Addition Of Inoculum Research Articles

Co-digestion of Pretreated Chicken – Goat and Untreated Cow Manure at Different Substrate to Inoculums Ratios and Total Solids for Biogas Production

Biogas production can be greatly affected by inoculum addition and total solids. The effect of the substrate to inoculum ratios and total solids of chicken, goat and cow manure on biogas production was studied using a 0.15m3 laboratory-scale batch digester at a constant temperature of 35°C. Feedstocks were mechanically minced to 3 mm effective particle sizes prior to co-digesting with untreated cow manure from a free-range dairy rearing system. Different amounts of cow substrate inoculum were used at ratios of 2:1, 3:1, 4:1, 5:1 and 6:1, while total solid levels between (7.5% and 10.5%) at intervals of 0.5% were used to study their effects on biogas production. Increasing inoculums and total solids resulted in increased biogas production with peaks at a substrate to inoculum ratio of 4:1 (20% inoculum addition) and 9% total solids. Biogas production rates of 0.61 and 0.63m3/m3d were realized respectively. Keywords: Biogas Production, Chicken-Goat-Cow Manure, Substrate to Inoculum Ratios, Total Solids

Co-digestion of Pretreated Chicken – Goat and Untreated Cow Manure at Different Substrate to Inoculums Ratios and Total Solids for Biogas Production

Biogas production can be greatly affected by inoculum addition and total solids. The effect of substrate to inoculum ratios and total solids of chicken, goat and cow manure on biogas production was studied using a 0.15m3 laboratory scale batch digester at a constant temperature of 35°C. Feed stocks were mechanically minced to 3 mm effective particle sizes prior to co-digesting with untreated cow manure from a free-range dairy rearing system. Different amounts of cow substrate inoculum were used at ratios of 2:1, 3:1, 4:1, 5:1 and 6:1, while total solid levels between (7.5% and 10.5%) at intervals of 0.5% were used to study their effects on biogas production. Increasing inoculums and total solids resulted in increased biogas production with peaks at a substrate to inoculum ratio of 4:1 (20% inoculum addition) and 9% total solids. Biogas production rates of 0.61 and 0.63m3/m3d were realized respectively. Keywords: Biogas Production, Chicken-Goat-Cow Manure, Substrate to Inoculum Ratios, Total Solids

In-depth study to decipher mechanisms underlying Arabidopsis thaliana tolerance to metal(loid) soil contamination in association with biochar and/or bacteria

Metal(loid)s are toxic to animal life, human health and plants; therefore, their removal from polluted areas is imperative in order to minimize their impact on the ecosystems. The use of plant-amendment-microorganism synergy is a promising option, but not yet fully explored, to manage lands contaminated with metal(loid)s. However, molecular factors and mechanisms underlying this interaction are almost unknown. The aim of the present study was to evaluate the effects of amendments and bacteria, both alone and in combination, on Arabidopsis thaliana grown on arsenic and lead polluted soils. To accomplish this aim, a pot experiment was performed testing the effect of biochar and/or autochthonous metal(loid) resistant Bacillus isolates on physico-chemical soil properties and on plant growth and metal(loid) uptake/intake. Furthermore, bioinformatics-assisted proteomics was used to understand common and specific mechanisms regulating plant growth and metal(loid) tolerance in tested conditions. Results showed that biochar and/or Bacillus induced significant and positive effects on soil properties, increasing pH, Ctot, Ntot and Ptot concentrations and decreasing nutrients (Nav and Pav), As and Pb availability. Plant growth was also enhanced by addition of biochar and/or bacterial inoculum, reaching the maximum when biochar and microorganism were combined. The deciphering of molecular mechanisms revealed that combination of biochar and bacterial inoculation mitigate Arabidopsis growth and defense tradeoff, and underline the great potential of plant-biochar-inoculum synergic application in more effective and large scale-up phytostabilizing systems.

Membrane bioreactor-assisted volatile fatty acids production and in situ recovery from cow manure

Cow manure (CM) generation in large volumes has for long been considered a waste management challenge. However, the organic content of CM signals opportunities for the production of value-added bioproducts such as volatile fatty acids (VFAs) through anaerobic digestion (AD). However, a robust VFAs fermentation process requires effective methane formation inhibition and enhance VFAs recovery. In this study, thermal pretreatment was applied to inhibit methanogens for enhanced VFAs production and an immersed membrane bioreactor (iMBR) for in situ recovery of VFAs in a semi-continuous AD. Maximal VFAs yield of 0.41 g VFAs/g volatile solids (VS) was obtained from thermally-treated CM without inoculum addition. The CM was further fed to the iMBR operating at organic loading rates of 0.8–4.7 gVS/L.d. The VFAs concentration increased to 6.93 g/L by rising substrate loading to 4.7 g VS/L.d. The applied iMBR set-up was successfully used for stable long-term (114 days) VFAs production and recovery.

