Addition Products Research Articles

A bibliometric analysis using a newly developed model and a customizable research tool: A case study of researcher mobility in Sweden.

Researcher mobility is an integral part of the way research is conducted and of a researcher's career. Its effects on collaboration networks, research impact and knowledge flows drive countries and institutions to quantify and understand this activity. The purpose of this study is to test a new researcher mobility model which was developed and prototyped as a customisable research tool to provide a unified perspective on mobility at macro (national), meso (institutional) and micro (individual) levels. The approach includes multidimensional perspectives, including temporal, geographical, sectoral, directional mobility, that could be used for benchmarking and trend analyses. The model quantifies research mobility volumes and qualifies the mobility flow additional researcher characteristics and productivity indicators. We tested the tool among Sweden's higher education sector, observing researcher mobility patterns between 1992-2021. Results show a high degree of variability in researcher mobility patterns across institutions, especially when considered by career age. Larger higher education institutions in Sweden tend to see a high level of inter-university mobility: most of the Outflow researchers have international mobility and were affiliated with organisations from diverse sectors. Smaller universities are more adapted to attract early- and retain late-career researchers. One university was identified as an incubator for early-career researchers that go on to high levels of mobility. Another university achieved higher mobility rates by facilitating short-term mobility abroad. The study highlighted a shift in the countries of destination for the Inflow early-career researchers: fewer were affiliated with USA, UK or Japan, while other countries became more prominent (China, Germany, Netherlands, Spain) and new destinations emerged (Brazil, India, Iran). The study emphasized that visiting researchers are consistently more productive, and their research impact is generally higher. With the help of our advanced model, we present a detailed picture of mobility in Sweden and demonstrate the power of this customisable tool.

Integrated inactivation of Microcystis aeruginosa and degradation of microcystin-LR by direct current glow discharge plasma in liquid-phase: Mechanisms and cell deactivation process

The frequent occurrence of blooms of Microcystis aeruginosa (M. aeruginosa) and the subsequent release of microcystin-LR (MC-LR) in eutrophic waters pose a serious threat to aquatic ecosystems. This study investigated the optimal conditions for inactivating M. aeruginosa and the degrading MC-LR using direct current glow discharge plasma in liquid phase (DC-LGDP), analyzed the potential inactivation mechanisms and the cell deactivation process of M. aeruginosa. The results showed that DC-LGDP generated reactive species (i.e., •OH, 1O2, and H2O2), active Cl and electroporation effect collectively contributed to inactivation of M. aeruginosa and degradation of MC-LR. The 97.07% inactivation efficiency of M. aeruginosa and 94.98% degradation rate of MC-LR were achieved with higher energy yield and without generating nitrogen oxides. Meanwhile, DC-LGDP destroyed the cell integrity, eliminated their antioxidant capacity and reduced the content of photosynthetic pigments. The transcriptome analysis indicated that the transcripts of genes related to photosynthesis, ribosome biosynthesis, ABC transporters, and nitrogen metabolism pathway in M. aeruginosa were altered by DC-LGDP. This study provides insights into the inactivation of M. aeruginosa by DC-LGDP, while elucidating the potential inactivation mechanisms and the cell deactivation process involved. It may be important for the eco-friendly inactivation of M. aeruginosa blooms in natural water bodies. Environmental implicationsThe frequent occurrence of blooms of M. aeruginosa and the subsequent release of MC-LR in eutrophic waters are a global issue. Although glow discharge plasma technologies have been applied for the inactivation of M. aeruginosa, limitations such as low energy yield and additional nitrous oxide production remain. This study aims to explore the optimal conditions for inactivating M. aeruginosa and degrading MC-LR using direct current glow discharge plasma in liquid phase (DC-LGDP), as well as to analyze the inactivation mechanisms and the cell deactivation process of M. aeruginosa. Our results show that the 97.07% inactivation efficiency of M. aeruginosa by DC-LGDP is achieved at higher energy yield and without the generation of nitrogen oxides. The inactivation of M. aeruginosa and degradation of MC-LR are involved in the reactive species generated by DC-LGDP and electroporation effect. These factors induce oxidative stress damaging the cell integrity, impairing the photosynthesis and ribosome biosynthesis pathways of M. aeruginosa, ultimately leading to the cell deactivation of M. aeruginosa. Our findings provide insights into the effective inactivation of M. aeruginosa and the degradation of MC-LR by DC-LGDP with lower energy consumption and environmental friendliness. Thus, it may be important for the inactivation of M. aeruginosa blooms in natural water bodies.

