Addition Of H2S Research Articles

Construction and application of fluorescent probe and sensing aerogel with ability to detect hydrogen sulfide

The dyeing, wastewater acidification and anaerobic treatment processes in the textile always produce a large amount of hydrogen sulfide (H2S), which will be harmful to the human beings. The fluorescent detection of H2S with highly specific and sensitive probe is of significance. Here, using the 2, 7-dichlorofluorescein (DCF) derivative DCF-MPYM as the fluorophore, a fluorescent probe M1 for specifically recognizing H2S was designed and synthesized by introducing 2-chloro-5-nitrobenzoic acid into the oxanthracene moiety of DCF-MPYM. The introduction of 2-chloro-5-nitrobenzoic acid results in DCF-MPYM being in a closed-loop state, thus, the fluorescence quantum yield (Φ) of the probe M1 was low. The characteristic of weak fluorescence plays an important role in the use of a fluorescent probe. The fluorescence titration and selectivity results demonstrated that the probe M1 could selectively detect H2S, and its detection limit for H2S was calculated to be 45.31 μM. The reaction mechanism of the probe was verified by the high-resolution mass spectrometry (HRMS) and liquid chromatography as the nucleophilic addition of H2S to the probe M1, which induced the cleavage of ester bond and subsequently restored the spectral properties of DCF-MPYM. To bring the probe M1 into practical application, the fluorescent aerogel CMC-M1 was constructed by mixing the probe M1 and carboxymethyl cellulose sodium (CMC-Na) aerogel, and the fluorescent aerogel can be applied in absorbing and removing H2S in water. The adsorption capacity and removal efficiency of CMC-M1 for H2S were 0.626 mg·g−1 and 99.36 %. The investigation of adsorption kinetic showed that the adsorption effect of CMC-M1 towards Na2S was mainly chemical adsorption. In addition, the fluorescent aerogel CMC-M1 can be applied for qualitative, quantitative detection and effective removal of H2S in dyeing and finishing wastewater.

Multifunctional Au–Ag NCs for luminescence and colorimetric double signal sensing of H2S and catalytic reduction of nitrophenol

In this study, the N-Acetyl-l-Cysteine (NAC)-capped gold and silver bimetallic nanoclusters (NAC@Au–Ag NCs) was synthesized by reflux method. Due to the silver effect, the NAC@Au–Ag NCs exhibited strong photoluminescence at far-red/near-infrared regions and better catalytic performance than Au or Ag NCs. Upon addition of H2S, the Au–Ag NCs exhibited obvious fluorescence quench and color changes through the generation of metal sulfides and a static quenching process. The Au–Ag NCs displayed a wide linear luminescence response for H2S (18.5–217 μmol/L) with a detection limit of 0.269 μmol/L. Moreover, the visible color of Au–Ag NCs changed from white to brown-yellow along with increased H2S, the corresponding RGB values also displayed good linearity with the concentration of H2S (90.1–678 μmol/L). Notably, the fabrication of test strips provided a convenient and intuitive tool to screen the freshness of eggs by the color change of test strips. Au–Ag NCs could be used for living HeLa cells bioimaging and recognition of H2S abnormalities. Furthermore, it can be used as a catalyst to reduction of nitrophenols (NPhs), specific included 2NP, 3NP and 4NP. The reaction was regarded as a pseudo-first-order kinetic reaction due to the presence of excess NaBH4. At 298K, the catalytic rate constants(k) of 2NP, 3NP and 4NP were 0.1754 min−1, 0.1734 min−1 and 0.2782 min−1, respectively. The NAC@Au–Ag NCs catalyst still showed good catalytic activity and reusability after five cycles. Therefore, this study developed a H2S sensor for food samples and biological systems. And this nanocatalyst had great application potential for removed the nitrophenol pollutants in water.

An aggregation-induced fluorescence probe for H2S and its application in living cells

Hydrogen sulphide (H2S) plays an important role in the regulation of a number of important physiological and pathological processes. Therefore, the development of new fluorescent probes for H2S is of great importance and has recently attracted much attention. In this paper, a novel coumarin-based fluorescent probe named (1E)-1-(1-(6-chloro-2-oxo-2H-chromen-3-yl)ethylidene)thiosemicarbazide (1), which contained a CN moiety as a specific reaction site for nucleophilic addition of H2S, was prepared. Upon addition of H2S, probe 1 exhibited significant fluorescence enhancement with a detection limit as low as 0.54 μM, accompanied by a colour change from cyan to colourless. The mechanism was confirmed by ESI-MS, Job’s plot, DFT and the probe 1 was successfully applied to the imaging of H2S in HeLa cells.

