Additional Level Research Articles

Storage protein activator controls grain protein accumulation in bread wheat in a nitrogen dependent manner

The expression of cereal grain storage protein (GSP) genes is controlled by a complex network of transcription factors (TFs). Storage protein activator (SPA) is a major TF acting in this network but its specific function in wheat (Triticum aestivum L.) remains to be determined. Here we generated an RNAi line in which expression of the three SPA homoeologs was reduced. In this line and its null segregant we analyzed GSP accumulation and expression of GSP and regulatory TF genes under two regimes of nitrogen availability. We show that down regulation of SPA decreases grain protein concentration at maturity under low but not high nitrogen supply. Under low nitrogen supply, the decrease in SPA expression also caused a reduction in the total quantity of GSP per grain and in the ratio of GSP to albumin-globulins, without significantly affecting GSP composition. The slight reduction in GSP gene expression measured in the SPA RNAi line under low nitrogen supply did not entirely account for the more significant decrease in GSP accumulation, suggesting that SPA regulates additional levels of GSP synthesis. Our results demonstrate a clear role of SPA in the regulation of grain nitrogen metabolism when nitrogen is a limiting resource.

Dual functional roles of nutritional additives in nutritional fortification and safety of thermally processed food: Potential, limitations, and perspectives.

The Maillard reaction (MR) has been established to be a paramount contributor to the characteristic sensory property of thermally processed food products. Meanwhile, MR also gives rise to myriads of harmful byproducts (HMPs) (e.g., advanced glycation end products (AGEs) and acrylamide). Nutritional additives have attracted increasing attention in recent years owing to their potential to simultaneously improve nutritional quality and attenuate HMP formation. In this manuscript, a brief overview of various nutritional additives (vitamins, minerals, fatty acids, amino acids, dietary fibers, and miscellaneous micronutrients) in heat-processed food is provided, followed by a summary of the formation mechanisms of AGEs and acrylamide highlighting the potential crosstalk between them. The main body of the manuscript is on the capability of nutritional additives to modulate AGE and acrylamide formation besides their traditional roles as nutritional enhancers. Finally, limitations/concerns associated with their use to attenuate dietary exposure to HMPs and future perspectives are discussed. Literature data support that through careful control of the addition levels, certain nutritional additives possess promising potential for simultaneous improvement of nutritional value and reduction of AGE and acrylamide content via multiple action mechanisms. Nonetheless, there are some major concerns that may limit their wide applications for achieving such dual functions, including influence on sensory properties of food products, potential overestimation of nutrition enhancement, and introduction of hazardous alternative reaction products or derivatives. These could be overcome through comprehensive assay of dose-response relationships and systematic evaluation of the diverse combinations from the same and/or different categories of nutritional additives to establish synergistic mixtures.

Adaptation of Anaerobic Digestion Microbial Communities to High Ammonium Levels: Insights from Strain-Resolved Metagenomics.

Ammonia release from proteinaceous feedstocks represents the main inhibitor of the anaerobic digestion (AD) process, which can result in a decreased biomethane yield or even complete failure of the process. The present study focused on the adaptation of mesophilic AD communities to a stepwise increase in the concentration of ammonium chloride in synthetic medium with casein used as the carbon source. An adaptation process occurring over more than 20 months allowed batch reactors to reach up to 20 g of NH4+ N/L without collapsing in acidification nor ceasing methane production. To decipher the microbial dynamics occurring during the adaptation and determine the genes mostly exposed to selective pressure, a combination of biochemical and metagenomics analyses was performed, reconstructing the strains of key species and tracking them over time. Subsequently, the adaptive metabolic mechanisms were delineated by following the single nucleotide variants (SNVs) characterizing the strains and prioritizing the associated genes according to their function. An in-depth exploration of the archaeon Methanoculleus bourgensis vb3066 and the putative syntrophic acetate-oxidizing bacteria Acetomicrobium sp. ma133 identified positively selected SNVs on genes involved in stress adaptation. The intraspecies diversity with multiple coexisting strains in a temporal succession pattern allows us to detect the presence of an additional level of diversity within the microbial community beyond the species level.