Synthesis and Cancer Cell Cytotoxicity of 6‐, 7‐, or 8‐Substituted 2‐(Hetero)aryl‐4‐(4‐(Hetero)aryl‐2‐Oxobut‐3‐en‐1‐Ylidene)Benzothiazepanes

ABSTRACTCancer chemotherapy is continuously challenged by serious complications like pronounced side effects and multidrug resistance (MDR). Natural products, such as curcumin, offer promising alternatives due to their diverse biological applications and low toxicity. However, curcumin's clinical utility is limited by poor bioavailability, rapid metabolism, and non‐specific (PAINS) activity. Building on previous findings, this study explored the structural modification of curcumin‐inspired benzothiazepane derivatives in an attempt to enhance their therapeutic potential through modifications of the two peripheral (hetero)aromatic rings and the benzothiazepane scaffold. In this way, eight new 2‐(hetero)aryl‐4‐(4‐(hetero)aryl‐2‐oxobut‐3‐en‐1‐ylidene)benzothiazepanes and two 4‐thiobutan‐2‐one “double Michael addition” derivatives were synthesized and tested for cytotoxicity against a panel of eight cancer cell lines. The screening results indicated that bis‐(4‐hydroxyphenyl) analogs bearing a chlorinated benzothiazepane ring exhibited the highest potency and broad‐spectrum activity at the low micromolar range. Bis‐substitutions with 3‐pyridinyl and 2‐furyl groups showed less potent but more specific activity profiles, potentially reducing PAINS effects. 2‐Aminothiophenol‐derived double Michael addition products demonstrated increased broad‐spectrum activity, highlighting the importance of the free aniline amino group for targeted effects. This study underscores the potential of benzothiazepane derivatives as viable cancer cell cytotoxic agents and provides useful insights for future optimization and evaluation.

Factors influencing the expressed willingness to transition from collection to cultivation of non-timber forest products: The case of Caesalpinia spinosa in southern Ecuador

Planting trees with additional non-timber products (NTFPs) (such as fruits) in agricultural lands is emerging as an important strategy in Forest Landscape Restoration (FLR) to enhance the provision of ecosystem services and improve habitat quality. In many cases, the planting of NTFP trees follows a transition from collecting NTFPs in natural forests to integrating the respective trees into agricultural farms with ecological and socio-economic implications. Therefore, understanding the current practice and challenges of current NTFP collection and willingness for cultivation is crucial to effectively use such land-use trajectories for FLR. In this study, we investigate the case of Caesalpinia spinosa (tara), whose fruits provide an emerging NTFP in southern Ecuador. Using household data from 125 households currently collecting tara fruits, we first describe the relatively young production system and then use a Heckman two-step econometric model to identify the factors influencing the expressed willingness to cultivate tara trees on farms and the area to be cultivated. The econometric model results show that the characteristics of tara collection and trade (gender of collectors, distance to the collection site, and management practices) are important determinants of tara cultivation. However, it's the household labor availability and farm characteristics that play a significant role in impacting the willingness to cultivate. The area to be cultivated was mainly influenced by household labor and economic characteristics, and tara collection and trade (management practices) characteristics. Our findings help to understand NTFP-related land-use transitions, thus supporting the design of appropriate and specific policy interventions for the sustainable development of NTFPs.

Introduction of nicotine analogue-containing oral pouch products in the United States.

In 2023, 6-methyl nicotine (6MN), a synthetic nicotine analog, was introduced in US-marketed electronic cigarette products advertised as exempt from regulation. It is unknown whether the use of 6MN has spread to other product categories. Industry reports, patent and trademark databases were searched for 6-methyl nicotine products. Identified trademarks 'Metatine', 'Nixotine', 'Imotine' were used to search for US-marketed products. Ingredient information was compared to US market-leading products, and safety warnings and regulatory statements were assessed in context with US state and federal regulations. Two US-based oral pouch brands, 'MG' and 'Hippotine' pouches, were identified in August 2024, advertised to contain 'Imotine'-trademarked 6MN. MG Pouches are marketed in four flavors, and 'Hippotine'-branded pouches are marketed in two flavors, likely representing banned flavor characterization in US state jurisdictions such as California. 6MN contents range 8-25 mg. Otherwise, the ingredient lists were almost identical across both product lines. Products list extensive addiction and health warnings, including warnings not to operate vehicles. Vendors state that these are not tobacco products, which implies that federal and state tobacco regulations do not apply. The spread of nicotine analogs to additional product categories, such as oral pouches, is concerning, especially given the high declared 6MN contents exceeding nicotine in popular US-marketed oral nicotine pouch products. Legislators and regulators need to provide certainty about the regulatory status of nicotine analogs to prevent further erosion of tobacco flavor bans and other regulations.