Acquisition of elemental sulfur by sulfur-oxidising Sulfolobales.

Elemental sulfur (S8 0)-oxidising Sulfolobales (Archaea) dominate high-temperature acidic hot springs (>80°C, pH <4). However, genomic analyses of S8 0-oxidising members of the Sulfolobales reveal a patchy distribution of genes encoding sulfur oxygenase reductase (SOR), an S8 0 disproportionating enzyme attributed to S8 0 oxidation. Here, we report the S8 0-dependent growth of two Sulfolobales strains previously isolated from acidic hot springs in Yellowstone National Park, one of which associated with bulk S8 0 during growth and one that did not. The genomes of each strain encoded different sulfur metabolism enzymes, with only one encoding SOR. Dialysis membrane experiments showed that direct contact is not required for S8 0 oxidation in the SOR-encoding strain. This is attributed to the generation of hydrogen sulfide (H2S) from S8 0 disproportionation that can diffuse out of the cell to solubilise bulk S8 0 to form soluble polysulfides (Sx 2-) and/or S8 0 nanoparticles that readily diffuse across dialysis membranes. The Sulfolobales strain lacking SOR required direct contact to oxidise S8 0, which could be overcome by the addition of H2S. High concentrations of S8 0 inhibited the growth of both strains. These results implicate alternative strategies to acquire and metabolise sulfur in Sulfolobales and have implications for their distribution and ecology in their hot spring habitats.

Simple turn-on fluorescent probe for ultrafast and highly selective detection of hydrogen sulfide in aqueous solutions

5H-Benzimidazo[1,2-c]quinazoline-6-thione (BI–QT), was synthesized as a benzimidazole-based probe to detect H2S. BI–QT exhibits a fluorescent “turn-on” response in DMSO/H2O (9:1, HEPES 10 mM, pH 7.4) upon the addition of H2S. The BI–QT probe can determine micromolar (0–600 µM) H2S concentrations in aqueous systems, with a detection limit of 1.12 µM. Interestingly, BI–QT exhibited an ultrafast response to H2S, with maximum intensity achieved almost instantly when exposed to H2S. BI–QT is largely unaffected by pH and responds reliably over the wide 4–11 pH range, which highlights its applicability to various physiological scenarios. UV–vis, fluorescence, and 1H NMR spectroscopic analyses investigated the sensing mechanism. The practicality of the probe was demonstrated using water samples and living cells.

Effects of deep magmatic degassing and shallow seawater circulation on trace element and sulfur cycling in submarine hydrothermal systems: Insights from the Shijuligou analog, North China

Limited access to modern subseafloor sulfides hampers our understanding of the link between magmatic volatile influx and the cycling of trace elements and sulfur, as well as the effect of the subsequent shallow seawater circulation on these processes. Hence, we studied a well-preserved fossil analog of submarine hydrothermal systems – the Shijuligou volcanogenic massive sulfide (VMS) deposit from the North Qilian Mountains in North China to examine variations in elements and isotopes of subseafloor sulfides vertically.The vertical distribution of trace elements in subseafloor sulfides is strongly controlled by temperature gradients and redox states during the interaction between hot fluids and seawater beneath the paleo-seafloor. While the enrichment of elements like As, Sb, and Au (median: 1335, 43.7, and 0.30 ppm, respectively, n = 21) and negative δ34S values (mean: −3.07 ‰, n = 7) of euhedral pyrites in the jasper, along with the precipitation of high sulfidation minerals (e.g., enargite), suggest the input of magmatic volatiles into hydrothermal systems. During the shallow seawater-hydrothermal circulation, pyrites in the veined and stockwork zones exhibit distinctly elevated δ34S values (up to 15.74 ‰), accompanied by increased concentrations of wall-rock-derived elements (e.g., Cu, Ni, Si, and Ti) and low-temperature-responsive elements (e.g., Pb, Zn, and Cd). Sulfur isotopes of sulfides vary significantly from the surface to the deep ore zones, ranging from −3.36 to 19.84 ‰ (mean: 9.25 ‰, n = 37). The negative δ34S values of pyrites at the paleo-seafloor are due to the addition of H2S derived from the disproportionation of magmatic SO2. The increased δ34S values of stockwork and disseminated sulfides at depth are attributed to the progressive reduction of seawater sulfates by ferrous iron released from the alteration of fresh basalts. The trace elemental and isotopic characteristics of sulfides suggest the Shujuligou VMS deposit resembles the fossil analog of immature, subduction-related submarine hydrothermal systems.