Valorization of Potato Peel Waste as Natural Additive for Use in Meat Products

Potato peel is a waste generated in large amounts in the food industry; however, it has been shown that these residues are an important source of antioxidant compounds. The effect of potato peel powder addition (2, 5, and 10%) on the physicochemical, sensory, and antioxidant status of pork patties during refrigerated storage (2 °C/9 days/under dark) was evaluated. Polyphenol content and antioxidant activity of potato peel powder ethanol extract were determined. Pork patties were subjected to proximate chemical composition, physicochemical, and sensory evaluations. Results showed that potato peel ethanol extract at the highest used concentration (500 µg/mL) is an important source of total phenolic (>50 mg gallic acid equivalents/g) and chlorogenic acid compounds (ca. 40 mg chlorogenic acid equivalents/g) and exerts free radical scavenging (>50% of DPPH inhibition) and reducing power activity (<0.5 abs) (p < 0.05). Additionally, potato peel powder incorporation in raw pork patties reduces changes in pH, lipid oxidation, water-holding capacity, cooking loss weight, and color values during storage. Although an effect was observed on texture and sensory values (color and appearance) of raw patties, depending on addition level (p < 0.05), no differences were found in color appearance, odor, flavor, juiciness, fat sensation, texture, and overall acceptability of cooked patties between treatments (p > 0.05). The use of potato peel powder as a natural antioxidant for meat products is recommended.

Identification of Milk Adulteration in Camel Milk Using FT-Mid-Infrared Spectroscopy and Machine Learning Models.

Camel milk, esteemed for its high nutritional value, has long been a subject of interest. However, the adulteration of camel milk with cow milk poses a significant threat to food quality and safety. Fourier-transform infrared spectroscopy (FT-MIR) has emerged as a rapid method for the detection and quantification of cow milk adulteration. Nevertheless, its effectiveness in conveniently detecting adulteration in camel milk remains to be determined. Camel milk samples were collected from Alxa League, Inner Mongolia, China, and were supplemented with varying concentrations of cow milk samples. Spectra were acquired using the FOSS FT6000 spectrometer, and a diverse set of machine learning models was employed to detect cow milk adulteration in camel milk. Our results demonstrate that the Linear Discriminant Analysis (LDA) model effectively distinguishes pure camel milk from adulterated samples, maintaining a 100% detection rate even at cow milk addition levels of 10 g/100 g. The neural network quantitative model for cow milk adulteration in camel milk exhibited a detection limit of 3.27 g/100 g and a quantification limit of 10.90 g/100 g. The quantitative model demonstrated excellent precision and accuracy within the range of 10-90 g/100 g of adulteration. This study highlights the potential of FT-MIR spectroscopy in conjunction with machine learning techniques for ensuring the authenticity and quality of camel milk, thus addressing concerns related to food integrity and consumer safety.

Changes in the physical properties of the meat of broiler carcasses using aloe vera oil extracted locally compared to the imported

This study was conducted to demonstrate the changes in the physical properties of broiler carcasses by locally extracted aloe vera oil compared to the imported, from 3/5/2022 to 5/6/2022. 270 unsexed Ross 308 broiler chicks, one day old, with 4 treatments were. T1 (control treatment), the locally extracted aloe vera oil treatments were T2 and T3 (water bath), T4 and T5 (oil immersion). The treatments of imported aloe vera oil were T6 and T7 (Pakistani origin), T8 and T9 (Iranian origin). Addition levels were 0.30 and 0.40 ml oil/kg deit. The physical characteristics of the studied broiler carcasses meat were Water Holding Capacity (WHC), Thawing loss, Drip loss and Cooking loss. A significant improvemnt (P≤0.05) for treatments of aloe vera oil extracted locally, especially extracted by the water bath method, in the physical characteristics of the meat of broiler carcasses, represented by each of the water holding capacity, the effluent liquid and the loss when melting and cooking, compared with imported aloe vera oil and control treatment. T3 treatment was the best on other locally extracted aloe vera oil treatments.