Life cycle assessment (LCA) of biodegradable linear low-density polyethylene (LLDPE) manufactured in India

The global plastic crisis requires urgent attention, as highlighted by a comprehensive review of LCA studies on plastics. Mismanagement of plastic waste has worsened this crisis, affecting all life forms and contaminating natural resources. Plastic production leads to Green House Gas (GHG) emissions. Plastic consumption has resulted in pollution across land, water, and even the air, with microplastics entering the human posing health risks like cancer and allergies. The article stresses the importance of LLDPE and explores the potential of bioplastics. Identifying research gaps led to the initiation of an innovative cradle-to-grave LCA investigation. NICHEM Solutions pioneered a three-stage process to manufacture three products: 1) a novel additive, 2) biodegradable LLDPE granules from the additive, and 3) biodegradable LLDPE film from these granules. Utilizing GaBi software and adhering to ISO 14040 guidelines, this study assessed the environmental and health impacts associated with all three products. Additive production had the highest environmental impact, followed by granule and film formation. Material production led to the overall environmental footprint, with LLDPE film production exhibiting notable effects. Transportation impacts were minimal but presented opportunities for optimization. Results revealed significant marine aquatic impacts across various processes, with global warming and human toxicity also identified as concerns. The study also evaluated alternative energy sources, finding natural gas to be the most favorable overall, followed by solar energy and biogas. Conversely, coal and grid-based electricity usage had the most detrimental effects on global warming and marine aquatic impact. Disposal methods such as landfilling and incineration were shown to exacerbate environmental impacts across multiple categories.

Analysis of the Urine Volatilome of COVID-19 Patients and the Possible Metabolic Alterations Produced by the Disease.

Alterations in metabolism caused by SARS-CoV-2 infection have been highlighted in various investigations and have been used to search for biomarkers in different biological matrices. However, the selected biomarkers vary greatly across studies. Our objective is to provide a robust selection of biomarkers, including results from different sample treatments in the analysis of volatile organic compounds (VOCs) present in urine samples from patients with COVID-19. Between September 2021 and May 2022, urine samples were collected from 35 hospitalized COVID-19 patients and 32 healthy controls. The samples were analyzed by headspace (HS) solid phase microextraction (SPME) coupled to gas chromatography-mass spectrometry (GC-MS). Analyses were conducted on untreated urine samples and on samples that underwent specific pretreatments: lyophilization and treatment with sulfuric acid. Partial Least Squares Linear Discriminant Analysis (PLS-LDA) and Subwindow Permutation Analysis (SPA) models were established to distinguish patterns between COVID-19 patients and healthy controls. The results identify compounds that are present in different proportions in urine samples from COVID-19 patients compared to those from healthy individuals. Analysis of urine samples using HS-SPME-GC-MS reveals differences between COVID-19 patients and healthy individuals. These differences are more pronounced when methods that enhance VOC formation are used. However, these pretreatments can cause reactions between sample components, creating additional products or removing compounds, so biomarker selection could be altered. Therefore, using a combination of methods may be more informative when evaluating metabolic alterations caused by viral infections and would allow for a better selection of biomarkers.

Use of carbon-14 labeled trichloroethene to assess degradation potential in rock core microcosms

In fractured rock aquifers contaminated with trichloroethene (TCE), the extent of groundwater plumes is impacted by degradation occurring within the rock matrix. The objective of this study was to evaluate TCE degradation in rock samples from three sites where in situ conditions may favor natural or enhanced attenuation. Intact rock core microcosms (94 total) were used to assess in situ conditions and enhancement by addition of lactate or lactate + sulfate. A key advance for this study was inclusion of carbon-14 (14C) labeled TCE in the experimental design, which enables monitoring of 14C-labeled products in addition to more readily detectable compounds associated with TCE degradation (i.e., cis-1,2-dichloroethene (cDCE), vinyl chloride, acetylene, ethene, and ethane). 14C-labeled products comprised 35–95 % of the total degradation products recovered over 9–21 months of monitoring, indicating that inclusion of 14C-TCE was essential to capturing the full potential for abiotic and biotic degradation of TCE. Microcosms infused with TCE but not 14C-TCE exhibited enrichment in δ13C-TCE, and enrichment in δ13C-cDCE in microcosms that underwent reductive dechlorination of TCE to cDCE. The results demonstrate the advantages of using diffusion-transport microcosms and 14C-TCE to document degradation of chlorinated ethenes in fractured rock aquifers.

