates, soils, cropping systems, and management scenarios to prove their efficacy and conditions for use. Guidelines for common minimum datasets and data stewardship in evaluating the agronomic performance and environmental impact of EEFs are needed for researchers to follow. Such guidelines will improve hypothesis testing centered on product efficacy and provide producers with guidance on how these technologies function and perform when integrated with other management practices within the 4R Nutrient Stewardship Framework. A scientific committee was formed to develop a set of protocol guidelines for evaluating EEFs in replicated, plot‐based field trials on an international scale. The guidelines are composed of experimental design and core metadata, crop and soil analyses, environmental loss measurements, and data stewardship, and include both recommended and required components to allow for flexibility and adaptability depending on the trial location, objectives, infrastructure capacity, product type, and depth of understanding of the potential EEF efficacy. This approach will ensure consistency and compatibility in experimental design and data collection to support data integration, analysis, and reuse leading to large‐scale impact and end‐user confidence.

Available Nitrogen Nutrient Levels (NO3-) with Organic Fertilizer Treatment and Its Correlation to the Growth and Yield of Peanut Plants (Arachis hypogaea L.)

Peanut plants require Nitrogen nutrient content to increase plant growth and maximum production. Increasing nitrogen nutrients in the soil can be obtained through the addition of organic fertilizers. The purpose of this study was to determine the effect of organic fertilizers (burnt rice husks and chicken manure) on NO3- nutrient levels in peanut plants, to determine organic fertilizers (burnt rice husks and chicken manure) that will provide the highest NO3- nutrient levels in peanut plants (Arachis hypogaea L.) and to determine the correlation between NO3- nutrient levels with the growth and yield of peanut plants (Arachis hypogaea L.). This study was conducted from September to November 2022 in Toto Utara Village, Tilongkabila District, Bone Bolango Regency. The study used a Randomized Kelompaok Design (RAK) consisting of 3 treatment levels, namely P0 = without organic fertilizer, P1 = chicken manure 3kg/plot, and P2 = burnt rice husk fertilizer 3kg/plot. Each treatment was repeated 4 times so that there were 12 units/plot. The results of this study indicate that organic fertilizers have a significant effect on NO3- nutrient levels with NO3- nutrient levels in the chicken manure treatment of 0.89 (ppm) and in the burnt rice husk fertilizer treatment of 0.61. There is a strong and very strong positive linear correlation between plant height, number of pods, seed weight per plant, and seed weight per hectare with NO3- nutrient levels.

Evaluation of nitrogen use efficiency and N-supplying capacity on pomelo (Citrus grandis L. Osbeck) grown on alluvial soil in the Cuu Long Delta

The study was conducted to determine the nitrogen use efficiency (NUE) and the ability to provide nitrogen from alluvial soil for growing pomelo (Citrus grandis L. Osbeck) in the Mekong Delta. The experiment was arranged in a randomized complete block design including two treatments (NPK treatment: 673 N - 385 P2O5 -192 K2O g/plant and PK treatment: 0 N - 385 P2O5 - 192 K2O g/plant) and three replicates (3 plants for each replicate). The results showed that fruit yield was highest (39.1 kg/plant, N recovery efficiency of 53.8%) in the full NPK fertilization treatment and lowest in the PK fertilization treatment (18.5 kg/plant). Calculation results of the ability to supply nitrogen to pomelo trees in Chau Thanh, Hau Giang showed that alluvial soil had the ability to provide 49.6% of N and the remaining 50.4% from additional fertilizer sources for maximizing fruit yield.