Impact of different levels of foamed sulfur and Nitrogen fertilizer (Urea) on soil reaction (pH) and yield of wheat (Triticum aestivum L.) cultivar (IBAA 99)

An experiment was conducted in one of the agricultural lands of Al-Muthanna Governorate for the agricultural season 2018_2019, to find out the effect of adding one of the soil conditioners represented by foamed sulfure by three levels (0, 1000 and 2000) kg S ha-1 , addition of nitrogen fertilizer (Urea) CO(NH2)2 at three levels (200, 400 and 600) kg N ha-1 to the soil in the degree of soil reaction (pH) and the yield of wheat cultivar (IPAA 99). The treatments within the experimental units were arranged according to the Randomized Complete Block Design (RCBD) with four replicates, so the total experimental units were 36 experimental units, where the level of addition of 2000 kg N ha-1 of foamed sulfur and the level of 600 kg N ha-1 of nitrogen fertilizer (Urea) were significantly higher, to give them the lowest averages for the soil reaction (pH) of 7.13 and 7.38 at germination and 7.19 and 7.29 at the flowering stage for each of the sulfur and nitrogen fertilizers, respectively, compared to the comparison treatment. Both levels were significantly superior in giving them the highest averages for some growth characteristics, and the yield amounted to(343 and 304 spikes m-2 , 65 and 62 grains of spike-2 , 43.58 and 42.20 gm, and 4.88 and 478 tons ha-1 and 25.60 and 24.32%, for the characteristics spikes numbers, the grains spike number, 1000 grain weights, grains yield and the protein content percentage in grains, respectively, for both levels. Keywords: foamed sulfur, Nitrogen fertilizer (Urea), soil reaction (pH), yield, wheat (Triticum aestivum L.), IBAA 99cultivar.

Effect of oat bran fiber on physicochemical properties and acceptance of enriched rabbit meat burgers

Burger is a practical source of animal protein for consumers, sold both raw (ready-to-cook) and cooked (ready-to-eat), and can be an alternative to stimulate the consumption of rabbit meat, which is a source of healthy animal protein. This study evaluated the effects of different levels of oat bran addition on physicochemical properties and sensory acceptance of rabbit meat burgers. Burgers were formulated with 0, 5, 10, and 15% oat bran plus garlic and salt, then manually processed and analyzed for composition, technological parameters (pH, cooking loss, shrinkage percentage, water holding, texture, and color), and sensorial acceptance. The higher the levels of inclusion of oat bran, the lower the burger moisture (p < 0.0001). Burgers with 15% oat bran presented higher content of lipids and carbohydrates, thus higher caloric value (p < 0.0001). The loss of water during cooking was greater in the burger without the inclusion of oat bran (p < 0.05). No significant difference was found for color and aroma attributes. Overall acceptability was higher for burgers with 5% oat bran. Rabbit meat burgers containing oat bran may represent a viable alternative to improving their sensory and technological profiles and overall consumer acceptance.

Combination therapy of metformin and morin attenuates insulin resistance, inflammation, and oxidative stress in skeletal muscle of high-fat diet-fed mice.

Lipid accumulation, inflammation, and oxidative stress are the most important causes of muscle insulin resistance. The aim of this study was to investigate the single and combined treatment effects of metformin (MET) and morin (MOR) on lipid accumulation, inflammation, and oxidative stress in the skeletal muscle of mice fed a high-fat diet. The mice were supplemented with MET (230 mg/kg diet), MOR (100 mg/kg diet), and MET + MOR for 9 weeks. Our results revealed that single treatment with MET or MOR, and with a stronger effect of MET + MOR combined treatment, reduced body weight gain, improved glucose intolerance and enhanced Akt phosphorylation in the muscle tissue. In addition, plasma and muscle triglyceride levels were decreased after treatment with MET and MOR. The expression of genes involved in macrophage infiltration and polarization and pro-inflammatory cytokines showed that MET + MOR combined treatment, significantly reduced inflammation in the muscle. Furthermore, combined treatment of MET + MOR with greater efficacy than the single treatment improved several oxidative stress markers in the muscle. Importantly, combined treatment of MET and MOR could increase the expression of nuclear factor erythroid 2-related factor 2, the master regulator of the antioxidant response. These findings suggest that combination of MET with MOR might ameliorate insulin resistance, inflammation, and oxidative stress in the skeletal muscle of mice fed high-fat diet.

