A series of active gelatin films containing natural antioxidants (NAs) were used as food packaging in this paper. Five kinds of NAs [green tea extract (GTE), grape seed extract (proanthocyanidins, OPC), grape seed extract (grape seed polyphenols, GSP), ginger extract (GE), gingko leaf extract (GBE)] were added into gelatin-based films as antioxidant additive. The effects of these extracts on the antioxidant, physical and mechanical properties of the films were studied. The results suggested that addition of GBE made the gelatin-based film possess the highest DPPH radical scavenging activity at 1.0 mg/ml. The UV light barrier was also greatly improved when the film mixed with GBE and the value of light transmission was 23.242% at 400 nm. In addition, it was observed that the gelatin-based film blending of GTE resulted in significantly increased protection against moisture. As to the mechanical properties, the lowest tensile strength (14.64 ± 2.51 MPa) and percentage of elongation at break (30.86 ± 6.86%) were found in the gelatin-based film mixing of GSP. The moisture content and water solubility of films were not altered with addition of NA. Furthermore, the active gelatin films showed the heterogeneous surface and porous formation by scanning electron microscopy after blending of NA. At the same time, the FTIR-ATR spectra was used to investigate the interactions between gelatin and extracts. These results reveal that addition of GTE, OPC, GBE into gelatin film is an ideal choice to be developed into active food packaging for food preservation.