Additional Specimens Research Articles

Redescription of Arabella iricolor (Montagu, 1804) with descriptions of two new species from the United Kingdom and South Africa

Arabella iricolor (Montagu, 1804) was described with no diagnostic information for the characters used today to distinguish species within the genus. An updated description, together with genetic data, is provided using the holotype in conjunction with additional specimens collected at or near the type locality, to facilitate comparative analysis of other Arabella species and improve taxonomy within the genus. Arabella iricolor can be distinguished primarily through the long notopodial cirri that reach to or beyond the prechaetal lobes on chaetigers 1–9, in combination with unidentate maxilla I on both sides, four short digitiform pygidial cirri and gradually tapering ventralmost chaetae. The updated description and molecular information enabled the identification of a new species co-habiting with A. iricolor at the type locality and elsewhere. The new species, Arabella ampulliformis sp. nov., is characterised by two lateral pygidial lobes that extend into short cirri, appearing ampulliform in lateral view, in combination with a bifid left maxilla I, short left and long right maxilla II, short notopodial cirri and gradually tapering ventralmost chaetae. A new species from South Africa is also described, Arabella umgazanae sp. nov., distinguished through a bifid left maxilla I, short left and long right maxilla II, short notopodial cirri, two large lateral pygidial lobes and gradually tapering ventralmost chaetae along with additional characters relating to the chaetae, mandibles and maxillary apparatus. Problems surrounding the identification of Arabella species in South Africa are discussed in conjunction with details of the other species reported from the region. ZooBank: urn:lsid:zoobank.org:pub:9E60941E-7F95-4327-8884-5A56A5E7F309

Abstract 3764: Spatially resolved immune landscape revealed sex-biased T-cell infiltration in glioblastoma

Abstract Glioblastoma (GBM) multiforme is the most aggressive type of brain cancer. These lethal brain tumors are characterized by the inter- and intra-tumor molecular heterogeneity and demonstrated cell plasticity. A male:female distribution of 1.6:1; survival outcome from treatment (debulking, radiation, and temozolomide) benefits females nearly twice that for males. Through spatial transcriptomics, we interrogated the immune landscape of GBM, seeking to tease out whether distinct biological and therapeutic niches in GBM were evident, and to understand how the tumor microenvironments contributed to the heterogeneity of GBM. We designed a set of RNA hybridization probes specifically for portraying the cell identities and cell functional states within GBM tumor. The probe set was used to profile six human GBM samples from male and female cohorts on the Vizgen MERSCOPE platform. An integrated analysis of 505,614 cells from the six samples yielded a rich and varied spatial cell atlas of glioblastoma. Our preliminary findings depict distinct spatial niches of different tumor cell subpopulations that are associated with local environmental factors such as hypoxia. We identified a small cluster of cells that are in neuronal-like state, which could potentially drive invasion. We found spatial proximity of CD4+ cells and cells that express HIF1A, suggesting the dynamic interactions between tumor cells, regulatory T cells and tumor microenvironment. We also observed significant difference in the degree of CD8+ T-cell infiltration in female samples versus male samples. The average CD4+/CD8+ T-cell ratio in males is twice as high as that of females, suggesting intrinsic sex-based differences in immune response to tumor. Expansion of these studies in additional specimens will further illuminate microenvironmental influences on glioma progression and may instruct specific interventions leading to more effective and predictable immune-oncology of this disease. Citation Format: Yue Hao, George Reid, Angela Baker, Karen L. Fink, George J. Snipes, Bruce E. Mickey, Andrew E. Sloan, Jill S. Barnholtz-Sloan, Michael Berens. Spatially resolved immune landscape revealed sex-biased T-cell infiltration in glioblastoma [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 1 (Regular Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(6_Suppl):Abstract nr 3764.