Preparation and thermal shock resistance of solar thermal storage ceramics from high calcium and high iron steel slag

To guarantee the efficiency of solar thermal power generation, the solar thermal storage material is required to have excellent thermal shock resistance to withstand the process of long-term thermal cycles. In this study, solar thermal storage ceramics were prepared using high calcium and high iron steel slag as the main raw material, adding cordierite and using the pressureless sintering method. The effects of cordierite addition on the physical properties, phase composition, microstructure, thermal storage and thermal shock resistance of the samples were investigated by XRD, SEM and so on. The results show that the addition of cordierite promotes densification and improves thermal shock resistance. The optimal level of addition is 10 %. When the temperature is 1240 °C, the bulk density and bending strength of the B5 (10 wt% cordierite) are 3.03 g cm−3 and 92.52 MPa, respectively. The bending strength of B5 after 30 thermal shock cycles is 97.06 MPa, which is 4.91 % higher than that before 30 thermal shock cycles. The thermal storage density is 867.95 kJ kg−1. This work not only analyzes the feasibility of this material for high-temperature service but also provides a new way for high-value utilization of high calcium and high iron steel slag.

Differential Toxicity Responses between Hepatopancreas and Gills in Litopenaeus vannamei under Chronic Ammonia-N Exposure.

Ammonia nitrogen is one of the main toxic substances in aquatic cultivation environments. Chronic exposure to excessive amounts of ammonia-N creates toxic consequences, retarding the growth of aquatic organisms. This study investigated the growth performance, morphological and physiological alterations, and transcriptome changes in the hepatopancreas and gills of white shrimp Litopenaeus vannamei. The results showed that there was no significant difference in the survival rate (p > 0.05), whereas growth performance was reduced significantly in the treated groups compared to the control groups (p < 0.05). Significant structural damage and vacuolation occurred in hepatopancreas and gill tissues in the treated groups. Superoxide dismutase (SOD) activity and Na+/K+-ATPase content were significantly increased by chronic ammonia-N exposure in the two tissue groups. In addition, catalase (CAT) activity and malondialdehyde (MDA) levels were significantly altered in the hepatopancreas groups (p < 0.05), whereas no differences were observed in the gill groups (p > 0.05). There were 890 and 1572 differentially expressed genes identified in the hepatopancreas (treated versus control groups) and gills (treated versus control groups), respectively, of L. vannamei under chronic ammonia-N exposure. Functional enrichment analysis revealed associations with oxidative stress, protein synthesis, lipid metabolism, and different serine proteases. The gills maintained cellular homeostasis mainly through high expression of cytoskeleton and transcription genes, whereas the hepatopancreas down-regulated related genes in the ribosome, proteasome, and spliceosome pathways. These genes and pathways are important in the biosynthesis and transformation of living organisms. In addition, both tissues maintained organismal growth primarily through lipid metabolism, which may serve as an effective strategy for ammonia-N resistance in L. vannamei. These results provided a new perspective in understanding the mechanisms of ammonia-N resistance in crustaceans.

Effects of rapeseed protein addition on soybean protein-based textured protein produced by low-moisture extrusion: Changes in physicochemical attributes, structural properties and barrel flow behaviors

This study investigated the potential of rapeseed protein as a novel alternative protein source in meat analogs produced by extrusion cooking technology. To study the substitutability of rapeseed protein to soybean protein, the addition ratios of rapeseed protein to soybean protein in the extrusion experiment were 0:50, 10:40, 20:30, 30:20, 40:10 and 50:0 (w/w), respectively. The results showed that adding 0–20% rapeseed protein not only decreased the specific mechanical energy and mass flow rate of the mixture during extrusion, but also enhanced the hardness and chewiness of the extrudates while reducing their elasticity and resilience. In addition, increasing the amount of rapeseed protein may lead to decreased expansion characteristics, internal pore structure, and water absorption rate of the extrudates, as well as a decrease in brightness and an increase in redness on the surface. Weibull model could well describe the rehydration behavior for dynamic fitting of the extrusion rehydration process. Moreover, with the increase of rapeseed protein substitution, the content of disulfide bonds, and the proportion of relatively ordered protein secondary structures with high stability increased, and the degree of protein denaturation decreased. Besides, the degree of starch gelatinization reduced and the thermal stability of extrudates improved. Interaction analysis indicated that disulfide bonds are the primary force maintaining protein-mediated interactions in the extrudates. The incorporation of rapeseed protein shortened the mean residence time of molten feedstock and shifted the flow state to plug flow. Overall, our investigation suggests that within low levels of addition (≤20%, w/w), rapeseed protein has the potential to partially substitute for the soybean protein-based textured proteins. High substitution levels or complete substitution are not feasible due to the relatively low expansion and rehydration rates.