Potential Control of Mycotoxigenic Fungi and Ochratoxin A in Stored Coffee Using Gaseous Ozone Treatment

The objective of this study was to examine the effect of treatment of Arabica green coffee beans with gaseous ozone (O3) for the control of ochratoxigenic fungi and ochratoxin A (OTA) contamination by Aspergillus westerdijkiae, A. ochraceus, and A. carbonarius during storage. Studies included (i) relative control of the populations of each of these three species when inoculated on irradiated green coffee beans of different initial water availabilities using 400 and 600 ppm gaseous O3 treatment for 60 min at a flow rate of 6 L−1 and on OTA contamination after 12 days storage at 30 °C and (ii) effect of 600 ppm O3 treatment on natural populations of green stored coffee beans at 0.75, 0.90, and 0.95 water activity (aw) or with additional inoculum of a mixture of these three ochratoxigenic fungi after treatment and storage for 12 days at 30 °C on fungal populations and OTA contamination. Exposure to 400 and 600 ppm O3 of coffee beans inoculated with the toxigenic species showed that there was less effect on fungal populations at the lowered aw (0.75). However, toxigenic fungal populations significantly increased 48 h after exposure and when stored at 0.90 and 0.95 aw for 12 days. All three species produced high amounts of OTA in both O3 treatments of the wetter coffee beans at 0.90 and 0.95 aw. Gaseous O3 (600 ppm) treatment of naturally contaminated green coffee beans had little effect on fungal populations after treatment, regardless of the initial aw level. However, after storage, there was some reduction (26%) observed in coffee at 0.95 aw. In addition, no fungal populations or OTA contamination occurred in the 0.75 and 0.90 aw treatments after exposure to 600 ppm gaseous O3 and storage for 12 days. It appears that under wetter conditions (≥0.90–95 aw) it is unlikely that fungal populations and OTA contamination of stored coffee beans, even with such high O3 concentrations would be controlled. The results are discussed in the context of potential application of O3 as an intervention system for stored coffee post-fermentation and during medium term storage and transport.

Biological denitrification potential as an indicator for measuring digestate stability

Biological stability is an essential parameter for assessing the environmental impact from the land application of digestate as organic amendment. In this paper, a new indicator, biological denitrification potential (BDP), was developed for evaluating the biological stability of digestate. Digestate samples collected along the digestion process from a mesophilic anaerobic batch digester fed with food waste were investigated under different solid retention time. The value of BDP based on nitrate removal ranged from 176.3 to 48.3 mg-N/g-VSdigestate, corresponding well to the digestion time, and strongly correlated with total organic carbon content. Evolution trends similar to respiration index (RI) and biochemical methane potential (BMP) can be also observed for BDP, indicating that values presented of these stability indices decreased with the degree of digestate stabilization. The mass balance of the BDP process indicated that nitrate was mainly converted into N2 gas with mineralizing organic carbon from digestate, implying that biostability evaluated by BDP depends on carbon source and denitrification activity in digestate. The denitrifying bacteria Thiopseudomonas and Pseudomonas accounted for the majority of microorganisms. These findings of this study concluded that BDP can be an efficient indicator to assess the bio-stability of digestate planned for agricultural or land use. Compared with the existing biostability index, BDP has the additional advantage of no exogenous inoculum addition, homogenous test condition and possibility of shortening incubation time.

Material Characterization and Substrate Suitability Assessment of Chicken Manure for Dry Batch Anaerobic Digestion Processes.

