Effects of exogenous nitrogen addition on soil organic nitrogen fractions in different fertility soils: Result from a 15N cross-labeling experiment

Abstract

Exogenous nitrogen (N) addition serves as a pivotal nutrient management strategy, significantly enhancing agricultural production by regulating soil N availability and retention. However, the dynamics of soil organic nitrogen (SON) fractions in response to various forms of exogenous N addition across differing soil fertility levels remain inadequately understood. This study utilized data from a 25-year fertilization experiment and a 15N cross-labeling experiment in Northeast China to assess and quantify the effects of mineral N fertilizers and organic materials (manure and straw) on SON fractions in NPK (mineral fertilizer addition) and NPKM (NPK combined with composted pig manure) treatments. Our findings indicate that long-term incorporation of manure substantially elevates soil fertility compared to the exclusive use of mineral fertilizers. Notably, exogenous N primarily boosts soil N availability by enhancing acid-soluble organic N fractions, particularly ammonium nitrogen (AN) and amino acid nitrogen (AAN). Organic materials, particularly straw, significantly enhanced the retention of mineral fertilizer N in both NPK and NPKM treatments (9.54% vs 10.70%). Moreover, over 70% of the N from straw or manure remained in the soil as stable SON fractions. While straw rapidly improves low-fertility soils, manure contributes to enhanced soil N reserves and increased crop yields. Therefore, incorporating organic matter may bolster soil N sequestration in Northeast China, which is contingent upon soil fertility and tailored fertilizer management strategies. This research elucidates the distribution and conversion of exogenous N within SON pools, facilitating optimized N management, sustaining yields, reducing farmland N pollution, and promoting agricultural sustainability.