Aedes unalom sp. nov. in Cambodia, a new Stegomyia species close to Aedes albopictus (Diptera, Culicidae)

Preliminary genetic analysis of cytochrome c oxidase subunit 1 (COI) gene of specimens initially assigned to the mosquito Aedes albopictus based on morphology revealed two clusters. This observation led to further genetic and morphological analysis: a total of 86 Stegomyia specimens identified morphologically as Ae. (Stegomyia) albopictus, were collected between 2017 and 2019 in Cambodia. Their mitochondrial DNA COI gene and 5.8S-ITS2 rDNA were sequenced for molecular characterization and genetic analysis as well as Wolbachia presence/type. In 2022, additional Stegomyia specimens were collected for COI sequencing and morphological characterization. Of the 86 Stegomyia specimens, 14 specimens had divergent COI and 5.8S-ITS2 sequences compared to other Stegomyia species, and were negative for the presence of Wolbachia, unlike Ae. albopictus specimens which carried wAlbA and/or wAlbA B types. Additional trapping in 2022 resulted in the colletion of 11 specimens with similar divergent COI and 5.8S-ITS2 sequences. Sequence blast results did not match these COI and 5.8S-ITS2 sequences, neither within Stegomyia taxa nor other organisms. Lastly, after morphological examinations, specific characters were observed in adult mosquitoes and male genitalia, allowing the specimens to be described as a new Stegomyia species named Aedes unalom Maquart & Hide, sp. nov. This species lives in sympatry with other Stegomyia species such as Ae. albopictus or Ae. aegypti and does not carry Wolbachia. This new species should be considered as a potential arbovirus vector.

Optimization of critical process control parameters in MEX additive manufacturing of high-performance polyethylenimine: energy expenditure, mechanical expectations, and productivity aspects

The demand for 3D-printed high-performance polymers (HPPs) is on the rise across sectors such as the defense, aerospace, and automotive industries. Polyethyleneimine (PEI) exhibits exceptional mechanical performance, thermal stability, and wear resistance. Herein, six generic and device-independent control parameters, that is, the infill percentage, deposition angle, layer height, travel speed, nozzle temperature, and bed temperature, were quantitatively evaluated for their impact on multiple response metrics related to energy consumption and mechanical strength. The balance between energy consumption and mechanical strength was investigated for the first time, contributing to the sustainability of the PEI material in 3D printing. This is critical considering that HPPs require high temperatures to be built using the 3D printing method. PEI filaments were fabricated and utilized in material extrusion 3D printing of 125 specimens for 25 different experimental runs (five replicates per run). The divergent impacts of the control parameters on the response metrics throughout the experimental course have been reported. The real weight of the samples varies from 1.06 to 1.82 g (71%), the real printing time from 214 to 2841 s (~ 1300%), the ultimate tensile strength from 15.17 up to 80.73 MPa (530%), and the consumed energy from 0.094 to 1.44 MJ (1500%). The regression and reduced quadratic equations were validated through confirmation runs (10 additional specimens). These outcomes have excessive engineering and industrial merit in determining the optimum control parameters, ensuring the sustainability of the process, and the desired functionality of the products.Graphical

Изучение эволюции микроструктуры и механических свойств в алюминиевом сплаве 1570 с добавкой 0,5 % гафния

Introduction. Aluminum alloys are in high demand with the aerospace industry. From the viewpoint of various performance characteristic combinations, high-magnesium aluminum alloys with the addition of transition metals, such as Zr and Sc, are among the most future-oriented alloys. Alloy 1570 is one of the most popular in this group. Recent studies demonstrated the positive effect of 0.5 % hafnium addition on as-cast structure. Study objective is to study the effect of the addition of 0.5% hafnium on the structure and properties of aluminum alloy 1570 during thermomechanical treatment. The study addresses the effect of cold rolling, homogenization, and recrystallization annealing on mechanical properties and microstructure of the specimens from alloy 1570 and similar alloy with 0.5 wt. % hafnium addition. Study methodology: for the study, ingots were cast from alloy 1570 with and without additions of 0.5 wt. % of hafnium. The resulting ingots were homogenized for 4 h at 440 °С, followed first by hot rolling and then cold rolling. Cold-rolled specimens were annealed at temperatures 340 °С to 530 °С with a holding time of 3 hours. The homogenized, cold-rolled, and annealed specimens were examined using transmission and light microscopy. In addition, homogenized and cold-rolled specimens were subjected to uniaxial tensile tests to determine the mechanical properties of the studied alloy. Results and discussion. It is revealed that in an alloy containing hafnium, after homogenization annealing, there is a slight decrease in the average particle size and an increase in its total proportion in comparison with alloy 1570. In general, 0.5 % hafnium addition does not significantly affect the mechanical properties. The number of nanoparticles in both alloys increases, as does the yield strength compared to the as-cast state. When heated, both alloys demonstrate an increase in plasticity and a decrease in strength characteristics. Studies of the annealing effect on the grain structure of the studied alloys showed that hafnium increases the tendency of alloy 1570 to recrystallize. However, additional research is required to determine the reasons for this phenomenon.

