Salt‐affected soils have low biological activity both because of osmotic and ionic effects of salts and owing to ¡imitation of carbonaceous substrates. Alkali soils and artificially salinized soils were incubated at 28°C for 3 months with or without organic (1% Sesbania cannabina green manure) amendment, at 60% water‐holding capacity. CO2 evolution was measured by absorption in alkali, microbial biomass by chloroform fumigation‐incubation, dehydrogenase activity by tetrazolium reduction, and urease activity by measuring ammonia release titrimetrically. CO2 evolution and microbial biomass were reduced at pH 8.7 in unamended alkali soils by 18% and 35%, respectively; at pH 10.3 the reduction was by 83% and 35%, respectively. Organic matter (OM) amendment stimulated CO2 evolution, and the biological activity in soils up to pH 10.0 was restored to that of amended normal soil (pH 8.1). The activity of pH 10.3 soil was 78% of normal. Extent of added carbon decomposed was ∼38% throughout the pH range studied. CO2 evolution and microbial biomass were reduced at ECe 16 in unamended saline soils by 33% and 40%, respectively; at ECe 97 it was reduced by 62% and 63%, respectively. OM amendment improved activity only at salinities ECe ≤ 26. Dehydrogenase activity (DHA) showed a similar trend; OM stimulated activity in alkali soils up to pH 10.0, whereas in saline soils it could not fully restore the activity even at ECe 16.0. In saline soils there was an immediate reduction in DHA on salt addition followed by a further reduction on incubation. DHA increased immediately on salt removal by leaching. There was a reduction in pH and ESP values and increases in soil C and N contents of alkali soils, and an increase in urease activity of saline and alkali soils following the addition of organic amendment. Results supported our hypothesis that microbial growth is depressed in alkali soils owing, at least in part, to carbon stress and in saline soils owing to salt stress. Results reinforce the need to apply organic amendments during alkali soil reclamation, whereas in saline soils, organic amendments must follow leaching.