Rapidly responsive and highly selective NIR fluorescent probe for detecting hydrogen sulfide in food samples and living cells

Hydrogen sulfide (H2S) is a pungent gas that is one of the key mediators of signal transduction in biological systems, and its presence is related to the freshness of some protein foods. Using phenothiazine derivatives as fluorophores and 2, 4-dinitrobenzene sulfonate (DNBS) fragments as reaction groups, a near-infrared (NIR) probe WX-HS for H2S identification was designed. With the addition of H2S, WX-HS appeared a strong fluorescence signal at 660 nm with short reaction time (90 s) and high sensitivity, and fluorescence state change from non-fluorescent to orange-red. In addition, WX-HS could effectively detect H2S produced during food oxidation. Based on its low cytotoxicity, the WX-HS probe further enabled the detection and imaging of H2S in A549 cells.

Highly selective near-infrared fluorescent turn-on probe with a large Stokes shift for the detection of hydrogen sulfide and imaging in live cells

The signalling molecules of hydrogen sulphide (H2S) play an important role in a variety of physiological and pathological processes, and its abnormal concentration causes Down's syndrome, Alzheimer's disease and diabetes mellitus. Thus, the development of an effective detection tool is of vital importance and challenge. In this paper, we synthesized a new “turn-on” hydrogen sulfide near-infrared fluorescent probe (DCM-H2S). Remarkably, this probe demonstrated an unanticipated level of selectivity for hydrogen sulfide (H2S) over other analytes, rendering it suitable for the precise determination of H2S in both in vitro and in vivo settings. Expectedly, the probe exhibited unexpectedly heightened selectivity for H2S compared to other analytes, making it a viable option for the specific determination of H2S in both in vitro and in vivo applications. With the addition of H2S, the probe exhibited distinct turn-on near-infrared (NIR) fluorescence intensity alterations in the DMSO/H2O system, together with a high sensitivity (LOD = 0.13 μM) and large Stokes shift (121 nm). More importantly, the probe has been successfully applied to bioimaging for real-time, dynamic tracking of exogenous and endogenous H2S. The outcomes showed that the probe (DCM-H2S) provides a rapid and effective detection tool for monitoring H2S in vivo.

Proteomic insights into the response of Halomonas sp. MNB13 to excess Mn(Ⅱ) and the role of H2S in Mn(Ⅱ) resistance

Halomonas spp. are moderately halophilic bacteria with the ability to tolerate various heavy metals. However, the role of basic cellular metabolism, particularly amino acid metabolism, has not been investigated in Halomonas spp. under excess Mn(Ⅱ). The strain Halomonas sp. MNB13 was isolated from a deep-sea ferromanganese nodule and can tolerate 80 mM Mn(Ⅱ). To comprehensively explore the mechanisms underlying its resistance to excess Mn(Ⅱ), we conducted a comparative proteome analysis. The data revealed that both 10 mM and 50 mM Mn(Ⅱ) significantly up-regulated the expression of proteins involved in Mn(Ⅱ) transport (MntE), oxidative stress response (alkyl hydroperoxide reductase and the Suf system), and amino acid metabolism (arginine, cysteine, methionine, and phenylalanine). We further investigated the role of cysteine metabolism in Mn(Ⅱ) resistance by examining the function of its downstream product, H2S. Consistent with the up-regulation of cysteine desulfurase, we detected an elevated level of H2S in Halomonas sp. MNB13 cells under Mn(Ⅱ) stress, along with increased intracellular levels of H2O2 and O2•−. Upon exogenous addition of H2S, we observed a significant restoration of the growth of Halomonas sp. MNB13. Moreover, we identified decreased intracellular levels of H2O2 and O2•− in MNB13 cells, which coincided with a decreased formation of Mn-oxides during cultivation. In contrast, in cultures containing NaHS, the residual Mn(Ⅱ) levels were higher than in cultures without NaHS. Therefore, H2S improves Mn(Ⅱ) tolerance by eliminating intracellular reactive oxygen species rather than decreasing Mn(Ⅱ) concentration in solution. Our findings indicate that cysteine metabolism, particularly the intermediate H2S, plays a pivotal role in Mn(Ⅱ) resistance by mitigating the damage caused by reactive oxygen species. These findings provide new insights into the amino acid mechanisms associated with Mn(Ⅱ) resistance in bacteria.