Rapid fatigue evaluation of additive manufactured specimens: Application to stainless steel AISI 316L obtained by laser metal powder bed fusion

Laser powder bed fusion additive manufacturing is a promising technology for manufacturing components in many industrial applications. These components are often subjected to cycling loading and there are concerns about their fatigue performance due to inherent anisotropy, defects, and surface roughness. However, conventional fatigue assessment is time-consuming and expensive. Consequently, several attempts have been made in the last decades to reduce the time and cost associated with these tests by introducing the concept of rapid fatigue testing. Most of these accelerated methods necessitate advanced laboratory equipment and high-precision tests. Recently, a novel approach to rapid fatigue assessment, the stiffness method, has emerged, based on evaluating damage accumulation during the test. This method has been successfully applied and validated across a wide range of wrought metal sheets. In this study, the stiffness method is deployed to evaluate the fatigue performance of additively manufactured stainless steel AISI316L. The results are compared with predictions of the empirical model based on Murakami's approach and the conventional staircase method. A good agreement is obtained between both approaches, showing the stiffness method can estimate the fatigue limit of additive manufacturing specimens in a reliable, fast, and cost-effective way while using a significantly reduced number of tests.

The tanan [Chlorocoelus hypericifolius (Stoll 1813): Orthoptera, Tettigonioidea, Pseudophyllinae]re-discovered more than 150 years after the description of the male by Bates and the figuring by Darwin.

In his famous book, The descent of man and selection in relation to sex, Darwin (1871) described the male secondary sexual characters of Orthoptera in some detail. As the most prominent example, he presented a figure of a South American bush-cricket species. It had been described a few years before by Bates and was called, onomatopoetically, Chlorocoelus tanana. Surprisingly, the fascinating species has never been found since this time, although in old collections some additional specimens were located. Based on recently collected material and information on the internet, we clarify the taxonomy of the species and give details of the male stridulatory organ and its distribution. The peak frequency (2.1 kHz) of its resonant disturbance sounds indicates that also calling song may have an unusually low carrier frequency similar to other large bush-crickets with extraordinarily inflated tegmina.

Morphology and Molecular Phylogeny of the Genus Stigeoclonium (Chaetophorales, Chlorophyta) from China, Including Descriptions of the Pseudostigeoclonium gen. nov.

Stigeoclonium is a genus of green algae that is widely distributed in freshwater habitats around the world. The genus comprises species with variously developed prostrates and erect systems of uniseriate branched filaments and grows attached to a wide range of different surfaces. It holds significant promise for applications in water quality indicators, sewage treatment, and the development of high-value-added products. Nevertheless, our comprehension of Stigeoclonium remains unclear and perplexing, particularly regarding its fundamental systematic taxonomy. Recent molecular analyses have revealed that the morphologically well-defined genus Stigeoclonium is polyphyletic and requires taxonomic revision. Phylogenetic analysis based on a single molecular marker and limited samples is insufficient to address the polyphyletic nature of Stigeoclonium. In the present study, 34 out of 45 strains of Stigeoclonium were newly acquired from China. Alongside the morphological data, a concatenated dataset of three markers (18S rDNA + ITS2 + tufA) was utilized to determine their molecular phylogeny. The phylogenetic analysis successfully resolved the broadly defined Stigeoclonium into three robustly supported clades (Stigeoclonim tenue clade, S. farctum clade, and S. helveticum clade). The morphological characteristics assessment results showed that the cell type of the main axis-producing branch, considered a crucial morphological characteristic of the Stigeoclonium taxonomy, did not accurately reflect the real phylogeny of the genus. A new taxonomical classification of the genus Stigeoclonium was proposed based on zoospores' germination types, which aligned well with the phylogenetic topologies. Species where zoospores showed erect germination (S. helveticum clade) formed a distinct monophyletic clade, clearly separated from the other two clades, with zoospores showing prostrate germination or pseudo-erect germination. Consequently, a new genus, Pseudostigeoclonium gen. nov., is suggested to include all species in the broadly defined Stigeoclonium with zoospores with erect germination. The taxonomic diversity is supported by distinctive morphological differences and phylogenetic divergence within the broadly defined Stigeoclonium identified in this study. Further evaluation of the genus Stigeoclonium is necessary, especially via examining additional specimens and re-evaluating morphological characters under precisely defined laboratory conditions.