Strategy for Optimizing Vitamin B12 Production in Pseudomonas putida KT2440 Using Metabolic Modeling.

Background/Objectives: Vitamin B12 is very important for human health, as it is a cofactor for enzymatic activities and plays various roles in human physiology. It is highly valued in the pharmaceutical, food, and additive production industries. Some of the bacteria currently used for the vitamin production are difficult to modify with gene-editing tools and may have slow growth. We propose the use of the bacteria Pseudomonas putida KT2440 for the production of vitamin B12 because it has a robust chassis for genetic modifications. The present wok evaluates P. putida KT2440 as a host for vitamin B12 production and explore potential gene-editing optimization strategies. Methods: We curated and modified a genome-scale metabolic model of Pseudomonas putida KT2440 and evaluated different strategies to optimize vitamin B12 production using the knockin and OptGene algorithms from the COBRA Toolbox. Furthermore, we examined the presence of riboswitches as cis-regulatory elements and calculated theoretical biomass growth yields and vitamin B12 production using a flux balance analysis (FBA). Results: According to the flux balance analysis of P. putida KT2440 under culture conditions, the biomass production values could reach 1.802 gDW-1·h1·L-1, and vitamin B12 production could reach 0.359 µmol·gDW-1·h-1·L-1. The theoretical vitamin B12 synthesis rate calculated using P. putida KT2040 with two additional reactions was 14 times higher than that calculated using the control, Pseudomonas denitrificans, which has been used for the industrial production of this vitamin. Conclusions: We propose that, with the addition of aminopropanol linker genes and the modification of riboswitches, P. putida KT2440 may become a suitable host for the industrial production of vitamin B12.

THE PROBLEM OF PROFESSIONALLY SIGNIFICANT EXPERIENCE IN CONTEMPORARY PSYCHOLOGY

It was proved in the article, that among the students of experimental and control groups, before the experimental training, the most important instrumental values were: high demands, cheerfulness, independence, accuracy, education, conscientiousness in the execution of assignments. Unfortunately, such important instrumental values for the future psychologist as sensitivity, tolerance for the opinions of others, the ability to make thoughtful decisions, self-control and others have a rather low level of formation (for example, sensitivity; tolerance of others’ opinions). According to the results of our research, individual experience is actualized during the formation of the subject, the so-called “going out to meet something”. This is explained by the fact that our self-realization, although important in itself, is optional, an additional product of our desire, that is, in other words, a human calling that inspires a person to perform actions. In this context we’d remark on subjective experience (“awareness”), which is a component of a real dialogue is quite important. We call another danger from the person’s focus on his/her personality “hidden spontaneity”. This spontaneity is hidden because we want to become spontaneous, we set this as our goal, but then we try to structure this spontaneity. Such a process takes place during encounter groups. However, we point out, the danger is that what worked for one person in one situation may not necessarily work in another situation, so transference should not be applied in every case. But, as a rule, at the moment when a person becomes experienced, then he/she wants to transfer this experience to other subjects. As a result of this, the emergence of authoritarianism on the part of the coach during the group meetings classes is possible. In the context of the analysis of the experience that we carry out, one can see the efforts to overcome the methodology of simplified determinism. Thus, Humanistic Psychology paradoxically comes close to weakening its own subjective idea. Directly for the results of our research, the key point in our theory was the construction of a universal scale of needs from lower to higher, which differs from the levels of the formation or functioning of needs in representatives of behavioral psychology or known levels of psychoanalysis, in their essence, only an instruction to look for the key to anatomy not from below, but from above. The heuristic nature of the classification and ranking of human needs in the operational-procedural plan is not in doubt, but, nevertheless, objections arise when even any broad normalization of human actualizations enters the limits of the methodological paradigm. It is difficult to say to what extent such regulation is unhumanistic, but it is clear that to a large extent it appears as undemocratic.