Construction of hybrid ionic liquid‐catalysts for the highly effective conversion of H2S by nitriles into thioamides

AbstractDescribed herein presents a kind of multisite hybrid ionic liquid‐catalysts and establishes a novel strategy for H2S utilization, which has been characterized by multiaspects to reveal internal structural characterization. Through chemical modification, the D101‐based hybrid ILs were used for the direct synthesis of thioamides from the addition of H2S and nitriles. D101‐[PMDETA][DMG] can achieve an impressive activity of quantitative conversion for benzonitrile. Reaction kinetics, adsorption thermodynamics, substrate universality, and catalyst cycling experiments have been systematically studied. A first‐order kinetic model was constructed and studied experimentally for the reaction of nitriles with H2S to produce thioamides. The internal mechanism was proposed through experiments and confirmed by DFT‐based theoretical calculations. Moreover, D101‐based hybrid ILs can be easily recovered, thus completing the cyclic process of thioamide multiplication. This work not only offers an effective method for the construction of organic‐inorganic hybrid materials but also provides a novel insight into efficient H2S conversion.

A purine-based fluorescent probe for H2S detection and imaging of cells

Hydrogen sulfide (H2S) is a gas with a toxic odor that plays an irreplaceable role in physiological activities within the mammalian body. Therefore, it is important to do the distribution and quantitative detection of H2S in mammalian cells. In this paper, a fluorescence probe (EDPH) based on purine scaffold was designed and synthesized with high sensitivity and good selectivity. H2S induced ether bond breakage in EDPH, resulting in a significant redshift of the absorption band (from 370 nm to 500 nm) with a Stokes shift of 130 nm. After the addition of H2S, the fluorescence intensity of EDPH showed a good linear correlation with the concentration of H2S, which enabled the quantitative detection of H2S with a low limit of detection (41 nM). Finally, the EDPH was applied to the cellular Hele, and the probe has good cellularity imaging capability for the detection of H2S in living systems.

A novel polymer-based probe for fluorescently ratiometric sensing of hydrogen sulfide with multiple applications

Hydrogen sulfide (H2S) as an endogenous signaling molecule, plays an irreplaceable role in many important physiological activities. It is also closely related to sewage treatment, wine quality evaluation, and food spoilage. Herein, we have successfully synthesized a novel polymer-based probe P1 for fluorescently ratiometric sensing of H2S with a high selectivity and sensitivity. By virtue of ring-opening metathesis polymerization (ROMP), P1 was prepared with the disulfide bond linked coumarin-norbornene dyad NB-SS-COU as energy donor, the aggregation-induced emission (AIE) fluorophore anchored norbornene NB-TPE as energy receptor, and the polyethylene glycol (PEG) attached norbornene NB-PEG as a hydrophilic chain. At the 400 nm excitation, P1 displays a bright red emission at 615 nm due to the efficient fluorescence resonance energy transfer (FRET) from energy donor COU to energy acceptor TPE. Upon addition of H2S, it shows strong COU-based blue emission at 473 nm for cleavage of the disulfide bond. We also constructed a smartphone sensing platform to conduct visual quantitative detection of H2S by calculating the B/R (blue/red) emission ratio values. Moreover, P1 can be successfully employed in evaluating the level fluctuations of endogenous and exogenous H2S in living cells, testing water samples/wine samples, and monitoring food freshness.

Combination of hydrophilic quinolinium group and ortho-aldehyde group accelerated thiolysis of dinitrophenyl ether: A colorimetric and turn-on fluorescent probes for the detection of hydrogen sulfide

Small-molecular fluorescent probes are currently being developed for sensing of hydrogen sulfide and are attracted great attention because of the significant roles of H2S in food safety and living organisms. We report herein an efficient approach to modify the previous reported quinolinium-phenol vinylic conjugated fluorescent probes 4NS by combination of the accelerated nucleophilic addition of H2S to an aldehyde group followed by an intramolecular fast thiolysis of dinitrophenyl ether. The modified probe 4ANS can be able to efficient sense H2S and displays typical features, with good selectivity, high sensitivity (LOD = 15 nM), “naked-eye” colorimetric and turn-on fluorescent detection, rapid response time (<3 mins), and good biocompatibility. The mechanism involving thiolysis of dinitrophenyl ether was confirmed by HRMS analysis, and the practical application to detect H2S in food spoilage and cell imaging was successfully demonstrated.