Effect of Layer Addition on Residual Stresses of Wire Arc Additive Manufactured Stainless Steel Specimens

Abstract Residual stresses have been characterized in four Wire Arc Additive Manufacturing specimens with neutron diffraction technique. First, two methods are investigated for obtaining the reference diffracted angle θ0 that is required for the computation of microstrains and, thus, the stresses; θ0 was obtained with two approaches. The first one required a strain-free specimen in order to get directly the reference diffracted angles θ0 in the three principal directions. The second one is based on the plane stress assumption to get θ0 indirectly by imposing that the normal stress was equal to zero. Both methods led to similar residual stress profiles for the one-layer specimen which validated this approach for all specimens without a strain-free specimen available. The second part of this work focused on the modification of the residual stresses in the specimen following the addition of a new deposit. The neutron diffraction measurements showed that the longitudinal stress was tensile in the heat-affected and fusion zones with a maximum value located at the parent material–layers interface where the thermal loadings were applied. A decrease of this maximum value from 257 MPa to 199 MPa appeared after deposition of a new layer which is due to some stress relaxation effect. Inside the parent material, a large zone presents compressive longitudinal stress up to −170 MPa. The bottom part of the parent material is under tensile stress likely due to its upward bending following the thermal contraction of the deposited layers during cooling to ambient temperature.

New records of immature aquatic Diptera from the Foulden Maar Fossil-Lagerstätte, New Zealand, and their biogeographic implications.

The biogeographical and ecological history of true flies (Diptera) in New Zealand is little known due to a scarcity of fossil specimens. Here, we report a fauna of immature aquatic dipterans from freshwater diatomites of the early Miocene Foulden Maar Fossil-Lagerstätte in southern New Zealand. We document 30 specimens of immature dipterans, mostly pupae, and compare their external morphology to extant aquatic Diptera. Based on the reconstructed paleoenvironment of Foulden Maar, we discuss taxonomic, ecological and taphonomic implications of this early Miocene fauna. Among Chironomidae, one pupal morphotype is attributed to Tanypodinae, one pupal morphotype and one larval morphotype are placed into Chironomus (Chironominae) and a further morphotype into Chironominae incertae sedis. Chaoboridae are represented by a pupal morphotype congeneric or very close to the extant Chaoborus, today globally distributed except for New Zealand. Additional immature specimens are likely larvae and puparia of brachyceran flies but cannot be identified to a narrower range. These finds document an aquatic dipteran fauna in New Zealand in the earliest Miocene and highlight Neogene extinction as a factor in shaping the extant Diptera fauna in New Zealand. Immature aquatic dipterans were a common and likely ecologically important component of the early Miocene Foulden Maar lake. Preservation of larvae and pupae may have been promoted by diatomaceous microbial mats and the light colour of the diatomite likely facilitated spotting of these minute fossils in the field.