Chicken manure is an agricultural residue material with a high biomass potential. The energetical utilization of this feedstock via anaerobic digestion is an interesting waste treatment option. One waste treatment technology most appropriate for the treatment of stackable (non-free-flowing) dry organic waste materials is the dry batch anaerobic digestion process. The aim of this study was to evaluate the substrate suitability of chicken manure from various sources as feedstock for percolation processes. Chicken manure samples from different housing forms were investigated for their chemical and physical material properties, such as feedstock composition, permeability under compaction and material compressibility. The permeability under compaction of chicken manure ranged from impermeable to sufficiently permeable depending on the type of chicken housing, manure age and bedding material used. Porous materials, such as straw and woodchips, were successfully tested as substrate additives with the ability to enhance material mixture properties to yield superior permeability and allow sufficient percolation. In dry anaerobic batch digestion trials at lab scale, the biogas generation of chicken manure with and without any structure material addition was investigated. Digestion trials were carried out without solid inoculum addition and secondary methanization of volatile components. The specific methane yield of dry chicken manure was measured and found to be 120 to 145 mL/g volatile solids (VS) and 70 to 75 mL/g fresh matter (FM), which represents approximately 70% of the methane potential based on fresh mass of common energy crops, such as corn silage.

EFEKTIVITAS Glomus clorum TERHADAP PERTUMBUHAN TANAMAN CABAI RAWIT (Capsicum frutescens L.), TOMAT (Solanum lycopersicum L.) DAN TERUNG (Solanum melongena L.)

Increasing plant growth by arbuscula mycorrhizal fungi depends on the fungus and plant spesies. The aim of this study was to determine the efectivity of fungal mycorrhizal fungus on the growth of cayenne pepper, tomato and eggplant which are Solanaceae family members. The study was conducted by Completely Randomized Design method.with two factors. The fisrt factor was species of experimental plant consisted of cayenne pepper (Capsicum frutescens L.), tomato (Solanum lycopersicum L.) and eggplant (Solanum melongena L.). The second factor was addition of Glomus clorum inokulum consisted of with and without inoculum addition. The result showed that there was no effect of inoculum addition on plant growth of three experiemal plants. The quality of inoculum used for this experiment was not good enough furthermore fungi could not germinate and colonize plant root.

PERTUMBUHAN BAWANG MERAH (Allium cepa L.) YANG DIBERI PUPUK KOMPOS CAIR DAN JAMUR MIKORIZA ARBUSKULAR

The application of liquid compost and of beneficial soil microorganism such as arbsucular mycorrhizal fungi can be used to replace chemical fertilizer application. The aim of this study was to investigate the growth of red onion (Allium cepa L.) plant fertilized by liquid compost and inoculated by arbuscular mycorrhizal fungi. This study was conducted based on a completely randomized design with two factors. The first factor was an addition of liquid compost dosages (0, 50, 100 and 200 ml/polybag). The second factor was an addition of AM fungal inoculum (with and without addition). The results showed that the growth of red onion plant was not significantly affected by the addition of liquid compost and AM fungal inoculum. However, the red onion plant fertilized by 200 ml liquid compost per polybag had the lowest shoot dry weight. The addition of AM fungal inoculum was not increasing plant growth because the quality of used inoculum was not good.

Optimizing Photoperiodism, Growth Media Dilution, and Frequency of Inoculum Addition in Microalgae (Chlorella vulgaris) Culture Using Bi-oslurry (ADDMW) as Growth Media

In biogas production using dairy manure, an anaerobic digestion process also produced a by product bioslurry, also known as Anaerobically Digested Dairy Manure Wastewater (ADDMW), which is rich in ammonium and ortophosphate. ADDMW is potentially to be used as growth media to grow autotrophic organism, including microalgae. Furthermore, microalgae potential-ly could reduce organic content in ADDMW, thus play a role as phycoremediator to organic wastewater. In this study we used ADDMW medium for the growth of microalgae, determining its growth kinetics and level of reduction in ammonium and or-thophosphate content in ADDMW. In addition, we also analysed protein content in microalgae biomass for potential use as animal feed. The experiment was conducted using some variations in photoperiodism, i.e., 16:8; 12:12; and 8:16 and medium dilution factor of 2.5 and 5 times. Furthermore, frequency of inoculum addition was also examined i.e., a) 80 mL on day 0 (once); b) 26.67 mL on day 0 to 2 (first 3 days); and c) 8 mL on days 0 to 9 (every day). The results showed that 16:8 photo-period and 5 times medium dilution produced the highest biomass growth and productivity kinetics. Variation in frequency of inoculum addition showed no significant effect to a decrease in ammonium levels but indicated some effect to orthophosphate level. It can be concluded that microalgae can be grown in ADDMW media and can reduce its ammonium and ortophosphate content, as a phycoremediator, before the wastewater is dumped to the river.