A fluorescent probe based on BODIPY for hydrogen sulfide imaging in living cells and zebrafish

A novel fluorescent probe based on 4,4-Difluoro-4-bora-3a,4a-diaza-s-indacene (BODIPY) structure as fluorophore with 7-nitro-1,2,3-benzoxadiazole (NBD) served as detection unit was designed for specific and sensitive detection of H2S in living cells and zebrafish. In this work, we prepared a novel fluorescent probe for detection of hydrogen sulfide with strong fluorescent enhancement, good biocompatible and excellent specifically detection. The probe 1 displayed emission at 580 nm with excited wavelength at 530 nm in aqueous solution which organic proportion was only 10%. This probe exhibits highly selectively and specifically to detect H2S compared with other related interferences including sulphur-amino acids, sulfite ion and cations. In the addition of H2S into the detection system, the fluorescent intensity was sharply increased about 170-fold with the limitation detection as low as 16.8 nM. The sensing mechanism was through the cleave reaction between analyte and NBD unit which was characterized by high resolution mass spectrum (HRMS). This platform also permits low biotoxicity which can successfully be utilized to evaluate the concentration level of H2S and imaging in living cells and zebrafish.

Multiple Applications of a Novel Fluorescence Probe with Large Stokes Shift and Sensitivity for Rapid H2S Detection.

Herein, a novel fluorescence probe Fla-DNP based on flavonol has been designed and synthesized for rapid, specific detection of H2S. With the addition of H2S, Fla-DNP triggered thiolysis and released Fla displaying the "turn-on" fluorescence response at 566nm, which is consistent with the reaction site predicted by calculating Electrostatic potential and ADCH charges. As an easily available H2S probe, Fla-DNP has the advantages of high selectivity, anti-interference, low detection limit (0.834μM), short response time (6min), and large Stokes shift (124nm). The sensing mechanism of H2S was determined by HRMS analysis and DFT calculation. Moreover, Fla-DNP processes a wide range of multiple applications, including the detection of H2S in environmental water samples with good recovery rates ranging from 89.6% to 102.0%, as well as tracking the production of H2S during food spoilage. Meanwhile, the probe exhibits superior biocompatibility and can not only be available used for H2S detection in living cells but be further designed as an H2S-activated CO photoreleaser, based on which it can be developed as a targeted anti-cancer drug.

D-cysteine desulfhydrase silence influences the level of sugar, organic acid, carotenoids, and polyphenols in tomato fruit

Hydrogen sulfide (H2S) plays important roles in the regulation of fruit ripening. D-cysteine desulfhydrase (DCD) is one of the key enzymes of H2S synthesis. However, whether and how DCD is involved in the regulation of tomato fruit quality is still unknown. Here, the tobacco rattle virus (TRV)-based virus-induced gene-silencing (VIGS) was used to silence DCD to explore the effect of DCD on quality regulation in postharvest tomato fruit. The results revealed that DCD silence accelerated the accumulation of fructose, glucose, soluble sugar, soluble protein, carotene, and lycopene. The expressions of the major genes (PFPP, ATPPF, FBA6; GPI, GPD1, POL2; ZDS, CCD7, CrtL-e-1, psy1, LCY1 and PDS) in sugar, acid and carotene metabolism were up-regulated in DCD-silenced tomatoes. DCD silence also decreased the content of malic acid, citric acid, ascorbic acid, total phenols, and total flavonoid by reducing the transcriptional level of their metabolism related genes (MDH, ME1, ME2; CSG, CS3, CSM; MDHAR and DHAR1; PAL5, 4CL, CHS2 and F3H). Meanwhile, the effects of DCD silence on tomato fruit were reversed by the addition of H2S. Thus, H2S might regulate the quality of postharvest tomato fruit through a DCD-dependent pathway.