Three-dimensional cell cultivation and bioprinting

The paper presents approaches to the formation of flat and three-dimensional cellular patterns using bioprinting methods, discusses the features of three-dimensional culture, when due to more intensive intercellular interactions the cell ensemble has properties that are not typical for single cells, the conditions necessary for the emergence of self-organization processes are created in the system of interacting cells, transformation into tissue-like and organ-like structures. Modern bioprinting methods provide increased productivity of these processes by engineering the additional levels of organization.

Aberrant Behavior of Deoxynivalenol Production in the Malting of Fusarium Head Blight Infected Barley: Mycotoxin Accumulation and Hyphal Localization in Single Kernels

The malting industry faces a dilemma in which barley, initially containing low deoxynivalenol levels, occasionally exhibits an unexpected increase in deoxynivalenol levels during malting. In the current study, we investigated mycotoxin accumulation and hyphal localization in single kernels from Fusarium head blight infected barley and malt samples which showed an aberrant behavior of deoxynivalenol increases during malting. While deoxynivalenol levels in these bulk barley samples already ranged from <0.20 to 1.27 µg/g, they further increased on average by approximately 500% after malting, along with Fusarium growth. In addition, deoxynivalenol-3-glucoside and 3-acetyl deoxynivalenol levels increased from <0.20 µg/g to the mean levels of 2.47 µg/g and 1.03 µg/g in the bulk malt, respectively. In the investigation of single kernels, results showed that only 7% of barley single kernels (n = 385) had deoxynivalenol >1.00 μg/g, with the maximum level of 35.97 μg/g. However, 31% of malt single kernels contained deoxynivalenol >1.00 μg/g and up to 255.43 μg/g. Deoxynivalenol-3-glucoside and 3-acetyl deoxynivalenol were found in 40% of malt single kernels. Fungal hyphae were observed in the aleurone layer and embryo of barley kernels with deoxynivalenol >10.00 μg/g, in addition to the husk and vascular bundles of kernels with deoxynivalenol <1.00 μg/g. Hyphae spread largely in these tissues following malting, and even into the endosperm and pericarp cavities of extremely high deoxynivalenol kernels (i.e., >100.00 μg/g).

Glycerol and reuterin-producing Limosilactobacillus reuteri enhance butyrate production and inhibit Enterobacteriaceae in broiler chicken cecal microbiota PolyFermS model

BackgroundAdministering probiotic strains of Limosilactobacillus reuteri to poultry has been shown to improve poultry performance and health. Some strains of L. reuteri taxa can produce reuterin, a broad-spectrum antimicrobial compound from glycerol conversion, with high inhibitory activity against enterobacteria. However, little is known about the metabolism of glycerol in the complex chicken cecal microbiota nor the effect of glycerol, either alone or combined with L. reuteri on the microbiota. In this study, we investigated the effect of L. reuteri PTA5_F13, a high-reuterin-producing chicken strain and glycerol, alone or combined, on broiler chicken cecal microbiota composition and activity using the continuous PolyFermS model recently developed to mimic chicken cecal fermentation.MethodsThree independent PolyFermS chicken cecal microbiota models were inoculated with immobilized cecal microbiota from different animals and operated continuously. The effects of two additional levels of glycerol (50 and 100 mM) with or without daily supplementation of chicken-derived L. reuteri PTA5_F13 (107 CFU/mL final concentration) were tested in parallel second-stage reactors continuously inoculated with the same microbiota. We analyzed the complex chicken gut microbiota structure and dynamics upon treatment using 16S rRNA metabarcoding and qPCR. Microbiota metabolites, short-chain and branched-chain fatty acids, and glycerol and reuterin products were analyzed by HPLC in effluent samples from stabilized reactors.ResultsSupplementation with 100 mM glycerol alone and combined with L. reuteri PTA5_F13 resulted in a reproducible increase in butyrate production in the three modelled microbiota (increases of 18 to 25%). Glycerol alone resulted also in a reduction of Enterobacteriaceae in two of the three microbiota, but no effect was detected for L. reuteri alone. When both treatments were combined, all microbiota quantitatively inhibited Enterobacteriaceae, including in the last model that had very high initial concentrations of Enterobacteriaceae. Furthermore, a significant 1,3-PDO accumulation was measured in the effluent of the combined treatment, confirming the conversion of glycerol via the reuterin pathway. Glycerol supplementation, independent of L. reuteri addition, did not affect the microbial community diversity.ConclusionsGlycerol induced a stable and reproducible butyrogenic activity for all tested microbiota and induced an inhibitory effect against Enterobacteriaceae that was strengthened when reuterin-producing L. reuteri was spiked daily. Our in vitro study suggests that co-application of L. reuteri PTA5_F13 and glycerol could be a useful approach to promote chicken gut health by enhancing metabolism and protection against Enterobacteriaceae.