PENINGKATAN PERTUMBUHAN TANAMAN JAGUNG ( Zea mays L.) MENGGUNAKAN JAMUR MIKORIZA ARBUSKULAR DARI JENIS YANG BERBEDA PADA KONDISI CEKAMAN AIR

Arbsucular mycorrhizal fungi promote plant growth by enhancing mineral uptake. Contribution degree of arbuscular mycorrhizal fungi to promote plant growth depend on species of plant-fungus association. The aim of this study was to compare the ability of three species of Glomus to promote maize plant growth. Maize plants were inoculated with 20 g inoculum of either Glomus deserticola, Glomus etunicatum, or Glomus clorum. Inoculum was soil containing spore, hyphae and infected root. Maize plants without addition inoculum were also used as a control. Water availability in the soil as growing medium was maintained on 40% field capacity. The results showed that addition of inoculum from three species of Glomus increased average of maize plant shoot dry weight although there was no statisticaly significant differences. Maize plant inoculated with G. clorum had higher shoot dry weight than maize plant inoculated either with G. etunicatum or G. deserticola while root colonization by G. clorum was lowest.

Fermentasi Kitin dari Limbah Cangkang Kepiting Menggunakan Jamur Rhizopus Oryzae pada Berbagai Kadar Air

Chitin on crab shell waste can be obtained by fermentation process use Rhizopus oryzae. The objective of this research are to determine the optimum of water content on crab shell waste fermentation by Rhizopus oryzae for chitin production. Fermentation process is carried out for 72 hours, 5 of pH, 37oC, and 10% (v/w) of inoculum addition. Water content on fermentation process were varried whithin 40%, 50%, and 60% respectively. Fermentation process were carried out by 40% of water content reported as the optimum based on the deproteinization degree and the solubility in acidic solution.

Eco-compatible biochar mitigates volatile fatty acids stress in high load thermophilic solid-state anaerobic reactors treating agricultural waste

A high concentration of accumulated volatile fatty acids (VFAs) is one of the most important factors resulting in reactor failure during solid-state anaerobic digestion. In this study, the feedstock-to-inoculum (F/I) ratio (0.5, 2, 3, 4 and 6) and the recovery method after failure (biochar addition or inoculum addition) were investigated in batch solid-state anaerobic digestion fed with rice straw and pig urine. An F/I ratio of 3 was the threshold for stable operation, while the reactors failed at F/I ratios of 4 and 6 because of high accumulated VFAs concentrations (above 30 g HAc/kg). Biochar addition (10% or 20% (wet weight) of the mixture) was as effective as inoculum addition (by adjusting the F/I ratio to 2 or 3) in promoting VFAs degradation in failed reactors within a short period (<1 day). The buffering capacity of biochar was important in promoting VFAs degradation.

USE OF INOCULUM IN BIODIGESTERS WITH CATTLE MANURE UNDER CONVENTIONAL AND ORGANIC PRODUCTION SYSTEMS

ABSTRACT One way of making livestock farming a more sustainable practice is to raise cattle organically. However, regardless of the production system, there is a generation of residues that can be converted into energy resources using biodigesters. This study aimed to evaluate the addition of inoculum in the anaerobic biodigestion of cattle manure under organic (OPS) and conventional production systems (CPS) in an Indian model biodigester. The physicochemical (pH, EC, TS, VS, DBO, and COD) and microbiological characteristics of the affluent and effluent, as well as the production, potential, and qualitative characteristics of the biogas, were evaluated. CPS showed higher reductions in TS, VS, BOD, and COD than OPS, with the highest effect of biogas production. Regarding the effect of inoculum addition, the treatment with 20% inoculum provided in both production systems high acceleration at the starting point of biogas production and increased energy potential, as well as high reductions in VS, BOD, and COD. Thus, there is a need to use 20% inoculum for high biogas productivity, regardless of the production system.

Bioaugmented constructed wetlands for denitrification of saline wastewater: A boost for both microorganisms and plants

The inhibition of salt stress on plant and microbial functions has led to the reduction of nitrogen removal capacity of constructed wetlands (CWs) under saline conditions. The mechanisms and effectiveness of bioaugmented CW (Bio-CW) microcosms with a salt-tolerant microbial inoculum were evaluated for nitrogen removal at different salinity levels. The results showed that the denitrification capacity of CWs was improved under saline conditions by adding the salt-tolerant microbial inoculum. At an EC of 15 mS/cm, the removal percentages of ammonia nitrogen (NH4+-N) and total nitrogen (TN) in Bio-CW microcosms (95.7% and 99.4%) on Day 5 were significantly (p < 0.05) higher than that in unbioaugmented CW (un-Bio-CW) microcosms (68.5% and 76.4%), respectively. The high throughput sequencing data of substrate samples indicated that the microbial community in the CWs was changed by the addition of the salt-tolerant microbial inoculum and the frequency of bacteria with nitrogen removal function was increased in the CWs. Furthermore, both growth and the TN accumulation capacity of plants in Bio-CW microcosms were promoted compared with the un-Bio-CW microcosms. In conclusion, the addition of the salt-tolerant microbial inoculum can enhance the nitrogen removal efficiency of CWs under saline condition via boosting the function of both microorganisms and plants.