Corrosion behavior of heat-resistant steel T91 in high-temperature supercritical carbon dioxide with impurity O2, SO2 or H2S

This study investigated corrosion of T91 in supercritical carbon dioxide (sCO2) with impurity of 100 ppm O2, SO2 or H2S at 550 ℃ and 15 MPa. O2 slightly increased mass gain of corroded T91, and the corrosion kinetics both in pure sCO2 and in sCO2 with O2 impurity followed parabolic law. Fe2O3 was formed in outer corrosion layer due to enhanced partial pressure of oxygen. SO2 or H2S remarkably exacerbated T91 corrosion. For test in sCO2 with SO2 impurity, outer corrosion product was magnetite, and the inner was composed of Fe-Cr spinel and FeS, different from that in pure sCO2. Addition of H2S caused an external FeS layer and internal Fe-Cr spinel. Introduction of all the three impurities reduced carburization in the underlying alloy substrate.

H2S-Enriched Flush out Does Not Increase Donor Organ Quality in a Porcine Kidney Perfusion Model

Kidney extraction time has a detrimental effect on post-transplantation outcome. This study aims to improve the flush-out and potentially decrease ischemic injury by the addition of hydrogen sulphide (H2S) to the flush medium. Porcine kidneys (n = 22) were extracted during organ recovery surgery. Pigs underwent brain death induction or a Sham operation, resulting in four groups: donation after brain death (DBD) control, DBD H2S, non-DBD control, and non-DBD H2S. Directly after the abdominal flush, kidneys were extracted and flushed with or without H2S and stored for 13 h via static cold storage (SCS) +/- H2S before reperfusion on normothermic machine perfusion. Pro-inflammatory cytokines IL-1b and IL-8 were significantly lower in H2S treated DBD kidneys during NMP (p = 0.03). The non-DBD kidneys show superiority in renal function (creatinine clearance and FENa) compared to the DBD control group (p = 0.03 and p = 0.004). No differences were seen in perfusion parameters, injury markers and histological appearance. We found an overall trend of better renal function in the non-DBD kidneys compared to the DBD kidneys. The addition of H2S during the flush out and SCS resulted in a reduction in pro-inflammatory cytokines without affecting renal function or injury markers.

A highly selective and easily acquisitive near-infrared fluorescent probe for detection and imaging of hydrogen sulfide in cells

Hydrogen sulfide (H2S) plays a substantial role as a messenger in the physiological and pathological processes of many diseases. Recently, the fluorescence probe of H2S based on organic dye has attracted great attention. However, the emission of many probes is in the UV–vis region (400–600 nm), so it has the disadvantages of shallow tissue penetration and more vulnerable to spontaneous fluorescence interference. Although several H2S probes have been developed that emit more than 650 nm, there is a complex structure difficult to synthesize or unstable in storage. Aimed at simply structural and easily synthesized H2S fluorescent probes with emission wavelength more than 650 nm, a novel near-infrared (NIR) probe (NIR-H2S) here was rationally designed with 4-(2-carboxyphenyl)-7-(diethylamino)-2-(4-hydroxystyryl)chromenylium (NIR-OH) as a fluorescent dye and 2,4-dinitrophenyl moiety as a recognition group. Addition of H2S, the “turn-on” NIR fluorescence response at 736 nm of NIR-H2S was displayed, accompanied by a visual colour change from purple to green when excited at 686 nm. As an easily acquisitive H2S probe, NIR-H2S has been successfully applied to cell imaging for H2S detection with the advantages such as long fluorescence emission, low toxicity, high sensitivity and strong selectivity.

Novel fluorescent probe based on dicoumarin for detection of hydrogen sulfide in real samples

AbstractA novel dicoumarin‐derived hydrogen sulfide (H2S) selective fluorescent “turn‐on” probe 3‐(2,4‐dinitrobenzenesulfonate)‐dicoumarin (DC‐HS) created by covalent bonding between the 2,4‐dinitrobenzenesulfonyl (DNBS) and the 3‐hydroxy‐dicoumarin (DC‐OH) units. Upon the addition of H2S, the probe DC‐HS solution's fluorescence significantly increased, and its appearance is changed from practically colorless to brilliant yellow. Probe DC‐HS also showed significant fluorescence amplification that was quantitatively detectable in the concentration range of 0–1.5 μM and had a low detection limit or limit of detection of 0.2 nM. Moreover, with a high recovery rate and excellent accuracy, the developed fluorescent molecule was used successfully for the analysis of H2S in red wine samples.