Atomistic simulation of primary microstructure formation in metals during crystallization from the melt

Additive manufacturing of metallic parts by Selective Laser Melting (SLM) implies high temperature gradients and small volume of the melt bath. These conditions make the process scales close to those available for state-of-the-art massively parallel atomistic simulations. In the paper, the microscopic mechanisms responsible for the formation of primary microstructure during molten metal solidification are investigated using classical molecular dynamics (CMD). The 316L austenitic stainless steel with face centred cubic lattice, which is widely used in industry including SLM applications was chosen as a material for the CMD simulations. It was shown that solidified material inherits substrate defects and catches new ones, which interact with the solidification front thus producing the primary microstructure. Peculiarities of solidification in different crystallographic directions and solidification front interaction with grain boundaries and newly produced defects (mostly twin boundaries) as well as their formation are under study. Resulting microstructures of virtual samples are compared with those of real samples produced by SLM and analysed by the electron backscatter diffraction (EBSD) method. The comparison shows similarities of EBSD and CMD sample patterns and evidences for the capability of the large-scale atomistic simulations to reproduce main features of the microstructures formed in the metallic SLM additive production.

Reactions of Diphenylamine with OH Radicals in the Environment: Theoretical Insights into the Mechanism, Kinetics, Temperature, and pH Effects.

Diphenylamine (DPL) has been widely utilized in industrial chemicals, but its degradation by HO• radicals in the environment has not been fully studied yet. The present study uses quantum chemical calculations to evaluate the reaction of DPL with HO• radicals in atmospheric and aqueous environments. The results showed that, in the atmosphere, the diphenylamine reacted with the HO• radical rapidly, with an overall rate constant of 9.24 × 1011 to 1.34 × 1011 M-1 s-1 and a lifetime of 0.17 to 1.55 h at 253-323 K. The calculated overall rate constant in water (koverall = 1.95 × 1010 M-1 s-1, pH = 3-14) is in excellent agreement with the experimental value (koverall = 1.00 × 1010-1.36 × 1010 M-1 s-1). The HO• + DPL reaction in water could occur following the hydrogen transfer (15.4%), single electron transfer (41.6%), and radical adduct formation (41.7%) mechanisms, clarifying that addition products were not exclusive products. Nevertheless, variations in temperature and pH within aqueous environments had an impact on the mechanisms, kinetics, and degradation products of the reaction of DPL with HO• radicals.

The metabolic engineering of Escherichia coli for the high-yield production of hypoxanthine

BackgroundHypoxanthine, prevalent in animals and plants, is used in the production of food additives, nucleoside antiviral drugs, and disease diagnosis. Current biological fermentation methods synthesize quantities insufficient to meet industrial demands. Therefore, this study aimed to develop a strain capable of industrial-scale production of hypoxanthine.ResultsDe novo synthesis of hypoxanthine was achieved by blocking the hypoxanthine decomposition pathway, thus alleviating transcriptional repression and multiple feedback inhibition, and introducing a purine operon from Bacillus subtilis to construct a chassis strain. The effects of knocking out the IMP(Inosine 5’-monophosphate) branch on the growth status and titer of the strain were then investigated, and the effectiveness of adenosine deaminase and adenine deaminase was verified. Overexpressing these enzymes created a dual pathway for hypoxanthine synthesis, enhancing the metabolic flow of hypoxanthine synthesis and preventing auxotrophic strain formation. Introducing IMP-specific 5’ -nucleotidase addressed the issue of adenylate accumulation. In addition, the metabolic flow of the guanine branch was dynamically regulated by the guaB gene. The supply of glutamine and aspartic acid precursors was enhanced by introducing an exogenous glnA mutant gene, overexpressing aspC, and replacing the weaker promoter to regulate the aspartic acid branching pathway. Ultimately, fermentation in a 5 L bioreactor for 48 h produced 30.6 g/L hypoxanthine, with a maximum real-time productivity of 1.4 g/L/h, the highest value of hypoxanthine production by microbial fermentation reported so far.ConclusionsThe intracellular purine biosynthesis pathway is extensive and regulated at multiple levels in cells. The IMP branch in the hypoxanthine synthesis pathway has a higher metabolic flux. The current challenge lies in systematically allocating the metabolic flux within the branch pathway to achieve substantial product accumulation. In this study, E. coli was used as the chassis strain to construct a dual pathway for IMP and AMP(Adenosine 5’-monophosphate) synergistic hypoxanthine synthesis and dynamically regulate the guanine branch pathway. Overall, our experimental efforts culminated in a high-yield, plasmid- and defect-free engineered hypoxanthine strain.