Giant bladder kelp (Macrocystis pyrifera) and maize (Zea mays) meals as nucleation sites for biofloc formation

The aim of this study was to evaluate Macrocystis pyrifera and Zea mays meals as nucleation sites in biofloc formation. Bioflocs were studied for a period of 12 weeks, by measuring volume, dry weight, organic matter, and ash weight. These were induced by the addition of bacterial inoculum, molasses and both types of meals to fresh water. After 4 weeks, a total of 100 tilapia fingerlings (1.60 ± 0.02 g weight and 4.76 ± 0.03 cm length) were transferred to 100 L tanks by triplicate for each treatment. Mean ± SD values of temperature (26.3 ± 0.2 °C), dissolved oxygen (3.42 ± .0.07 mg L−1), pH (7.95 ± 0.04), salinity (0.47 ± 0.01) and ammonia (0.245 ± 0.012 mg NH3 L−1) were similar in both treatments. However, floc volume of M. pyrifera was significantly higher (116.1 ± 20 ml L−1) than Z. mays flocs (58.4 ± 7.7 ml L−1), but had lower dry weight (55 ± 0.5 mg g−1 in M. pyrifera vs. 61 ± 2.0 mg g−1 in Z. mays). Fish weight and length gain and survival were significantly higher (p < 0.05) in M. pyrifera floc (13.7 ± 1.3 g; 4.5 cm; 90 %) than in Z. mays (12.3 g; 8.5 cm; 56 %). Aggregated organisms were classified into major groups and were quantified and identified to a genus level. Ciliates were detected on week 1 and rotifers and nematodes were first registered on weeks 3 and 4. These three groups were present in both treatments but were more abundant in maize biofloc. In conclusion, both types of nuclei resulted in the formation of bioflocs rich in aggregated organisms, but nuclei of M. pyrifera produced a significantly higher performance of tilapia fingerlings, probably because of the higher nutritional content of kelp over maize meal and higher flotation of M. pyrifera flocs, which remained longer in suspension making them readily available to the fish.

Inoculation and habitat amelioration efforts in biological soil crust recovery vary by desert and soil texture

As dryland degradation continues, it is increasingly important to understand how to effectively restore biocrust communities. Potential techniques include the addition of biocrust inoculum to accelerate biocrust recovery. Enhanced erosion typical of degraded environments creates a challenge for these approaches, due to loss by wind or water and burial by saltating particles. To retain and protect added inoculum, the inclusion of habitat‐amelioration techniques can improve recovery rates. This study tested three different types of inoculum (field‐collected, greenhouse‐cultivated, and laboratory‐cultivated biocrust) coupled with two treatments to augment soil stability and ameliorate habitat limitations: soil surface polyacrylamide additions and installation of straw barriers. This was done across two deserts (Great Basin and Chihuahuan) and separated into generally coarse‐ or finer‐textured soils in each desert, with results monitored for 3 years (2015, 2016, 2017). While the inoculum type, coupled with habitat ameliorations, occasionally enhanced biocrust growth across years and treatments, in other cases, it made no difference compared to natural recovery rates. Rather, the desert location and soil texture groupings were the most prominent factors in determining recovery trajectories. Recovery proportions were similar in the finer‐textured sites in both the Great Basin and the Chihuahuan deserts, while the coarser‐textured site in the Great Basin did show some recovery over time and the Chihuahuan coarser‐textured site did not. This study demonstrates the importance of understanding site potential and identifying key limitations to biocrust recovery for successful restoration projects.

A commercial arbuscular mycorrhizal inoculum increases root colonization across wheat cultivars but does not increase assimilation of mycorrhiza-acquired nutrients.