Upper Cretaceous Crustacea from Central Cuba

We report new occurrences and additions to previously described species of decapod crustaceans from the Late Cretaceous (Campanian-Maastrichtian) of Cuba. Three of the eight species herein described and illustrated were published previously: Vegaranina rivasi Arano-Ruiz et al.2018; Graptocarcinus collinsi Vega et al., 2020; and Icriobranchiocarcinus rodas Vega et al., 2021; however additional specimens are included. New records include one indeterminate lobster, two new genera and three new species of raninoids, and the first report of Ophthalmoplax cf. O. brasiliana Maury 1930 from Cuba. The most numerous specimens correspond to raninoids, which although not complete, can be described based on complementary pieces of the dorsal carapace and front, and they are assigned as follows: Aguadites hexagranula Vega, Garassino, Nyborg, and Pasini n. gen. sp.; Rodanina monosensis Vega, Garassino, Nyborg, and Pasini n. gen et sp., and Raninoides granulofrons Vega, Garassino, Nyborg, and Pasini n. sp., which represents the oldest record for the genus. Vegaranina rivasi is compared to V. precocia (Feldmann, Vega, Tucker, García-Barrera and Avendaño, 1996) from the Maastrichtian of Chiapas, Mexico, confirming different characters to keep both species as representatives of Vegaranina Van Bakel, Guinot, Artal, Fraaije and Jagt 2012a. The astacid lobster is represented by one isolated carapace (part and counterpart), preserved in a concretion. Two complete dorsal carapaces (one preserving the right cheliped) of Graptocarcinus collinsi represent additional information on the morphology of this species. An incomplete specimen of Ophtalmoplax cf. O. brasiliana, confirms the extensive distribution of this species during Maastrichtian times in the Atlantic shallow marine environments, from Brazil to North Carolina, USA, representing the missing link of the distribution to be found in the Caribbean province. A dorsal carapace is assigned to Icriobranchiocarcinus rodas , since it shows the typical transverse ridges of the species. This report represents an important addition to the knowledge of the Cretaceous crustaceans from Cuba and the Caribbean, confirming that this paleobiogeographic province was composed by a quite different assemblage from those reported from the same age (Campanian-Maastrichtian) of nearby regions such as NE Mexico, SE USA and NE South America.

The genus Perissopalla Brennan and White, 1960 (Trombidiformes: Trombiculidae) in Brazil: Redescriptions, new localities, and host records with the first molecular data for the genus

The genus Perissopalla Brennan and White (Trombidiformes: Trombiculidae) is currently represented by ten species with three from Brazil: Perissopalla barticonycteris Brennan, Perissopalla ipeani Brennan, and Perissopalla tanycera Brennan. In the present study, these three species are redescribed and illustrated based on the types and additional non-type specimens. New host and locality records for P. ipeani and P. tanycera are included. Additionally, a partial sequence for the 18S rRNA gene for P. ipeani was provided.

Taxonomic study on Afrotropical Osoriinae (Coleoptera: Staphylinidae). Status of Mimogonellus Fagel and Madegassosorius Scheerpeltz.

Among Afrotropical genera of Osoriini, Madegassosorius Scheerpeltz is exceptionally poorly defined. Although later authors maintained this genus, they restricted its diagnostic features and distinguish it from the apparently highly similar Mimogonellus Fagel merely by the structure or presence of the lateral pronotal carinae and the elytral adsutural striae. A comprehensive survey of specimens belonging to most world genera of Osoriini suggested that these characters may be variable within the genus and therefore of weak taxonomic value. In order to test this hypothesis, the type species of Madegassosorius and Mimogonellus were studied in detail, as well as dozens of additional specimens belonging to this unclearly defined group. As a result, Madegassosorius syn. n. is placed as a junior synonym of Mimogonellus, and the latter genus is redefined and redescribed. A lectotype is designated for Mimogonus leleupi Cameron, the type species of Mimogonellus. The synonymization of Madegassosorius with Mimogonellus results in one case of secondary homonymy, and Mimogonellus longipennides nom. n. is proposed as a replacement name for Mimogonellus longipennis (Coiffait, 1979) comb. n. (ex Madegassosorius), nec Mimogonellus longipennis Fagel, 1960.

Updated checklist of treehoppers of Panama (Hemiptera: Membracidae) with plant associations.