Computer big data technology in additive manufacturing and product design in sustainable manufacturing

Computer big data technology in additive manufacturing and product design in sustainable manufacturing

Synthesis of Os Hydride Complexes Supported by the Diarylamido/Bis(phosphine) PNP Ligand and Attempts at Using (PNP)Ru and (PNP)Os Complexes in C-H Borylation Catalysis.

This manuscript describes the synthesis of Os complexes supported by the diarylamido/bis(phosphine) PNP pincer ligand. Compound (PNP)OsH(CO) (3-Os) was prepared by analogy with the previously reported 3-Ru. However, attempts to make (PNP)OsH3 (4-Os) analogously to 4-Ru resulted in the formation of an unexpected compound (5-Os) that is a product of addition of a BH3 unit across the Os-N bond in 4-Os. Nonetheless, 4-Os was prepared via an alternative route. Unlike 4-Ru, 4-Os appears to be a classical trihydride. Compounds 3-Ru, 3-Os, 4-Os, 4-Ru, and 5-Os were tested as potential catalysts for (a) dehydrogenative borylation of terminal alkynes (DHBTA) and (b) dehydrogenative borylation of benzene. No catalytic C-H borylation was observed for any of them, but all of them catalyzed unselective hydroboration of 4-MeC6H4CCH.

ESTIMATION OF THE CLEANING TIME OF THE BOTTOM-HOLE ZONE OF WELLS IN CONDITIONS OF UNCERTAINTY

The aim of the work is to analyze changes in the maximum optimal flow rates of wells operating various groups of productive formations of the Volga-Ural oil and gas province under conditions of uncertainty of geological and field data. The relevance of the work is due to the deterioration of the structure of residual oil reserves and the need for the most efficient extraction of liquid hydrocarbon resources, optimization of design methods for various treatments of the bottomhole formation zone. Using geological and statistical modeling systems, the timing of cleaning the bottomhole zones of wells from drilling products was determined at the time they reached their maximum optimal flow rate in conditions of deposits confined to various stratigraphic horizons, and appropriate models of changes in productivity coefficients were constructed. The factors influencing the recovery time of the real properties of the reservoir at the point of opening it by the well, which, among other things, determines the profitability of the process of selecting residual oil reserves, have been established. Among them, the most relevant and necessary for the implementation of an integrated approach to computer support procedures for the development of oil fields are the productivity of wells, fracturing of formations, their proximity to a particular tectonic-stratigraphic complex, geological, physical and physico-chemical properties of formations and their saturating fluids. For the deposits of the Famensk tier of deposits of the Volga-Ural oil and gas province, a multiple decrease in the time of removal of reaction products after hydrochloric acid exposure at objects with low fracturing has been established. At the same time, additional processing of the bottomhole formation zone within areas with high drilling density does not affect the total cleaning time of the bottomhole zone of wells. Stimulation of the removal of drilling mud filtrate and additional products of formation reactions by injection of hydrochloric acid compositions under certain conditions can lead to a decrease in the actual productivity of wells due to the introduction of less developed and dead-end cracks into the drainage process. The existence of the specifics of the procedure for cleaning the bottomhole formation zone depending on the geological and physical parameters of productive formations is substantiated and various patterns of changes in the productivity coefficient depending on the time of wells reaching the maximum optimal flow rate are revealed. The results obtained make it possible to make informed technological decisions to improve the efficiency of oil production, including in conditions of uncertainty, which may be caused by the lack of the necessary volume of hydrodynamic studies of wells.

Gold‐Catalyzed Annulation Between 1‐[2‐(Dimethoxymethyl)phenyl]prop‐2‐yn‐1‐yl Acetates and α‐Diazo Esters or Anilines to Form Substituted Naphthoates and Indeno[1,2‐b]quinoline, Respectively

AbstractThis work describes gold‐catalyzed annulations of 1‐(2‐(dimethoxymethyl)phenyl)‐1‐prop‐2‐yn‐1‐yl acetate substrates with α‐diazo esters and anilines, respectively yielding substituted naphthoates and indeno[1,2‐b]quinoline derivatives. Our mechanistic analysis indicates 1‐methoxy‐2‐carbonyl‐1H‐indenes as their common intermediates. In the formation of naphthoates, α‐diazo ester attacks at gold‐bound intermediate via an SN2 pathway to enable a allylic methoxy substitution. For indeno[1,2‐b]quinoline derivatives, there are two main reactions, including (i) a reversible formation between intermediate and a double amine addition product and (ii) an arylation reaction of intermediate. Species N‐(phenyl((2E)‐1‐(phenylimino)‐1,3‐dihydro‐2H‐inden‐2‐ylidene)methyl)aniline was converted to indeno[1,2‐b]quinoline in the presence of gold catalyst.