Investigating on the microstructure and optical properties of Au, Ag and Cu implanted TiN thin films: The effects of surface oxidation and ion-induced defects

In the present paper, the effects of metal ion implantation on the structural and optical properties of TiN thin films have been investigated. TiN films of 170 nm thickness were grown by d.c. reactive sputtering on Si (100) wafers and then irradiated at 5 × 1016 ions/cm2 with either Au, Ag, or Cu ions by using two different energies per each implanted metal. The results showed that as deposited TiN crystallizes in the form of a fcc cubic structure, with the crystallites preferentially oriented along the (111) plane. For all implanted layers, the cubic structure was preserved, but compared to as deposited TiN the crystallites were smaller and the lattice was contracted. These changes were correlated with the depth distribution of Au, Ag and Cu ions and assigned to implantation-induced damage that was larger when higher ion energies were used. High-resolution XPS spectra of the surface of as deposited sample showed the coexistence of TiN, TiO2 and TiOxNy phases and this was related to the surface oxidation of the films due to the exposure to air. After implantation, the results were almost similar for all metals, showing an increase in TiO2 contribution and the formation of pure metallic Au and Ag phases, while copper is in the Cu2+ state, which is attributed to Cu(II)-oxide and Cu(OH)2. The microstructural characteristics including defect formation, changes in crystallite size and lattice contraction, and also growth of different metallic phases during implantations were correlated with the findings of the optical characterization of the implanted films. For the as deposited film we found an energy gap of 2.91 eV, which was lower than the value typical for TiN. After implantation the gap was shifted to higher energies, while at the visible part of the region, the existence of additional energy levels, at photon energies below 2.9 eV was observed. Besides, all implanted films showed degraded photocatalytic activity compared to as deposited TiN, among which Cu-implanted samples exhibited the best photocatalytic performances. The lower photocatalytic activity of Au and Ag implanted films compared to Cu implantations was ascribed to larger structural defects and the formation of less favorable electronic states.

Pasta fortified with β-glucan Isolated from brewer’s yeast (Saccharomyces cerevisiae) by-product

β-glucan from brewer's spent yeast is a novel ingredient approved to be included in the human diet. This paper evaluated the effects of β-glucan extracted from spent brewer's yeast on the physico-chemical, rheological, textural, microstructure, and molecular characteristics of common and durum wheat pasta, at different addition levels. The results showed a decrease in dough viscous and elastic moduli, while the resistance to deformation and hardness raised as the addition level increased. Pasta quality parameters were influenced by the β-glucan content depending on the wheat type, with lower pasta firmness (2.76 and 3.40 N for common and durum controls vs. 2.76–1.97 and 3.35–2.73 N for common and durum enriched pasta respectively), increased carbohydrate content (from 85.63% to 85.91–86.95% in common wheat pasta, and from 81.89 to 82.12–82.28% in durum pasta), cooking loss (from 4.73% in common control to 4.91% for 4% common wheat-β-glucan pasta, and from 3.68 in durum control to 5.05% for 4% durum wheat-β-glucan), and darker color being obtained. Pasta microstructure and molecular FT-IR characteristics confirmed the presence of β-glucan in cooked pasta and depicted protein denaturation and contribution to the final product matrix. These results represent new insights into the development of convenient matrices for functional ingredient incorporation.