An updated checklist of treehoppers (Hemiptera: Membracidae) of Panama is provided with known province distribution data. The world catalogue of Membracoidea, through April, 1997, lists 68 genera, 162 species, and 1 subspecies from Panama (McKamey, 1998). Flynn (2012) listed all known species from Panama, documenting range extensions for 29 genera, 107 species, and 1 subspecies previously unknown from Panama, bringing the treehopper fauna from Panama to 97 genera, 269 species, and 2 subspecies. Inspection of 768 additional specimens since 2012 (new total of 3,595 specimens for the study), in addition to published literature, has added 2 new genera and 40 new species bringing the treehopper fauna to 100 genera (increase of 3 genera), 313 species (increase of 44 species) from 2012, and 2 subspecies. Many new provincial sites were added to each species with an updated provincial map showing numbers of species in each province. Updated species counts per Province (with increase): Bocas del Toro 65 (+10), Chiriqui 162 (+11), Veraguas 26 (+3), San Blas 8 (+1), Herrera 9 (+1), Darien 72 (+13), Los Santos 4 (+1), Cocle 43 (+9), Colon 187 (+79), and Panama 150 (+32). Tables listing published checklists and keys for Nearctic and Neotropical treehoppers are presented.

Metagenomic next-generation sequencing of plasma cell-free DNA improves the early diagnosis of suspected infections

BackgroundMetagenomic next-generation sequencing (mNGS) could improve the diagnosed efficiency of pathogens in bloodstream infections or sepsis. Little is known about the clinical impact of mNGS test when used for the early diagnosis of suspected infections. Herein, our main objective was to assess the clinical efficacy of utilizing blood samples to perform mNGS for early diagnosis of suspected infections, as well as to evaluate its potential in guiding antimicrobial therapy decisions.MethodsIn this study, 212 adult hospitalized patients who underwent blood mNGS test in the early stage of suspected infections were enrolled. Diagnostic efficacy of mNGS test and blood culture was compared, and the clinical impact of mNGS on clinical care was analyzed.ResultsIn our study, the total detection rate of blood mNGS was significantly higher than that of culture method (74.4% vs. 12.1%, P < 0.001) in the paired mNGS test and blood culture. Blood stream infection (107, 67.3%) comprised the largest component of all the diseases in our patients, and the detection rate of single blood sample subgroup was similar with that of multiple type of samples subgroup. Among the 187 patients complained with fever, there was no difference in the diagnostic efficacy of mNGS when blood specimens or additional other specimens were used in cases presenting only with fever. While, when patients had other symptoms except fever, the performance of mNGS was superior in cases with specimens of suspected infected sites and blood collected at the same time. Guided by mNGS results, therapeutic regimens for 70.3% cases (149/212) were changed, and the average hospitalized days were significantly shortened in cases with the earlier sampling time of admission.ConclusionIn this study, we emphasized the importance of blood mNGS in early infectious patients with mild and non-specific symptoms. Blood mNGS can be used as a supplement to conventional laboratory examination, and should be performed as soon as possible to guide clinicians to perform appropriate anti-infection treatment timely and effectively. Additionally, combining the contemporaneous samples from suspected infection sites could improve disease diagnosis and prognoses. Further research needs to be better validated in large-scale clinical trials to optimize diagnostic protocol, and the cost-utility analysis should be performed.

Maximizing visible Raman resolution of nanodiamond grains fabricated by coaxial arc plasma deposition through oxygen plasma etching optimization

Among the nondestructive carbon material characterization tools, the prominence of visible Raman spectroscopy has surged remarkably for many years due to its ability to explore a diverse array of carbon bonding configurations. However, to fully unlock the distinctive features concealed within carbon composite materials, additional specimen treatments or precise spectroscope calibrations are necessary. In the same regard, the tiny diamond grain size (5–10 nm) and the pronounced amount of sp2 carbon in the ultrananocrystalline diamond film represent major challenges in visible light excitation. In this work, we employ calibrated oxygen plasma reactive ion etching conditions to manifest the nanodiamond visible Raman signature from ultrananocrystalline diamond/amorphous carbon composite (UNCD/a‐C) films fabricated by coaxial arc plasma deposition. Upon plasma etching, the broad defect band in the visible Raman spectra converged toward the diamond characteristic Raman peak at 1332 cm−1. A detailed explanation of band components of the Raman spectra is extracted through peak fitting procedures. The results of Raman spectroscopy are further correlated with the electrical characteristics of the nitrogen‐doped UNCD/a‐C films due to the optimized oxygen plasma etching processes.