Within‐canopy carbon partitioning to cotton leaves in response to irrigation

AbstractWhen water resources are limited, cotton (Gossypium hirsutum L.) plants adapt in part through adjustments in carbon allocation strategies, often evident in the leaves within the canopy. The dynamics of leaf carbon accumulation provide insights into how the plant partitions and uses carbon resources, a key aspect of optimizing crop productivity. In this study, we investigated the dynamics of leaf carbon accumulation in two cotton cultivars (Phytogen [PHY] 350 and Stoneville [ST] 5707) across spatial and temporal scales under two different levels of irrigation (low: 178 mm year−1, high: 356 mm year−1) in 2020 and 2021. For each cultivar and irrigation treatment, an increase in leaf mass occurred primarily at the bottom of the canopy early in the season, followed by additional leaf production in the middle of the plant as the season progressed. Irrigation reduction resulted in a canopy with reduced radiation interception, less leaf shading in the lower canopy, and thicker leaves. In contrast, more irrigation created canopies with a larger effective leaf area, increasing total light interception despite increased shading at the canopy base. Additionally, leaf carbon allocation is synchronized with fruit carbon demand at the onset of the first bloom stage for an early‐maturing cultivar. Overall, this study provides valuable insights into the complex relationship between water availability, radiation intensity within the canopy, and leaf carbon dynamics, contributing to a more comprehensive understanding of the plant's overall performance in resource‐constrained environments.

Exposure of RAW264.7 macrophages to exhaust emissions (gases and PAH) and non-exhaust emissions (tire particles) induces additive or synergistic TNF-α production depending on the tire particle size

Road traffic is a major contributor to air pollution and consequently negatively affects human health. Car pollution originates both from exhaust emissions (EE) and non-exhaust emissions (NEE, such as tire and brake wear particles, erosion of road surfaces and resuspension of road dust). While the toxicity of EE and NEE has been characterized separately, their combined effects are poorly documented. However, we are constantly exposed to a mixture of pollutants and their interactions should not be neglected as they may significantly impact their toxicological profile resulting in additive, synergistic or antagonistic effects. To fill this gap, we investigated in vitro the combined toxicity of exhaust gases and benzo[a]pyrene (representative of EE) and tire particles (representative of NEE). Macrophages from the RAW264.7 cell line were exposed for 24 h to tire particles (TP) of variable size (6–113 µm), alone or in combination with exhaust gases (CO2, CO, NO, NO2) and benzo[a]pyrene (B[a]P) as an archetype of polycyclic aromatic hydrocarbon (PAH). The cell response was assessed in terms of cytotoxicity, proinflammatory response and oxidative stress. TP, gases and B[a]P, alone or in combination triggered neither cytotoxicity nor oxidative stress. On the contrary, a proinflammatory response was elicited with two different profiles depending on the size of the TP: TNF-α production was either slightly (with the finest TP) or strongly (with coarse TP) increased in the presence of gases and B[a]P, suggesting that the effects of TP, gases and B[a]P were either additive or synergistic, depending on TP size.

Human papillomavirus prophylactic vaccines: update on new vaccine development and implications for single-dose policy.

Human papillomavirus (HPV) prophylactic vaccines were first licensed in 2006 with the primary goal of preventing HPV-related cancers, with cervical cancer accounting for the highest morbidity and mortality globally. Six HPV vaccines have been licensed; 4 of these have been prequalified by the World Health Organization, and additional products are in the pipeline. This article provides an overview of HPV vaccine coverage and current and anticipated vaccine supply vs expected demand. Given that the 2022 World Health Organization position paper on HPV vaccines includes a 1-dose regimen as an alternate schedule, we will discuss the evidence for using licensed vaccines in single-dose regimens and the approach to generating similar supportive data for other current and future vaccines. The broad adoption of a single-dose HPV vaccine regimen would expand access to vaccines by improving the supply-demand balance, increasing affordability, and simplifying logistics, which will ultimately impact HPV-related morbidity and mortality.