Societal Impact StatementProduction and heavy application of chemical‐based fertilizers to maintain crop yields is unsustainable due to pollution from run‐off, high CO2 emissions, and diminishing yield returns. Access to fertilizers will be limited in the future due to rising energy costs and dwindling rock phosphate resources. A growing number of companies produce and sell arbuscular mycorrhizal fungal (AMF) inoculants, intended to help reduce fertilizer usage by facilitating crop nutrient uptake through arbuscular mycorrhizas. However, their success has been variable. Here, we present information about the efficacy of a commercially available AMF inoculant in increasing AMF root colonization and fungal contribution to plant nutrient uptake, which are critical considerations within the growing AMF inoculant industry. Summary Arable agriculture needs sustainable solutions to reduce reliance on large inputs of nutrient fertilizers while continuing to improve crop yields. By harnessing arbuscular mycorrhizal symbiosis, there is potential to improve crop nutrient assimilation and growth without additional inputs, although the efficacy of commercially available mycorrhizal inocula in agricultural systems remains controversial.Using stable and radioisotope tracing, carbon‐for‐nutrient exchange between arbuscular mycorrhizal fungi and three modern cultivars of wheat was quantified in a non‐sterile, agricultural soil, with or without the addition of a commercial mycorrhizal inoculant.While there was no effect of inoculum addition on above‐ground plant biomass, there was increased root colonization by arbuscular mycorrhizal fungi and changes in community structure. Inoculation increased phosphorus uptake across all wheat cultivars by up to 30%, although this increase was not directly attributable to mycorrhizal fungi. Carbon‐for‐nutrient exchange between symbionts varied substantially between the wheat cultivars.Plant tissue phosphorus increased in inoculated plants potentially because of changes induced by inoculation in microbial community composition and/or nutrient cycling within the rhizosphere. Our data contribute to the growing consensus that mycorrhizal inoculants could play a role in sustainable food production systems of the future.

Prediction of Microbial Population in Sorghum Fermentation through Mathematical Models

The mathematical models can be used as a tool in predicting microbial population in sorghum fermentation, either spontaneous fermentation or fermentation with the addition of lactic acid bacteria (LAB) inoculum. Gompertz model modified by Gibson, Gompertz model modified by Zwietering, Baranyi-Robert model, Fujikawa model, Richards model, Schnute model were used in predicting the growth of lactic acid bacteria (LAB) and coliform bacteria during spontaneous fermentation, and also the growth of LAB during fermentation with the addition of inoculum. Meanwhile, there was death (inactivation) of coliform bacteria during sorghum fermentation with the addition of LAB inoculum. The Geeraerd model and the Gompertz model modified by Gil et al. were used to predict the inactivation. The accuracy and precision of models were evaluated based on the Root Mean of Sum Square Error (RMSE), coefficient of determination (R2), and curve fitting. Gompertz model modified by Gibson had the highest accuracy and precision, which was followed by the accuracy of the Fujikawa model and Baranyi-Robert model in predicting the growth of LAB and the growth of coliform bacteria during spontaneous fermentation. Meanwhile, in predicting LAB growth during fermentation with the addition of inoculum, high accuracy and precision was obtained from Richards and Schnute models. In predicting the inactivation of coliform bacteria, Geeraerd model provided higher accuracy and precision compared to Gompertz model modified by Gil et al. Keywords: fermentation; inoculum; mathematical; model; sorghum; spontaneous

Impact of Arbuscular Mycorrhizal Fungi on Growth and Productivity of Sugarcane Under Field Conditions

A field experiment was conducted to investigate the impact of native inoculum and inoculation with the arbuscular mycorrhizal fungus (AMF, Funneliformis mosseae KKU-BRP-KK6-2) on growth and productivity of sugarcane variety Khon Kaen 3 in the presence or absence of P fertilizer. Treatments included (1) control with native inoculum, (2) inoculation with F. mosseae with native inoculum (AMF), (3) inoculation with F. mosseae with native inoculum and with half dosage of P fertilizer (AMF + 50%F) and (4) full dosage of fertilizer (100%F) with native inoculum. Mycorrhizal colonization was significantly higher in both AMF treatments compared to the uninoculated treatments, suggesting inoculum limitation in sugarcane fields. Both of inoculation and P fertilization increased plant nutrient uptake (NPK), plant biomass and productivity as compared to the control. The highest plant biomass and productivity were observed in the AMF + 50%F treatment. Moreover, initial AMF colonization after 4 months was significantly correlated with soil properties, biomass and productivity. We conclude that inoculation with F. mosseae is an important factor to promote sugarcane productivity. Inoculum addition could also result in substantial P fertilizer reduction with 50% dose.