The Effect of Tillage Systems, Type of Organic Fertilizer and the Level of Binary Addition of Nitrogen and Phosphorus on the Growth and Yield of Clover in Gypsum Soil

A field experiment was conducted at the research station of the College of Agriculture - University of Tikrit for the agricultural season (2021-2022), and the experiment factors were three, namely: the first factor is tillage systems, as two systems of tillage were applied (no-tillage and Reduced tillage) and the second factor was the type of organic fertilizer and it included (Sheep waste and waste Mushroom), while the third factor was the level of binary addition of nitrogen and phosphorus, and it included three levels of fertilization (0%, 100%, and 125%), according to the fertilizer recommendation. The experiment was carried out according to the split-split plot system with a randomized complete block design (R.C.B.D) and with three replications. The treatments were distributed as follows: tillage systems (NT, RT) in the main plot, and the application of the type of organic fertilizer (O1, O2) In the sub plot, the levels of binary addition of nitrogen and phosphorus (M0, M1, M2) were placed in the sub-sub plots, and the results of the experiment showed that the system of Reduced tillage was significantly superior to the system of no-tillage in all the studied traits, and sheep waste was significantly superior to mushroom waste in All the characteristics of the study, as well as the level of binary addition M2 was significantly superior to the other levels in all the characteristics of the study. The results showed that the treatment (T1O1M2) was significantly superior in all traits of vegetative growth and yield traits of clover plant.

Sugarcane/soybean intercropping with reduced nitrogen addition promotes photosynthesized carbon sequestration in the soil.

Sugarcane/soybean intercropping with reduced nitrogen (N) addition has improved soil fertility and sustainable agricultural development in China. However, the effects of intercropping pattern and N fertilizer addition on the allocation of photosynthesized carbon (C) in plant-soil system were far less understood. In this study, we performed an 13CO2 pulse labeling experiment to trace C footprints in plant-soil system under different cropping patterns [sugarcane monoculture (MS), sugarcane/soybean intercropping (SB)] and N addition levels [reduced N addition (N1) and conventional N addition (N2)]. Our results showed that compared to sugarcane monoculture, sugarcane/soybean intercropping with N reduced addition increased sugarcane biomass and root/shoot ratio, which in turn led to 23.48% increase in total root biomass. The higher root biomass facilitated the flow of shoot fixed 13C to the soil in the form of rhizodeposits. More than 40% of the retained 13C in the soil was incorporated into the labile C pool [microbial biomass C (MBC) and dissolved organic C (DOC)] on day 1 after labeling. On day 27 after labeling, sugarcane/soybean intercropping with N reduced addition showed the highest 13C content in the MBC as well as in the soil, 1.89 and 1.14 times higher than the sugarcane monoculture, respectively. Moreover, intercropping pattern increased the content of labile C and labile N (alkaline N, ammonium N and nitrate N) in the soil. The structural equation model indicated that the cropping pattern regulated 13C sequestration in the soil mainly by driving changes in labile C, labile N content and root biomass in the soil. Our findings demonstrate that sugarcane/soybean intercropping with reduced N addition increases photosynthesized C sequestration in the soil, enhances the C sink capacity of agroecosystems.

A Data Science Maturity Model Applied to Students' Modeling

Maturity models define a series of levels, each representing an increased complexity in information systems. Data Science appears in the Business Intelligence (BI) and Business Analytics (BA) literature. This work applies the _IABE maturity model, which includes two additional levels: Data Engineering (DE) at the bottom and Business Experimentation (BE) at the top. This study uses the _IABE model for students' modeling in the ModEst project. For this purpose, the Public Administration organism is the Directorate-General for Statistics of Education and Science (DGEEC) of the Portuguese Education Ministry. DGEEC provided vast data on two million students per year in the Portuguese school system, from pre-scholar to doctoral programs. This work presents the comprehensible _IABE maturity model to extract new knowledge from the DGEEC dataset. The method applied is _IABE, where after the DE level, wh-questions are formulated and answered with the most appropriate techniques at each maturity level. This work's novelty is applying the maturity model _IABE to a unique dataset for the first time. Wh-questions are stated at the BI level using data summarization; at the BA level, predictive models are performed, and counterfactual approaches are presented at the BE level. Doi: 10.28991/ESJ-2023-07-06-07 Full Text: PDF