Cranial osteology and paleoneurology of Tarjadia ruthae: An erpetosuchid pseudosuchian from the Triassic Chañares Formation (late Ladinian-?early Carnian) of Argentina.

Tarjadia ruthae is a quadrupedal terrestrial pseudosuchian from the Middle-early Upper Triassic of the Chañares Formation, La Rioja Province, Argentina. Originally, this species was identified as an indeterminate archosaur and later as a doswelliid archosauriform based on very fragmentary specimens characterized by the ornamentation of the skull roof and osteoderms. Additional specimens (including skulls and postcrania) recovered in the last decade show that Tarjadia is an erpetosuchid, an enigmatic pseudosuchian group composed of six species registered in Middle-Upper Triassic continental units of Tanzania, Germany, Scotland, North America, Brazil, and Argentina. Tarjadia ruthae from Argentina and Parringtonia gracilis from Tanzania are the best preserved and more abundant species. Although the monophyly of Erpetosuchidae is well supported, alternative high-level positions within Archosauria have been suggested, such as sister taxon to Crocodylomorpha, Aetosauria, or Ornithosuchidae. In order to improve the knowledge about the erpetosuchids, we performed a detailed description and paleoneurological reconstruction of the skull of Tarjadia ruthae, based on two articulated partial skulls (CRILAR-Pv 478 and CRILAR-Pv 495) and other fragmentary specimens. We analyzed the stratigraphic and geographic occurrence of historical and new specimens of Tarjadia and provided a new emended diagnosis (the same for the genus as for the species, due to monotypy) along with a comparative description of the cranial endocast. The skull of Tarjadia is robust, with a thick and strongly ornamented skull roof, triangular in dorsal view, with concave lateral margins at mid-length that form an abrupt widened posterior region. The external nares are the smallest openings of the skull. The antorbital fossa is deeply excavated and has a small heart-shaped fenestra with both lobes pointing anteriorly. The supratemporal fenestrae are as large and rounded as the orbits, and the infratemporal fenestrae are L-shaped with an extensive excavation along the jugal, quadratojugal and quadrate. The hemimandibles are low, slightly concave on the dentigerous region and strongly convex on the posterior region, conferring them a S-shaped profile in dorsal view. The external mandibular fenestra is small and elliptic, being twice longer than high. The maxillary dentition is restricted to the anterior to mid region of the rostrum. Since the braincase of both specimens is partially damaged, the dorsal surface of the brain could not be entirely reconstructed. As a result, the endocast is anteroposteriorly elongated and seemingly flat, and the cephalic flexure seems to be lower than expected for a suchian. The labyrinth is twice wider than high, the semicircular canals are remarkably straight, and the anterior canal is longer than the posterior one.

Comparison of the specimen quality of endobronchial ultrasound-guided intranodal forceps biopsy using standard-sized forceps versus mini forceps for lung cancer: A prospective study.

Endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA) is a diagnostic procedure with adequate performance; however, its ability to provide specimens of sufficient quality and quantity for treatment decision-making in advanced-stage lung cancer may be limited, primarily due to blood contamination. The use of a 0.96-mm miniforceps biopsy (MFB) permits true histological sampling, but the resulting small specimens are unsuitable for the intended applications. Therefore, we introduced a 1.9-mm standard-sized forceps biopsy (SFB) and compared its utility to that of MFB. We prospectively enrolled patients from three institutions who presented with hilar/mediastinal lymphadenopathy and suspected advanced-stage lung cancer, or those who were already diagnosed but required additional tissue specimens for biomarker analysis. Each patient underwent MFB followed by SFB three or four times through the tract created by TBNA using a 22-gauge needle on the same lymph node (LN). Two pathologists assessed the quality and size of each specimen using a virtual slide system, and diagnostic performance was compared between the MFB and SFB groups. Among the 60 enrolled patients, 70.0% were diagnosed with adenocarcinoma. The most frequently targeted sites were the lower paratracheal LNs, followed by the interlobar LNs. The diagnostic yields of TBNA, MFB and SFB were 91.7%, 93.3% and 96.7%, respectively. The sampling rate of high-quality specimens was significantly higher in the SFB group. Moreover, the mean specimen size for SFB was three times larger than for MFB. SFB is useful for obtaining sufficient qualitative and quantitative specimens.

