Addition Of Dipyridamole Research Articles

The Effect of Dipyridamole on the Adenosine Triphosphate Level of Stored Human Blood

The addition of dipyridamole, a synthetic deaminase inhibitor at a concentration of 1 × 10−4 M to human Heparin‐ACD blood fortified with adenosine, results in an increase in ATP above control levels during storage at 4 C, rising to a maximum of 130 per cent at five days and falling to 20 per cent at 30 days. Aliquots of the same blood fortified with adenine, adenosine or both incubated at 37 C for 30 hours showed levels at or slightly above the upper limit of +2 SD of normal. The addition of dipyridamole at a concentration of 1 × 10−4 M to similar aliquots showed significant increases in ATP above normal, rising to a maximum of 300, 350 and 400 per cent in the aliquots fortified with adenine, adenosine and both respectively.Whereas the presence of adenine, adenosine or both in Heparin‐ACD blood did not significantly alter the rise in whole blood inorganic phosphate during incubation at 37 C for 30 hours, the addition of dipyridamole to similar aliquots depressed the level by from 20 to 30 per cent of control values.The possible beneficial role of a deaminase inhibitor in the preservation of human red cells is discussed.