Investigating the Influence of Holes as Crack Arrestors in Simulating Crack Growth Behavior Using Finite Element Method

The primary focus of this paper is to investigate the application of ANSYS Workbench 19.2 software’s advanced feature, known as Separating Morphing and Adaptive Remeshing Technology (SMART), in simulating the growth of cracks within structures that incorporate holes. Holes are strategically utilized as crack arrestors in engineering structures to prevent catastrophic failures. This technique redistributes stress concentrations and alters crack propagation paths, enhancing structural integrity and preventing crack propagation. This paper explores the concept of using holes as crack arrestors, highlighting their significance in increasing structural resilience and mitigating the risks associated with crack propagation. The crack growth path is estimated by applying the maximum circumferential stress criterion, while the calculation of the associated stress intensity factors is performed by applying the interaction integral technique. To analyze the impact of holes on the crack growth path and evaluate their effectiveness as crack arrestors, additional specimens with identical external dimensions but without any internal holes were tested. This comparison was conducted to provide a basis for assessing the role of holes in altering crack propagation behavior and their potential as effective crack arrestors. The results of this study demonstrated that the presence of a hole had a significant influence on the crack growth behavior. The crack was observed to be attracted towards the hole, leading to a deviation in its trajectory either towards the hole or deflecting around it. Conversely, in the absence of a hole, the crack propagated without any alteration in its path. To validate these findings, the computed crack growth paths and associated stress intensity factors were compared with experimental and numerical data available in the open literature. The remarkable consistency between the computational study results for crack growth path, stress intensity factors, and von Mises stress distribution, and the corresponding experimental and numerical data, is a testament to the accuracy and reliability of the computational simulations.

SWOG S2107: Randomized phase II trial of encorafenib and cetuximab with or without nivolumab for patients with previously treated, microsatellite stable, BRAFV600E metastatic and/or unresectable colorectal cancer.

TPS234 Background: Patients with microsatellite stable (MSS), BRAFV600E metastatic colorectal cancer (mCRC) experience poor survival outcomes. Treatment with the BRAF inhibitor encorafenib (E) and anti-EGFR antibody cetuximab (C) is an approved treatment combination, with a reported overall response rate (ORR) of 20% and median progression-free survival (PFS) of 4.1 months. Anti-PD-1 antibodies like nivolumab (N) are ineffective as monotherapy for patients with MSS, BRAFV600E mCRC. In preclinical models of MSS, BRAFV600E CRC, inhibition of BRAF and EGFR induces a loss of expression of mismatch repairs genes and promotes a microsatellite instability-high phenotype, which may prime these tumors for response to immunotherapy. In support of these findings, a single-institution clinical trial of E + C + N for 26 participants with MSS, BRAFV600E mCRC reported an ORR of 50% and median PFS of 7.2 months. We hypothesize that the addition of N to E + C will improve PFS for participants with MSS, BRAFV600E mCRC. Methods: In this prospective phase II clinical trial (N=75), participants with previously treated MSS, BRAFV600E mCRC will be randomized 2:1 into 2 arms, respectively: experimental treatment (E + C + N) or standard treatment (E + C). No prior BRAF, EGFR, or immunotherapy agents are allowed. All participants will receive E (300 mg PO daily) and C (500 mg/m2 IV every 14 days), and participants in the experimental arm will receive N (480 mg IV every 28 days). The primary endpoint is PFS. Secondary endpoints include overall survival, best overall response, duration of response, and safety/tolerability. Using a one-sided α= .10 and power 80%, we target an improvement in median PFS from 4.2 to 7.3 months and a hazard ratio of 0.57. Additional participants specimens will be collected for exploratory correlative research. As of 9/2023, 37 of 75 planned participants have been enrolled. Clinical trial information: NCT05308446 .